ATE414096T1 - Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen - Google Patents

Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen

Info

Publication number
ATE414096T1
ATE414096T1 AT02763230T AT02763230T ATE414096T1 AT E414096 T1 ATE414096 T1 AT E414096T1 AT 02763230 T AT02763230 T AT 02763230T AT 02763230 T AT02763230 T AT 02763230T AT E414096 T1 ATE414096 T1 AT E414096T1
Authority
AT
Austria
Prior art keywords
nucleic acid
detecting
uniquely
genedigits
target specific
Prior art date
Application number
AT02763230T
Other languages
English (en)
Inventor
Krassen Dimitrov
Dwayne Dunaway
Original Assignee
Inst Systems Biology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Systems Biology filed Critical Inst Systems Biology
Application granted granted Critical
Publication of ATE414096T1 publication Critical patent/ATE414096T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT02763230T 2001-07-03 2002-07-03 Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen ATE414096T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/898,743 US7473767B2 (en) 2001-07-03 2001-07-03 Methods for detection and quantification of analytes in complex mixtures

Publications (1)

Publication Number Publication Date
ATE414096T1 true ATE414096T1 (de) 2008-11-15

Family

ID=25409980

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02763230T ATE414096T1 (de) 2001-07-03 2002-07-03 Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen

Country Status (9)

Country Link
US (6) US7473767B2 (de)
EP (1) EP1448581B1 (de)
JP (2) JP4343682B2 (de)
AT (1) ATE414096T1 (de)
AU (1) AU2002327202B2 (de)
CA (3) CA2798555C (de)
DE (1) DE60229873D1 (de)
ES (1) ES2316603T3 (de)
WO (1) WO2003003810A2 (de)

Families Citing this family (283)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EA008220B1 (ru) 2001-10-29 2007-04-27 Круселл Холланд Б.В. Композиция, включающая эритропоэтинподобные молекулы, и способы ее применения
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
JP2005523707A (ja) * 2002-04-23 2005-08-11 ユー.エス. ジェノミクス, インコーポレイテッド 2アーム核酸プローブに関する組成物および方法
EP1543155A4 (de) * 2002-07-17 2006-08-16 Us Genomics Inc Verfahren und zusammensetzungen zur analyse von polymeren unter verwendung chimärer tags
US7632548B2 (en) * 2002-08-02 2009-12-15 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
CN100383238C (zh) 2003-05-09 2008-04-23 克鲁塞尔荷兰公司 E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法
AU2004252554B2 (en) 2003-06-23 2012-01-19 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
EP2354248B1 (de) 2003-06-23 2018-01-03 Epigenomics AG Verfahren zur Analyse von Störungen der Proliferation kolorektaler Zellen
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
US7674628B2 (en) * 2003-08-01 2010-03-09 Applied Nanotech Holdings, Inc. Remote identification of explosives and other harmful materials
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20050123974A1 (en) * 2003-11-17 2005-06-09 U.S. Genomics, Inc. Methods and compositions relating to single reactive center reagents
JP4824575B2 (ja) * 2003-12-01 2011-11-30 エピゲノミクス アクチェンゲゼルシャフト 前立腺細胞増殖性疾患の発症に関連する遺伝子発現の分析のための方法および核酸
DE602004031405D1 (de) * 2003-12-11 2011-03-31 Epigenomics Ag Marker zur Prognose der Reaktion auf Therapie bzw. der Überlebensrate von Brustkrebspatienten
EP1561821B1 (de) 2003-12-11 2011-02-16 Epigenomics AG Marker zur Prognose der Reaktion auf Therapie bzw. der Überlebensrate von Brustkrebspatienten
EP1704256A4 (de) * 2004-01-13 2008-01-16 Us Genomics Inc Nachweis und quantifizierung von analyten in lösung unter verwendung von polymeren
US20050169415A1 (en) * 2004-01-30 2005-08-04 Agere Systems Inc. Timing error recovery system
EP2281902A1 (de) 2004-07-18 2011-02-09 Epigenomics AG Epigenetische Verfahren und Nukleinsäuren für den Nachweis von proliferativen Erkrankungen von Mammazellen
US8029985B2 (en) * 2004-09-01 2011-10-04 Vybion, Inc. Amplified bioassay
WO2006034879A2 (en) * 2004-09-30 2006-04-06 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of lung cell proliferative disorders
JP5745202B2 (ja) * 2004-12-02 2015-07-08 エピゲノミックス アクチェンゲゼルシャフト 前立腺細胞増殖障害の予後に関連する遺伝子発現分析のための方法および核酸
US20070009884A1 (en) * 2005-04-11 2007-01-11 Ghc Technologies, Inc. Methods and apparatuses for detecting chemical or biological agents
KR20080011287A (ko) * 2005-04-15 2008-02-01 에피제노믹스 아게 세포 증식 질환을 분석하기 위한 방법 및 핵산
ES2446250T3 (es) 2005-05-02 2014-03-06 University Of Southern California Marcadores de metilación del ADN asociados con el fenotipo metilador de islas CpG (CIMP) en el cáncer colorrectal humano
WO2007039128A1 (en) * 2005-09-21 2007-04-12 Epigenomics Ag Markers for the prediction of outcome of anthracycline treatment
EP1934369A2 (de) 2005-09-29 2008-06-25 Epigenomics AG Verfahren und nukleinsäuren zur analyse der mit gewebeklassifizierung assoziierten genexpression
WO2007039290A2 (en) * 2005-10-03 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of cell proliferative disorders
US7770717B2 (en) * 2005-10-12 2010-08-10 Scanvaegt International A/S Device for transfer of items
US8986926B2 (en) * 2005-12-23 2015-03-24 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
JP5537034B2 (ja) * 2005-12-23 2014-07-02 ナノストリング テクノロジーズ,インコーポレーテッド ナノレポーターならびにその作製方法および使用方法
AU2013203290B2 (en) * 2005-12-23 2016-05-05 Bruker Spatial Biology, Inc. Nanoreporters and methods of manufacturing and use thereof
JP4621595B2 (ja) * 2006-01-11 2011-01-26 株式会社東芝 半導体装置の製造方法
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
AU2007237444B2 (en) 2006-04-17 2013-05-23 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US7964347B2 (en) * 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
US7312029B1 (en) 2006-06-30 2007-12-25 Searete Llc Method of combing an elongated molecule
US20110171637A1 (en) * 2006-07-21 2011-07-14 Reimo Tetzner Methods and nucleic acids for analyses of cellular proliferative disorders
WO2008011620A2 (en) 2006-07-21 2008-01-24 Epigenomics Ag Methods and nucleic acids for analyses of cellular proliferative disorders
EP2069534A4 (de) * 2006-08-02 2010-08-11 California Inst Of Techn Verfahren und systeme zur erkennung und/oder sortierung von zielen
WO2008021290A2 (en) 2006-08-09 2008-02-21 Homestead Clinical Corporation Organ-specific proteins and methods of their use
US20080050724A1 (en) * 2006-08-24 2008-02-28 Microfluidic Systems, Inc. Method of detecting one or more limited copy targets
US7970534B2 (en) 2006-08-24 2011-06-28 Blackbird Technologies, Inc. Mobile unit and system having integrated mapping, communications and tracking
EP3184649A1 (de) 2006-11-24 2017-06-28 Epigenomics AG Verfahren und nukleinsäuren zur analyse der genexpression, die mit der entwicklung von prostatazellproliferationserkrankungen assoziiert ist
EP2126075A2 (de) * 2007-01-16 2009-12-02 Cytocure, Inc. Verfahren zur isolierung und reinigung nukleinsäurebindender biomoleküle und zusammensetzungen damit
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
DK2258871T3 (da) 2007-01-19 2014-08-11 Epigenomics Ag Fremgangsmåder og nukleinsyrer til analyse af celleproliferative lidelser
US7843335B2 (en) 2007-03-13 2010-11-30 Blackbird Technologies, Inc. Mobile asset tracking unit, system and method
WO2008124847A2 (en) * 2007-04-10 2008-10-16 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2009012340A2 (en) * 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
AU2008334901A1 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of lung carcinoma
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
US8445199B2 (en) * 2008-12-31 2013-05-21 Abbott Point Of Care Inc. Method and device for immunoassay using nucleotide conjugates
US20120184447A1 (en) 2009-06-26 2012-07-19 Reinhold Wasserkort Methods and Nucleic Acids for Analysis of Bladder Cell Proliferative Disorders
EP2309005B1 (de) 2009-08-03 2015-03-04 Epigenomics AG Verfahren zur Konservierung von genomischer DNA-Sequenzkomplexität
AU2015261558B2 (en) * 2009-10-13 2017-09-28 Bruker Spatial Biology, Inc. Protein Detection Via Nanoreporters
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
CA2779750C (en) 2009-11-06 2019-03-19 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
DK2556171T3 (en) 2010-04-05 2015-12-14 Prognosys Biosciences Inc Spatially CODED BIOLOGICAL ASSAYS
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2012012141A1 (en) 2010-06-30 2012-01-26 Amgen Inc. Scnn1a/tnfrsf1a fusion proteins in cancer
WO2012040168A2 (en) 2010-09-20 2012-03-29 Advanced Cell Diagnostics, Inc. Biomarkers for differentiating melanoma from benign nevus in the skin
ES2687761T3 (es) 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8538373B2 (en) 2011-05-25 2013-09-17 Blackbird Technologies, Inc. Methods and apparatus for emergency tracking
CA2840149C (en) 2011-07-08 2021-10-26 Epigenomics Ag Methods and nucleic acids for determining the prognosis of a cancer subject
CA3069089A1 (en) 2011-09-22 2013-03-28 Lineage Biosciences, Inc. Compositions and methods for analyzing heterogeneous samples
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP2794928B1 (de) 2011-12-22 2019-02-20 President and Fellows of Harvard College Zusammensetzungen und verfahren zum nachweis von analyten
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CN104603291B (zh) 2012-06-22 2018-04-06 Htg分子诊断有限公司 黑素细胞病变中的分子恶性肿瘤
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
US10174310B2 (en) 2012-08-08 2019-01-08 Roche Sequencing Solutions, Inc. Increasing dynamic range for identifying multiple epitopes in cells
EP2888349B1 (de) 2012-08-24 2022-02-09 Yale University System, vorrichtung und verfahren für multiplex-erkennung mit hohem durchsatz
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014071247A1 (en) 2012-11-02 2014-05-08 Dana-Farber Cancer Institute, Inc. Pyrrol-1 -yl benzoic acid derivates useful as myc inhibitors
WO2014144822A2 (en) 2013-03-15 2014-09-18 Immumetrix, Inc. Methods and compositions for tagging and analyzing samples
