DE60005309D1 - Methode zur sequenzierung von polynukleotiden - Google Patents

Methode zur sequenzierung von polynukleotiden

Info

Publication number
DE60005309D1
DE60005309D1 DE60005309T DE60005309T DE60005309D1 DE 60005309 D1 DE60005309 D1 DE 60005309D1 DE 60005309 T DE60005309 T DE 60005309T DE 60005309 T DE60005309 T DE 60005309T DE 60005309 D1 DE60005309 D1 DE 60005309D1
Authority
DE
Germany
Prior art keywords
target
primer
extension products
labeled
hybrid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60005309T
Other languages
English (en)
Other versions
DE60005309T2 (de
Inventor
D Mcginnis
M Madonik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of DE60005309D1 publication Critical patent/DE60005309D1/de
Application granted granted Critical
Publication of DE60005309T2 publication Critical patent/DE60005309T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60005309T 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden Expired - Lifetime DE60005309T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/250,422 US6635419B1 (en) 1999-02-16 1999-02-16 Polynucleotide sequencing method
US250422 1999-02-16
PCT/US2000/004034 WO2000049178A2 (en) 1999-02-16 2000-02-16 Polynucleotide sequencing method

Publications (2)

Publication Number Publication Date
DE60005309D1 true DE60005309D1 (de) 2003-10-23
DE60005309T2 DE60005309T2 (de) 2004-06-24

Family

ID=22947677

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60005309T Expired - Lifetime DE60005309T2 (de) 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden

Country Status (8)

Country Link
US (2) US6635419B1 (de)
EP (1) EP1159454B1 (de)
JP (1) JP4113675B2 (de)
AT (1) ATE250142T1 (de)
AU (1) AU764099B2 (de)
CA (1) CA2362245C (de)
DE (1) DE60005309T2 (de)
WO (1) WO2000049178A2 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
EP1776482A2 (de) 2004-06-30 2007-04-25 Applera Corporation Loglineare verstärkung
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CN101946010B (zh) 2007-12-21 2014-08-20 哈佛大学 用于核酸测序的系统和方法
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3587594B1 (de) 2008-12-19 2022-04-13 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
EP3461558B1 (de) 2009-10-27 2021-03-17 President and Fellows of Harvard College Tröpfchenbildungsverfahren
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (de) 2012-12-14 2019-11-13 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN110211637B (zh) 2014-06-26 2023-10-27 10X基因组学有限公司 核酸序列装配的方法和系统
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
EP4092681A1 (de) 2015-01-13 2022-11-23 10X Genomics, Inc. Systeme und verfahren zur visualisierung von informationen zu struktureller variation und phase
EP3256606B1 (de) 2015-02-09 2019-05-22 10X Genomics, Inc. Systeme und verfahren zur bestimmung struktureller varianten
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271A1 (de) 2018-04-06 2021-02-17 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung
CN117795097A (zh) * 2021-09-06 2024-03-29 株式会社日立高新技术 核苷酸序列确定方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
JP2775346B2 (ja) 1992-04-03 1998-07-16 アプライド バイオシステムズ，インコーポレイテッド プローブ構成物および方法
WO1995024505A1 (en) 1994-03-08 1995-09-14 Amersham International Plc Modifying nucleotide analogues
US5976798A (en) 1994-03-30 1999-11-02 Mitokor Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5656145A (en) * 1996-05-20 1997-08-12 Bio-Rad Laboratories, Inc. Needle guide for loading samples into a vertical slab gel
US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method

Also Published As

Publication number Publication date
CA2362245C (en) 2008-12-09
CA2362245A1 (en) 2000-08-24
US20040063138A1 (en) 2004-04-01
EP1159454B1 (de) 2003-09-17
JP2002536980A (ja) 2002-11-05
JP4113675B2 (ja) 2008-07-09
AU3233900A (en) 2000-09-04
US6635419B1 (en) 2003-10-21
WO2000049178A3 (en) 2000-12-14
ATE250142T1 (de) 2003-10-15
EP1159454A2 (de) 2001-12-05
WO2000049178A2 (en) 2000-08-24
AU764099B2 (en) 2003-08-07
DE60005309T2 (de) 2004-06-24

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

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