DE60315043T2 - Nachweis biologischer moleküle mittels differentieller aufteilung von enzymsubstraten und -produkten - Google Patents

Nachweis biologischer moleküle mittels differentieller aufteilung von enzymsubstraten und -produkten Download PDF

Info

Publication number
DE60315043T2
DE60315043T2 DE60315043T DE60315043T DE60315043T2 DE 60315043 T2 DE60315043 T2 DE 60315043T2 DE 60315043 T DE60315043 T DE 60315043T DE 60315043 T DE60315043 T DE 60315043T DE 60315043 T2 DE60315043 T2 DE 60315043T2
Authority
DE
Germany
Prior art keywords
substrate
enzyme
sample
fluorescence
biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60315043T
Other languages
German (de)
English (en)
Other versions
DE60315043D1 (de
Inventor
Stephen R. Kingston BROWN
Samir P. Pickering TABASH
Igor S. Kingston KOZIN
Eric J.-P. Kingston MARCOTTE
Arthur N. Kingston LEY
Kevin R. Kingston HALL
Moe Kanata HUSSAIN
Peter V. Kingston HODSON
Parveen Kingston AKHTAR
John G. Cornwall ST.MARSEILLE
Robin A. Kingston WYNNE-EDWARDS
Raymond J. Bath BOWERS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Queens University at Kingston
Original Assignee
Queens University at Kingston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Queens University at Kingston filed Critical Queens University at Kingston
Publication of DE60315043D1 publication Critical patent/DE60315043D1/de
Application granted granted Critical
Publication of DE60315043T2 publication Critical patent/DE60315043T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE60315043T 2002-09-20 2003-09-22 Nachweis biologischer moleküle mittels differentieller aufteilung von enzymsubstraten und -produkten Expired - Lifetime DE60315043T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41201502P 2002-09-20 2002-09-20
US412015P 2002-09-20
PCT/CA2003/001439 WO2004027084A1 (en) 2002-09-20 2003-09-22 Detection of biological molecules by differential partitioning of enzyme substrates and products

Publications (2)

Publication Number Publication Date
DE60315043D1 DE60315043D1 (de) 2007-08-30
DE60315043T2 true DE60315043T2 (de) 2008-04-10

Family

ID=32030780

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60315043T Expired - Lifetime DE60315043T2 (de) 2002-09-20 2003-09-22 Nachweis biologischer moleküle mittels differentieller aufteilung von enzymsubstraten und -produkten

Country Status (10)

Country Link
US (4) US7402426B2 (https=)
EP (1) EP1539986B1 (https=)
JP (2) JP4857434B2 (https=)
AT (1) ATE367449T1 (https=)
AU (1) AU2003266899B2 (https=)
CA (1) CA2497555C (https=)
DE (1) DE60315043T2 (https=)
ES (1) ES2290487T3 (https=)
MX (1) MXPA05003068A (https=)
WO (1) WO2004027084A1 (https=)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7402426B2 (en) 2002-09-20 2008-07-22 Queen's University At Kingston Detection of biological molecules by differential partitioning of enzyme substrates and products
US7964390B2 (en) * 2002-10-11 2011-06-21 Case Western Reserve University Sensor system
US20070026426A1 (en) 2005-04-26 2007-02-01 Applera Corporation System for genetic surveillance and analysis
EP1926825A4 (en) * 2005-09-20 2008-09-24 Univ Arizona State METHODS FOR DETECTING AND CHARACTERIZING MICROORGANISMS
US7812939B2 (en) * 2006-02-13 2010-10-12 Thermo Electron Scientific Instruments Llc Spectrometric measurements during blending / mixing
JP5374809B2 (ja) * 2006-06-29 2013-12-25 パナソニック株式会社 微生物計測システム
US10213164B2 (en) 2008-09-26 2019-02-26 Qualcomm Incorporated Method and apparatus for under-sampled acquisition and transmission of photoplethysmograph (PPG) data and reconstruction of full band PPG data at the receiver
EP2404162B1 (en) 2009-03-03 2017-12-27 Access Medical System Co., Ltd. Detection system and method for high sensitivity fluorescent assays
MX338624B (es) 2009-05-15 2016-04-26 Bio Merieux Inc Aparato automatizado para la deteccion microbiana.
JP2012526996A (ja) 2009-05-15 2012-11-01 ビオメリュー・インコーポレイテッド 試料内の微生物因子の迅速な同定および／または特徴付けのためのシステムおよび方法
US8891086B2 (en) 2009-06-15 2014-11-18 Thermo Scientific Portable Analytical Instruments Inc. Optical scanning systems and methods for measuring a sealed container with a layer for reducing diffusive scattering
FR2949560A1 (fr) * 2009-09-02 2011-03-04 Centre Nat Rech Scient Systeme de spectroscopie a guide d'onde pour l'analyse de particules dans un milieu
JP5747043B2 (ja) * 2009-12-22 2015-07-08 スリーエム イノベイティブ プロパティズ カンパニー 微生物を検出する方法及びそのためのキット
AU2011267803A1 (en) * 2010-06-18 2013-01-31 Pathogen Detection Systems, Inc. An optical pathogen detection system and quality control materials for use in same
CA2802724C (en) 2010-06-18 2016-09-06 Pathogen Detection Systems, Inc. Method and system for detecting biological molecules in samples
JP5621846B2 (ja) * 2010-06-21 2014-11-12 コニカミノルタ株式会社 蛍光測定方法
BR112013010096B1 (pt) 2010-11-01 2020-03-10 3M Innovative Properties Company Método de detecção de uma atividade biológica
CN103189524B (zh) 2010-11-01 2015-01-28 3M创新有限公司 生物灭菌指示器及其使用方法
US9435739B2 (en) 2010-11-24 2016-09-06 3M Innovative Properties Company Methods and compositions to detect a biological activity
US9164086B2 (en) 2011-10-18 2015-10-20 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques
KR101343353B1 (ko) 2012-05-04 2013-12-20 한국과학기술연구원 엔테로박터 및 에스케리키아의 구별 방법
WO2014093926A1 (en) * 2012-12-14 2014-06-19 Larson Bryan Dual medicament carpule for dental syringes
EP2954102B1 (en) * 2013-02-08 2018-12-19 Bio-Rad Laboratories, Inc. Affinity-based partition assay for detection of target molecules
CN104034705B (zh) * 2013-03-06 2017-05-10 常州欣宏科生物化学有限公司 一种荧光探针检测酶活性方法
JP6567274B2 (ja) * 2014-12-10 2019-08-28 水ｉｎｇ株式会社 分離膜の汚染状態分析方法、その方法を用いるろ過対象水の水質評価方法
KR20240160238A (ko) * 2015-08-03 2024-11-08 와이에스아이 인코포레이티드 다중 파라미터 수질 모니터링을 위한 다중 여기-다중 방출 형광계
US11656180B2 (en) 2015-08-03 2023-05-23 Ysi, Inc. Multi excitation-multi emission fluorometer for multiparameter water quality monitoring
CA3102240A1 (en) * 2018-06-01 2019-12-05 Orb Xyz, Inc. Detecting an analyte in a medium
JP6935883B2 (ja) * 2018-07-13 2021-09-15 五稜化薬株式会社 蛍光画像診断の前処理方法
CN110763823B (zh) * 2019-11-22 2022-04-12 威海精讯畅通电子科技有限公司 一种手持式土壤快速检测仪及检测方法
CN114428070B (zh) * 2021-12-28 2024-05-17 南京师范大学 一种检测革兰氏阴性菌的分子印迹荧光纳米粒子及其制备方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2856251A1 (de) * 1978-12-27 1980-07-17 Max Planck Gesellschaft Fuellungen fuer indikatorraeume
