DE60005309D1 - Methode zur sequenzierung von polynukleotiden - Google Patents

Methode zur sequenzierung von polynukleotiden

Info

Publication number
DE60005309D1
DE60005309D1 DE60005309T DE60005309T DE60005309D1 DE 60005309 D1 DE60005309 D1 DE 60005309D1 DE 60005309 T DE60005309 T DE 60005309T DE 60005309 T DE60005309 T DE 60005309T DE 60005309 D1 DE60005309 D1 DE 60005309D1
Authority
DE
Germany
Prior art keywords
target
primer
extension products
labeled
hybrid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60005309T
Other languages
English (en)
Other versions
DE60005309T2 (de
Inventor
D Mcginnis
M Madonik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of DE60005309D1 publication Critical patent/DE60005309D1/de
Publication of DE60005309T2 publication Critical patent/DE60005309T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60005309T 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden Expired - Lifetime DE60005309T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/250,422 US6635419B1 (en) 1999-02-16 1999-02-16 Polynucleotide sequencing method
US250422 1999-02-16
PCT/US2000/004034 WO2000049178A2 (en) 1999-02-16 2000-02-16 Polynucleotide sequencing method

Publications (2)

Publication Number Publication Date
DE60005309D1 true DE60005309D1 (de) 2003-10-23
DE60005309T2 DE60005309T2 (de) 2004-06-24

Family

ID=22947677

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60005309T Expired - Lifetime DE60005309T2 (de) 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden

Country Status (8)

Country Link
US (2) US6635419B1 (de)
EP (1) EP1159454B1 (de)
JP (1) JP4113675B2 (de)
AT (1) ATE250142T1 (de)
AU (1) AU764099B2 (de)
CA (1) CA2362245C (de)
DE (1) DE60005309T2 (de)
WO (1) WO2000049178A2 (de)

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US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
EP1776482A2 (de) 2004-06-30 2007-04-25 Applera Corporation Loglineare verstärkung
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CN101946010B (zh) 2007-12-21 2014-08-20 哈佛大学 用于核酸测序的系统和方法
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP2373812B1 (de) 2008-12-19 2016-11-09 President and Fellows of Harvard College Partikel basierte sequenzierung von nucleinsäuren
US9056289B2 (en) 2009-10-27 2015-06-16 President And Fellows Of Harvard College Droplet creation techniques
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2931919B1 (de) 2012-12-14 2019-02-20 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
EP3065712A4 (de) 2013-11-08 2017-06-21 President and Fellows of Harvard College Mikropartikel, verfahren zu deren herstellung und verwendung
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP4235677A3 (de) 2014-06-26 2023-11-22 10X Genomics, Inc. Verfahren und systeme zur nukleinsäuresequenzanordnung
EP3212807B1 (de) 2014-10-29 2020-09-02 10X Genomics, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
AU2016219480B2 (en) 2015-02-09 2021-11-11 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
AU2016338907B2 (en) 2015-10-13 2022-07-07 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN115369161A (zh) 2015-12-04 2022-11-22 10X 基因组学有限公司 用于核酸分析的方法和组合物
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
EP4230746A3 (de) 2017-05-26 2023-11-01 10X Genomics, Inc. Einzelzellanalyse von transposase-zugänglichem chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
US20240344125A1 (en) * 2021-09-06 2024-10-17 Hitachi High-Tech Corporation Nucleotide Sequence Identification Method

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CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
DE69322266T2 (de) 1992-04-03 1999-06-02 Perkin-Elmer Corp., Foster City, Calif. Proben zusammensetzung und verfahren
WO1995024505A1 (en) 1994-03-08 1995-09-14 Amersham International Plc Modifying nucleotide analogues
US5976798A (en) 1994-03-30 1999-11-02 Mitokor Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5656145A (en) * 1996-05-20 1997-08-12 Bio-Rad Laboratories, Inc. Needle guide for loading samples into a vertical slab gel
US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method

Also Published As

Publication number Publication date
US6635419B1 (en) 2003-10-21
WO2000049178A3 (en) 2000-12-14
JP2002536980A (ja) 2002-11-05
EP1159454A2 (de) 2001-12-05
AU3233900A (en) 2000-09-04
ATE250142T1 (de) 2003-10-15
DE60005309T2 (de) 2004-06-24
EP1159454B1 (de) 2003-09-17
WO2000049178A2 (en) 2000-08-24
AU764099B2 (en) 2003-08-07
CA2362245A1 (en) 2000-08-24
US20040063138A1 (en) 2004-04-01
CA2362245C (en) 2008-12-09
JP4113675B2 (ja) 2008-07-09

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

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