CA2434854A1 - Hybridization buffers using low molecular weight dextran sulfate and methods for their use - Google Patents

Hybridization buffers using low molecular weight dextran sulfate and methods for their use Download PDF

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Publication number
CA2434854A1
CA2434854A1 CA002434854A CA2434854A CA2434854A1 CA 2434854 A1 CA2434854 A1 CA 2434854A1 CA 002434854 A CA002434854 A CA 002434854A CA 2434854 A CA2434854 A CA 2434854A CA 2434854 A1 CA2434854 A1 CA 2434854A1
Authority
CA
Canada
Prior art keywords
dextran sulfate
hybridization
molecular weight
vol
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002434854A
Other languages
English (en)
French (fr)
Inventor
Joseph Utermohlen
Catherine Wolf
Kimberly Christensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2434854A1 publication Critical patent/CA2434854A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
CA002434854A 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use Abandoned CA2434854A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/772,123 2001-01-29
US09/772,123 US6656685B2 (en) 2001-01-29 2001-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use
PCT/US2002/002642 WO2002061139A2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Publications (1)

Publication Number Publication Date
CA2434854A1 true CA2434854A1 (en) 2002-08-08

Family

ID=25093987

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002434854A Abandoned CA2434854A1 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Country Status (8)

Country Link
US (2) US6656685B2 (enExample)
EP (1) EP1356116B1 (enExample)
JP (1) JP2005503762A (enExample)
AT (1) ATE361995T1 (enExample)
AU (1) AU2002240180B2 (enExample)
CA (1) CA2434854A1 (enExample)
DE (1) DE60220018T2 (enExample)
WO (1) WO2002061139A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1385999B1 (en) * 2001-04-30 2012-12-12 Ventana Medical Systems, Inc. Reagents and methods for automated in situ or microarray hybridization
WO2005033333A2 (en) * 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
WO2008018839A1 (en) * 2006-08-09 2008-02-14 National University Of Singapore Method for molecular biology applications
EP3029461B1 (en) 2006-11-01 2017-12-20 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
JP2010528285A (ja) 2007-05-23 2010-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学およびｉｎｓｉｔｕハイブリダーゼーションのためのポリマー担体
BRPI0912046B1 (pt) 2008-05-27 2020-11-24 Agilent Technologies, Inc Composições de hibridizaqao e seu uso, bem como metodo de hibridizaqao de sequencias de acido nucleico
DK2300799T3 (en) 2008-06-05 2015-12-14 Ventana Med Syst Inc Process for the histo chemical processing and use of a composition for histo chemical processing
EP2401396B1 (en) 2009-02-26 2016-11-23 Dako Denmark A/S Methods for performing a stringent wash step in hybridization applications
WO2011057017A1 (en) * 2009-11-06 2011-05-12 Bend Research, Inc. Aqueous nanoparticle suspensions for use in drug discovery
SG10201601853XA (en) 2011-03-11 2016-04-28 Univ Singapore Pericyte progenitors from peripheral blood
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3252173A1 (en) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridization compositions and methods
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
ES2753274T5 (es) * 2015-08-21 2023-05-26 42 Life Sciences Gmbh & Co Kg Composición y procedimiento de hibridación
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
SG11202001445XA (en) 2017-08-18 2020-03-30 Neutrolis Inc Engineered dnase enzymes and use in therapy
AU2019320771B2 (en) * 2018-08-15 2025-12-18 Illumina Cambridge Limited Compositions and methods for improving library enrichment
US11058724B2 (en) 2018-10-08 2021-07-13 Neutrolis, Inc. Methods of using DNASE1-like 3 in therapy
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
SG11202103426XA (en) 2018-10-08 2021-05-28 Neutrolis Inc Engineering of dnase enzymes for manufacturing and therapy
US11352613B2 (en) 2019-02-04 2022-06-07 Neutrolis, Inc. Engineered human extracellular DNASE enzymes

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4689294A (en) 1984-11-19 1987-08-25 Miles Laboratories, Inc. Enhancement of hybridization of nucleic acids by anionic polymers
US5985549A (en) 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5273905A (en) * 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5750340A (en) 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6127188A (en) * 1995-10-06 2000-10-03 Mj Research, Inc. Method and apparatus for controlling evaporation in histological procedures
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
WO1999043434A1 (en) * 1998-02-27 1999-09-02 Ventana Medical Systems, Inc. System and method of aspirating and dispensing reagent
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations

Also Published As

Publication number Publication date
US20040253603A1 (en) 2004-12-16
DE60220018T2 (de) 2008-01-10
WO2002061139A2 (en) 2002-08-08
US6656685B2 (en) 2003-12-02
JP2005503762A (ja) 2005-02-10
EP1356116A2 (en) 2003-10-29
US20020102554A1 (en) 2002-08-01
WO2002061139A3 (en) 2003-04-03
DE60220018D1 (de) 2007-06-21
AU2002240180B2 (en) 2006-02-09
EP1356116B1 (en) 2007-05-09
ATE361995T1 (de) 2007-06-15

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued