PT777747E - Metodo de sequenciacao nucleotidica - Google Patents

Metodo de sequenciacao nucleotidica

Info

Publication number
PT777747E
PT777747E PT95929169T PT95929169T PT777747E PT 777747 E PT777747 E PT 777747E PT 95929169 T PT95929169 T PT 95929169T PT 95929169 T PT95929169 T PT 95929169T PT 777747 E PT777747 E PT 777747E
Authority
PT
Portugal
Prior art keywords
nucleotide
pct
target sample
primers
ddntps
Prior art date
Application number
PT95929169T
Other languages
English (en)
Inventor
Gillian Tully
Kevin Sullivan
Original Assignee
Sec Dep Home Dept
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sec Dep Home Dept filed Critical Sec Dep Home Dept
Publication of PT777747E publication Critical patent/PT777747E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
PT95929169T 1994-08-25 1995-08-21 Metodo de sequenciacao nucleotidica PT777747E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9417211A GB9417211D0 (en) 1994-08-25 1994-08-25 Nucleotide sequencing method

Publications (1)

Publication Number Publication Date
PT777747E true PT777747E (pt) 2002-05-31

Family

ID=10760409

Family Applications (1)

Application Number Title Priority Date Filing Date
PT95929169T PT777747E (pt) 1994-08-25 1995-08-21 Metodo de sequenciacao nucleotidica

Country Status (10)

Country Link
US (1) US5925520A (pt)
EP (1) EP0777747B1 (pt)
AT (1) ATE210191T1 (pt)
AU (1) AU3263095A (pt)
DE (1) DE69524425T2 (pt)
DK (1) DK0777747T3 (pt)
ES (1) ES2169143T3 (pt)
GB (1) GB9417211D0 (pt)
PT (1) PT777747E (pt)
WO (1) WO1996006187A1 (pt)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
EP0886681A1 (en) 1996-03-04 1998-12-30 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5965363A (en) 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
CA2268740C (en) 1996-11-06 2010-07-20 Sequenom, Inc. High density immobilization of nucleic acids
US7285422B1 (en) 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
EP0963443B1 (en) 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
EP1218541B1 (en) 1999-07-26 2008-12-10 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6107061A (en) * 1999-09-18 2000-08-22 The Perkin-Elmer Corporation Modified primer extension reactions for polynucleotide sequence detection
US6518024B2 (en) * 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
US6762018B1 (en) 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
US7435541B2 (en) 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
CA2409774A1 (en) * 2000-05-23 2001-11-29 Variagenics, Inc. Methods for genetic analysis of dna to detect sequence variances
EP1373561B1 (en) 2000-06-13 2009-02-18 The Trustees of Boston University Use of mass-matched nucleotides in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
EP1332000B1 (en) 2000-10-30 2012-06-20 Sequenom, Inc. Method for delivery of submicroliter volumes onto a substrate
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
ES2180416B1 (es) * 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
US20030143556A1 (en) * 2001-04-03 2003-07-31 Gary Blackburn Nucleic acid reactions using labels with different redox potentials
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US8304184B2 (en) 2002-07-30 2012-11-06 Baback Gharizadeh Genotyping using multiple variant-specific primer pools
EP1611169B1 (en) 2003-03-21 2010-06-02 Dow Global Technologies Inc. Morphology controlled olefin polymerization process
US20040209259A1 (en) * 2003-04-16 2004-10-21 Quest Diagnostics Investments Incorporated Methods of determining genotypes in duplicated genes and genomic regions
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US6953764B2 (en) 2003-05-02 2005-10-11 Dow Global Technologies Inc. High activity olefin polymerization catalyst and process
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
JP4810533B2 (ja) 2004-05-24 2011-11-09 アイビス バイオサイエンシズ インコーポレイティッド ディジタルスレショルド化による選択的イオン濾過作用を用いた質量分光測定法
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
CA2600184A1 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
EP1803747A1 (en) 2005-12-30 2007-07-04 Borealis Technology Oy Surface-modified polymerization catalysts for the preparation of low-gel polyolefin films
ES2567141T3 (es) 2006-05-05 2016-04-20 Dow Global Technologies Llc Complejos de hafnio de ligandos de imidazol sustituidos con carbazolilo
US9061901B2 (en) 2006-07-19 2015-06-23 Bionano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
EP2064332B1 (en) 2006-09-14 2012-07-18 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
WO2008121828A2 (en) 2007-03-28 2008-10-09 Bionanomatrix, Inc. Methods of macromolecular analysis using nanochannel arrays
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US20090180931A1 (en) 2007-09-17 2009-07-16 Sequenom, Inc. Integrated robotic sample transfer device
TW200932762A (en) 2007-10-22 2009-08-01 Univation Tech Llc Polyethylene compositions having improved properties
TW200936619A (en) 2007-11-15 2009-09-01 Univation Tech Llc Polymerization catalysts, methods of making, methods of using, and polyolefin products made therefrom
CA2729159C (en) 2008-06-30 2020-01-14 Bionanomatrix, Inc. Methods and devices for single-molecule whole genome analysis
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US9181578B2 (en) * 2008-11-18 2015-11-10 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
JP5661645B2 (ja) 2009-01-08 2015-01-28 ユニベーション・テクノロジーズ・エルエルシー ポリオレフィン重合プロセス用の添加剤
WO2010080871A1 (en) 2009-01-08 2010-07-15 Univation Technologies, Llc Additive for gas phase polymerization processes
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
WO2011008972A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systems for bioagent identification
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
BRPI1015361A2 (pt) 2009-07-23 2018-02-20 Univation Tech Llc sistema de reação de polimerização
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US8598283B2 (en) 2010-02-18 2013-12-03 Univation Technologies, Llc Methods for operating a polymerization reactor
JP5727523B2 (ja) 2010-02-22 2015-06-03 ユニベーション・テクノロジーズ・エルエルシー 触媒系及びポリオレフィン製品を製造するための該触媒系の使用方法
EP2558531B1 (en) 2010-04-13 2017-02-15 Univation Technologies, LLC Polymer blends and films made therefrom
BR112013000679A2 (pt) 2010-07-16 2016-05-31 Univation Tech Llc sistemas e métodos para medir acúmulo de partícula em superfícies de reator
WO2012009215A1 (en) 2010-07-16 2012-01-19 Univation Technologies, Llc Systems and methods for measuring static charge on particulates
WO2012015898A1 (en) 2010-07-28 2012-02-02 Univation Technologies, Llc Systems and methods for measuring velocity of a particle/fluid mixture
EP2651982B1 (en) 2010-12-17 2018-04-11 Univation Technologies, LLC Systems and methods for recovering hydrocarbons from a polyolefin purge gas product
CN103347905B (zh) 2010-12-22 2015-12-16 尤尼威蒂恩技术有限责任公司 用于聚烯烃聚合方法的添加剂
WO2012159089A1 (en) 2011-05-19 2012-11-22 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
WO2013028283A1 (en) 2011-08-19 2013-02-28 Univation Technologies, Llc Catalyst systems and methods for using same to produce polyolefin products
WO2013070602A1 (en) 2011-11-08 2013-05-16 Univation Technologies, Llc Methods for producing polyolefins with catalyst systems
SG11201404696VA (en) 2012-03-05 2014-12-30 Univation Tech Llc Methods for making catalyst compositions and polymer products produced therefrom
CA2800056A1 (en) 2012-12-24 2014-06-24 Nova Chemicals Corporation Polyethylene blend compositions
WO2014106143A1 (en) 2012-12-28 2014-07-03 Univation Technologies, Llc Supported catalyst with improved flowability
EP2953985B1 (en) 2013-02-07 2023-06-07 Univation Technologies, LLC Preparation of polyolefin
ES2644669T3 (es) 2013-03-15 2017-11-29 Univation Technologies, Llc Ligandos para catalizadores
EP2970524B1 (en) 2013-03-15 2018-03-07 Univation Technologies, LLC Tridentate nitrogen based ligands for olefin polymerisation catalysts
BR112015029498B1 (pt) 2013-06-05 2020-10-27 Univation Technologies, Llc método para proteger um grupo fenol em um composto precursor e método para gerar um polímero de polietileno
CA2957809C (en) 2014-08-19 2023-01-03 Univation Technologies, Llc Fluorinated catalyst supports and catalyst systems
SG11201701265UA (en) 2014-08-19 2017-03-30 Univation Tech Llc Fluorinated catalyst supports and catalyst systems
US10208139B2 (en) 2014-08-19 2019-02-19 Univation Technologies, Llc Fluorinated catalyst supports and catalyst systems
CA2974122C (en) 2015-01-21 2023-09-19 Univation Technologies, Llc Methods for gel reduction in polyolefins
EP3247548B1 (en) 2015-01-21 2021-09-01 Univation Technologies, LLC Methods for controlling polymer chain scission
CA2983224A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
WO2016172579A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc, Multiplex methods for detection and quantification of minor variants
EP3091083B1 (en) * 2015-05-07 2017-11-08 Latvian Biomedical Research and Study Centre A kit for detecting mutation or polymorphism in the human mitochondrial dna
CN107660216B (zh) 2015-05-08 2021-05-14 埃克森美孚化学专利公司 聚合方法
WO2018063764A1 (en) 2016-09-27 2018-04-05 Exxonmobil Chemical Patents Inc. Polymerization process
WO2018063765A1 (en) 2016-09-27 2018-04-05 Exxonmobil Chemical Patents Inc. Polymerization process
EP3519466B1 (en) 2016-09-27 2023-07-05 Univation Technologies, LLC Method for long chain branching control in polyethylene production
WO2018063767A1 (en) 2016-09-27 2018-04-05 Exxonmobil Chemical Patents Inc. Polymerization process
WO2018118155A1 (en) 2016-12-20 2018-06-28 Exxonmobil Chemical Patents Inc. Polymerization process
CA3052770C (en) 2017-02-07 2021-09-07 Exxonmobil Chemical Patents Inc. Processes for reducing the loss of catalyst activity of a ziegler-natta catalyst
US11193008B2 (en) 2017-04-10 2021-12-07 Exxonmobil Chemical Patents Inc. Methods for making polyolefin polymer compositions
US10947329B2 (en) 2017-05-10 2021-03-16 Univation Technologies, Llc Catalyst systems and processes for using the same
CA3062075C (en) * 2017-05-11 2024-04-02 Gen-Probe Incorporated Compositions and methods for isolating target nucleic acids
WO2019118073A1 (en) 2017-12-13 2019-06-20 Exxonmobil Chemical Patents Inc. Deactivation methods for active components from gas phase polyolefin polymerization process
WO2019173030A1 (en) 2018-03-08 2019-09-12 Exxonmobil Chemical Patents Inc. Methods of preparing and monitoring a seed bed for polymerization reactor startup
GB201804642D0 (en) 2018-03-22 2018-05-09 Inivata Ltd Methods of labelling nucleic acids
EP3788081A1 (en) 2018-05-02 2021-03-10 ExxonMobil Chemical Patents Inc. Methods for scale-up from a pilot plant to a larger production facility
CN112055720B (zh) 2018-05-02 2022-11-22 埃克森美孚化学专利公司 从中试装置放大到较大生产设施的方法
WO2022020025A1 (en) 2020-07-22 2022-01-27 Exxonmobil Chemical Patents Inc. Polyolefin compositions and articles thereof
WO2022126068A1 (en) 2020-12-08 2022-06-16 Exxonmobil Chemical Patents Inc. High density polyethylene compositions with long-chain branching

