DE3036184A1 - Reagenz und verfahren zur bestimmung von human-muskeltyp-aldolase - Google Patents
Reagenz und verfahren zur bestimmung von human-muskeltyp-aldolaseInfo
- Publication number
- DE3036184A1 DE3036184A1 DE19803036184 DE3036184A DE3036184A1 DE 3036184 A1 DE3036184 A1 DE 3036184A1 DE 19803036184 DE19803036184 DE 19803036184 DE 3036184 A DE3036184 A DE 3036184A DE 3036184 A1 DE3036184 A1 DE 3036184A1
- Authority
- DE
- Germany
- Prior art keywords
- muscle
- type aldolase
- antibody
- aldolase
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 101000755879 Homo sapiens Fructose-bisphosphate aldolase A Proteins 0.000 title claims description 47
- 238000000034 method Methods 0.000 title claims description 42
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 18
- 101710123627 Fructose-bisphosphate aldolase A Proteins 0.000 claims description 70
- 102100022277 Fructose-bisphosphate aldolase A Human genes 0.000 claims description 70
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 50
- 102000004190 Enzymes Human genes 0.000 claims description 40
- 108090000790 Enzymes Proteins 0.000 claims description 40
- 239000007853 buffer solution Substances 0.000 claims description 21
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 claims description 13
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 claims description 13
- 210000002966 serum Anatomy 0.000 claims description 13
- 239000000758 substrate Substances 0.000 claims description 12
- 238000010521 absorption reaction Methods 0.000 claims description 11
- 239000007790 solid phase Substances 0.000 claims description 10
- 241000283690 Bos taurus Species 0.000 claims description 7
- 238000001042 affinity chromatography Methods 0.000 claims description 7
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 5
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 238000000354 decomposition reaction Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 description 34
- 239000000243 solution Substances 0.000 description 26
- 238000002474 experimental method Methods 0.000 description 23
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 15
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 15
- 239000000427 antigen Substances 0.000 description 12
- 210000003205 muscle Anatomy 0.000 description 12
- 102000036639 antigens Human genes 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000003018 immunoassay Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 8
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000004793 Polystyrene Substances 0.000 description 5
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 5
- 229940025237 fructose 1,6-diphosphate Drugs 0.000 description 5
- 229920002223 polystyrene Polymers 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 229920002684 Sepharose Polymers 0.000 description 4
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 108010044467 Isoenzymes Proteins 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- 150000001340 alkali metals Chemical class 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- WRRSFOZOETZUPG-FFHNEAJVSA-N (4r,4ar,7s,7ar,12bs)-9-methoxy-3-methyl-2,4,4a,7,7a,13-hexahydro-1h-4,12-methanobenzofuro[3,2-e]isoquinoline-7-ol;hydrate Chemical compound O.C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OC WRRSFOZOETZUPG-FFHNEAJVSA-N 0.000 description 1
- 241000252073 Anguilliformes Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 241000921769 Arabella Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- ZOKIJEBQDZFGMW-PSXMRANNSA-N [(2R)-2-[12-(4-azido-2-nitroanilino)dodecanoyloxy]-3-tetradecanoyloxypropyl] 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCNc1ccc(cc1[N+]([O-])=O)N=[N+]=[N-] ZOKIJEBQDZFGMW-PSXMRANNSA-N 0.000 description 1
- WMDDNKROYKCDJC-UHFFFAOYSA-N [4-[3-oxo-1-(4-phosphonooxyphenyl)-2-benzofuran-1-yl]phenyl] dihydrogen phosphate Chemical compound C1=CC(OP(O)(=O)O)=CC=C1C1(C=2C=CC(OP(O)(O)=O)=CC=2)C2=CC=CC=C2C(=O)O1 WMDDNKROYKCDJC-UHFFFAOYSA-N 0.000 description 1
- 150000008065 acid anhydrides Chemical class 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 238000005882 aldol condensation reaction Methods 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- DHCLVCXQIBBOPH-UHFFFAOYSA-N beta-glycerol phosphate Natural products OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 description 1
- GHRQXJHBXKYCLZ-UHFFFAOYSA-L beta-glycerolphosphate Chemical compound [Na+].[Na+].CC(CO)OOP([O-])([O-])=O GHRQXJHBXKYCLZ-UHFFFAOYSA-L 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- ZKLLSNQJRLJIGT-UYFOZJQFSA-N keto-D-fructose 1-phosphate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)COP(O)(O)=O ZKLLSNQJRLJIGT-UYFOZJQFSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- CQTZJAKSNDFPOB-UHFFFAOYSA-L naphthalen-2-yl phosphate Chemical compound C1=CC=CC2=CC(OP([O-])(=O)[O-])=CC=C21 CQTZJAKSNDFPOB-UHFFFAOYSA-L 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- CMPQUABWPXYYSH-UHFFFAOYSA-N phenyl phosphate Chemical compound OP(O)(=O)OC1=CC=CC=C1 CMPQUABWPXYYSH-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12204779A JPS5648894A (en) | 1979-09-25 | 1979-09-25 | Reagent for measuring human muscular aldolase and its determination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3036184A1 true DE3036184A1 (de) | 1981-04-16 |
Family
ID=14826285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19803036184 Withdrawn DE3036184A1 (de) | 1979-09-25 | 1980-09-25 | Reagenz und verfahren zur bestimmung von human-muskeltyp-aldolase |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPS5648894A (enExample) |
| BE (1) | BE885376A (enExample) |
| CA (1) | CA1158549A (enExample) |
| CH (1) | CH648596A5 (enExample) |
| DE (1) | DE3036184A1 (enExample) |
| FR (1) | FR2466020A1 (enExample) |
| GB (1) | GB2062226B (enExample) |
| IT (1) | IT1141082B (enExample) |
| NL (1) | NL8005330A (enExample) |
| SE (1) | SE447422B (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3145936A1 (de) * | 1981-11-20 | 1983-06-01 | Behringwerke Ag, 3550 Marburg | "verfahren zur enzymimmunologischen bestimmung von lipase" |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56125667A (en) * | 1980-03-07 | 1981-10-02 | Eisai Co Ltd | Reagent for measurement and measuring method for human muscle type aldolase |
| JPS57208459A (en) * | 1981-06-19 | 1982-12-21 | Eisai Co Ltd | Measuring method using enzyme-labelled antibody and reagent |
| JP6754116B2 (ja) * | 2016-04-26 | 2020-09-09 | 学校法人近畿大学 | 大腸癌マーカー |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2128670B2 (de) * | 1971-06-09 | 1977-06-30 | Merck Patent Gmbh, 6100 Darmstadt | Immunologische isoenzym-bestimmungsmethode |
| JPS54147097A (en) * | 1978-05-11 | 1979-11-16 | Eisai Co Ltd | Reagent for detecting muscleetype aldolase |
-
1979
- 1979-09-25 JP JP12204779A patent/JPS5648894A/ja active Pending
-
1980
- 1980-09-23 CH CH7209/80A patent/CH648596A5/de not_active IP Right Cessation
- 1980-09-24 GB GB8030730A patent/GB2062226B/en not_active Expired
- 1980-09-24 CA CA000360973A patent/CA1158549A/en not_active Expired
- 1980-09-24 BE BE0/202218A patent/BE885376A/fr not_active IP Right Cessation
- 1980-09-24 IT IT24877/80A patent/IT1141082B/it active
- 1980-09-24 SE SE8006667A patent/SE447422B/sv not_active IP Right Cessation
- 1980-09-24 NL NL8005330A patent/NL8005330A/nl not_active Application Discontinuation
- 1980-09-24 FR FR8020472A patent/FR2466020A1/fr active Granted
- 1980-09-25 DE DE19803036184 patent/DE3036184A1/de not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3145936A1 (de) * | 1981-11-20 | 1983-06-01 | Behringwerke Ag, 3550 Marburg | "verfahren zur enzymimmunologischen bestimmung von lipase" |
Also Published As
| Publication number | Publication date |
|---|---|
| IT8024877A0 (it) | 1980-09-24 |
| JPS5648894A (en) | 1981-05-02 |
| CH648596A5 (de) | 1985-03-29 |
| SE8006667L (sv) | 1981-03-26 |
| FR2466020B1 (enExample) | 1983-07-08 |
| IT1141082B (it) | 1986-10-01 |
| SE447422B (sv) | 1986-11-10 |
| CA1158549A (en) | 1983-12-13 |
| BE885376A (fr) | 1981-03-24 |
| GB2062226B (en) | 1983-11-30 |
| FR2466020A1 (fr) | 1981-03-27 |
| NL8005330A (nl) | 1981-03-27 |
| GB2062226A (en) | 1981-05-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8141 | Disposal/no request for examination |