US20140287949A1 (en) * 2013-03-15 2014-09-25 Institute For Systems Biology Multiplex assay for members of binding pairs
MY197877A (en) 2013-06-04 2023-07-22 Harvard College Rna-guided transcriptional regulation
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
KR20160039229A (ko) 2013-07-30 2016-04-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 정량적 dna-기반 영상화 및 초해상도 영상화
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
SG11201602291TA (en) 2013-09-30 2016-04-28 Daiichi Sankyo Co Ltd Nucleic acid biomarker and use thereof
CN106232831B (zh) 2013-11-06 2021-02-26 美国卫生和人力服务部 通过表达谱分析对淋巴瘤类型进行亚型分类的方法
US10011811B2 (en) 2013-11-25 2018-07-03 Vericel Corporation Cell seeding device and method
US10240208B2 (en) 2014-03-31 2019-03-26 H. Lee Moffitt Cancer Center And Research Institute, Inc. MicroRNA assay for detection and management of pancreatic cancer precursors
JP2017516501A (ja) 2014-05-30 2017-06-22 ジーンセントリック ダイアグノスティクス, インコーポレイテッド 肺がんのタイピング法
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
ES2968004T3 (es) 2014-07-30 2024-05-06 Harvard College Construcción de bibliotecas de sondas
CA2957538A1 (en) 2014-08-08 2016-02-11 Nanostring Technologies, Inc. Methods for deconvolution of mixed cell populations using gene expression data
JP2017529103A (ja) * 2014-09-22 2017-10-05 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニアThe Regents of the University of California 単分子rna検出
CA2968376C (en) 2014-11-21 2020-06-23 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
WO2016085841A1 (en) 2014-11-24 2016-06-02 Nanostring Technologies, Inc. Methods and apparatuses for gene purification and imaging
DK3227684T3 (da) 2014-12-03 2019-11-11 Isoplexis Corp Analyse af og screening for cellesekretionsprofiler
EP3234602B1 (de) 2014-12-19 2021-08-25 F. Hoffmann-La Roche AG Verfahren zur identifizierung mehrerer epitope in ausgewählten subpopulationen von zellen
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
EP4282977B1 (de) 2015-04-10 2024-06-05 10x Genomics Sweden AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
AU2016297513B2 (en) 2015-07-17 2021-02-25 Board Of Regents, The University Of Texas System Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP3325650B1 (de) 2015-07-17 2024-01-03 Nanostring Technologies, Inc. Simultane quantifizierung der genexpression in einer benutzerdefinierten region eines gewebequerschnitts
WO2017040526A2 (en) 2015-09-01 2017-03-09 Eisai R&D Management Co., Ltd. Splice variants associated with neomorphic sf3b1 mutants
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
US11384399B2 (en) 2015-09-29 2022-07-12 Htg Molecular Diagnostics, Inc. Methods for subtyping diffuse large B-cell lymphoma (DLBCL)
EP3362462B1 (de) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In-situ-detektion von nukleotidvarianten bei proben mit hohem rauschen sowie zugehörige zusammensetzungen und verfahren
EP3371329B1 (de) 2015-11-03 2026-03-25 President and Fellows of Harvard College Verfahren und vorrichtung zur volumetrischen bildgebung einer dreidimensionalen nukleinsäurehaltigen matrix
US11306362B2 (en) 2016-02-08 2022-04-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Gene signature predictive of hepatocellular carcinoma response to transcatheter arterial chemoembolization (TACE)
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
ES2859423T3 (es) 2016-04-20 2021-10-04 Us Health Evaluación del linfoma de células del manto y procedimientos relacionados con el mismo
CA3210120C (en) 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
JP7131773B2 (ja) 2016-04-29 2022-09-06 ボード オブ リージェンツ,ザ ユニバーシティ オブ テキサス システム ホルモン受容体に関連する転写活性の標的尺度
CA3019589A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
EP3458601B1 (de) 2016-05-16 2023-07-05 Nanostring Technologies, Inc. Verfahren zur erkennung von zielnukleinsäuren in einer probe
JP7241352B2 (ja) 2016-05-17 2023-03-17 ジーンセントリック セラピューティクス, インコーポレイテッド 肺扁平上皮癌のサブタイピングのための方法
CA3024747A1 (en) 2016-05-17 2017-11-23 Genecentric Therapeutics, Inc. Methods for subtyping of lung adenocarcinoma
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光in situ配列決定による検出のための核酸配列ライブラリの作製法
