ATE227347T1 - Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung - Google Patents
Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendungInfo
- Publication number
- ATE227347T1 ATE227347T1 AT99123807T AT99123807T ATE227347T1 AT E227347 T1 ATE227347 T1 AT E227347T1 AT 99123807 T AT99123807 T AT 99123807T AT 99123807 T AT99123807 T AT 99123807T AT E227347 T1 ATE227347 T1 AT E227347T1
- Authority
- AT
- Austria
- Prior art keywords
- sequencing
- dna molecules
- direct
- exponential amplification
- application
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 238000012163 sequencing technique Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 5
- 102000053602 DNA Human genes 0.000 abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000009609 Pyrophosphatases Human genes 0.000 abstract 1
- 108010009413 Pyrophosphatases Proteins 0.000 abstract 1
- 239000005546 dideoxynucleotide Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000011535 reaction buffer Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/143—Concentration of primer or probe
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19653439A DE19653439A1 (de) | 1996-12-20 | 1996-12-20 | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE227347T1 true ATE227347T1 (de) | 2002-11-15 |
Family
ID=7815636
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97122380T ATE220426T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
| AT99123807T ATE227347T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97122380T ATE220426T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US6107032A (de) |
| EP (2) | EP0849364B1 (de) |
| JP (1) | JPH10295400A (de) |
| AT (2) | ATE220426T1 (de) |
| DE (3) | DE19653439A1 (de) |
| ES (1) | ES2186293T3 (de) |
Families Citing this family (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6214555B1 (en) * | 1996-05-01 | 2001-04-10 | Visible Genetics Inc. | Method compositions and kit for detection |
| DE19653439A1 (de) * | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
| US6828094B2 (en) * | 1996-12-20 | 2004-12-07 | Roche Diagnostics Gmbh | Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application |
| US6605428B2 (en) * | 1996-12-20 | 2003-08-12 | Roche Diagnostics Gmbh | Method for the direct, exponential amplification and sequencing of DNA molecules and its application |
| US20030215857A1 (en) * | 1996-12-20 | 2003-11-20 | Roche Diagnostics Gmbh | Method for the direct, exponential amplification and sequencing of DNA molecules and its application |
| CA2283789C (en) * | 1997-03-12 | 2005-07-05 | Applied Biosystems, Llc | Dna polymerases having improved labeled nucleotide incorporation properties |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| DE19848403A1 (de) * | 1998-10-21 | 2000-04-27 | Guenter Von Kiedrowski | Verfahren zur exponentiellen Amplifikation molekularer Matrizen |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| CA2384838C (en) | 1999-09-13 | 2006-07-18 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| ATE455186T1 (de) * | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| US20030082605A1 (en) * | 2000-09-06 | 2003-05-01 | Hodge Timothy A. | Genomic DNA detection method and system thereof |
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| US20050266494A1 (en) * | 2000-09-06 | 2005-12-01 | Hodge Timothy A | System and method for computer network ordering of biological testing |
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| US20050239125A1 (en) * | 2000-09-06 | 2005-10-27 | Hodge Timothy A | Methods for genotype screening |
| US20030087286A1 (en) * | 2000-09-06 | 2003-05-08 | Hodge Timothy A. | Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles |
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| WO2002048402A2 (en) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
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| KR20030082535A (ko) | 2001-03-09 | 2003-10-22 | 뉴젠 테크놀로지스 인코포레이티드 | Rna 서열의 증폭을 위한 방법 및 조성물 |
| CA2439074A1 (en) * | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| WO2002072892A1 (en) | 2001-03-12 | 2002-09-19 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
| US20060014186A1 (en) * | 2001-09-04 | 2006-01-19 | Hodge Timothy A | Methods for genotype screening of a strain disposed on an adsorbent carrier |
| US20050042629A1 (en) | 2002-09-13 | 2005-02-24 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
| US20030219770A1 (en) * | 2001-11-08 | 2003-11-27 | Eshleman James R. | Methods and systems of nucleic acid sequencing |
| AU2002346498A1 (en) | 2001-11-30 | 2003-06-17 | Applera Corporation | Thermus brockianus nucleic acid polymerases |
| US20040096826A1 (en) * | 2002-01-30 | 2004-05-20 | Evans Glen A. | Methods for creating recombination products between nucleotide sequences |
| WO2003071252A2 (en) | 2002-02-15 | 2003-08-28 | Exact Sciences Corporation | Methods for analysis of molecular events |
| JP2006523465A (ja) | 2003-04-14 | 2006-10-19 | ニューゲン テクノロジーズ, インコーポレイテッド | ランダムにプライミングされる複合プライマーを用いる大規模増幅 |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| EP2248911A1 (de) | 2004-02-19 | 2010-11-10 | Helicos Biosciences Corporation | Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen |
| CA2566806A1 (en) | 2004-05-25 | 2006-01-19 | Helicos Biosciences Corporation | Methods and devices for nucleic acid sequence determination |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| US7692219B1 (en) | 2004-06-25 | 2010-04-06 | University Of Hawaii | Ultrasensitive biosensors |
| JP4476050B2 (ja) * | 2004-06-30 | 2010-06-09 | 株式会社ニデック | 視野計 |
| US7981607B2 (en) | 2004-08-27 | 2011-07-19 | Esoterix Genetic Laboratories LLC | Method for detecting recombinant event |
| US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
| US7785785B2 (en) | 2004-11-12 | 2010-08-31 | The Board Of Trustees Of The Leland Stanford Junior University | Charge perturbation detection system for DNA and other molecules |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| WO2007044071A2 (en) | 2005-04-21 | 2007-04-19 | Exact Sciences Corporation | Analysis of heterogeneous nucleic acid samples |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
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| US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
| CA2704361C (en) * | 2007-11-09 | 2023-04-04 | Genesis Genomics Inc. | Mitochondrial dna deletion between about residues 12317-16254 for use in the detection of cancer |
| US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
| CN102203282B (zh) | 2008-06-25 | 2014-04-30 | 生命技术公司 | 使用大规模fet阵列测量分析物的方法和装置 |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
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| US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
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| US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
| US10379079B2 (en) | 2014-12-18 | 2019-08-13 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP3234576B1 (de) | 2014-12-18 | 2023-11-22 | Life Technologies Corporation | Integrierte schaltung mit hoher datenrate und mit transmitterkonfiguration |
| US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US4962020A (en) * | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
| US5075216A (en) * | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
| US5427911A (en) * | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
| CA2114124A1 (en) * | 1991-07-24 | 1993-02-04 | Keith Gregg | Single step amplification and sequencing of nucleic acids |
| FI923911A7 (fi) * | 1992-09-01 | 1994-03-02 | Timo Korpela | DNA-molekyylien in vitro-synteesi |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5432065A (en) * | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
| US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5830657A (en) * | 1996-05-01 | 1998-11-03 | Visible Genetics Inc. | Method for single-tube sequencing of nucleic acid polymers |
| US5789168A (en) * | 1996-05-01 | 1998-08-04 | Visible Genetics Inc. | Method for amplification and sequencing of nucleic acid polymers |
| US5928906A (en) * | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| DE19653439A1 (de) * | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
-
1996
- 1996-12-20 DE DE19653439A patent/DE19653439A1/de not_active Ceased
-
1997
- 1997-12-16 US US08/991,347 patent/US6107032A/en not_active Expired - Fee Related
- 1997-12-18 AT AT97122380T patent/ATE220426T1/de not_active IP Right Cessation
- 1997-12-18 ES ES99123807T patent/ES2186293T3/es not_active Expired - Lifetime
- 1997-12-18 EP EP97122380A patent/EP0849364B1/de not_active Expired - Lifetime
- 1997-12-18 DE DE69713866T patent/DE69713866T2/de not_active Expired - Fee Related
- 1997-12-18 AT AT99123807T patent/ATE227347T1/de not_active IP Right Cessation
- 1997-12-18 EP EP99123807A patent/EP1004677B1/de not_active Expired - Lifetime
- 1997-12-18 DE DE69716966T patent/DE69716966T2/de not_active Expired - Fee Related
- 1997-12-19 JP JP9351817A patent/JPH10295400A/ja active Pending
-
2001
- 2001-09-20 US US09/956,342 patent/US20020192661A1/en not_active Abandoned
-
2004
- 2004-04-15 US US10/824,465 patent/US20040197811A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE69716966T2 (de) | 2003-08-28 |
| EP1004677A1 (de) | 2000-05-31 |
| DE69713866D1 (de) | 2002-08-14 |
| ES2186293T3 (es) | 2003-05-01 |
| EP0849364A1 (de) | 1998-06-24 |
| US20020192661A1 (en) | 2002-12-19 |
| US6107032A (en) | 2000-08-22 |
| EP0849364B1 (de) | 2002-07-10 |
| US20040197811A1 (en) | 2004-10-07 |
| DE19653439A1 (de) | 1998-07-02 |
| JPH10295400A (ja) | 1998-11-10 |
| DE69713866T2 (de) | 2003-02-06 |
| EP1004677B1 (de) | 2002-11-06 |
| DE69716966D1 (de) | 2002-12-12 |
| ATE220426T1 (de) | 2002-07-15 |
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