DE69924140D1 - Bestimmung der länge von repetitiven nukleinsäuresequenzen durch eine diskontinuierliche verlängerung von startermolekülen - Google Patents

Bestimmung der länge von repetitiven nukleinsäuresequenzen durch eine diskontinuierliche verlängerung von startermolekülen

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Publication number
DE69924140D1
DE69924140D1 DE69924140T DE69924140T DE69924140D1 DE 69924140 D1 DE69924140 D1 DE 69924140D1 DE 69924140 T DE69924140 T DE 69924140T DE 69924140 T DE69924140 T DE 69924140T DE 69924140 D1 DE69924140 D1 DE 69924140D1
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DE
Germany
Prior art keywords
nucleic acid
moleculars
starter
determination
length
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69924140T
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English (en)
Other versions
DE69924140T2 (de
Inventor
J Livak
L Lowe
J Blasband
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
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Applera Corp
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Publication of DE69924140D1 publication Critical patent/DE69924140D1/de
Publication of DE69924140T2 publication Critical patent/DE69924140T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69924140T 1998-12-03 1999-12-01 Bestimmung der länge von repetitiven nukleinsäure-sequenzen durch eine diskontinuierliche primerverlängerung Expired - Lifetime DE69924140T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US205114 1998-12-03
US09/205,114 US6309829B1 (en) 1997-05-27 1998-12-03 Length determination of nucleic acid repeat sequences by discontinuous primer extension
PCT/US1999/028584 WO2000032824A2 (en) 1998-12-03 1999-12-01 Length determination of nucleic acid repeat sequences by discontinuous primer extension

Publications (2)

Publication Number Publication Date
DE69924140D1 true DE69924140D1 (de) 2005-04-14
DE69924140T2 DE69924140T2 (de) 2006-01-19

Family

ID=22760853

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69924140T Expired - Lifetime DE69924140T2 (de) 1998-12-03 1999-12-01 Bestimmung der länge von repetitiven nukleinsäure-sequenzen durch eine diskontinuierliche primerverlängerung

Country Status (8)

Country Link
US (4) US6309829B1 (de)
EP (1) EP1135528B1 (de)
JP (2) JP2002531106A (de)
AT (1) ATE290606T1 (de)
AU (1) AU758964B2 (de)
CA (1) CA2353793A1 (de)
DE (1) DE69924140T2 (de)
WO (1) WO2000032824A2 (de)

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US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
EP1337541B1 (de) 2000-10-06 2007-03-07 The Trustees of Columbia University in the City of New York Massives Parallelverfahren zur Dekodierung von DNA und RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US20040185477A1 (en) * 2003-01-10 2004-09-23 Sention Inc. Methods of detecting differences in genomic sequence representation
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
WO2006034387A1 (en) 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
US20070212695A1 (en) * 2005-01-12 2007-09-13 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
WO2007053719A2 (en) * 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
US7982029B2 (en) 2005-10-31 2011-07-19 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods
US20080038727A1 (en) * 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
EP2484781B1 (de) 2006-08-01 2017-08-23 Applied Biosystems, LLC Nachweis von Analyten und Nukleinsäuren
WO2008069973A2 (en) * 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
EP2940029B1 (de) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
US20100330569A1 (en) * 2009-04-23 2010-12-30 Intelligent Bio-Systems, Inc. Hydroxymethyl Linkers For Labeling Nucleotides
AU2010271196B2 (en) * 2009-07-10 2015-07-16 Perkinelmer Health Sciences, Inc. Detecting multinucleotide repeats
US20140170653A1 (en) 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
EP3181699A1 (de) 2015-12-17 2017-06-21 Oniris Mikro-rnas als prädiktive biomarker von typ-1-diabetes

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US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5075217A (en) 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5189056A (en) 1989-12-19 1993-02-23 University Of North Carolina At Chapel Hill Protection of moist stratified squamous epithelia against damage from noxious luminal agents
IL97222A (en) 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
JPH0697522A (ja) * 1990-11-30 1994-04-08 Internatl Business Mach Corp <Ibm> 超伝導材料の薄膜の製造方法
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
EP1382386A3 (de) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren
AU3816993A (en) 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US5650277A (en) 1992-07-02 1997-07-22 Diagenetics Ltd. Method of determining the presence and quantifying the number of di- and trinucleotide repeats
US5374527A (en) 1993-01-21 1994-12-20 Applied Biosystems, Inc. High resolution DNA sequencing method using low viscosity medium
AU3933393A (en) 1993-03-17 1994-10-11 City Of Hope Nucleic acid sequences having single stranded ends
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
US5695933A (en) 1993-05-28 1997-12-09 Massachusetts Institute Of Technology Direct detection of expanded nucleotide repeats in the human genome
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
FR2718753B1 (fr) 1994-04-15 1996-07-19 Pasteur Institut Procédé de comptage de mono-, di- et trinucléotides répétés dans un génome eucaryote et trousse permettant la mise en Óoeuvre de ce procédé.
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
WO1996036737A1 (en) 1995-05-19 1996-11-21 Ely Michael Rabani Parallel multiplex polynucleotide sequencing
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
EP0832287B1 (de) 1995-06-07 2007-10-10 Solexa, Inc Oligonukleotid-tags zur sortierung und identifizierung
EP2332958B1 (de) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detektion von Unterschieden in der Sequenz von Nukleinsäuren mittels der Ligasedetektionsreaktion und adressierbaren Arrays
DE19714288A1 (de) * 1997-04-07 1998-10-08 Hilti Ag Bohr- und/oder Meisselgerät
US5945284A (en) * 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension

Also Published As

Publication number Publication date
JP2002531106A (ja) 2002-09-24
US6773887B2 (en) 2004-08-10
DE69924140T2 (de) 2006-01-19
AU2037500A (en) 2000-06-19
US7294464B2 (en) 2007-11-13
US20070178519A1 (en) 2007-08-02
EP1135528A2 (de) 2001-09-26
US20040126756A1 (en) 2004-07-01
CA2353793A1 (en) 2000-06-08
AU758964B2 (en) 2003-04-03
ATE290606T1 (de) 2005-03-15
WO2000032824A3 (en) 2001-05-10
EP1135528B1 (de) 2005-03-09
US6309829B1 (en) 2001-10-30
WO2000032824A9 (en) 2001-07-19
JP2010213709A (ja) 2010-09-30
US20050112619A1 (en) 2005-05-26
WO2000032824A2 (en) 2000-06-08
US7414115B2 (en) 2008-08-19

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

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