AU2002240180B2 - Hybridization buffers using low molecular weight dextran sulfate and methods for their use - Google Patents

Hybridization buffers using low molecular weight dextran sulfate and methods for their use Download PDF

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Publication number
AU2002240180B2
AU2002240180B2 AU2002240180A AU2002240180A AU2002240180B2 AU 2002240180 B2 AU2002240180 B2 AU 2002240180B2 AU 2002240180 A AU2002240180 A AU 2002240180A AU 2002240180 A AU2002240180 A AU 2002240180A AU 2002240180 B2 AU2002240180 B2 AU 2002240180B2
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AU
Australia
Prior art keywords
hybridization
dextran sulfate
molecular weight
buffer
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2002240180A
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English (en)
Other versions
AU2002240180A1 (en
Inventor
Kimberly Christensen
Joseph Utermohlen
Catherine Wolf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of AU2002240180A1 publication Critical patent/AU2002240180A1/en
Application granted granted Critical
Publication of AU2002240180B2 publication Critical patent/AU2002240180B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
AU2002240180A 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use Ceased AU2002240180B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/772,123 2001-01-29
US09/772,123 US6656685B2 (en) 2001-01-29 2001-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use
PCT/US2002/002642 WO2002061139A2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Publications (2)

Publication Number Publication Date
AU2002240180A1 AU2002240180A1 (en) 2003-02-20
AU2002240180B2 true AU2002240180B2 (en) 2006-02-09

Family

ID=25093987

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002240180A Ceased AU2002240180B2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Country Status (8)

Country Link
US (2) US6656685B2 (enExample)
EP (1) EP1356116B1 (enExample)
JP (1) JP2005503762A (enExample)
AT (1) ATE361995T1 (enExample)
AU (1) AU2002240180B2 (enExample)
CA (1) CA2434854A1 (enExample)
DE (1) DE60220018T2 (enExample)
WO (1) WO2002061139A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1385999B1 (en) * 2001-04-30 2012-12-12 Ventana Medical Systems, Inc. Reagents and methods for automated in situ or microarray hybridization
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
WO2008018839A1 (en) * 2006-08-09 2008-02-14 National University Of Singapore Method for molecular biology applications
EP3029461B1 (en) 2006-11-01 2017-12-20 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
JP2010528285A (ja) 2007-05-23 2010-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学およびｉｎｓｉｔｕハイブリダーゼーションのためのポリマー担体
BRPI0912046B1 (pt) 2008-05-27 2020-11-24 Agilent Technologies, Inc Composições de hibridizaqao e seu uso, bem como metodo de hibridizaqao de sequencias de acido nucleico
DK2300799T3 (en) 2008-06-05 2015-12-14 Ventana Med Syst Inc Process for the histo chemical processing and use of a composition for histo chemical processing
EP2401396B1 (en) 2009-02-26 2016-11-23 Dako Denmark A/S Methods for performing a stringent wash step in hybridization applications
WO2011057017A1 (en) * 2009-11-06 2011-05-12 Bend Research, Inc. Aqueous nanoparticle suspensions for use in drug discovery
SG10201601853XA (en) 2011-03-11 2016-04-28 Univ Singapore Pericyte progenitors from peripheral blood
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3252173A1 (en) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridization compositions and methods
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
ES2753274T5 (es) * 2015-08-21 2023-05-26 42 Life Sciences Gmbh & Co Kg Composición y procedimiento de hibridación
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
SG11202001445XA (en) 2017-08-18 2020-03-30 Neutrolis Inc Engineered dnase enzymes and use in therapy
AU2019320771B2 (en) * 2018-08-15 2025-12-18 Illumina Cambridge Limited Compositions and methods for improving library enrichment
US11058724B2 (en) 2018-10-08 2021-07-13 Neutrolis, Inc. Methods of using DNASE1-like 3 in therapy
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
SG11202103426XA (en) 2018-10-08 2021-05-28 Neutrolis Inc Engineering of dnase enzymes for manufacturing and therapy
US11352613B2 (en) 2019-02-04 2022-06-07 Neutrolis, Inc. Engineered human extracellular DNASE enzymes

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4689294A (en) 1984-11-19 1987-08-25 Miles Laboratories, Inc. Enhancement of hybridization of nucleic acids by anionic polymers
US5985549A (en) 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5273905A (en) * 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5750340A (en) 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6127188A (en) * 1995-10-06 2000-10-03 Mj Research, Inc. Method and apparatus for controlling evaporation in histological procedures
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
WO1999043434A1 (en) * 1998-02-27 1999-09-02 Ventana Medical Systems, Inc. System and method of aspirating and dispensing reagent
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations

Also Published As

Publication number Publication date
US20040253603A1 (en) 2004-12-16
DE60220018T2 (de) 2008-01-10
WO2002061139A2 (en) 2002-08-08
US6656685B2 (en) 2003-12-02
CA2434854A1 (en) 2002-08-08
JP2005503762A (ja) 2005-02-10
EP1356116A2 (en) 2003-10-29
US20020102554A1 (en) 2002-08-01
WO2002061139A3 (en) 2003-04-03
DE60220018D1 (de) 2007-06-21
EP1356116B1 (en) 2007-05-09
ATE361995T1 (de) 2007-06-15

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired