DE602004024529D1 - Verfahren zur herstellung eines retinalen neurozyten aus einer von irisgewebe abgeleiteten stammzelle sowie mit dem verfahren hergestellter retinaler neurozyt - Google Patents

Verfahren zur herstellung eines retinalen neurozyten aus einer von irisgewebe abgeleiteten stammzelle sowie mit dem verfahren hergestellter retinaler neurozyt

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Publication number
DE602004024529D1
DE602004024529D1 DE602004024529T DE602004024529T DE602004024529D1 DE 602004024529 D1 DE602004024529 D1 DE 602004024529D1 DE 602004024529 T DE602004024529 T DE 602004024529T DE 602004024529 T DE602004024529 T DE 602004024529T DE 602004024529 D1 DE602004024529 D1 DE 602004024529D1
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DE
Germany
Prior art keywords
retinal
cells
neurocyte
pigmented epithelial
produced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE602004024529T
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English (en)
Inventor
Mitsuko Kosaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
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Japan Science and Technology Agency
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Application filed by Japan Science and Technology Agency filed Critical Japan Science and Technology Agency
Publication of DE602004024529D1 publication Critical patent/DE602004024529D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Neurology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Ophthalmology & Optometry (AREA)
  • Physics & Mathematics (AREA)
  • Acoustics & Sound (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
DE602004024529T 2003-06-11 2004-06-11 Verfahren zur herstellung eines retinalen neurozyten aus einer von irisgewebe abgeleiteten stammzelle sowie mit dem verfahren hergestellter retinaler neurozyt Expired - Lifetime DE602004024529D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003166646 2003-06-11
PCT/JP2004/008222 WO2004111213A1 (ja) 2003-06-11 2004-06-11 虹彩組織由来の神経幹細胞から網膜神経細胞を生産する方法、および、その方法により得られる網膜神経細胞

Publications (1)

Publication Number Publication Date
DE602004024529D1 true DE602004024529D1 (de) 2010-01-21

Family

ID=33549263

Family Applications (2)

Application Number Title Priority Date Filing Date
DE602004024529T Expired - Lifetime DE602004024529D1 (de) 2003-06-11 2004-06-11 Verfahren zur herstellung eines retinalen neurozyten aus einer von irisgewebe abgeleiteten stammzelle sowie mit dem verfahren hergestellter retinaler neurozyt
DE602004028768T Expired - Lifetime DE602004028768D1 (de) 2003-06-11 2004-06-11 Verfahren zur Herstellung von Netzhautneurozyten aus neutralen Stammzellen aus dem Irisgewebe und in dem Verfahren hergestelltes Netzhautneurozyt

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE602004028768T Expired - Lifetime DE602004028768D1 (de) 2003-06-11 2004-06-11 Verfahren zur Herstellung von Netzhautneurozyten aus neutralen Stammzellen aus dem Irisgewebe und in dem Verfahren hergestelltes Netzhautneurozyt

Country Status (12)

Country Link
US (1) US8569056B2 (de)
EP (2) EP1988160B1 (de)
JP (2) JP4389087B2 (de)
KR (1) KR100734714B1 (de)
CN (2) CN1795266B (de)
AT (2) ATE478136T1 (de)
AU (1) AU2004248013B2 (de)
BR (1) BRPI0411236A (de)
CA (2) CA2528426C (de)
DE (2) DE602004024529D1 (de)
IL (1) IL172499A (de)
WO (1) WO2004111213A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124276A1 (en) * 2006-07-24 2008-05-29 Lifeline Cell Technology Synthetic cornea from retinal stem cells
JP2013135640A (ja) * 2011-12-28 2013-07-11 Shiseido Co Ltd 神経幹細胞から神経細胞及び／又はグリア細胞を形成する培養方法
JP6050033B2 (ja) * 2012-06-05 2016-12-21 日本メナード化粧品株式会社 幹細胞から外胚葉系細胞への分化誘導剤
US9597227B2 (en) 2013-03-15 2017-03-21 Abbott Medical Optics Inc. Trans-sclera portal for delivery of therapeutic agents
EP2796545A1 (de) * 2013-04-26 2014-10-29 Université Pierre et Marie Curie (Paris 6) Verfahren zur Herstellung von Retinavorläufern, pigmentierten Retinaepithelzellen und neuronalen Retinazellen
CN103409363B (zh) * 2013-08-06 2015-01-21 中国人民解放军总医院 感光前体细胞与视网膜组织体外共培养方法
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
KR101895648B1 (ko) 2014-11-06 2018-09-05 단국대학교 천안캠퍼스 산학협력단 치수줄기세포를 포함하는 신경질환 치료용 약학 조성물 및 이의 제조방법
CN113943707B (zh) * 2021-09-24 2023-11-21 中国人民解放军陆军军医大学第一附属医院 一种视网膜前体细胞的筛选和培养方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60142553D1 (de) * 2000-02-11 2010-08-26 Children S Hospital Of Orange Isolierung und transplantation von retinalen stammzellen
JP2002112764A (ja) 2000-10-05 2002-04-16 Tohoku Techno Arch Co Ltd 株化網膜神経細胞
JP2002325571A (ja) 2001-04-27 2002-11-12 Purotekku:Kk 網膜の分化誘導方法
JP3723152B2 (ja) * 2002-05-10 2005-12-07 独立行政法人科学技術振興機構 哺乳動物の虹彩色素上皮細胞由来の神経幹細胞の生産方法、および該神経幹細胞から神経系細胞を生産する方法

Also Published As

Publication number Publication date
EP1988160A1 (de) 2008-11-05
EP1640450A1 (de) 2006-03-29
AU2004248013B2 (en) 2007-02-08
KR20060017543A (ko) 2006-02-23
US8569056B2 (en) 2013-10-29
JP2009273473A (ja) 2009-11-26
ATE478136T1 (de) 2010-09-15
EP1640450A4 (de) 2006-09-06
ATE451449T1 (de) 2009-12-15
CN101186900A (zh) 2008-05-28
DE602004028768D1 (de) 2010-09-30
JP4389087B2 (ja) 2009-12-24
CA2717252C (en) 2013-09-24
CA2528426C (en) 2012-04-03
WO2004111213A1 (ja) 2004-12-23
EP1988160B1 (de) 2010-08-18
CN101186900B (zh) 2010-09-15
KR100734714B1 (ko) 2007-07-02
AU2004248013A1 (en) 2004-12-23
CA2717252A1 (en) 2004-12-23
IL172499A0 (en) 2006-04-10
EP1640450B1 (de) 2009-12-09
CA2528426A1 (en) 2004-12-23
US20060134280A1 (en) 2006-06-22
BRPI0411236A (pt) 2006-07-11
IL172499A (en) 2011-09-27
CN1795266A (zh) 2006-06-28
CN1795266B (zh) 2010-08-18
JPWO2004111213A1 (ja) 2006-07-27

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