DK256581A (da) * 1980-06-13 1981-12-14 Nat Res Dev Bindingsanalyse
GB2078370A (en) 1980-06-13 1982-01-06 Nat Res Dev Binding assays
AT377095B (de) * 1982-11-23 1985-02-11 List Hans Sensorelement zur bestimmung des o2-gehaltes einer probe sowie verfahren zur herstellung desselben
US5238809A (en) * 1986-02-03 1993-08-24 Avl Medical Instruments Ag Method for optical determination of the catalytic enzyme activity and arrangement for implementing this method
AT392539B (de) * 1986-02-03 1991-04-25 Avl Verbrennungskraft Messtech Verfahren zur optischen bestimmung der katalytischen enzymaktivitaet einer probe, und eine anordnung zur durchfuehrung des verfahrens
JPS62203047A (ja) * 1986-02-28 1987-09-07 Advance:Kk アンモニアセンサ−
US4712865A (en) * 1987-01-05 1987-12-15 Baxter Travenol Laboratories Dye containing silicon polymer composition
JPH0814541B2 (ja) * 1987-04-03 1996-02-14 ティーディーケイ株式会社 光フアイバ−センサ−
JPS6463842A (en) * 1987-09-03 1989-03-09 Terumo Corp Method and apparatus for measuring concentration of optical material
US4974929A (en) * 1987-09-22 1990-12-04 Baxter International, Inc. Fiber optical probe connector for physiologic measurement devices
FR2650076B1 (fr) * 1989-07-20 1991-10-04 Commissariat Energie Atomique Capteur chimique actif a fibre optique et son procede de fabrication
JP3065639B2 (ja) 1990-08-22 2000-07-17 三菱電線工業株式会社 ケーブル事故検出装置
US5376551A (en) * 1991-03-12 1994-12-27 University Of Utah Research Foundation Apparatus for using fluorescently labeled ligands in studying interaction of a native ligand and its receptor
WO1992016648A1 (en) * 1991-03-18 1992-10-01 Environmental Test Systems, Inc. Chemiluminescent test for microorganisms
JP3048672B2 (ja) * 1991-04-30 2000-06-05 栄研化学株式会社 試薬結合ポリマー、該ポリマー膜および該ポリマーを含む担持体
GB9116909D0 (en) 1991-08-06 1991-09-18 Salford Ultrafine Chem & Res Morphine derivatives
GB9215973D0 (en) * 1992-07-28 1992-09-09 Univ Manchester Sensor devices
US5364767A (en) * 1993-02-11 1994-11-15 Research Organics, In. Chromogenic compounds and methods of using same
GB9315183D0 (en) * 1993-07-22 1993-09-08 British Nuclear Fuels Plc Biosensors
JPH07147997A (ja) * 1993-11-26 1995-06-13 Apere:Kk 微生物検査方法および微生物検査装置
DE69519747T2 (de) * 1994-11-07 2001-08-09 Studie- En Samenwerkingverband Vlaams Water, Antwerpen Enzymatisches verfahren zum nachweis coliformer bakterien oder e.coli
CA2232714A1 (en) * 1995-09-29 1997-04-03 Mark W. Pierson Self-contained incubator for growth of microorganism
DE69633573T2 (de) * 1995-11-22 2005-10-06 Medtronic MiniMed, Inc., Northridge Detektion von biologischen molekülen unter verwendung von chemischer amplifikation und optischem sensor
JPH09152433A (ja) * 1995-11-30 1997-06-10 Aisin Seiki Co Ltd 固相担体に固定した核酸等の検出方法
US5972595A (en) * 1997-12-19 1999-10-26 Nen Life Science Products, Inc. Enzyme assay using a solid phase substrate
US6372895B1 (en) * 2000-07-07 2002-04-16 3M Innovative Properties Company Fluorogenic compounds
JP3759039B2 (ja) * 2000-05-11 2006-03-22 松下エコシステムズ株式会社 化学センサデバイス
WO2002074694A2 (en) * 2001-03-16 2002-09-26 Ewatertek Inc. System and method for monitoring water quality and transmitting water quality data
EP1519678A2 (en) * 2001-10-02 2005-04-06 Alfred E. Mann Institute for Biomedical Engineering at the University of Southern California Internal biochemical sensing device
CA2469845A1 (en) * 2001-12-06 2003-06-19 Purdue Research Foundation Mesoporous membrane collector and separator for airborne pathogen detection
EP1493015A4 (en) * 2002-04-05 2006-01-04 Powerzyme Inc ANALYTE DETECTOR
US20040047535A1 (en) * 2002-09-09 2004-03-11 Ljerka Ukrainczyk Enhanced fiber-optic sensor
US7402426B2 (en) 2002-09-20 2008-07-22 Queen's University At Kingston Detection of biological molecules by differential partitioning of enzyme substrates and products

