ES8609472A1 - Una sonda de deteccion de acidos nucleicos - Google Patents

Una sonda de deteccion de acidos nucleicos

Info

Publication number
ES8609472A1
ES8609472A1 ES538291A ES538291A ES8609472A1 ES 8609472 A1 ES8609472 A1 ES 8609472A1 ES 538291 A ES538291 A ES 538291A ES 538291 A ES538291 A ES 538291A ES 8609472 A1 ES8609472 A1 ES 8609472A1
Authority
ES
Spain
Prior art keywords
hybridizable
nucleic acid
detection
acid hybridization
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES538291A
Other languages
English (en)
Other versions
ES538291A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Diagnostics Inc
Original Assignee
Molecular Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Diagnostics Inc filed Critical Molecular Diagnostics Inc
Publication of ES538291A0 publication Critical patent/ES538291A0/es
Publication of ES8609472A1 publication Critical patent/ES8609472A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO DE FABRICACION DE UNA SONDA DE DETECCION DE ACIDOS NUCLEICOS. CONSISTENTE EN CONECTAR, POR MEDIO DE UNA REACCION ENZIMATICA O QUIMICA: A) UN FRAGMENTO MONOCATENARIO HIBRIDABLE DE UN ACIDO NUCLEICO QUE ES COMPLEMENTARIO A LA SECUENCIA DEL ACIDO NUCLEICO A DETECTAR Y B) UN FRAGMENTO MONOCATENARIO O BICATENARIO, DE ACIDO NUCLEICO NO HIBRIDABLE, CUYO FRAGMENTO DE ACIDO NUCLEICO NO HIBRIDABLE COMPRENDE SITIO DE RECONOCIMIENTO PARA UNA PROTEINA DETERMINADA O ESTA MARCADO CON UN TRAZADOR DETECTABLE. LA PROTEINA ES SELECCIONADA ENTRE EL GRUPO FORMADO POR REPRESOR DE LACTOSA, REPRESOR DE GALACTOSA, REPRESOR LAMBDA, PROTEINA ACTIVANTE DEL GEN CATABOLITICO Y PROTEINA CRO. SE USA CON FINES ANALITICOS Y DE DIAGNOSTICO, PARA DETECTAR SECUENCIAS DE POLINUCLEOTIDOS ESPECIFICOS.
ES538291A 1983-12-12 1984-12-05 Una sonda de deteccion de acidos nucleicos Expired ES8609472A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/560,462 US4724202A (en) 1983-12-12 1983-12-12 Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization

Publications (2)

Publication Number Publication Date
ES538291A0 ES538291A0 (es) 1986-07-16
ES8609472A1 true ES8609472A1 (es) 1986-07-16

Family

ID=24237928

Family Applications (1)

Application Number Title Priority Date Filing Date
ES538291A Expired ES8609472A1 (es) 1983-12-12 1984-12-05 Una sonda de deteccion de acidos nucleicos

Country Status (5)

Country Link
US (1) US4724202A (es)
JP (1) JPS60144662A (es)
CA (1) CA1266434A (es)
ES (1) ES8609472A1 (es)
ZA (1) ZA849622B (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5221609A (en) * 1983-05-31 1993-06-22 Orgenics Ltd. Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
US5366603A (en) * 1984-03-29 1994-11-22 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US6221581B1 (en) * 1984-04-27 2001-04-24 Enzo Diagnostics, Inc. Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions
JPS60256059A (ja) * 1984-06-01 1985-12-17 Fujirebio Inc ポリヌクレオチドの測定法
US4760017A (en) * 1985-12-23 1988-07-26 E. I. Du Pont De Nemours And Company Arabinonucleic acid probes for DNA/RNA assays
US4968602A (en) * 1986-03-05 1990-11-06 Molecular Diagnostics, Inc. Solution-phase single hybridization assay for detecting polynucleotide sequences
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
GB2190189B (en) * 1986-03-21 1990-06-13 Block Myron Jacques Assay for polynucleotides
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
WO1988010313A1 (en) * 1987-06-26 1988-12-29 E.I. Du Pont De Nemours And Company Affinity removal of contaminating sequences from recombinant cloned na using capture beads
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
EP0453301A3 (en) * 1990-04-20 1993-07-21 Syntex (U.S.A.) Inc. Double receptor polynucleotide assay method
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
DK0549107T3 (da) 1991-10-11 1998-08-31 Dade Behring Marburg Gmbh Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli
US5556961A (en) * 1991-11-15 1996-09-17 Foote; Robert S. Nucleosides with 5'-O-photolabile protecting groups
EP0652973B1 (en) * 1992-07-31 1997-04-23 BEHRINGWERKE Aktiengesellschaft Method for introducing defined sequences at the 3' end of polynucleotides
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
US6294323B1 (en) 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
US5744306A (en) * 1993-04-19 1998-04-28 Emory University Methods for nucleic acid detection, sequencing, and cloning using exonuclease
WO1995018236A1 (fr) * 1993-12-28 1995-07-06 Daikin Industries, Ltd. PROCEDE D'HYBRIDATION IN SITU AU MOYEN D'UNE PROTEINE RecA, ET PROTEINE RecA COMPRENANT UN MARQUEUR OU UN LIGAND UTILISEE POUR METTRE EN ×UVRE LEDIT PROCEDE
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US20030104361A1 (en) * 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US6287765B1 (en) * 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
AU782254B2 (en) * 1999-05-05 2005-07-14 Regents Of The University Of California, The Method for detecting biological agents
US6541671B1 (en) * 2002-02-13 2003-04-01 The Regents Of The University Of California Synthesis of 2H- and 13C-substituted dithanes
US7205146B1 (en) * 2000-06-14 2007-04-17 Oscient Pharmaceuticals Corporation Nucleotide and amino acid sequences relating to respiratory diseases and obesity
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2005087932A2 (en) * 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4395486A (en) * 1981-08-19 1983-07-26 Medical College Of Ga. Research Inst., Inc. Method for the direct analysis of sickle cell anemia
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes

Also Published As

Publication number Publication date
ES538291A0 (es) 1986-07-16
US4724202A (en) 1988-02-09
ZA849622B (en) 1985-08-28
JPS60144662A (ja) 1985-07-31
CA1266434A (en) 1990-03-06

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