DE68928080T2 - Einstufiges in situ-hybridierungstestverfahren - Google Patents

Einstufiges in situ-hybridierungstestverfahren

Info

Publication number
DE68928080T2
DE68928080T2 DE68928080T DE68928080T DE68928080T2 DE 68928080 T2 DE68928080 T2 DE 68928080T2 DE 68928080 T DE68928080 T DE 68928080T DE 68928080 T DE68928080 T DE 68928080T DE 68928080 T2 DE68928080 T2 DE 68928080T2
Authority
DE
Germany
Prior art keywords
test procedure
hybrid test
situ hybrid
situ
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68928080T
Other languages
English (en)
Other versions
DE68928080D1 (de
Inventor
Mary Evinger-Hodges
Joel Bresser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aprogenex Inc
Original Assignee
Aprogenex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aprogenex Inc filed Critical Aprogenex Inc
Application granted granted Critical
Publication of DE68928080D1 publication Critical patent/DE68928080D1/de
Publication of DE68928080T2 publication Critical patent/DE68928080T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
DE68928080T 1988-08-31 1989-08-18 Einstufiges in situ-hybridierungstestverfahren Expired - Lifetime DE68928080T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23910688A 1988-08-31 1988-08-31
PCT/US1989/003580 WO1990002173A1 (en) 1988-08-31 1989-08-18 One-step in situ hybridization assay

Publications (2)

Publication Number Publication Date
DE68928080D1 DE68928080D1 (de) 1997-07-03
DE68928080T2 true DE68928080T2 (de) 1998-01-15

Family

ID=22900643

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68928080T Expired - Lifetime DE68928080T2 (de) 1988-08-31 1989-08-18 Einstufiges in situ-hybridierungstestverfahren

Country Status (12)

Country Link
EP (2) EP0432221B1 (de)
JP (1) JPH04502552A (de)
AT (1) ATE153705T1 (de)
AU (1) AU636854B2 (de)
DE (1) DE68928080T2 (de)
DK (1) DK34191A (de)
FI (1) FI910957A0 (de)
IL (1) IL91405A (de)
NZ (1) NZ230389A (de)
PT (1) PT91589B (de)
WO (1) WO1990002173A1 (de)
ZA (1) ZA896490B (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
AU636854B2 (en) * 1988-08-31 1993-05-13 Aprogenex, Inc. One-step in situ hybridization assay
US6242184B1 (en) 1988-10-18 2001-06-05 University Of Massachusetts In-situ hybridization of single-copy and multiple-copy nucleic acid sequences
US6203977B1 (en) 1988-11-15 2001-03-20 Yale University Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization
WO1990010715A1 (en) * 1989-03-07 1990-09-20 Molecular Biosystems, Inc. In-situ hybridization in suspension for detection or separation of cells
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
CA2140278C (en) * 1992-07-17 2009-03-17 Morteza Asgari Enriching and identyfing fetal cells in maternal blood for in situ hybridization
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
AU6631696A (en) * 1995-07-28 1997-02-26 Rijksuniversiteit Groningen Methods and materials for determining relative abundance of microorganisms in mixed populations
JP2001509662A (ja) * 1996-04-19 2001-07-24 ファルマシア・アンド・アップジョン・カンパニー 疾患特異的遺伝子の定量および発見のための高容量ｉｎ―ｓｉｔｕ ｍＲＮＡ ハイブリダイゼーション法
PT1080228E (pt) * 1998-05-18 2010-07-22 Igenex Inc MéTODO DE HIBRIDAÆO IN SITU PARA A DETECÆO DE áCIDO NUCLEICO ALVO
JP4557347B2 (ja) * 2000-01-30 2010-10-06 株式会社カイノス 特定の塩基配列を有する標的核酸の検出またはその核酸量の測定方法
US20040115692A1 (en) * 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
WO2011134474A1 (en) 2010-04-30 2011-11-03 Exiqon A/S In situ hybridization method and buffer
WO2021207062A1 (en) * 2020-04-07 2021-10-14 Advanced Cell Diagnostics, Inc. Methods for detecting target nucleic acids by in situ hybridization and a kit thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU636854B2 (en) * 1988-08-31 1993-05-13 Aprogenex, Inc. One-step in situ hybridization assay
WO1990010715A1 (en) * 1989-03-07 1990-09-20 Molecular Biosystems, Inc. In-situ hybridization in suspension for detection or separation of cells

Also Published As

Publication number Publication date
PT91589A (pt) 1990-03-08
JPH04502552A (ja) 1992-05-14
AU636854B2 (en) 1993-05-13
DK34191D0 (da) 1991-02-27
EP0357436A2 (de) 1990-03-07
NZ230389A (en) 1991-11-26
AU4343989A (en) 1990-03-23
EP0432221B1 (de) 1997-05-28
EP0432221A1 (de) 1991-06-19
DE68928080D1 (de) 1997-07-03
PT91589B (pt) 1995-08-09
EP0432221A4 (en) 1992-01-15
WO1990002173A1 (en) 1990-03-08
ZA896490B (en) 1990-06-27
ATE153705T1 (de) 1997-06-15
IL91405A0 (en) 1990-04-29
IL91405A (en) 1996-03-31
EP0357436A3 (de) 1991-06-12
DK34191A (da) 1991-04-30
FI910957A0 (fi) 1991-02-27

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Legal Events

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