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
DE69322266T2 (de) * 1992-04-03 1999-06-02 Perkin Elmer Corp Proben zusammensetzung und verfahren
AU8126694A (en) * 1993-10-26 1995-05-22 Affymax Technologies N.V. Arrays of nucleic acid probes on biological chips

Also Published As

Publication number Publication date
EP0777747B1 (en) 2001-12-05
WO1996006187A1 (en) 1996-02-29
GB9417211D0 (en) 1994-10-12
ATE210191T1 (de) 2001-12-15
DE69524425D1 (de) 2002-01-17
DE69524425T2 (de) 2002-08-01
ES2169143T3 (es) 2002-07-01
EP0777747A1 (en) 1997-06-11
US5925520A (en) 1999-07-20
AU3263095A (en) 1996-03-14
DK0777747T3 (da) 2002-04-08

Similar Documents

Publication Publication Date Title
PT777747E (pt) Metodo de sequenciacao nucleotidica
PL278176A1 (en) Method of detecting sequences of nucleotides and sets for carrying out diagnostic tests
ES2093633T3 (es) Amplificacion de acidos nucleicos utilizando un unico cebador.
SE9400522D0 (sv) Method and reagent for detecting specific nucleotide sequences
DE69029726D1 (de) Festphasendiagnose von medizinischen konditionen
ATE339514T1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE69430909T2 (de) Dns-sequenzbestimmung durch massenspektrometrie auf dem weg des abbaus mit exonuklease
FI950487A (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi
DE69924140D1 (de) Bestimmung der länge von repetitiven nukleinsäuresequenzen durch eine diskontinuierliche verlängerung von startermolekülen
ATE111961T1 (de) Verfahren zur bestimmung von polymerase- aktivität.
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
DE69709804T2 (de) Nachweis von nukleotidsequenzen durch signalamplifizierung
DE69941690D1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
DE69637194D1 (de) In situ-hybridisation zum nachweis von spezifischen nukleinsäuresequenzen in eukaryontischen proben
EP1398378A4 (en) IN NEUROBLASTOM ISOLATED NUCLEIC ACIDS
NO953197L (no) Nukleinsyresonder som er spesifikke for spiroceten Borrelia burgdorferi
KR960037841A (ko) 결핵 진단용 탐침