DE3739347A1 - Verfahren zur selektiven spaltung von fusionsproteinen - Google Patents

Verfahren zur selektiven spaltung von fusionsproteinen

Info

Publication number
DE3739347A1
DE3739347A1 DE19873739347 DE3739347A DE3739347A1 DE 3739347 A1 DE3739347 A1 DE 3739347A1 DE 19873739347 DE19873739347 DE 19873739347 DE 3739347 A DE3739347 A DE 3739347A DE 3739347 A1 DE3739347 A1 DE 3739347A1
Authority
DE
Germany
Prior art keywords
protein
fusion protein
gly
polypeptide
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873739347
Other languages
German (de)
English (en)
Inventor
Michael Dr Doerschug
Gerhard Dr Seipke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DE19873739347 priority Critical patent/DE3739347A1/de
Priority to AT88118673T priority patent/ATE78873T1/de
Priority to EP88118673A priority patent/EP0316748B1/de
Priority to DE8888118673T priority patent/DE3873273D1/de
Priority to ES88118673T priority patent/ES2051816T3/es
Priority to FI885328A priority patent/FI885328L/fi
Priority to PT89019A priority patent/PT89019B/pt
Priority to JP63290349A priority patent/JPH01160496A/ja
Priority to KR1019880015202A priority patent/KR890008166A/ko
Priority to CA000583545A priority patent/CA1337283C/en
Priority to IE346288A priority patent/IE62228B1/en
Priority to NZ226998A priority patent/NZ226998A/en
Priority to ZA888650A priority patent/ZA888650B/xx
Priority to NO885154A priority patent/NO173194C/no
Priority to AU25695/88A priority patent/AU618035B2/en
Priority to IL88413A priority patent/IL88413A/xx
Priority to DK645588A priority patent/DK645588A/da
Priority to HU885958A priority patent/HU204894B/hu
Priority to CN88107955A priority patent/CN1033184A/zh
Publication of DE3739347A1 publication Critical patent/DE3739347A1/de
Priority to US07/905,482 priority patent/US5270176A/en
Priority to GR920402186T priority patent/GR3006045T3/el
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Techniques For Improving Reliability Of Storages (AREA)
  • Fuses (AREA)
  • Welding Or Cutting Using Electron Beams (AREA)
  • Exhaust Gas After Treatment (AREA)
  • Machines For Manufacturing Corrugated Board In Mechanical Paper-Making Processes (AREA)
DE19873739347 1987-11-20 1987-11-20 Verfahren zur selektiven spaltung von fusionsproteinen Withdrawn DE3739347A1 (de)

Priority Applications (21)

Application Number Priority Date Filing Date Title
DE19873739347 DE3739347A1 (de) 1987-11-20 1987-11-20 Verfahren zur selektiven spaltung von fusionsproteinen
AT88118673T ATE78873T1 (de) 1987-11-20 1988-11-10 Verfahren zur selektiven spaltung von fusionsproteinen.
EP88118673A EP0316748B1 (de) 1987-11-20 1988-11-10 Verfahren zur selektiven Spaltung von Fusionsproteinen
DE8888118673T DE3873273D1 (de) 1987-11-20 1988-11-10 Verfahren zur selektiven spaltung von fusionsproteinen.
ES88118673T ES2051816T3 (es) 1987-11-20 1988-11-10 Procedimiento para la disociacion selectiva de proteinas de fusion.
FI885328A FI885328L (fi) 1987-11-20 1988-11-17 Foerfarande foer selektiv spaltning av fusionsproteiner.
PT89019A PT89019B (pt) 1987-11-20 1988-11-17 Processo para a cisao selectiva de proteinas de fusao
NZ226998A NZ226998A (en) 1987-11-20 1988-11-18 Preparation of a peptide or protein by enzymatic hydrolysis of fusion protein
AU25695/88A AU618035B2 (en) 1987-11-20 1988-11-18 A method for the selective cleavage of fusion proteins
CA000583545A CA1337283C (en) 1987-11-20 1988-11-18 Method for the selective cleavage of fusion proteins
IE346288A IE62228B1 (en) 1987-11-20 1988-11-18 A method for the selective cleavage of fusion proteins
JP63290349A JPH01160496A (ja) 1987-11-20 1988-11-18 融合タンパク質の選択的分解方法
ZA888650A ZA888650B (en) 1987-11-20 1988-11-18 A method for the selective cleavage of fusion proteins
NO885154A NO173194C (no) 1987-11-20 1988-11-18 Fremgangsmaate for fremstilling av proinsulin
KR1019880015202A KR890008166A (ko) 1987-11-20 1988-11-18 융합 단백질의 선택적 분해 방법
IL88413A IL88413A (en) 1987-11-20 1988-11-18 Method for the selective cleavage of fusion proteins
DK645588A DK645588A (da) 1987-11-20 1988-11-18 Fremgangsmaade til fremstilling af et polypeptid eller protein ved enzymatisk spaltning
HU885958A HU204894B (en) 1987-11-20 1988-11-18 Process for producing polypeptides by enzymatic splitting of fusion proteins
CN88107955A CN1033184A (zh) 1987-11-20 1988-11-19 一种选择性切割融合蛋白质的方法
US07/905,482 US5270176A (en) 1987-11-20 1992-06-29 Method for the selective cleavage of fusion proteins with lysostaphin
GR920402186T GR3006045T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1987-11-20 1992-10-22

