DE60016947D1 - Template abhängige ligierung mit pna-dna chimerischen sonden - Google Patents

Template abhängige ligierung mit pna-dna chimerischen sonden

Info

Publication number
DE60016947D1
DE60016947D1 DE60016947T DE60016947T DE60016947D1 DE 60016947 D1 DE60016947 D1 DE 60016947D1 DE 60016947 T DE60016947 T DE 60016947T DE 60016947 T DE60016947 T DE 60016947T DE 60016947 D1 DE60016947 D1 DE 60016947D1
Authority
DE
Germany
Prior art keywords
pna
ligation
gluing
dna chimeric
chimeric probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60016947T
Other languages
English (en)
Other versions
DE60016947T2 (de
Inventor
Michael Egholm
Caifu Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of DE60016947D1 publication Critical patent/DE60016947D1/de
Publication of DE60016947T2 publication Critical patent/DE60016947T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60016947T 1999-10-08 2000-10-06 Template Abhängige Ligierung mit PNA-DNA chimerischen Sonden Expired - Lifetime DE60016947T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US416003 1999-10-08
US09/416,003 US6297016B1 (en) 1999-10-08 1999-10-08 Template-dependent ligation with PNA-DNA chimeric probes
PCT/US2000/027730 WO2001027326A2 (en) 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes

Publications (2)

Publication Number Publication Date
DE60016947D1 true DE60016947D1 (de) 2005-01-27
DE60016947T2 DE60016947T2 (de) 2005-12-15

Family

ID=23648121

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60016947T Expired - Lifetime DE60016947T2 (de) 1999-10-08 2000-10-06 Template Abhängige Ligierung mit PNA-DNA chimerischen Sonden

Country Status (8)

Country Link
US (2) US6297016B1 (de)
EP (1) EP1220953B1 (de)
JP (2) JP3789817B2 (de)
AT (1) ATE285479T1 (de)
AU (1) AU768240B2 (de)
CA (1) CA2353801A1 (de)
DE (1) DE60016947T2 (de)
WO (1) WO2001027326A2 (de)

Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6368801B1 (en) * 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
JP3596868B2 (ja) * 2000-11-24 2004-12-02 キヤノン株式会社 末端標識プローブアレイのプローブの量の評価方法、及び、該標識プローブアレイを用いた標的物質量の評価方法
AU2002230901B2 (en) * 2000-12-14 2006-09-07 Gen-Probe Incorporated Method and kit for enhancing the association rates of polynucleotides
WO2002072865A2 (en) * 2001-03-09 2002-09-19 Boston Probes, Inc. Methods, kits and compositions of combination oligomers
EP1368496B1 (de) * 2001-03-15 2010-09-22 Genzyme Corporation Verfahren zum Nachweis einer Mutation in einer Nukleinsäure
AU2002360361A1 (en) * 2001-11-09 2003-06-10 Biomicroarrays, Inc. High surface area substrates for microarrays and methods to make same
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US6686162B2 (en) 2001-12-04 2004-02-03 Quest Diagnostics Investments, Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US20030215842A1 (en) * 2002-01-30 2003-11-20 Epigenomics Ag Method for the analysis of cytosine methylation patterns
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
JP4691014B2 (ja) * 2003-02-26 2011-06-01 カリダ ゲノミクス,インコーポレーテッド ハイブリダイゼーションによるランダムアレイdna分析
US20040259128A1 (en) * 2003-03-24 2004-12-23 Glenn Kawasaki Methods and compositions for detecting the presence of target nucleic acids in a sample
US20040203005A1 (en) * 2003-04-14 2004-10-14 White Wanda L. B. Dual hybridization of complex nucleic acid samples for sequencing and single-nucleotide polymorphism identification
US7354716B2 (en) * 2003-08-11 2008-04-08 Georgia State University Research Foundation, Inc. RNA detection and quantitation
US20050153300A1 (en) * 2004-01-09 2005-07-14 Quest Diagnostics Incorporated Methods and compositions for the detection of mucolipidosis IV mutations
EP2208797A3 (de) 2004-03-01 2010-11-24 Applied Biosystems, LLC Verfahren, Zusammensetzungen und Kits für Polynukleotidamplifikation
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
US7879569B2 (en) 2004-11-09 2011-02-01 The Trustees Of The University Of Pennsylvania Methods for diagnosis of myelodysplastic syndromes (MDS)
US7700287B2 (en) * 2005-01-28 2010-04-20 Life Technologies Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template
ATE454476T1 (de) * 2005-04-14 2010-01-15 Applied Biosystems Llc 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden
EP3492602A1 (de) * 2005-06-15 2019-06-05 Complete Genomics, Inc. Einzelne molekülarrays für genetische und chemische analyse
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
US7892737B2 (en) * 2005-06-30 2011-02-22 Life Technologies Corporation Compositions, kits and methods pertaining to stability modulation of PNA oligomer/nucleic acid complexes
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US7951572B2 (en) * 2006-02-27 2011-05-31 Korea Advanced Institute Of Science And Technology Construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof
US8859752B2 (en) 2006-04-18 2014-10-14 The Trustees Of The University Of Pennsylvania SIRNA-based therapy of Fibrodyplasia Ossificans Progressiva (FOP)
WO2007123896A2 (en) 2006-04-18 2007-11-01 The Trustees Of The University Of Pennsylvania Mutated acvr1 for diagnosis and treatment of fibrodyplasia ossificans progressiva (fop)
WO2007133703A2 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
WO2007140417A2 (en) 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
AU2007257162A1 (en) 2006-06-05 2007-12-13 Cancer Care Ontario Assessment of risk for colorectal cancer
EP2029726A4 (de) * 2006-06-09 2010-07-28 Univ Miami Beurteilung der zellenzusammensetzung und fraktionelle durchführbarkeit und verwendungen davon
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
US20080131880A1 (en) * 2006-11-22 2008-06-05 Bortolin Laura T PNA-DNA oligomers and methods of use thereof
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
EP2118298B1 (de) 2007-02-08 2013-01-09 Sequenom, Inc. Tests auf nukleinsäurebasis für rhd-typisierung
EP2243834A1 (de) 2007-03-05 2010-10-27 Cancer Care Ontario Abschätzung des Kolorektalkarzinomrisikos
CA2680588A1 (en) 2007-03-26 2008-10-02 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8008010B1 (en) 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
US20090061424A1 (en) * 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
WO2009061840A1 (en) 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
KR20090081260A (ko) * 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US20090270273A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3722810A3 (de) 2009-02-11 2021-01-13 Caris MPI, Inc. Molekulare profilierung von tumoren
ES2469092T3 (es) 2009-04-01 2014-06-17 Dxterity Diagnostics Incorporated Amplificación de sondas dependiente de ligamiento químico (CLPA)
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
US8728725B2 (en) * 2009-07-06 2014-05-20 Trilink Biotechnologies Chemically modified ligase cofactors, donors and acceptors
WO2011056688A2 (en) 2009-10-27 2011-05-12 Caris Life Sciences, Inc. Molecular profiling for personalized medicine
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
US9777332B2 (en) 2011-03-31 2017-10-03 St. Jude Children's Research Hospital Methods and compositions for identifying minimal residual disease in acute lymphoblastic leukemia
CN106434871B (zh) 2011-05-17 2020-08-18 德克斯特里蒂诊断公司 用于检测目标核酸的方法与组合物
EP4155401A1 (de) 2012-03-02 2023-03-29 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
KR101974577B1 (ko) * 2012-05-21 2019-05-02 삼성전자주식회사 나노입자 제작용 주형 및 이를 이용한 나노입자의 제조 방법
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
EP2929050A1 (de) 2012-12-10 2015-10-14 AdvanDx, Inc. Verwendung von sonden zur massenspektrometrischen identifizierung von mikroorganismen oder zellen und relevanten erkrankungen von interesse
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
US9289502B2 (en) 2013-03-08 2016-03-22 Emerald Therapeutics, Inc. Preparation of oligo conjugates
EP2971100A1 (de) 2013-03-13 2016-01-20 Sequenom, Inc. Primer zur analyse der dna-methylierung
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2015274660B2 (en) 2014-06-10 2020-07-16 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
EP3299472A1 (de) * 2016-09-27 2018-03-28 Deutsches Krebsforschungszentrum Stiftung des Öffentlichen Rechts Verfahren zur markierung von oligonukleotidsonden
CA3038178A1 (en) 2016-09-30 2018-04-05 The Governing Council Of The University Of Toronto System for identifying and targeting individual cells within a heterogeneous population for selective extraction of cellular content
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
WO2019202536A1 (en) 2018-04-18 2019-10-24 St. Jude Children's Research Hospital Genotyping assays to identify mutations in xaf1
WO2020113237A1 (en) 2018-11-30 2020-06-04 Caris Mpi, Inc. Next-generation molecular profiling
EP4005634A4 (de) * 2019-07-24 2023-11-08 Tohoku University Chimäres molekül, pharmazeutische zusammensetzung, verfahren zur spaltung von zielnukleinsäure und kit zur spaltung von zielnukleinsäuren oder zur diagnose
WO2021112918A1 (en) 2019-12-02 2021-06-10 Caris Mpi, Inc. Pan-cancer platinum response predictor
WO2023069429A1 (en) * 2021-10-19 2023-04-27 Oncogenuity, Inc. Peptide nucleic acid compositions and methods of use for gene editing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995008556A1 (en) 1993-09-21 1995-03-30 Amersham International Plc Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
DE19637339A1 (de) 1996-09-13 1998-03-19 Hoechst Ag Verfahren zur Amplifikation von Nukleinsäuren
US6361942B1 (en) * 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes

Also Published As

Publication number Publication date
WO2001027326A3 (en) 2002-05-10
DE60016947T2 (de) 2005-12-15
US20020177133A1 (en) 2002-11-28
AU7871200A (en) 2001-04-23
CA2353801A1 (en) 2001-04-19
AU768240B2 (en) 2003-12-04
WO2001027326A2 (en) 2001-04-19
EP1220953A2 (de) 2002-07-10
ATE285479T1 (de) 2005-01-15
JP2006055177A (ja) 2006-03-02
US6297016B1 (en) 2001-10-02
JP3789817B2 (ja) 2006-06-28
US6469151B1 (en) 2002-10-22
EP1220953B1 (de) 2004-12-22
JP2003511059A (ja) 2003-03-25

Similar Documents

Publication Publication Date Title
DE60016947D1 (de) Template abhängige ligierung mit pna-dna chimerischen sonden
WO2001012852A3 (en) Polymerase extension at 3' terminus of pna-dna chimera
US20110014617A1 (en) Methods and Systems for Detecting Nucleic Acids
EP2366803A3 (de) Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
BR0214683A (pt) Molécula de nucleotìdeo ou de nucleosìdeo, métodos de rotular uma molécula de ácido nucléico e para determinar a sequência de um polinucleotìdeo alvo de filamento único, e, kit
CA2039517A1 (en) Dna probe signal amplification
EP1442142A4 (de) Nukleinsäuresonden und verfahren zum nachweis und zur quantifizierung von nukleinsäureanalyten
JPH08211050A (ja) 特定核酸配列の検出方法
CA2308599A1 (en) Dna polymorphism identity determination using flow cytometry
DK1423528T3 (da) Fremgangsmåde til bestemmelse af methyleringsgraden af bestemte cytosiner i genomisk DNA i sekvenskonteksten 5-CPG-3
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
JP2005521409A (ja) 単一プライマー等温核酸増幅−増強型被分析物検出および定量
JP2005502346A (ja) 核酸配列の非特異的ハイブリダイゼーションをブロックするための方法
ATE288963T1 (de) Vergleichende fluoreszenz-hybridisierung auf oligonukleotid mikroarrays
NO20004962L (no) Fremgangsmåte ved påvisning av nukleinsyrer
CN100354298C (zh) 使用单核苷酸多态性组分析受损样品的方法和组合物
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
EP3633049A1 (de) Neuartige fluoreszenzlöschungssonde zur messung von nukleinsäure
WO2010113088A1 (en) Methylation ligation-dependent macroarray (mlm)
ATE287969T1 (de) Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung
WO2003052115A3 (en) Post-synthesis labeling of nucleic acids and uses thereof
FI953897A (fi) Nukleiinihappokeottimia, jotka on kemiallisesti modifioituja asemassa 5'-OH ja/tai 3' -OH yhden tai useamman ei-radioaktiivisesti merkatun aineen viemiseksi näihin asemiin ja menetelmä näiden valmistamiseksi
US8808993B2 (en) Methods and kits to detect new H1N1 “swine flu” variants
EP1619258A4 (de) Verfahren zum nachweis oder zur quantitativen bestimmung der mitochondrialen dna-variation 3243 und kit dafür
KR20100085923A (ko) 핵산프로브 및 프로브폴리머의 형성방법

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN