KR960037841A - 결핵 진단용 탐침 - Google Patents
결핵 진단용 탐침 Download PDFInfo
- Publication number
- KR960037841A KR960037841A KR1019950007771A KR19950007771A KR960037841A KR 960037841 A KR960037841 A KR 960037841A KR 1019950007771 A KR1019950007771 A KR 1019950007771A KR 19950007771 A KR19950007771 A KR 19950007771A KR 960037841 A KR960037841 A KR 960037841A
- Authority
- KR
- South Korea
- Prior art keywords
- oligonucleotide
- diagnostic probe
- tuberculosis
- methionyl
- chain reaction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/0101—Methionine-tRNA ligase (6.1.1.10)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 하기의 염기 서열을 지닌 올리고뉴클레오타이드로 구성된 결핵균 진단용 탐침에 관한 것이다.
DTBF(센스프라이며) : 5'-GCCTACGAATACATCGCCACC-3'
DTBB(안티센스프라이머) : 5'-GGAGACGAAGCTGATCACTTC-3'
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 IS6110 결합 프라이머와 본 발명의 프라이머간의 PCR반응을 비교한 전기영동 사진이다. 제2도는 본 발명의 프라이머의 결핵균 특이성 시험시 나타난 전기영동 사진이다.
Claims (3)
- 하기의 염기서열을 지는 올리고뉴클레오타이드로 구성된 결합균 진단용 탐침에 관한 것이다.DTBF(센스프라이며) : 5'-GCCTACGAATACATCGCCACC-3'DTBB(안티센스프라이머) : 5'-GGAGACGAAGCTGATCACTTC-3'
- 제1항의 올리고뉴클레오타이드가 결핵균내의 메티오닐-tRNA 신쎄타제와 중합 효소연쇄반응(PCR)의 수행 여부를 전기영동등의 방법으로 측정하여 결핵감염여부를 진단하는 방법.
- 제1항의 올리고뉴클레오타이드와 메티오닐-tRNA 신쎄타제와 효소중합연쇄반응을 측정할 수 있는 지시제등을 첨가하여 제조된 결핵 진단용 키드.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950007771A KR0135456B1 (ko) | 1995-04-04 | 1995-04-04 | 결핵 진단용 탐침 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950007771A KR0135456B1 (ko) | 1995-04-04 | 1995-04-04 | 결핵 진단용 탐침 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR960037841A true KR960037841A (ko) | 1996-11-19 |
KR0135456B1 KR0135456B1 (ko) | 1998-04-23 |
Family
ID=19411482
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019950007771A KR0135456B1 (ko) | 1995-04-04 | 1995-04-04 | 결핵 진단용 탐침 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR0135456B1 (ko) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20020063122A (ko) * | 2001-01-26 | 2002-08-01 | (주)네오딘 | 결핵균 검출용 키트 및 그 검출방법 |
-
1995
- 1995-04-04 KR KR1019950007771A patent/KR0135456B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR0135456B1 (ko) | 1998-04-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR930010192A (ko) | 에이치엘에이(hla) 제i형 디엠에이(dna) 타이핑 방법 및 시약 | |
DE69421514T2 (de) | Reagens und verfahren für den nachweis einer nukleotidischen sequenz mit signalamplifizierung | |
FI950487A (fi) | Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi | |
ATE147791T1 (de) | Festphasendiagnose von medizinischen konditionen | |
PL278176A1 (en) | Method of detecting sequences of nucleotides and sets for carrying out diagnostic tests | |
WO1996013612A3 (en) | Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences | |
PT777747E (pt) | Metodo de sequenciacao nucleotidica | |
ES2165402T3 (es) | Procedimiento para la deteccion sensible de acidos nucleicos. | |
AU3360101A (en) | Method for quickly detecting microbial dna/rna, kit therefor and the use of saidmethod | |
ES8605862A1 (es) | Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad | |
EP1746156A4 (en) | PROBE AND PRIMER FOR THE DETECTION OF TUBERCELIC ACIDS AND PROCEDURE FOR THE DETECTION OF TUBERCELIC ACIDS IN HUMANS | |
IN188176B (ko) | ||
WO1991017264A1 (en) | Assay for specific nucleic acid sequences | |
PT887425E (pt) | Ensaio de pcr multiplex para detectar infeccao pelo complexo de mycobacterium avium | |
BR9606602A (pt) | Primer de amplificação oligonucleotídeo e método para ampliação específica comlexa de um ácido | |
WO1996006949A3 (en) | Nucleic acid hybridization assay probes targeted to mycoplasma pneumoniae nucleic acid | |
EP0707075A3 (en) | Species-specific detection of Mycobacterium kansasii | |
GB2366378A (en) | Genetic marker test for lupus | |
KR960705060A (ko) | 미코박테리움 아비움 콤플렉스에 대한 핵산 프로브(Nucleic Acid Probes to Mycobacterium Avium Complex) | |
KR960037841A (ko) | 결핵 진단용 탐침 | |
ATE415491T1 (de) | Oligonucleotide zur markierung von oligonukleotidsonden und proteine | |
KR930019830A (ko) | 사람 아릴아민 n-아세틸 전이효소 유전자 | |
WO1998000565A3 (en) | NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(MYCOPLASMA PNEUMONIAE) | |
EP1705255A3 (en) | Diagnostic method and kit for determining Kell blood group genotype | |
EP1398378A4 (en) | IN NEUROBLASTOM ISOLATED NUCLEIC ACIDS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20070112 Year of fee payment: 10 |
|
LAPS | Lapse due to unpaid annual fee |