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873739347 DE3739347A1 (de) 1987-11-20 1987-11-20 Verfahren zur selektiven spaltung von fusionsproteinen

Publications (1)

Publication Number Publication Date
DE3739347A1 true DE3739347A1 (de) 1989-06-01

Family

ID=6340882

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19873739347 Withdrawn DE3739347A1 (de) 1987-11-20 1987-11-20 Verfahren zur selektiven spaltung von fusionsproteinen
DE8888118673T Expired - Lifetime DE3873273D1 (de) 1987-11-20 1988-11-10 Verfahren zur selektiven spaltung von fusionsproteinen.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8888118673T Expired - Lifetime DE3873273D1 (de) 1987-11-20 1988-11-10 Verfahren zur selektiven spaltung von fusionsproteinen.

Country Status (19)

Country Link
EP (1) EP0316748B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPH01160496A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR890008166A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN1033184A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AT (1) ATE78873T1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU618035B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA1337283C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (2) DE3739347A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DK (1) DK645588A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES2051816T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
FI (1) FI885328L (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
GR (1) GR3006045T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HU (1) HU204894B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IE (1) IE62228B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IL (1) IL88413A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NO (1) NO173194C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ226998A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PT (1) PT89019B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ZA (1) ZA888650B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221837B1 (en) 1996-07-26 2001-04-24 Aventis Pharma Deutschland Gmbh Insulin derivatives with increased zinc binding
US6686177B1 (en) 1998-06-06 2004-02-03 Aventis Pharma Deutschland Gmbh Insulin analogs with enhanced zinc binding

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
WO2003082926A2 (en) * 2002-03-26 2003-10-09 Biosynexus Incorporated Antimicrobial polymer conjugates
CN101717449B (zh) * 2008-10-09 2013-06-19 重庆富进生物医药有限公司 重组TRAIL-Fc融合蛋白及其制备和应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3523701A1 (de) * 1985-07-03 1987-01-08 Bayer Ag Verfahren zur herstellung von proteinen und polypeptiden

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221837B1 (en) 1996-07-26 2001-04-24 Aventis Pharma Deutschland Gmbh Insulin derivatives with increased zinc binding
US6686177B1 (en) 1998-06-06 2004-02-03 Aventis Pharma Deutschland Gmbh Insulin analogs with enhanced zinc binding

Also Published As

Publication number Publication date
DE3873273D1 (de) 1992-09-03
HU204894B (en) 1992-02-28
NO885154L (no) 1989-05-22
DK645588D0 (da) 1988-11-18
IL88413A0 (en) 1989-06-30
IE883462L (en) 1989-05-20
NZ226998A (en) 1991-06-25
AU2569588A (en) 1989-06-29
EP0316748A2 (de) 1989-05-24
IE62228B1 (en) 1995-01-11
ATE78873T1 (de) 1992-08-15
FI885328A7 (fi) 1989-05-21
FI885328L (fi) 1989-05-21
CA1337283C (en) 1995-10-10
CN1033184A (zh) 1989-05-31
PT89019B (pt) 1993-02-26
ZA888650B (en) 1989-07-26
EP0316748A3 (en) 1990-08-29
FI885328A0 (fi) 1988-11-17
JPH01160496A (ja) 1989-06-23
AU618035B2 (en) 1991-12-12
IL88413A (en) 1993-07-08
PT89019A (pt) 1988-12-01
KR890008166A (ko) 1989-07-10
NO173194C (no) 1993-11-10
HUT50514A (en) 1990-02-28
NO173194B (no) 1993-08-02
EP0316748B1 (de) 1992-07-29
ES2051816T3 (es) 1994-07-01
GR3006045T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1993-06-21
NO885154D0 (no) 1988-11-18
DK645588A (da) 1989-05-21

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