DE3518527A1 - Fluorometer auf impulsbasis - Google Patents
Fluorometer auf impulsbasisInfo
- Publication number
- DE3518527A1 DE3518527A1 DE19853518527 DE3518527A DE3518527A1 DE 3518527 A1 DE3518527 A1 DE 3518527A1 DE 19853518527 DE19853518527 DE 19853518527 DE 3518527 A DE3518527 A DE 3518527A DE 3518527 A1 DE3518527 A1 DE 3518527A1
- Authority
- DE
- Germany
- Prior art keywords
- light
- pulse
- led
- pulses
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005259 measurement Methods 0.000 claims abstract description 19
- 230000005284 excitation Effects 0.000 claims abstract description 5
- 230000003287 optical effect Effects 0.000 claims description 16
- 239000000835 fiber Substances 0.000 claims description 8
- 239000013307 optical fiber Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 229920003002 synthetic resin Polymers 0.000 claims description 3
- 239000000057 synthetic resin Substances 0.000 claims description 3
- 230000001276 controlling effect Effects 0.000 claims description 2
- 230000000737 periodic effect Effects 0.000 claims description 2
- 239000003990 capacitor Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000001960 triggered effect Effects 0.000 claims 1
- 229930002875 chlorophyll Natural products 0.000 abstract description 6
- 235000019804 chlorophyll Nutrition 0.000 abstract description 6
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 241000219315 Spinacia Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001795 light effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000000886 photobiology Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
- G01N2201/0621—Supply
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/069—Supply of sources
- G01N2201/0696—Pulsed
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19853518527 DE3518527A1 (de) | 1985-05-23 | 1985-05-23 | Fluorometer auf impulsbasis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19853518527 DE3518527A1 (de) | 1985-05-23 | 1985-05-23 | Fluorometer auf impulsbasis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3518527A1 true DE3518527A1 (de) | 1986-11-27 |
| DE3518527C2 DE3518527C2 (enrdf_load_stackoverflow) | 1987-07-16 |
Family
ID=6271436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19853518527 Granted DE3518527A1 (de) | 1985-05-23 | 1985-05-23 | Fluorometer auf impulsbasis |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3518527A1 (enrdf_load_stackoverflow) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987006698A1 (en) * | 1986-04-30 | 1987-11-05 | Roland Wass | Apparatus for measuring the efficiency of photosynthesis of plants |
| EP0237363A3 (en) * | 1986-03-14 | 1989-10-18 | Norrish, Richard John | Improvements in measurement of fluorescence |
| WO1991010352A1 (en) * | 1990-01-10 | 1991-07-25 | Lännen Tehtaat Oy | Method for the detection of plants |
| NL9101639A (nl) * | 1991-09-27 | 1992-01-02 | Ears Ingenieursbureau Voor Env | Werkwijze en meetinrichting voor het bepalen van het quantum-rendement van de fotosynthese. |
| DE4140414A1 (de) * | 1991-12-07 | 1993-06-09 | Christian 2300 Kiel De Moldaenke | Verfahren zur messung der fluoreszenzrueckmeldung von algen |
| WO2000075642A1 (de) * | 1999-06-06 | 2000-12-14 | Planto Gmbh | Gerät zur messung von laserinduzierter fluoreszenz von pigmenten und/oder umweltschadstoffen |
| WO2002006795A3 (en) * | 2000-07-14 | 2002-04-25 | United Kingdom Government | A method and apparatus for monitoring a condition in chlorophyll containing matter |
| FR2830325A1 (fr) * | 2001-09-28 | 2003-04-04 | Centre Nat Rech Scient | Dispositif de mesure des caracteristiques d'absorption lumineuse d'un echantillon de tissu biologique, procede de mesure associe, et applications dans le domaine de l'analyse vegetale et le domaine medical |
| EP1519169A3 (en) * | 2003-09-23 | 2006-11-08 | X-Rite, Inc. | Color measurement instrument using temperature controlled illuminators |
| US7199376B2 (en) | 2000-07-14 | 2007-04-03 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Method and apparatus for monitoring a condition in chlorophyll containing matter |
