MXPA03000340A - Ensayo de hibridizacion triple mediada por cation. - Google Patents

Ensayo de hibridizacion triple mediada por cation.

Info

Publication number
MXPA03000340A
MXPA03000340A MXPA03000340A MXPA03000340A MXPA03000340A MX PA03000340 A MXPA03000340 A MX PA03000340A MX PA03000340 A MXPA03000340 A MX PA03000340A MX PA03000340 A MXPA03000340 A MX PA03000340A MX PA03000340 A MXPA03000340 A MX PA03000340A
Authority
MX
Mexico
Prior art keywords
probe
target
nucleic acid
hybridization assay
label
Prior art date
Application number
MXPA03000340A
Other languages
English (en)
Inventor
Jasmine I Daksis
Original Assignee
Ingeneus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp filed Critical Ingeneus Corp
Publication of MXPA03000340A publication Critical patent/MXPA03000340A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Los complejos triples contienen una sonda de un solo filamento unida a un objetivo de acido nucleico de doble filamento, en el cual la sonda incluye un acido nucleico heteropolimerico o un analogo de acido nucleico heteropolimerico. Todos los tripletes base del complejo son miembros seleccionados a partir del grupo que consiste en A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G y C-G-C. Un ensayo facilitado por cation incluye la deteccion de la presencia de tales complejos triples a fin de determinar el grado de complementaridad entre la sonda y la secuencia objetivo. El ensayo preferentemente detecta un cambio en la intensidad de fluorescencia de una etiqueta como una funcion de afinidad de enlace entre la sonda y el objetivo. La etiqueta puede atarse de manera covalente a la sonda o al objetivo, o puede ser un fluoroforo intercalante en el medio de reaccion.
MXPA03000340A 2000-07-10 2001-07-09 Ensayo de hibridizacion triple mediada por cation. MXPA03000340A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/613,263 US6420115B1 (en) 1999-12-21 2000-07-10 Cation mediated triplex hybridization assay
PCT/IB2001/001538 WO2002004655A2 (en) 2000-07-10 2001-07-09 Cation mediated triplex hybridization assay

Publications (1)

Publication Number Publication Date
MXPA03000340A true MXPA03000340A (es) 2004-04-05

Family

ID=24456561

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA03000340A MXPA03000340A (es) 2000-07-10 2001-07-09 Ensayo de hibridizacion triple mediada por cation.

Country Status (13)

Country Link
US (2) US6420115B1 (es)
EP (1) EP1307591A4 (es)
JP (1) JP2004511218A (es)
KR (1) KR100488826B1 (es)
CN (1) CN1192117C (es)
AU (2) AU2001280007B2 (es)
BR (1) BR0112400A (es)
CA (1) CA2415493C (es)
IL (2) IL153548A0 (es)
IN (1) IN2004CH01656A (es)
MX (1) MXPA03000340A (es)
RU (1) RU2273643C2 (es)
WO (1) WO2002004655A2 (es)

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US6900300B1 (en) * 2000-09-19 2005-05-31 Ingeneus Corporation Quadruplex DNA and duplex probe systems
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6911536B1 (en) * 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US6927027B2 (en) * 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US20040019791A1 (en) * 2002-07-24 2004-01-29 Congruence, Llc Code for object identification
EP1527333A1 (en) * 2002-08-01 2005-05-04 Sensor Technologies LLC Fluorescence correlation spectroscopy instrument
US20040142329A1 (en) * 2003-01-17 2004-07-22 Ingeneus Corporation Probe conjugation to increase multiplex binding motif preference
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
GB0310270D0 (en) * 2003-05-03 2003-06-11 Univ Edinburgh Biomolecular devices
RU2464320C2 (ru) * 2004-09-24 2012-10-20 Инджиниес Инк. Геномный анализ
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WO2006081426A2 (en) * 2005-01-28 2006-08-03 Applera Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template
US20090018028A1 (en) * 2005-05-12 2009-01-15 Stuart Lindsay Self-Assembled Nucleic Acid Nanoarrays and Uses Therefor
WO2009037511A1 (en) * 2007-09-21 2009-03-26 Astrazeneca Ab Assay for dna supercoiling/relaxation

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Also Published As

Publication number Publication date
WO2002004655A3 (en) 2002-06-27
US6420115B1 (en) 2002-07-16
RU2273643C2 (ru) 2006-04-10
EP1307591A4 (en) 2005-02-09
CN1441850A (zh) 2003-09-10
IL153548A0 (en) 2003-07-06
BR0112400A (pt) 2004-07-20
CA2415493C (en) 2010-09-28
IN2004CH01656A (en) 2006-12-29
KR20030066580A (ko) 2003-08-09
AU2001280007B2 (en) 2005-03-24
IL153548A (en) 2008-12-29
KR100488826B1 (ko) 2005-05-12
WO2002004655A2 (en) 2002-01-17
AU8000701A (en) 2002-01-21
EP1307591A2 (en) 2003-05-07
CN1192117C (zh) 2005-03-09
CA2415493A1 (en) 2002-01-17
US20020173480A1 (en) 2002-11-21
JP2004511218A (ja) 2004-04-15
US6878815B2 (en) 2005-04-12

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