AU2569588A - A method for the selective cleavage of fusion proteins - Google Patents

A method for the selective cleavage of fusion proteins

Info

Publication number
AU2569588A
AU2569588A AU25695/88A AU2569588A AU2569588A AU 2569588 A AU2569588 A AU 2569588A AU 25695/88 A AU25695/88 A AU 25695/88A AU 2569588 A AU2569588 A AU 2569588A AU 2569588 A AU2569588 A AU 2569588A
Authority
AU
Australia
Prior art keywords
fusion proteins
selective cleavage
cleavage
proteins
selective
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU25695/88A
Other versions
AU618035B2 (en
Inventor
Michael Dorschug
Gerhard Seipke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of AU2569588A publication Critical patent/AU2569588A/en
Application granted granted Critical
Publication of AU618035B2 publication Critical patent/AU618035B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

Polypeptides or proteins are prepared by enzymatic cleavage of the oligo- or polyglycine sequence of a fusion protein by means of endoprotease.
AU25695/88A 1987-11-20 1988-11-18 A method for the selective cleavage of fusion proteins Expired AU618035B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3739347 1987-11-20
DE19873739347 DE3739347A1 (en) 1987-11-20 1987-11-20 METHOD FOR SELECTIVE CLEAVAGE OF FUSION PROTEINS

Publications (2)

Publication Number Publication Date
AU2569588A true AU2569588A (en) 1989-06-29
AU618035B2 AU618035B2 (en) 1991-12-12

Family

ID=6340882

Family Applications (1)

Application Number Title Priority Date Filing Date
AU25695/88A Expired AU618035B2 (en) 1987-11-20 1988-11-18 A method for the selective cleavage of fusion proteins

Country Status (19)

Country Link
EP (1) EP0316748B1 (en)
JP (1) JPH01160496A (en)
KR (1) KR890008166A (en)
CN (1) CN1033184A (en)
AT (1) ATE78873T1 (en)
AU (1) AU618035B2 (en)
CA (1) CA1337283C (en)
DE (2) DE3739347A1 (en)
DK (1) DK645588A (en)
ES (1) ES2051816T3 (en)
FI (1) FI885328A (en)
GR (1) GR3006045T3 (en)
HU (1) HU204894B (en)
IE (1) IE62228B1 (en)
IL (1) IL88413A (en)
NO (1) NO173194C (en)
NZ (1) NZ226998A (en)
PT (1) PT89019B (en)
ZA (1) ZA888650B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
PT821006E (en) 1996-07-26 2004-09-30 Aventis Pharma Gmbh INSULIN DERIVATIVES WITH ENHANCED ZINC LINK
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
DE19825447A1 (en) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh New insulin analogues with increased zinc formation
CA2479807A1 (en) * 2002-03-26 2003-10-09 Biosynexus Incorporated Antimicrobial polymer conjugates
CN101717449B (en) * 2008-10-09 2013-06-19 重庆富进生物医药有限公司 Recombinant TRAIL-Fc fusion protein as well as preparation and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3523701A1 (en) * 1985-07-03 1987-01-08 Bayer Ag PROCESS FOR PRODUCING PROTEINS AND POLYPEPTIDES

Also Published As

Publication number Publication date
IE883462L (en) 1989-05-20
EP0316748A2 (en) 1989-05-24
FI885328A0 (en) 1988-11-17
DK645588D0 (en) 1988-11-18
CN1033184A (en) 1989-05-31
NO885154L (en) 1989-05-22
ZA888650B (en) 1989-07-26
EP0316748B1 (en) 1992-07-29
IL88413A0 (en) 1989-06-30
KR890008166A (en) 1989-07-10
DK645588A (en) 1989-05-21
IE62228B1 (en) 1995-01-11
DE3873273D1 (en) 1992-09-03
HU204894B (en) 1992-02-28
NO173194B (en) 1993-08-02
JPH01160496A (en) 1989-06-23
IL88413A (en) 1993-07-08
GR3006045T3 (en) 1993-06-21
ATE78873T1 (en) 1992-08-15
AU618035B2 (en) 1991-12-12
PT89019B (en) 1993-02-26
NO173194C (en) 1993-11-10
NZ226998A (en) 1991-06-25
FI885328A (en) 1989-05-21
ES2051816T3 (en) 1994-07-01
HUT50514A (en) 1990-02-28
PT89019A (en) 1988-12-01
NO885154D0 (en) 1988-11-18
EP0316748A3 (en) 1990-08-29
CA1337283C (en) 1995-10-10
DE3739347A1 (en) 1989-06-01

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