EP3510398B1 (de) 2016-09-12 2026-02-25 Isoplexis Corporation System und verfahren zur multiplex-analyse von zellularen und anderen immunotherapeutika
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
EP3538891B1 (de) 2016-11-11 2022-01-05 Isoplexis Corporation Zusammensetzungen und verfahren zur gleichzeitigen genomischen, transkriptomischen und proteomischen analyse von einzelzellen
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法,以及其制造方法
RU2637623C1 (ru) * 2016-12-27 2017-12-05 Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет им. Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГБОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) Способ оценки риска развития онкозаболеваний в поколениях жителей регионов радионуклидного загрязнения
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CA3019250A1 (en) 2017-04-14 2019-10-13 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
WO2018204725A1 (en) 2017-05-03 2018-11-08 H. Lee Moffitt Cancer Center And Research Institute, Inc. Theranostic tools for management of pancreatic cancer and its precursors
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
EP3638814B8 (de) 2017-06-14 2023-06-21 The United States of America, as Represented by The Secretary, Department of Health and Human Services Verfahren zur bestimmung des lymphomtyps
US11739386B2 (en) 2017-07-21 2023-08-29 Genecentric Therapeutics, Inc. Methods for determining response to PARP inhibitors
EP3665199A4 (de) 2017-08-07 2021-08-11 Genecentric Therapeutics, Inc. Verfahren zur subtypisierung von plattenepithelkarzinomen an kopf und hals
EP4589017A3 (de) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna-template-ligation
JP2021500921A (ja) 2017-10-16 2021-01-14 アンテローム Fimh遮断剤の治療効率を評価するための新規ツール
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
WO2019157445A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
US12195805B2 (en) 2018-02-13 2025-01-14 Genecentric Therapeutics, Inc. Methods for subtyping of bladder cancer
AU2019220440A1 (en) 2018-02-16 2020-09-03 Arc-Net Centre For Applied Research On Cancer Università Degli Studi Di Verona Patient classification and prognostic method
CA3093846A1 (en) 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213478A1 (en) 2018-05-04 2019-11-07 Nanostring Technologies, Inc. Gene expression assay for measurement of dna mismatch repair deficiency
EP3794146B1 (de) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Verfahren zur erkennung einer zuvor bestimmten nukleotidesequenz
US20210363590A1 (en) 2018-05-21 2021-11-25 Nanostring Technologies, Inc. Molecular gene signatures and methods of using same
GB201810190D0 (en) 2018-06-21 2018-08-08 Cancer Research Tech Ltd Prognostic and treatment response predictive method
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
CA3115922A1 (en) 2018-10-09 2020-04-16 Genecentric Therapeutics, Inc. Detecting cancer cell of origin
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
EP3874057A4 (de) * 2018-11-01 2022-08-17 Advanced Cell Diagnostics, Inc. Verfahren zum digitalen in-situ-multiplexen von nukleinsäuren
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
WO2020123311A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
CN113366116A (zh) 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统
EP3894552B1 (de) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
JP7344786B2 (ja) 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のdna配列を同定する方法
JP2023507809A (ja) * 2019-12-19 2023-02-27 アコヤ・バイオサイエンシズ・インコーポレイテッド Rna検出
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
DK3891300T3 (da) 2019-12-23 2023-05-22 10X Genomics Inc Fremgangsmåder til rumlig analyse ved anvendelse af rna-template-ligering
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4090763B1 (de) 2020-01-13 2024-12-04 Becton Dickinson and Company Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
JP2023514749A (ja) 2020-02-21 2023-04-07 10エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4111168A1 (de) 2020-02-25 2023-01-04 Becton Dickinson and Company Bispezifische sonden zur ermöglichung der verwendung von einzelzellenproben als einzelfarbkompensationssteuerung
