DE60033825T2 - Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration - Google Patents

Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration Download PDF

Info

Publication number
DE60033825T2
DE60033825T2 DE60033825T DE60033825T DE60033825T2 DE 60033825 T2 DE60033825 T2 DE 60033825T2 DE 60033825 T DE60033825 T DE 60033825T DE 60033825 T DE60033825 T DE 60033825T DE 60033825 T2 DE60033825 T2 DE 60033825T2
Authority
DE
Germany
Prior art keywords
primer
sequence
target
nucleic acid
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60033825T
Other languages
German (de)
English (en)
Other versions
DE60033825D1 (de
Inventor
Alla San Jose LISHANSKI
Marc Mountain View TAYLOR
Nurith Palo Alto KURN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Dade Behring Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Inc filed Critical Dade Behring Inc
Application granted granted Critical
Publication of DE60033825D1 publication Critical patent/DE60033825D1/de
Publication of DE60033825T2 publication Critical patent/DE60033825T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60033825T 1999-08-17 2000-08-15 Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration Expired - Lifetime DE60033825T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US376097 1982-05-07
US09/376,097 US6232104B1 (en) 1999-08-17 1999-08-17 Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
PCT/US2000/022446 WO2001012859A2 (en) 1999-08-17 2000-08-15 Detection of differences in nucleic acids by inhibition of spontaneous dna branch migration

Publications (2)

Publication Number Publication Date
DE60033825D1 DE60033825D1 (de) 2007-04-19
DE60033825T2 true DE60033825T2 (de) 2007-11-08

Family

ID=23483699

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60033825T Expired - Lifetime DE60033825T2 (de) 1999-08-17 2000-08-15 Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration

Country Status (6)

Country Link
US (1) US6232104B1 (https=)
EP (1) EP1210457B1 (https=)
JP (1) JP2003507025A (https=)
DE (1) DE60033825T2 (https=)
ES (1) ES2281354T3 (https=)
WO (1) WO2001012859A2 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001084157A2 (en) * 2000-05-04 2001-11-08 Dade Behring Marburg Gmbh Compositions for detection of multiple analytes
JP4982921B2 (ja) * 2001-03-05 2012-07-25 ルネサスエレクトロニクス株式会社 半導体装置及びその製造方法
US6878530B2 (en) * 2002-02-07 2005-04-12 Panomics, Inc. Methods for detecting polymorphisms in nucleic acids
GB0310270D0 (en) * 2003-05-03 2003-06-11 Univ Edinburgh Biomolecular devices
US7273762B2 (en) 2004-11-09 2007-09-25 Freescale Semiconductor, Inc. Microelectromechanical (MEM) device including a spring release bridge and method of making the same
GB0518867D0 (en) * 2005-09-15 2005-10-26 Secretary Trade Ind Brit Microscopy tip
US20140302486A1 (en) * 2011-09-02 2014-10-09 President And Fellows Of Harvard College Systems and methods for detecting biomarkers of interest
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
EP4372084A3 (en) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4026912A1 (en) 2012-05-10 2022-07-13 The General Hospital Corporation Methods for determining a nucleotide sequence
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2738263A1 (en) * 2012-11-30 2014-06-04 Fundacion para la Investigacion del Hospital Clinico de la Comunidad Valenciana Methods for DNA rearrengements analysis
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
EP3287528A1 (de) 2016-08-25 2018-02-28 AGCT GmbH Verfahren zur amplifikation von nukleinsäuren und kit zu dessen durchführung
WO2018053362A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
JP7549587B2 (ja) * 2019-02-04 2024-09-11 アコヤ・バイオサイエンシズ・インコーポレイテッド 生物学的試料の選択的標識による分析物検出
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1254114A (en) 1984-05-07 1989-05-16 Allied Corporation Displacement polynucleotide assay employing recombination protein and diagnostic kit
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
US5622820A (en) 1988-03-10 1997-04-22 City Of Hope Method for amplification and detection of RNA and DNA sequences
WO1990002819A1 (en) 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Replicative rna-based amplification detection systems
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5766849A (en) 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US5187066A (en) 1990-02-14 1993-02-16 Syntex (U.S.A.) Inc. Methods for detecting amphiphilic antigens
CA2037349C (en) 1990-03-26 2008-06-17 James G. Wetmur Branch migration of nucleotides
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5525462A (en) 1991-05-02 1996-06-11 Toyo Boseki Kabushiki Kaisha Nucleic acid sequence amplification method, detection method, and reagent kit therefor
ES2091976T3 (es) * 1991-06-20 1996-11-16 Hoffmann La Roche Metodos perfeccionados para la amplificacion del acido nucleico.
US5582981A (en) 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
EP0543484B1 (en) 1991-08-30 2001-01-31 Research Development Corporation of Japan A method of DNA amplification
DE4213029A1 (de) 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
ATE193558T1 (de) 1991-11-15 2000-06-15 Igen Int Inc Schnelle bestimmungsverfahren für amplifikationsprodukte
US5567583A (en) 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
WO1993017127A1 (en) 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
FR2690691B1 (fr) 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
JP4137996B2 (ja) 1992-05-06 2008-08-20 ジェン−プローブ・インコーポレイテッド 核酸配列増幅方法，組成物及びキット
ATE162892T1 (de) 1992-07-31 1998-02-15 Behringwerke Ag Photoaktivierbare chemiluminizierende matrices
US5340728A (en) 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5616464A (en) 1994-12-27 1997-04-01 Naxcor Nucleic acid sequence detection employing amplification probes
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
WO1995003430A1 (en) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
AU7551594A (en) 1993-07-29 1995-02-28 MURASHIGE, Kate H. Method for recognition of the nucleotide sequence of a purified dna segment
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5480783A (en) 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
US5580730A (en) 1994-08-19 1996-12-03 Olympus America, Inc. Enzyme digestion method for the detection of amplified DNA
FR2724934B1 (fr) 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5512441A (en) 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5681705A (en) 1995-08-28 1997-10-28 Schram; James L. Amplification and detection of mycobacterium avium complex species
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
AU1565397A (en) * 1995-12-22 1997-07-17 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
WO2000043546A2 (en) * 1999-01-19 2000-07-27 Dade Behring Inc. Detection of drug resistant organisms

Also Published As

Publication number Publication date
WO2001012859A2 (en) 2001-02-22
EP1210457B1 (en) 2007-03-07
WO2001012859A3 (en) 2002-01-31
EP1210457A2 (en) 2002-06-05
US6232104B1 (en) 2001-05-15
DE60033825D1 (de) 2007-04-19
JP2003507025A (ja) 2003-02-25
ES2281354T3 (es) 2007-10-01

Similar Documents

Publication Publication Date Title
DE60033825T2 (de) Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration
DE69633941T2 (de) Nachweis von unterschieden in nukleinsäuren
DE69707284T2 (de) Verfahren zur amplifizierung von polynukleotiden
DE69122457T2 (de) Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation
DE69930765T2 (de) Methode zur kontrollierten verlängerung eines oligonukleotids
DE69333242T2 (de) Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
DE69935314T2 (de) Quantitatitative bestimmung von nucleinsäure-amplifizierungsprodukten
DE69635612T2 (de) Detektion und amplifikation von nukleinsäuren durch chemische verbindung von oligonukleotiden
DE69512003T2 (de) Oligonukleotid verwendbar als Primer in einem Amplifikationsverfahren auf der Basis einer Strangverdrängungsreplikation
DE69327326T2 (de) Vervielfältigungs- und detektionsprozess
DE69329641T2 (de) Verfahren zur mehrfachen ligase-kettenreaktion
DE3885422T2 (de) Verfahren zum Nachweis einer gezielten Nukleinsäure-Sequenz.
DE69028325T2 (de) Nukleinsäure-Amplifikation unter Verwendung eines Einzelprimers
DE69429626T2 (de) Oligonukleotide und Verfahren zum Nachweis von Chlamydia trachomatis
DE69223755T2 (de) Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung
DE68923603T2 (de) Nukleotid Sonden.
EP0530794A1 (de) Verfahren zum Nachweis ähnlicher Nukleinsäuren
DE69029740T2 (de) Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe
WO1999023249A2 (de) Spezifisches und empfindliches nukleinsäurenachweisverfahren
DE68923608T2 (de) Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements.
EP0813610A1 (de) Sequenzspezifischer nachweis von nukleinsäuren
DE69734904T2 (de) ABO-Glycosyltransferase-Sequenz-Polymorphismus
DE69921594T2 (de) Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomeren
DE69329824T2 (de) Nachweis und amplifikation bestimmter nukleinsäuren mittels exonukleolytischer aktivität
EP0552203B1 (de) Verfahren zum empfindlichen nachweis von nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC., DEERFIELD, US

8328 Change in the person/name/address of the agent

Representative=s name: PLATE SCHWEITZER ZOUNEK PATENTANWAELTE, 65203 WIES