Also Published As

Publication number Publication date
US7402426B2 (en) 2008-07-22
MXPA05003068A (es) 2005-06-08
ATE367449T1 (de) 2007-08-15
US20130217041A1 (en) 2013-08-22
DE60315043D1 (de) 2007-08-30
EP1539986A1 (en) 2005-06-15
AU2003266899A1 (en) 2004-04-08
WO2004027084A1 (en) 2004-04-01
CA2497555A1 (en) 2004-04-01
US20040106164A1 (en) 2004-06-03
AU2003266899B2 (en) 2008-02-28
JP2005538728A (ja) 2005-12-22
EP1539986B1 (en) 2007-07-18
US8377686B2 (en) 2013-02-19
US8632966B2 (en) 2014-01-21
US20140295403A1 (en) 2014-10-02
HK1080514A1 (en) 2006-04-28
US20090117600A1 (en) 2009-05-07
CA2497555C (en) 2013-06-11
ES2290487T3 (es) 2008-02-16
JP5799353B2 (ja) 2015-10-21
JP4857434B2 (ja) 2012-01-18
JP2011254816A (ja) 2011-12-22

Similar Documents

Publication Publication Date Title
DE60315043T2 (de) Nachweis biologischer moleküle mittels differentieller aufteilung von enzymsubstraten und -produkten
DE69211895T2 (de) Vorrichtung zur Überwachung mikrobieller Aktivität
DE68923720T2 (de) Einrichtung und Gerät für die Detektion von Mikroorganismen.
DE3781170T2 (de) Hydrolysierbare fluoreszierende substrate und deren verwendung zur analytischen bestimmung.
DE69020555T2 (de) Fällungstest für mikroorganismen.
DE10101576B4 (de) Optischer Sensor und Sensorfeld
DE69816663T2 (de) Vorrichtung zur bestimmung von analyten in lösungen
DE69718245T2 (de) Druckempfindliches klebendes Blatt zum Nachweis von Mikroorganismen und Methode zum Nachweis von Mikroorganismen
DE69018567T2 (de) Verfahren zum Nachweis von Mikroorganismen durch Verwendung von Resorufin oder Orcirufin.
DE69604574T2 (de) Verfahren zum Nachweis von Mikroorganismen durch Trennung und Kultivierung auf einem Gelsystem, Gelsystem und Testsatz zur Durchführung dieses Verfahrens
DE69720248T2 (de) Schneller mikrobieller test
CH639204A5 (de) Kunststofftraeger zur durchfuehrung von analytischen oder diagnostischen untersuchungen.
DE3903777A1 (de) Verfahren zur schnellen detektion von mikroorganismen in proben und vorrichtung zur durchfuehrung des verfahrens
DE69610537T2 (de) Verfahren zum Nachweis von Mikroorganismen
DE3889765T2 (de) Reagens und Verfahren zur Bestimmung von Kationen.
DE69737718T2 (de) Nachweis von mikroorganismen
DE69331580T2 (de) Nachweis von beweglichen zellen in einer probe
WO2003036293A1 (de) Methode zur gleichzeitigen optischen bestimmung von ph-wert und gelöstsauerstoff
DE3784702T2 (de) Verfahren und vorrichtung zum nachweis und zur mengenbestimmung von bakterien.
DE19617338A1 (de) Verfahren und Vorrichtung zur Identifikation von Mikroorganismen, Pilzen und Kleinstlebewesen mit Indikatorkugeln
HK1080514B (en) Detection of biological molecules by differential partitioning of enzyme substrates and products
EP3904497B1 (de) Testsystem zur bestimmung der enzymatischen aktivität von bakterien auf ammoniak
Class et al. Patent application title: DETECTION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL PARTITIONING OF ENZYME SUBSTRATES AND PRODUCTS
DE60031549T2 (de) Interne qualitätskontrolle
JPH11346758A (ja) 微生物同定試験におけるグラム鑑別用の対照菌株を塗布したスライドグラス

Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: BROWN, STEPHEN R., KINGSTON, ONTARIO K7L 3Y6, CA

Inventor name: TABASH, SAMIR P., PICKERING, ONTARIO L1V 6Z4, CA

Inventor name: KOZIN, IGOR S., KINGSTON, ONTARIO K7M 1J2, CA

Inventor name: MARCOTTE, ERIC J.-P., KINGSTON, ONTARIO K7L 3Y, CA

Inventor name: LEY, ARTHUR N., KINGSTON, ONTARIO K7M 2V8, CA

Inventor name: HALL, KEVIN R., KINGSTON, ONTARIO K7L 4V1, CA

Inventor name: HUSSAIN, MOE, KANATA, ONTARIO K7W 1A2, CA

Inventor name: HODSON, PETER V., KINGSTON, ONTARIO K7L 4E6, CA

Inventor name: AKHTAR, PARVEEN, KINGSTON, ONTARIO K7L 3X1, CA

Inventor name: ST.MARSEILLE, JOHN G., CORNWALL, ONTARIO K6J 1, CA

Inventor name: WYNNE-EDWARDS, ROBIN A., KINGSTON, ONTARIO K7L, CA

Inventor name: BOWERS, RAYMOND J., BATH, ONTARIO K0G 1G0, CA

8364 No opposition during term of opposition