DE60220018T2 - Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung - Google Patents

Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Download PDF

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Publication number
DE60220018T2
DE60220018T2 DE60220018T DE60220018T DE60220018T2 DE 60220018 T2 DE60220018 T2 DE 60220018T2 DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T2 DE60220018 T2 DE 60220018T2
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Germany
Prior art keywords
dextran sulfate
hybridization
molecular weight
probe
tissue section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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DE60220018T
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German (de)
English (en)
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DE60220018D1 (de
Inventor
Joseph Tucson UTERMOHLEN
Catherine Wolf
Kimberly Tucson CHRISTENSEN
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of DE60220018D1 publication Critical patent/DE60220018D1/de
Application granted granted Critical
Publication of DE60220018T2 publication Critical patent/DE60220018T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
DE60220018T 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Expired - Fee Related DE60220018T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US772123 2001-01-29
US09/772,123 US6656685B2 (en) 2001-01-29 2001-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use
PCT/US2002/002642 WO2002061139A2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Publications (2)

Publication Number Publication Date
DE60220018D1 DE60220018D1 (de) 2007-06-21
DE60220018T2 true DE60220018T2 (de) 2008-01-10

Family

ID=25093987

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60220018T Expired - Fee Related DE60220018T2 (de) 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung

Country Status (8)

Country Link
US (2) US6656685B2 (enExample)
EP (1) EP1356116B1 (enExample)
JP (1) JP2005503762A (enExample)
AT (1) ATE361995T1 (enExample)
AU (1) AU2002240180B2 (enExample)
CA (1) CA2434854A1 (enExample)
DE (1) DE60220018T2 (enExample)
WO (1) WO2002061139A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1385999B1 (en) * 2001-04-30 2012-12-12 Ventana Medical Systems, Inc. Reagents and methods for automated in situ or microarray hybridization
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
EP2057289B1 (en) * 2006-08-09 2016-08-10 Univ Singapore PROCESS FOR MOLECULAR BIOLOGICAL APPLICATIONS
CA3069091C (en) 2006-11-01 2021-09-14 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
EP2167963B1 (en) 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
EP2636756B2 (en) 2008-05-27 2019-11-20 Dako Denmark A/S Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers
US8703490B2 (en) 2008-06-05 2014-04-22 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
EP2401396B1 (en) 2009-02-26 2016-11-23 Dako Denmark A/S Methods for performing a stringent wash step in hybridization applications
WO2011057017A1 (en) * 2009-11-06 2011-05-12 Bend Research, Inc. Aqueous nanoparticle suspensions for use in drug discovery
EP2683814A4 (en) 2011-03-11 2014-09-24 Univ Singapore PERIPHERAL BLOOD PERFECT PERFORMER
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3252173A1 (en) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridization compositions and methods
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
DK3133166T4 (da) * 2015-08-21 2023-03-20 42 Life Sciences Gmbh & Co Kg Sammensætning og fremgangsmåde til hybridisering
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
ES3008982T3 (en) 2017-08-18 2025-03-25 Neutrolis Inc Engineered dnase enzymes and use in therapy
JP7431802B2 (ja) * 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法
US11058724B2 (en) 2018-10-08 2021-07-13 Neutrolis, Inc. Methods of using DNASE1-like 3 in therapy
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
EP3864147A4 (en) 2018-10-08 2022-07-06 Neutrolis, Inc. DNASE ENZYME ENGINEERING FOR MANUFACTURING AND THERAPY
US11352613B2 (en) 2019-02-04 2022-06-07 Neutrolis, Inc. Engineered human extracellular DNASE enzymes

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4689294A (en) 1984-11-19 1987-08-25 Miles Laboratories, Inc. Enhancement of hybridization of nucleic acids by anionic polymers
US5985549A (en) 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5273905A (en) * 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5750340A (en) 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6127188A (en) * 1995-10-06 2000-10-03 Mj Research, Inc. Method and apparatus for controlling evaporation in histological procedures
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
CA2320750C (en) * 1998-02-27 2004-08-24 Ventana Medical Systems, Inc. System and method of aspirating and dispensing reagent
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations

Also Published As

Publication number Publication date
AU2002240180B2 (en) 2006-02-09
US20040253603A1 (en) 2004-12-16
CA2434854A1 (en) 2002-08-08
DE60220018D1 (de) 2007-06-21
EP1356116A2 (en) 2003-10-29
JP2005503762A (ja) 2005-02-10
US6656685B2 (en) 2003-12-02
WO2002061139A2 (en) 2002-08-08
US20020102554A1 (en) 2002-08-01
WO2002061139A3 (en) 2003-04-03
ATE361995T1 (de) 2007-06-15
EP1356116B1 (en) 2007-05-09

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