ATE227347T1 - Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung - Google Patents
Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendungInfo
- Publication number
- ATE227347T1 ATE227347T1 AT99123807T AT99123807T ATE227347T1 AT E227347 T1 ATE227347 T1 AT E227347T1 AT 99123807 T AT99123807 T AT 99123807T AT 99123807 T AT99123807 T AT 99123807T AT E227347 T1 ATE227347 T1 AT E227347T1
- Authority
- AT
- Austria
- Prior art keywords
- sequencing
- dna molecules
- direct
- exponential amplification
- application
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/143—Concentration of primer or probe
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19653439A DE19653439A1 (de) | 1996-12-20 | 1996-12-20 | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE227347T1 true ATE227347T1 (de) | 2002-11-15 |
Family
ID=7815636
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT99123807T ATE227347T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
AT97122380T ATE220426T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT97122380T ATE220426T1 (de) | 1996-12-20 | 1997-12-18 | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung |
Country Status (6)
Country | Link |
---|---|
US (3) | US6107032A (de) |
EP (2) | EP1004677B1 (de) |
JP (1) | JPH10295400A (de) |
AT (2) | ATE227347T1 (de) |
DE (3) | DE19653439A1 (de) |
ES (1) | ES2186293T3 (de) |
Families Citing this family (82)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6214555B1 (en) * | 1996-05-01 | 2001-04-10 | Visible Genetics Inc. | Method compositions and kit for detection |
US6605428B2 (en) * | 1996-12-20 | 2003-08-12 | Roche Diagnostics Gmbh | Method for the direct, exponential amplification and sequencing of DNA molecules and its application |
US20030215857A1 (en) * | 1996-12-20 | 2003-11-20 | Roche Diagnostics Gmbh | Method for the direct, exponential amplification and sequencing of DNA molecules and its application |
US6828094B2 (en) * | 1996-12-20 | 2004-12-07 | Roche Diagnostics Gmbh | Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application |
DE19653439A1 (de) * | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
JP3435416B2 (ja) * | 1997-03-12 | 2003-08-11 | ピーイー コーポレイション(エヌワイ) | 改善された標識ヌクレオチド取込み特性を有するdnaポリメラーゼ |
US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
DE19848403A1 (de) * | 1998-10-21 | 2000-04-27 | Guenter Von Kiedrowski | Verfahren zur exponentiellen Amplifikation molekularer Matrizen |
DE60009323T2 (de) | 1999-09-13 | 2005-02-10 | Nugen Technologies, Inc., San Carlos | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
AU2001271595A1 (en) * | 2000-06-26 | 2002-01-08 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US20030082605A1 (en) * | 2000-09-06 | 2003-05-01 | Hodge Timothy A. | Genomic DNA detection method and system thereof |
US7011943B2 (en) | 2000-09-06 | 2006-03-14 | Transnetyx, Inc. | Method for detecting a designated genetic sequence in murine genomic DNA |
US20050239125A1 (en) * | 2000-09-06 | 2005-10-27 | Hodge Timothy A | Methods for genotype screening |
US20050272085A1 (en) * | 2000-09-06 | 2005-12-08 | Hodge Timothy A | Methods for forensic and congenic screening |
US20030087286A1 (en) * | 2000-09-06 | 2003-05-08 | Hodge Timothy A. | Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles |
US7494817B2 (en) * | 2000-09-06 | 2009-02-24 | Transnet Yx, Inc. | Methods for genotype screening using magnetic particles |
US20050266494A1 (en) * | 2000-09-06 | 2005-12-01 | Hodge Timothy A | System and method for computer network ordering of biological testing |
WO2002048402A2 (en) | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
US7060434B2 (en) * | 2001-02-14 | 2006-06-13 | Council Of Scientific & Industrial Research | Probes for myctophid fish and a method for developing the same |
JP4542312B2 (ja) | 2001-03-09 | 2010-09-15 | ニューゲン テクノロジーズ, インコーポレイテッド | Rna配列の増幅のための方法および組成物 |
CA2439074A1 (en) * | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
US20060014186A1 (en) * | 2001-09-04 | 2006-01-19 | Hodge Timothy A | Methods for genotype screening of a strain disposed on an adsorbent carrier |
US20050042629A1 (en) | 2002-09-13 | 2005-02-24 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
WO2003056030A2 (en) * | 2001-11-08 | 2003-07-10 | The Johns Hopkins University | Methods and systems of nucleic acid sequencing |
AU2002346498A1 (en) | 2001-11-30 | 2003-06-17 | Applera Corporation | Thermus brockianus nucleic acid polymerases |
US20040096826A1 (en) * | 2002-01-30 | 2004-05-20 | Evans Glen A. | Methods for creating recombination products between nucleotide sequences |
US7776524B2 (en) | 2002-02-15 | 2010-08-17 | Genzyme Corporation | Methods for analysis of molecular events |
CA2521084A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
ATE463584T1 (de) | 2004-02-19 | 2010-04-15 | Helicos Biosciences Corp | Verfahren zur analyse von polynukleotidsequenzen |
US8536661B1 (en) | 2004-06-25 | 2013-09-17 | University Of Hawaii | Biosensor chip sensor protection methods |
JP4476050B2 (ja) * | 2004-06-30 | 2010-06-09 | 株式会社ニデック | 視野計 |
WO2006026654A2 (en) | 2004-08-27 | 2006-03-09 | Exact Sciences Corporation | Method for detecting a recombinant event |
US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
US7785785B2 (en) | 2004-11-12 | 2010-08-31 | The Board Of Trustees Of The Leland Stanford Junior University | Charge perturbation detection system for DNA and other molecules |
US9777314B2 (en) | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US7939258B2 (en) | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
EP4134667A1 (de) * | 2006-12-14 | 2023-02-15 | Life Technologies Corporation | Vorrichtungen zur messung von analyten unter verwendung von fet-arrays |
US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US7932034B2 (en) * | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
AU2008324675B2 (en) * | 2007-11-09 | 2015-04-23 | Mdna Life Sciences Inc. | Mitochondrial DNA deletion between about residues 12317-16254 for use in the detection of cancer |
US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
JP5667049B2 (ja) | 2008-06-25 | 2015-02-12 | ライフ テクノロジーズ コーポレーション | 大規模なfetアレイを用いて分析物を測定するための方法および装置 |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
US8776573B2 (en) | 2009-05-29 | 2014-07-15 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US20120261274A1 (en) | 2009-05-29 | 2012-10-18 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US8823380B2 (en) | 2010-06-30 | 2014-09-02 | Life Technologies Corporation | Capacitive charge pump |
US8858782B2 (en) | 2010-06-30 | 2014-10-14 | Life Technologies Corporation | Ion-sensing charge-accumulation circuits and methods |
CN106932456B (zh) | 2010-06-30 | 2020-02-21 | 生命科技公司 | 用于测试isfet阵列的方法和装置 |
US11307166B2 (en) | 2010-07-01 | 2022-04-19 | Life Technologies Corporation | Column ADC |
US8653567B2 (en) | 2010-07-03 | 2014-02-18 | Life Technologies Corporation | Chemically sensitive sensor with lightly doped drains |
US9618475B2 (en) | 2010-09-15 | 2017-04-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US8796036B2 (en) | 2010-09-24 | 2014-08-05 | Life Technologies Corporation | Method and system for delta double sampling |
DE102011083555B4 (de) | 2011-09-27 | 2013-10-10 | Aspre Ag | Analyseverfahren und Analysevorrichtung |
US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
US8821798B2 (en) | 2012-01-19 | 2014-09-02 | Life Technologies Corporation | Titanium nitride as sensing layer for microwell structure |
US8747748B2 (en) | 2012-01-19 | 2014-06-10 | Life Technologies Corporation | Chemical sensor with conductive cup-shaped sensor surface |
US8786331B2 (en) | 2012-05-29 | 2014-07-22 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US9080968B2 (en) | 2013-01-04 | 2015-07-14 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
US8962366B2 (en) | 2013-01-28 | 2015-02-24 | Life Technologies Corporation | Self-aligned well structures for low-noise chemical sensors |
US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
US8841217B1 (en) | 2013-03-13 | 2014-09-23 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
CN105283758B (zh) | 2013-03-15 | 2018-06-05 | 生命科技公司 | 具有一致传感器表面区域的化学传感器 |
WO2014149780A1 (en) | 2013-03-15 | 2014-09-25 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
CN105051525B (zh) | 2013-03-15 | 2019-07-26 | 生命科技公司 | 具有薄导电元件的化学设备 |
US20140336063A1 (en) | 2013-05-09 | 2014-11-13 | Life Technologies Corporation | Windowed Sequencing |
US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
TWI832669B (zh) | 2014-12-18 | 2024-02-11 | 美商生命技術公司 | 具有傳輸器組態的高資料速率積體電路 |
KR20170097712A (ko) | 2014-12-18 | 2017-08-28 | 라이프 테크놀로지스 코포레이션 | 대형 fet 어레이를 사용한 분석물 측정을 위한 방법과 장치 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
US4962020A (en) * | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
US5075216A (en) * | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
US5427911A (en) * | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
EP0595982A4 (en) * | 1991-07-24 | 1995-10-11 | Univ Partnerships Pty Ltd | Single step amplification and sequencing of nucleic acids |
FI923911A (fi) * | 1992-09-01 | 1994-03-02 | Vsevolod Kiselev | DNA-molekylers in vitro-syntes |
US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
US5432065A (en) * | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
US5789168A (en) * | 1996-05-01 | 1998-08-04 | Visible Genetics Inc. | Method for amplification and sequencing of nucleic acid polymers |
US5830657A (en) * | 1996-05-01 | 1998-11-03 | Visible Genetics Inc. | Method for single-tube sequencing of nucleic acid polymers |
US5928906A (en) * | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
DE19653439A1 (de) * | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
-
1996
- 1996-12-20 DE DE19653439A patent/DE19653439A1/de not_active Ceased
-
1997
- 1997-12-16 US US08/991,347 patent/US6107032A/en not_active Expired - Fee Related
- 1997-12-18 ES ES99123807T patent/ES2186293T3/es not_active Expired - Lifetime
- 1997-12-18 DE DE69713866T patent/DE69713866T2/de not_active Expired - Fee Related
- 1997-12-18 EP EP99123807A patent/EP1004677B1/de not_active Expired - Lifetime
- 1997-12-18 EP EP97122380A patent/EP0849364B1/de not_active Expired - Lifetime
- 1997-12-18 AT AT99123807T patent/ATE227347T1/de not_active IP Right Cessation
- 1997-12-18 DE DE69716966T patent/DE69716966T2/de not_active Expired - Fee Related
- 1997-12-18 AT AT97122380T patent/ATE220426T1/de not_active IP Right Cessation
- 1997-12-19 JP JP9351817A patent/JPH10295400A/ja active Pending
-
2001
- 2001-09-20 US US09/956,342 patent/US20020192661A1/en not_active Abandoned
-
2004
- 2004-04-15 US US10/824,465 patent/US20040197811A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US6107032A (en) | 2000-08-22 |
US20040197811A1 (en) | 2004-10-07 |
DE69713866D1 (de) | 2002-08-14 |
DE69713866T2 (de) | 2003-02-06 |
DE19653439A1 (de) | 1998-07-02 |
JPH10295400A (ja) | 1998-11-10 |
EP1004677A1 (de) | 2000-05-31 |
DE69716966D1 (de) | 2002-12-12 |
DE69716966T2 (de) | 2003-08-28 |
EP0849364B1 (de) | 2002-07-10 |
EP0849364A1 (de) | 1998-06-24 |
ATE220426T1 (de) | 2002-07-15 |
ES2186293T3 (es) | 2003-05-01 |
US20020192661A1 (en) | 2002-12-19 |
EP1004677B1 (de) | 2002-11-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE227347T1 (de) | Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung | |
DE69702333D1 (de) | Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung | |
DE69725076D1 (de) | Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren | |
DE69636080D1 (de) | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren | |
DE69333891D1 (de) | Verfahren zur Nukleinsäurenamplifizierung, Zusammensetzung und Kit | |
DE69708865D1 (de) | Verfahren zur direkten sequenzierung während "template"-amplifikation | |
ATE191934T1 (de) | Verfahren zur isothermer amplifikation von nukleinsäure molekülen | |
ATE159762T1 (de) | Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus | |
ATE315081T1 (de) | Amplifizierung von langen nukleinsäuresequenzen mit pcr | |
DE69523360D1 (de) | Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung | |
DE69034177D1 (de) | Verfahren zur Amplifikation von Nukleinsäuresequenzen | |
ATE244759T1 (de) | Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation | |
DE3750774D1 (de) | Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. | |
ATE266102T1 (de) | Verfahren zur koamplifikation einer zielnukleinsäure unter versendung eines volumenabscheidungsmittel in der reaktionszusammensetzung, testsatz und testvorrichtung | |
ATE184321T1 (de) | Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen | |
DE69428167D1 (de) | Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion | |
DE69834506D1 (de) | Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung | |
DE50008101D1 (de) | Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens | |
ATE197481T1 (de) | Verfahren und reagenzien zur typisierung von hla- genen der klasse i | |
DK0704540T3 (da) | Sortsklassifikationsfremgangsmåde til byg eller malt under anvendelse af gendiagnose og den dertil anvendte primer | |
DE60015210D1 (de) | Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung | |
KR950023714A (ko) | 중합 효소 연쇄 반응 조성물 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 1004677 Country of ref document: EP |
|
REN | Ceased due to non-payment of the annual fee |