| WO2007063457A3 (en) * | 2005-11-29 | 2007-11-01 | Koninkl Philips Electronics Nv | Bio chip device with a sample compartment and a light sensitive element, method for the detection of fluorescent particles within at least one sample compartment of a bio chip device |
| US7564046B1 (en) * | 2004-12-15 | 2009-07-21 | Turner Designs, Inc. | Method and apparatus for measuring active fluorescence |
| DE102008018475A1 (de) * | 2008-04-11 | 2009-10-15 | Carl Zeiss Ag | Vorrichtung und Verfahren zur Lumineszenzmessung |
| CN101865846A (zh) * | 2010-06-11 | 2010-10-20 | 浙江大学 | 植物叶绿素荧光在线检测装置 |
| PT107052A (pt) * | 2013-07-09 | 2015-01-09 | Univ Aveiro | Sistema e método de medição da resposta da fluorescência da clorofila a a variações da intensidade ou qualidade da luz |
| DE102013108189A1 (de) * | 2013-07-31 | 2015-02-05 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Anordnung zur optischen Messung einer Prozessgröße und Messgerät umfassend eine solche |
| DE102018122510A1 (de) * | 2018-09-14 | 2020-03-19 | Endress+Hauser Conducta Gmbh+Co. Kg | Optischer Sensor |
| US11448630B2 (en) | 2019-02-08 | 2022-09-20 | Rensselaer Polytechnic Institute | Plant fluorometer for remote detection of growth dynamics |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4332163A1 (de) * | 1993-09-22 | 1995-03-23 | Kolibri Umweltanalytik Und On | Verfahren und Gerät zur Schadstoffanalyse von Gewässerproben |
| DE19910436A1 (de) * | 1999-03-10 | 2000-10-12 | Ulrich Schreiber | Zweikanal-Chlorophyllfluorometer für Toxizität - Biotests |
| DE10242529A1 (de) * | 2002-09-12 | 2004-03-25 | Forschungszentrum Jülich GmbH | Meßsystem mit photoempfindlicher Elektrode |
| DE10242530A1 (de) * | 2002-09-12 | 2004-03-25 | Forschungszentrum Jülich GmbH | Lichtquelle für ein Meßsystem mit photoempfindlicher Elektrode zum Nachweis eines oder mehrerer Analyten |
| DE102005061674B4 (de) * | 2005-12-21 | 2008-01-10 | Hochschule Mannheim | Faseroptisches Fluoreszenzsensorsystem |
| CN102519880B (zh) * | 2011-12-27 | 2013-05-01 | 中国计量学院 | 厚度补偿式叶绿素仪 |
| CN108920857B (zh) * | 2018-07-10 | 2020-06-02 | 南京大学 | 一种日光诱导叶绿素荧光的模拟方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3811777A (en) * | 1973-02-06 | 1974-05-21 | Johnson Res Foundation Medical | Time-sharing fluorometer and reflectometer |
| US3830222A (en) * | 1972-07-07 | 1974-08-20 | Johnson Res Foundation | Method and apparatus for observing rates of reaction of oxygen in living tissues |
| US4084905A (en) * | 1976-03-11 | 1978-04-18 | Canadian Patents & Development Limited | Apparatus for detecting and measuring fluorescence emission |
| DE3303510A1 (de) * | 1983-01-31 | 1983-07-14 | Peter Dr.-Ing. Gräber | Rechnergesteuertes fluorometer mit auswerteeinheit zur schadstoffdetektion an intakten pflanzen und isolierten chloroplasten |
| DE2914147C2 (de) * | 1978-07-21 | 1984-05-03 | Früngel, Frank, Dr.-Ing., 2000 Hamburg | In-situ Fluorometer für den Unterwassereinsatz |
| GB2131165A (en) * | 1982-09-28 | 1984-06-13 | Hamamatsu Photonics Kk | Device for measuring low intensity light |
-
1985
- 1985-05-23 DE DE19853518527 patent/DE3518527A1/de active Granted
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3830222A (en) * | 1972-07-07 | 1974-08-20 | Johnson Res Foundation | Method and apparatus for observing rates of reaction of oxygen in living tissues |
| US3811777A (en) * | 1973-02-06 | 1974-05-21 | Johnson Res Foundation Medical | Time-sharing fluorometer and reflectometer |
| US4084905A (en) * | 1976-03-11 | 1978-04-18 | Canadian Patents & Development Limited | Apparatus for detecting and measuring fluorescence emission |
| DE2914147C2 (de) * | 1978-07-21 | 1984-05-03 | Früngel, Frank, Dr.-Ing., 2000 Hamburg | In-situ Fluorometer für den Unterwassereinsatz |
| GB2131165A (en) * | 1982-09-28 | 1984-06-13 | Hamamatsu Photonics Kk | Device for measuring low intensity light |
| DE3303510A1 (de) * | 1983-01-31 | 1983-07-14 | Peter Dr.-Ing. Gräber | Rechnergesteuertes fluorometer mit auswerteeinheit zur schadstoffdetektion an intakten pflanzen und isolierten chloroplasten |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0237363A3 (en) * | 1986-03-14 | 1989-10-18 | Norrish, Richard John | Improvements in measurement of fluorescence |
| WO1987006698A1 (en) * | 1986-04-30 | 1987-11-05 | Roland Wass | Apparatus for measuring the efficiency of photosynthesis of plants |
| WO1991010352A1 (en) * | 1990-01-10 | 1991-07-25 | Lännen Tehtaat Oy | Method for the detection of plants |
| NL9101639A (nl) * | 1991-09-27 | 1992-01-02 | Ears Ingenieursbureau Voor Env | Werkwijze en meetinrichting voor het bepalen van het quantum-rendement van de fotosynthese. |
| DE4140414A1 (de) * | 1991-12-07 | 1993-06-09 | Christian 2300 Kiel De Moldaenke | Verfahren zur messung der fluoreszenzrueckmeldung von algen |
| WO1993012415A1 (de) * | 1991-12-07 | 1993-06-24 | Christian Moldaenke | Verfahren und vorrichtung zur messung der fluoreszenzrückmeldung von algen |
| WO2000075642A1 (de) * | 1999-06-06 | 2000-12-14 | Planto Gmbh | Gerät zur messung von laserinduzierter fluoreszenz von pigmenten und/oder umweltschadstoffen |
| WO2002006795A3 (en) * | 2000-07-14 | 2002-04-25 | United Kingdom Government | A method and apparatus for monitoring a condition in chlorophyll containing matter |
| US7199376B2 (en) | 2000-07-14 | 2007-04-03 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Method and apparatus for monitoring a condition in chlorophyll containing matter |
| FR2830325A1 (fr) * | 2001-09-28 | 2003-04-04 | Centre Nat Rech Scient | Dispositif de mesure des caracteristiques d'absorption lumineuse d'un echantillon de tissu biologique, procede de mesure associe, et applications dans le domaine de l'analyse vegetale et le domaine medical |
| WO2003029791A1 (fr) * | 2001-09-28 | 2003-04-10 | Centre National De La Recherche Scientifique (C.N.R.S.) | Dispositif de mesure des caracteristiques d'absorption lumineuse d'un echantillon de tissu biologique |
| US7368694B2 (en) | 2001-09-28 | 2008-05-06 | Centre National De La Recherche Scientifique (C.N.R.S) | Device for measuring light absorption characteristics of a biological tissue sample |
| US7262853B2 (en) | 2003-09-23 | 2007-08-28 | X-Rite, Inc. | Color measurement instrument |
| EP1519169A3 (en) * | 2003-09-23 | 2006-11-08 | X-Rite, Inc. | Color measurement instrument using temperature controlled illuminators |
| US7564046B1 (en) * | 2004-12-15 | 2009-07-21 | Turner Designs, Inc. | Method and apparatus for measuring active fluorescence |
| WO2007063457A3 (en) * | 2005-11-29 | 2007-11-01 | Koninkl Philips Electronics Nv | Bio chip device with a sample compartment and a light sensitive element, method for the detection of fluorescent particles within at least one sample compartment of a bio chip device |
| DE102008018475A1 (de) * | 2008-04-11 | 2009-10-15 | Carl Zeiss Ag | Vorrichtung und Verfahren zur Lumineszenzmessung |
| US9007579B2 (en) | 2008-04-11 | 2015-04-14 | Carl Zeiss Microscopy Gmbh | Device and method for measuring luminescence |
| CN101865846A (zh) * | 2010-06-11 | 2010-10-20 | 浙江大学 | 植物叶绿素荧光在线检测装置 |
| PT107052B (pt) * | 2013-07-09 | 2015-10-05 | Univ Aveiro | Sistema e método de medição da resposta da fluorescência da clorofila a a variações da intensidade ou qualidade da luz |
| PT107052A (pt) * | 2013-07-09 | 2015-01-09 | Univ Aveiro | Sistema e método de medição da resposta da fluorescência da clorofila a a variações da intensidade ou qualidade da luz |
| DE102013108189A1 (de) * | 2013-07-31 | 2015-02-05 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Anordnung zur optischen Messung einer Prozessgröße und Messgerät umfassend eine solche |
| US9488581B2 (en) | 2013-07-31 | 2016-11-08 | Endress+Hauser Conducta Gmbh+Co. Kg | Arrangement for optical measurement of a process variable and measuring device comprising such an arrangement |
| DE102018122510A1 (de) * | 2018-09-14 | 2020-03-19 | Endress+Hauser Conducta Gmbh+Co. Kg | Optischer Sensor |
| US11448630B2 (en) | 2019-02-08 | 2022-09-20 | Rensselaer Polytechnic Institute | Plant fluorometer for remote detection of growth dynamics |
| US11965869B2 (en) | 2019-02-08 | 2024-04-23 | Rensselaer Polytechnic Institute | Plant fluorometer for remote detection of growth dynamics |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3518527C2 (enrdf_load_stackoverflow) | 1987-07-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |