JPS5648894A - Reagent for measuring human muscular aldolase and its determination - Google Patents

Reagent for measuring human muscular aldolase and its determination

Info

Publication number
JPS5648894A
JPS5648894A JP12204779A JP12204779A JPS5648894A JP S5648894 A JPS5648894 A JP S5648894A JP 12204779 A JP12204779 A JP 12204779A JP 12204779 A JP12204779 A JP 12204779A JP S5648894 A JPS5648894 A JP S5648894A
Authority
JP
Japan
Prior art keywords
solid phase
antibody
specimen
antigen
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP12204779A
Other languages
Japanese (ja)
Inventor
Toyohiro Kitamura
Tomiaki Morimoto
Toru Naraki
Takashi Sawada
Minoru Toda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eisai Co Ltd
Original Assignee
Eisai Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai Co Ltd filed Critical Eisai Co Ltd
Priority to JP12204779A priority Critical patent/JPS5648894A/en
Priority to CH7209/80A priority patent/CH648596A5/en
Priority to NL8005330A priority patent/NL8005330A/en
Priority to GB8030730A priority patent/GB2062226B/en
Priority to SE8006667A priority patent/SE447422B/en
Priority to CA000360973A priority patent/CA1158549A/en
Priority to BE0/202218A priority patent/BE885376A/en
Priority to IT24877/80A priority patent/IT1141082B/en
Priority to FR8020472A priority patent/FR2466020A1/en
Priority to DE19803036184 priority patent/DE3036184A1/en
Publication of JPS5648894A publication Critical patent/JPS5648894A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE: The titled reagent eliminating or correcting defects, e.g., inacurate quantitative determination, complex handling, etc. resulting from conventional electrophoresis, method by carrying out at enzyme activity ratio of FDP/FIP, etc., comprising muscular aldolase antibody of enzyme marker and its determination.
CONSTITUTION: An antibody combined with a carrier to be in solid phase is brought into contact with a specimen, e.g., serum, etc. so that the antigen in the specimen (human muscular aldolase) is reacted with the antibody in solid phase, the reaction solution is removed and the solid phase is washed with a buffer solution, etc. [process (a)]. The solid phase obtained in the process (a) is brought into contact with an antibody of enzyme marker so that the antibody of enzyme marker is the first bonded to the antigen which is linked to the antibody in solid phase, the reaction solution is removed and the solid phase is washed [process (b)]. The solid phase obtained in the process (b) is brought into contact and reacted enzymically with a base of enzyme [process (c)]. The absorbency of the decomposed product of the base in the reaction solution of the process (c) is measured by a spectrophotometer using wavelength suitable for the qualitative determination [process (d)]. In the above-mentioned measuring system, the absorbencies of standard specimens whose amounts have been known are previously measured so that a calibration curve between amounts of the antigen and the absorbencies is determined. An amount of the antigen in the specimen is found from the absorbency of the specimen using the calibration curve.
COPYRIGHT: (C)1981,JPO&Japio
JP12204779A 1979-09-25 1979-09-25 Reagent for measuring human muscular aldolase and its determination Pending JPS5648894A (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
JP12204779A JPS5648894A (en) 1979-09-25 1979-09-25 Reagent for measuring human muscular aldolase and its determination
CH7209/80A CH648596A5 (en) 1979-09-25 1980-09-23 REAGENT AND METHOD FOR DETERMINING HUMAN MUSCLE TYPE ALDOLASE.
NL8005330A NL8005330A (en) 1979-09-25 1980-09-24 REAGENT AND METHOD FOR DETERMINING ALDOLASE OF THE HUMAN MUSCLE TYPE.
GB8030730A GB2062226B (en) 1979-09-25 1980-09-24 Reagent and method for the determination of human-muscle adolase
SE8006667A SE447422B (en) 1979-09-25 1980-09-24 REAGENTS AND SETS FOR DETERMINING ALDOLAS OF THE TYPE THAT ARE IN THE HUMAN MUSCLES
CA000360973A CA1158549A (en) 1979-09-25 1980-09-24 Enzyme-labelled muscle type aldolase antibody
BE0/202218A BE885376A (en) 1979-09-25 1980-09-24 REAGENT AND METHOD FOR DETERMINING THE ALDOLASE OF THE TYPE CONTAINED IN THE MUSCLES OF MAN
IT24877/80A IT1141082B (en) 1979-09-25 1980-09-24 REAGENT AND METHOD TO DETERMINE HUMAN MUSCULAR ALDOLASE
FR8020472A FR2466020A1 (en) 1979-09-25 1980-09-24 REAGENT AND METHOD FOR THE DETERMINATION OF ALDOLASE OF THE TYPE CONTENT IN MUSCLES OF MAN
DE19803036184 DE3036184A1 (en) 1979-09-25 1980-09-25 REAGENT AND METHOD FOR DETERMINING HUMAN MUSCLE TYPE ALDOLASE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12204779A JPS5648894A (en) 1979-09-25 1979-09-25 Reagent for measuring human muscular aldolase and its determination

Publications (1)

Publication Number Publication Date
JPS5648894A true JPS5648894A (en) 1981-05-02

Family

ID=14826285

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12204779A Pending JPS5648894A (en) 1979-09-25 1979-09-25 Reagent for measuring human muscular aldolase and its determination

Country Status (10)

Country Link
JP (1) JPS5648894A (en)
BE (1) BE885376A (en)
CA (1) CA1158549A (en)
CH (1) CH648596A5 (en)
DE (1) DE3036184A1 (en)
FR (1) FR2466020A1 (en)
GB (1) GB2062226B (en)
IT (1) IT1141082B (en)
NL (1) NL8005330A (en)
SE (1) SE447422B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017198509A (en) * 2016-04-26 2017-11-02 学校法人近畿大学 Colorectal cancer marker

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56125667A (en) * 1980-03-07 1981-10-02 Eisai Co Ltd Reagent for measurement and measuring method for human muscle type aldolase
JPS57208459A (en) * 1981-06-19 1982-12-21 Eisai Co Ltd Measuring method using enzyme-labelled antibody and reagent
DE3145936A1 (en) * 1981-11-20 1983-06-01 Behringwerke Ag, 3550 Marburg Method for the enzyme immunological determination of lipase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2128670B2 (en) * 1971-06-09 1977-06-30 Merck Patent Gmbh, 6100 Darmstadt IMMUNOLOGICAL ISOENZYME DETERMINATION METHOD
JPS54147097A (en) * 1978-05-11 1979-11-16 Eisai Co Ltd Reagent for detecting muscleetype aldolase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017198509A (en) * 2016-04-26 2017-11-02 学校法人近畿大学 Colorectal cancer marker

Also Published As

Publication number Publication date
FR2466020B1 (en) 1983-07-08
BE885376A (en) 1981-03-24
IT1141082B (en) 1986-10-01
GB2062226A (en) 1981-05-20
CH648596A5 (en) 1985-03-29
DE3036184A1 (en) 1981-04-16
CA1158549A (en) 1983-12-13
IT8024877A0 (en) 1980-09-24
GB2062226B (en) 1983-11-30
SE8006667L (en) 1981-03-26
NL8005330A (en) 1981-03-27
SE447422B (en) 1986-11-10
FR2466020A1 (en) 1981-03-27

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