DE60220018T2 - Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung - Google Patents

Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Download PDF

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Publication number
DE60220018T2
DE60220018T2 DE60220018T DE60220018T DE60220018T2 DE 60220018 T2 DE60220018 T2 DE 60220018T2 DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T2 DE60220018 T2 DE 60220018T2
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DE
Germany
Prior art keywords
dextran sulfate
hybridization
molecular weight
probe
tissue section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60220018T
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German (de)
English (en)
Other versions
DE60220018D1 (de
Inventor
Joseph Tucson UTERMOHLEN
Catherine Wolf
Kimberly Tucson CHRISTENSEN
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of DE60220018D1 publication Critical patent/DE60220018D1/de
Application granted granted Critical
Publication of DE60220018T2 publication Critical patent/DE60220018T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Saccharide Compounds (AREA)
DE60220018T 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Expired - Fee Related DE60220018T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/772,123 US6656685B2 (en) 2001-01-29 2001-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use
US772123 2001-01-29
PCT/US2002/002642 WO2002061139A2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Publications (2)

Publication Number Publication Date
DE60220018D1 DE60220018D1 (de) 2007-06-21
DE60220018T2 true DE60220018T2 (de) 2008-01-10

Family

ID=25093987

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60220018T Expired - Fee Related DE60220018T2 (de) 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung

Country Status (8)

Country Link
US (2) US6656685B2 (enExample)
EP (1) EP1356116B1 (enExample)
JP (1) JP2005503762A (enExample)
AT (1) ATE361995T1 (enExample)
AU (1) AU2002240180B2 (enExample)
CA (1) CA2434854A1 (enExample)
DE (1) DE60220018T2 (enExample)
WO (1) WO2002061139A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2400448T3 (es) * 2001-04-30 2013-04-10 Ventana Medical Systems, Inc. Reactivos y métodos para la hibridación automatizada in situ o para microarray
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
WO2008018839A1 (en) * 2006-08-09 2008-02-14 National University Of Singapore Method for molecular biology applications
US7695929B2 (en) 2006-11-01 2010-04-13 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
ES2731432T3 (es) * 2007-05-23 2019-11-15 Ventana Med Syst Inc Transportadores poliméricos para inmunohistoquímica e hibridación in situ
EP2636757B1 (en) 2008-05-27 2016-11-23 Dako Denmark A/S Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers
DK2995925T3 (da) 2008-06-05 2022-02-21 Ventana Med Syst Inc Sammensætning til histokemisk processering
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
WO2011057017A1 (en) * 2009-11-06 2011-05-12 Bend Research, Inc. Aqueous nanoparticle suspensions for use in drug discovery
SG192862A1 (en) 2011-03-11 2013-09-30 Univ Singapore Pericyte progenitors from peripheral blood
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
US10837879B2 (en) * 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
ES2753274T5 (es) * 2015-08-21 2023-05-26 42 Life Sciences Gmbh & Co Kg Composición y procedimiento de hibridación
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
ES3008982T3 (en) 2017-08-18 2025-03-25 Neutrolis Inc Engineered dnase enzymes and use in therapy
CA3109238A1 (en) 2018-08-15 2020-02-20 Illumina, Inc. Compositions and methods for improving library enrichment
EP3864147A4 (en) 2018-10-08 2022-07-06 Neutrolis, Inc. MANIPULATION OF DNASE ENZYMES FOR MANUFACTURE AND THERAPY
US11058724B2 (en) 2018-10-08 2021-07-13 Neutrolis, Inc. Methods of using DNASE1-like 3 in therapy
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
US11352613B2 (en) 2019-02-04 2022-06-07 Neutrolis, Inc. Engineered human extracellular DNASE enzymes

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4689294A (en) 1984-11-19 1987-08-25 Miles Laboratories, Inc. Enhancement of hybridization of nucleic acids by anionic polymers
US5985549A (en) 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5273905A (en) 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5750340A (en) 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6127188A (en) * 1995-10-06 2000-10-03 Mj Research, Inc. Method and apparatus for controlling evaporation in histological procedures
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
ES2354598T3 (es) * 1998-02-27 2011-03-16 Ventana Medical Systems, Inc. Aparato de patología molecular automatizado que tiene calefactores de portaobjetos independientes.
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations

Also Published As

Publication number Publication date
EP1356116A2 (en) 2003-10-29
EP1356116B1 (en) 2007-05-09
US6656685B2 (en) 2003-12-02
JP2005503762A (ja) 2005-02-10
DE60220018D1 (de) 2007-06-21
US20020102554A1 (en) 2002-08-01
ATE361995T1 (de) 2007-06-15
WO2002061139A3 (en) 2003-04-03
US20040253603A1 (en) 2004-12-16
WO2002061139A2 (en) 2002-08-08
CA2434854A1 (en) 2002-08-08
AU2002240180B2 (en) 2006-02-09

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