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
ES3014967T3 (en) 2020-04-22 2025-04-28 10X Genomics Inc Methods for spatial analysis using targeted rna depletion
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
EP4153776B1 (de) 2020-05-22 2025-03-05 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
EP4168578B1 (de) 2020-06-18 2024-08-07 Bruker Spatial Biology, Inc. Zusammensetzungen und verfahren zur in-situ-einzelzellenanalyse unter verwendung von enzymatischer nukleinsäureverlängerung
EP4450639B1 (de) 2020-06-25 2025-10-15 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
GB2597332A (en) 2020-07-20 2022-01-26 Institute Of Cancer Res Prognostic and treatment response predictive method
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
GB202015200D0 (en) 2020-09-25 2020-11-11 Institute Of Cancer Res Royal Cancer Hospital The Prognostic and treatment response predictive method
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
KR20230104204A (ko) 2020-11-04 2023-07-07 에자이 알앤드디 매니지먼트 가부시키가이샤 골수이형성 증후군(mds)에 대한 바이오마커 및 이를 사용하는 방법
EP4240872A1 (de) * 2020-11-06 2023-09-13 Waters Technologies Corporation Verfahren und zusammensetzungen zur nukleinsäureanalyse
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
EP4263859A1 (de) 2020-12-15 2023-10-25 Becton, Dickinson and Company Einzelzellensekretomanalyse
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (de) 2021-01-26 2024-10-23 10X Genomics, Inc. Nukleinsäureanalogsonden zur in-situ-analyse
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (de) 2021-06-03 2025-10-22 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
EP4355907A1 (de) 2021-06-17 2024-04-24 Nanostring Technologies, Inc. Zusammensetzungen und verfahren zur in-situ-einzelzellenanalyse unter verwendung von enzymatischer nukleinsäureverlängerung
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
ES2988400T3 (es) 2021-07-30 2024-11-20 10X Genomics Inc Métodos y composiciones para sincronizar reacciones in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
EP4446426B1 (de) 2021-08-16 2026-03-04 10X Genomics, Inc. Sonden mit einem geteilten strichcodebereich und verfahren zur verwendung
EP4137585A1 (de) 2021-08-20 2023-02-22 Institut D'Investigacions Biomèdiques August Pi I Sunyer - IDIBAPS Krebsinformationsbiomarkersignatur
EP4196605B1 (de) 2021-09-01 2024-12-04 10X Genomics, Inc. Verfahren zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
GB202116745D0 (en) 2021-11-19 2022-01-05 Institute Of Cancer Res Royal Cancer Hospital Prognostic and treatment response predictive method
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023131866A1 (en) 2022-01-05 2023-07-13 Eisai R&D Management Co., Ltd. Biomarkers for myelodysplastic syndrome (mds) and methods of using the same
EP4466376B1 (de) 2022-01-21 2026-03-04 10X Genomics, Inc. Mehrere auslesesignale zur analyse einer probe
EP4490737A1 (de) 2022-03-08 2025-01-15 10X Genomics, Inc. In-situ-codedesignverfahren zur minimierung von optischem crowding
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
WO2024079279A1 (en) 2022-10-12 2024-04-18 F. Hoffmann-La Roche Ag Disease characterisation
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
EP4482979B1 (de) 2022-11-09 2025-10-01 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
EP4573210A1 (de) 2022-11-16 2025-06-25 10X Genomics, Inc. Verfahren und zusammensetzungen zur beurteilung der leistung von in-situ-tests
EP4634404A1 (de) 2022-12-16 2025-10-22 10X Genomics, Inc. Verfahren und zusammensetzungen zur beurteilung der leistung
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
GB202312984D0 (en) 2023-08-25 2023-10-11 Cancer Research Tech Ltd Method of predicting prognosis and treatment response
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
EP4653542A1 (de) * 2024-05-21 2025-11-26 Leica Microsystems CMS GmbH Etikett, marker und verfahren zur analyse einer biologischen probe
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2026030369A1 (en) 2024-07-31 2026-02-05 10X Genomics, Inc. Methods and compositions for in situ analyte detection

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2825529C2 (de) * 1978-06-10 1980-11-13 Manfred Kammer Stückförmiges Reinigungsmittel, insbesondere Seife
GB8405437D0 (en) 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
US4824775A (en) * 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US5175270A (en) 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US4824447A (en) * 1986-12-30 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Enhanced oil recovery system
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5763162A (en) * 1990-03-14 1998-06-09 The Regents Of University Of California Multichromophore fluorescent DNA intercalation complexes
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5293050A (en) 1993-03-25 1994-03-08 International Business Machines Corporation Semiconductor quantum dot light emitting/detecting devices
US5543838A (en) 1993-08-31 1996-08-06 Xerox Corporation Signal multiplexing system for an image sensor array
US5679519A (en) * 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
WO1997007245A1 (en) 1995-08-14 1997-02-27 Ely Michael Rabani Methods and devices for parallel multiplex polynucleotide sequencing
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
AU7398996A (en) 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6277583B1 (en) * 1996-02-07 2001-08-21 Conjuchem, Inc. Affinity labeling libraries and applications thereof
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7144119B2 (en) 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1818417B1 (de) * 1996-07-29 2014-02-12 Nanosphere, Inc. Nanopartikel mit angehängten Oligonukleotiden und ihre Verwendungen
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
US6180415B1 (en) 1997-02-20 2001-01-30 The Regents Of The University Of California Plasmon resonant particles, methods and apparatus
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
JP2001514859A (ja) 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US6632526B1 (en) * 1997-10-14 2003-10-14 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
ATE239801T1 (de) * 1998-01-22 2003-05-15 Luminex Corp Mikropartikel mit multiplen fluoreszenz-signalen
ATE333499T1 (de) 1998-03-18 2006-08-15 Quark Biotech Inc Selektion/subtraktionsansatz zur genidentifizierung
AU3555599A (en) * 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
JP2002517183A (ja) 1998-06-02 2002-06-18 イェール ユニバーシティ マルチパラメトリック蛍光in−situハイブリッド形成法
DE69940430D1 (de) 1998-06-24 2009-04-02 Illumina Inc Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
EP1271154A3 (de) 1998-09-18 2005-08-17 Massachusetts Institute Of Technology Biologische Verwendung von Halbleiter-Nanokristallen
US6261779B1 (en) * 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
EP1006199A1 (de) 1998-12-03 2000-06-07 Kreatech Biotechnology B.V. Verfahren zur Herstellung von Verknüpfungen an ausgewählten Positionen von Nukleinsäuresträngen und deren Verwendung
JP4014742B2 (ja) 1998-12-04 2007-11-28 キヤノンファインテック株式会社 シート処理装置及び画像形成装置
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
AU780119B2 (en) 1999-01-06 2005-03-03 Cornell Research Foundation Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CA2366289A1 (en) 1999-03-25 2000-09-28 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
JP2002542793A (ja) 1999-04-22 2002-12-17 ザ アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ 多蛍光fishによる遺伝子発現パターンのアッセイ法
AU4701200A (en) 1999-05-07 2000-11-21 Quantum Dot Corporation A method of detecting an analyte using semiconductor nanocrystals
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001000875A2 (en) 1999-06-25 2001-01-04 Motorola, Inc. Novel methods and products for arrayed microsphere analysis
AU5522600A (en) * 1999-06-29 2001-01-22 Dako A/S Detection using dendrimers bearing labels and probes
EP1212599A2 (de) * 1999-08-30 2002-06-12 Illumina, Inc. Verfahren zur verbesserung des signals einer sensorarray
EP1294930B1 (de) 2000-01-13 2011-03-30 Nanosphere, Inc. Nanopartikel an denen oligonukleotide gebunden sind und deren verwendungen
WO2003074738A1 (en) 2000-01-18 2003-09-12 Quantom Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
US20020028457A1 (en) 2000-02-16 2002-03-07 Quantum Dot Corporation Single target counting assays using semiconductor nanocrystals
CN1416365A (zh) 2000-02-22 2003-05-07 基因谱公司 微阵列制造技术及设备
US20030186426A1 (en) 2000-03-15 2003-10-02 The Regents Of The University Of California Multichannel flow cell for interacting single optically trapped, DNA molecules with different chemical species
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
WO2002009836A2 (en) 2000-08-01 2002-02-07 Surromed, Inc. Methods for solid phase nanoextraction and desorption
WO2002022889A2 (en) 2000-09-11 2002-03-21 President And Fellows Of Harvard College Direct haplotyping using carbon nanotube probes
US6777244B2 (en) 2000-12-06 2004-08-17 Hrl Laboratories, Llc Compact sensor using microcavity structures
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
ATE431853T1 (de) 2001-03-28 2009-06-15 Nanosphere Inc Auf oligonukleotid-modifizierten partikeln basierende bio-strichcodes
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures

Also Published As

Publication number Publication date
US7919237B2 (en) 2011-04-05
US20030013091A1 (en) 2003-01-16
JP2009178171A (ja) 2009-08-13
CA2798555C (en) 2015-09-01
CA2452712C (en) 2013-02-12
US9920380B2 (en) 2018-03-20
CA2452712A1 (en) 2003-01-16
US8148512B2 (en) 2012-04-03
CA2798555A1 (en) 2003-01-16
US20110207623A1 (en) 2011-08-25
AU2002327202B2 (en) 2008-11-20
DE60229873D1 (de) 2008-12-24
EP1448581A4 (de) 2006-11-22
ES2316603T3 (es) 2009-04-16
US8492094B2 (en) 2013-07-23
US20140162251A1 (en) 2014-06-12
CA2893908C (en) 2019-05-14
WO2003003810A3 (en) 2003-11-27
JP2004537301A (ja) 2004-12-16
WO2003003810A2 (en) 2003-01-16
US20070166708A1 (en) 2007-07-19
EP1448581B1 (de) 2008-11-12
CA2893908A1 (en) 2003-01-16
US20110003715A1 (en) 2011-01-06
US7473767B2 (en) 2009-01-06
JP4343682B2 (ja) 2009-10-14
US20090220978A1 (en) 2009-09-03
EP1448581A2 (de) 2004-08-25

Similar Documents

Publication Publication Date Title
ATE414096T1 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
Chen et al. Enzyme-free strip biosensor for amplified detection of Pb 2+ based on a catalytic DNA circuit
AU658962B2 (en) Rapid assays for amplification products
WO2002008414A1 (en) Novel nucleic acid probes and method of assaying nucleic acid by using the same
DE69535240D1 (de) Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
ATE384803T1 (de) Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens
DE69522176D1 (de) Verfahren zur Dämpfung der Fluoreszenz von Fluoreszenzmarkierten Oligonukleotiden in Lösung
CA2330673A1 (en) Method of determining the nucleotide sequence of oligonucleotides and dna molecules
ATE305520T1 (de) Verfahren zur relativen quantifizierung der methylierung von cytosin basen in dna-proben
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
ES543660A0 (es) Un metodo para determinar una secuencia particular de poli- nucleotidos en un medio de ensayo que contiene acidos nu- cleicos monocatenarios
WO2004045365A3 (en) Apparatus and methods for detecting a microbe in a sample
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
DE69839235D1 (de) Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung
EP1321520A4 (de) Neuartige nukleinsäuresonde und neuartiges verfahren zur analyse von nukleinsäuren unter verwendung derselben
DE59913055D1 (de) DNA-Nachweis über einen Strangreassoziationskomplex
HUP0303377A2 (hu) Eljárás nukleinsav-molekulák kimutatására
WO2008068636A3 (en) Blocking agents comprising non-natural nucleic acids and detection methods using such blocking agents
ATE90971T1 (de) Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer.
ATE394417T1 (de) Sonde zum nachweis von nukleinsäuren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
DE502005010036D1 (de) Verfahren zur geno- und pathotypisierung von pseudomonas aeruginosa
EP1323834A3 (de) Detektionsmethode für Zielmoleküle, Reagenz sowie Verfahren zur Detektion von SNPs in DNA Nukleotidsequenzen
WO2005001124A3 (en) Methods of nucleic acid analysis
ATE40409T1 (de) Photoimmunologisches testverfahren fuer dns und rns.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties