WO1996033284A3 - Nucleic acid sequencing - Google Patents

Nucleic acid sequencing Download PDF

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Publication number
WO1996033284A3
WO1996033284A3 PCT/US1996/004883 US9604883W WO9633284A3 WO 1996033284 A3 WO1996033284 A3 WO 1996033284A3 US 9604883 W US9604883 W US 9604883W WO 9633284 A3 WO9633284 A3 WO 9633284A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sequencing
subsets
acid sequence
template
Prior art date
Application number
PCT/US1996/004883
Other languages
French (fr)
Other versions
WO1996033284A2 (en
Inventor
Jonathan Briggs
Randy M Mccormick
Original Assignee
Seurat Analytical Systems Inc
Jonathan Briggs
Randy M Mccormick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seurat Analytical Systems Inc, Jonathan Briggs, Randy M Mccormick filed Critical Seurat Analytical Systems Inc
Priority to AU55390/96A priority Critical patent/AU5539096A/en
Publication of WO1996033284A2 publication Critical patent/WO1996033284A2/en
Publication of WO1996033284A3 publication Critical patent/WO1996033284A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates to a method for sequencing nucleic acids, specifically sequencing DNA using primer extension reactions with deoxynucleoside triphosphates (dNTP's), dideoxynucleoside triphosphate terminators (ddNTP's), primers, and polymerase enzymes. The invention describes methods of generating slightly overlapping and rather narrow size-distribution subsets of sequencing fragments corresponding to contiguous regions along the sequence of the template nucleic acid. These subsets of overlapping contiguous sequencing fragments are rapidly separated in parallel on a set of electrophoresis runs, each of which is optimized to adequately resolve one of the distributions of sequencing fragments in minimal time. The subsets of nucleic acid sequence information generated from the set of electrophoretic separations allows reconstruction of the nucleic acid sequence of the template.
PCT/US1996/004883 1995-04-12 1996-04-11 Nucleic acid sequencing WO1996033284A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU55390/96A AU5539096A (en) 1995-04-12 1996-04-11 Nucleic acid sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42075095A 1995-04-12 1995-04-12
US08/420,750 1995-04-12

Publications (2)

Publication Number Publication Date
WO1996033284A2 WO1996033284A2 (en) 1996-10-24
WO1996033284A3 true WO1996033284A3 (en) 1997-03-06

Family

ID=23667692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/004883 WO1996033284A2 (en) 1995-04-12 1996-04-11 Nucleic acid sequencing

Country Status (2)

Country Link
AU (1) AU5539096A (en)
WO (1) WO1996033284A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001521622A (en) * 1997-04-04 2001-11-06 カリパー テクノロジーズ コーポレイション Closed-loop biochemical analyzer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994017207A1 (en) * 1993-01-21 1994-08-04 Applied Biosystems, Inc. High resolution dna sequencing method using low viscosity medium

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994017207A1 (en) * 1993-01-21 1994-08-04 Applied Biosystems, Inc. High resolution dna sequencing method using low viscosity medium

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HUNKAPILLER T ET AL: "LARGE-SCALE AND AUTOMATED DNA SEQUENCE DETERMINATION", SCIENCE, vol. 254, no. 5028, 4 October 1991 (1991-10-04), pages 59 - 67, XP000371787 *
LUCKEY J A ET AL: "HIGH SPEED DNA SEQUENCING BY CAPILLARY ELECTROPHORESIS", NUCLEIC ACIDS RESEARCH, vol. 18, no. 15, 11 August 1990 (1990-08-11), pages 4417 - 4421, XP000137582 *
MATHIES AND HUANG: "Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing", NATURE, vol. 359, 1992, pages 167 - 169, XP002023300 *
SURAJ P BHAT: "DNA SEQUENCING ON MINIGELS: AN ALTERNATIVE TECHNIQUE FOR ROUTINE ANALYSES", ANALYTICAL BIOCHEMISTRY, vol. 212, no. 2, 1 August 1993 (1993-08-01), pages 560 - 562, XP000383290 *
ZAGURSKY AND MCCORMICK: "DNA sequencing separations in capillary gels on a modified commercial DNA sequencing instrument", BIOTECHNIQUES, vol. 9, no. 1, 1990, pages 74 - 78, XP000616243 *

Also Published As

Publication number Publication date
WO1996033284A2 (en) 1996-10-24
AU5539096A (en) 1996-11-07

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