BR0112400A - Ensaio de hibridização triplex mediado por cátions - Google Patents
Ensaio de hibridização triplex mediado por cátionsInfo
- Publication number
- BR0112400A BR0112400A BR0112400-5A BR0112400A BR0112400A BR 0112400 A BR0112400 A BR 0112400A BR 0112400 A BR0112400 A BR 0112400A BR 0112400 A BR0112400 A BR 0112400A
- Authority
- BR
- Brazil
- Prior art keywords
- probe
- target
- nucleic acid
- cation
- triplex
- Prior art date
Links
- 238000003556 assay Methods 0.000 title abstract 3
- 238000009396 hybridization Methods 0.000 title abstract 2
- 150000001768 cations Chemical class 0.000 title 1
- 230000001404 mediated effect Effects 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 238000009830 intercalation Methods 0.000 abstract 1
- 208000014451 palmoplantar keratoderma and congenital alopecia 2 Diseases 0.000 abstract 1
- 239000012429 reaction media Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
"ENSAIO DE HIBRIDIZAçãO TRIPLEX MEDIADO POR CáTIONS". Os complexos triplexes contem uma sonda de fita única ligada a um alvo de ácido nucléico de fita dupla onde a sonda contém um ácido nucléico heteropolimérico ou um análogo do ácido nucleico heteropolimérico. Todos os triplexes base do complexo são membros selecionados a partir do grupo que consiste de A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G, e C-G-C. Um ensaio facilitado por cátions inclui detectar a presença de tais complexos triplexes para determinar o grau de complementaridade entre a sonda e a sequência alvo. Preferencialmente o ensaio detecta uma alteração na intensidade fluorescente de uma marcação como função de afinidade de ligação entre a sonda e o alvo. A marcação pode ser covalentemente ligada à sonda ou ao alvo, ou pode ser um fluoforo de intercalação no meio de reação.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/613,263 US6420115B1 (en) | 1999-12-21 | 2000-07-10 | Cation mediated triplex hybridization assay |
PCT/IB2001/001538 WO2002004655A2 (en) | 2000-07-10 | 2001-07-09 | Cation mediated triplex hybridization assay |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0112400A true BR0112400A (pt) | 2004-07-20 |
Family
ID=24456561
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0112400-5A BR0112400A (pt) | 2000-07-10 | 2001-07-09 | Ensaio de hibridização triplex mediado por cátions |
Country Status (13)
Country | Link |
---|---|
US (2) | US6420115B1 (pt) |
EP (1) | EP1307591A4 (pt) |
JP (1) | JP2004511218A (pt) |
KR (1) | KR100488826B1 (pt) |
CN (1) | CN1192117C (pt) |
AU (2) | AU8000701A (pt) |
BR (1) | BR0112400A (pt) |
CA (1) | CA2415493C (pt) |
IL (2) | IL153548A0 (pt) |
IN (1) | IN2004CH01656A (pt) |
MX (1) | MXPA03000340A (pt) |
RU (1) | RU2273643C2 (pt) |
WO (1) | WO2002004655A2 (pt) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
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US6656692B2 (en) * | 1999-12-21 | 2003-12-02 | Ingeneus Corporation | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
US6900300B1 (en) * | 2000-09-19 | 2005-05-31 | Ingeneus Corporation | Quadruplex DNA and duplex probe systems |
US6911536B1 (en) * | 1999-12-21 | 2005-06-28 | Ingeneus Corporation | Triplex and quadruplex catalytic hybridization |
US7309569B2 (en) | 1999-12-21 | 2007-12-18 | Ingeneus, Inc. | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
US20030181412A1 (en) * | 1999-12-21 | 2003-09-25 | Ingeneus Corporation | Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses |
US6927027B2 (en) * | 1999-12-21 | 2005-08-09 | Ingeneus Corporation | Nucleic acid multiplex formation |
US20030170659A1 (en) * | 2000-01-24 | 2003-09-11 | Ingeneus Corporation | Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding |
US7220541B2 (en) | 2000-01-24 | 2007-05-22 | Ingeneus, Inc. | Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions |
WO2004010584A2 (en) * | 2002-07-24 | 2004-01-29 | Congruence Llc. | Code for object identification |
CA2494478A1 (en) * | 2002-08-01 | 2004-02-12 | Sensor Technologies Llc | Fluorescence correlation spectroscopy instrument |
US20040142329A1 (en) * | 2003-01-17 | 2004-07-22 | Ingeneus Corporation | Probe conjugation to increase multiplex binding motif preference |
US20040180345A1 (en) * | 2003-03-14 | 2004-09-16 | Ingeneus Corporation | Pre-incubation method to improve signal/noise ratio of nucleic acid assays |
GB0310270D0 (en) * | 2003-05-03 | 2003-06-11 | Univ Edinburgh | Biomolecular devices |
WO2006033088A2 (en) * | 2004-09-24 | 2006-03-30 | Ingeneus Inc. | Genomic assay |
GB0424953D0 (en) | 2004-11-11 | 2004-12-15 | Plant Bioscience Ltd | Assay |
WO2006081426A2 (en) * | 2005-01-28 | 2006-08-03 | Applera Corporation | Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template |
US20090018028A1 (en) * | 2005-05-12 | 2009-01-15 | Stuart Lindsay | Self-Assembled Nucleic Acid Nanoarrays and Uses Therefor |
WO2009037511A1 (en) * | 2007-09-21 | 2009-03-26 | Astrazeneca Ab | Assay for dna supercoiling/relaxation |
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US5176996A (en) * | 1988-12-20 | 1993-01-05 | Baylor College Of Medicine | Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use |
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-
2000
- 2000-07-10 US US09/613,263 patent/US6420115B1/en not_active Expired - Lifetime
-
2001
- 2001-07-09 BR BR0112400-5A patent/BR0112400A/pt not_active IP Right Cessation
- 2001-07-09 RU RU2003103772/13A patent/RU2273643C2/ru not_active IP Right Cessation
- 2001-07-09 AU AU8000701A patent/AU8000701A/xx active Pending
- 2001-07-09 KR KR20037000309A patent/KR100488826B1/ko not_active IP Right Cessation
- 2001-07-09 AU AU2001280007A patent/AU2001280007B2/en not_active Ceased
- 2001-07-09 CA CA2415493A patent/CA2415493C/en not_active Expired - Fee Related
- 2001-07-09 EP EP01958285A patent/EP1307591A4/en not_active Withdrawn
- 2001-07-09 WO PCT/IB2001/001538 patent/WO2002004655A2/en active IP Right Grant
- 2001-07-09 JP JP2002509508A patent/JP2004511218A/ja active Pending
- 2001-07-09 IL IL15354801A patent/IL153548A0/xx unknown
- 2001-07-09 MX MXPA03000340A patent/MXPA03000340A/es active IP Right Grant
- 2001-07-09 CN CNB018126588A patent/CN1192117C/zh not_active Expired - Fee Related
-
2002
- 2002-03-21 US US10/103,002 patent/US6878815B2/en not_active Expired - Fee Related
- 2002-12-19 IL IL153548A patent/IL153548A/en not_active IP Right Cessation
-
2004
- 2004-06-11 IN IN1656CH2004 patent/IN2004CH01656A/xx unknown
Also Published As
Publication number | Publication date |
---|---|
IL153548A (en) | 2008-12-29 |
MXPA03000340A (es) | 2004-04-05 |
EP1307591A2 (en) | 2003-05-07 |
JP2004511218A (ja) | 2004-04-15 |
RU2273643C2 (ru) | 2006-04-10 |
US20020173480A1 (en) | 2002-11-21 |
IN2004CH01656A (en) | 2006-12-29 |
AU8000701A (en) | 2002-01-21 |
CA2415493A1 (en) | 2002-01-17 |
KR20030066580A (ko) | 2003-08-09 |
CA2415493C (en) | 2010-09-28 |
WO2002004655A3 (en) | 2002-06-27 |
EP1307591A4 (en) | 2005-02-09 |
CN1441850A (zh) | 2003-09-10 |
AU2001280007B2 (en) | 2005-03-24 |
IL153548A0 (en) | 2003-07-06 |
KR100488826B1 (ko) | 2005-05-12 |
US6878815B2 (en) | 2005-04-12 |
US6420115B1 (en) | 2002-07-16 |
WO2002004655A2 (en) | 2002-01-17 |
CN1192117C (zh) | 2005-03-09 |
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Legal Events
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B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 11, 13, 25 DA LPI |
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B12B | Appeal against refusal [chapter 12.2 patent gazette] | ||
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |