JPS5648894A - Reagent for measuring human muscular aldolase and its determination - Google Patents
Reagent for measuring human muscular aldolase and its determinationInfo
- Publication number
- JPS5648894A JPS5648894A JP12204779A JP12204779A JPS5648894A JP S5648894 A JPS5648894 A JP S5648894A JP 12204779 A JP12204779 A JP 12204779A JP 12204779 A JP12204779 A JP 12204779A JP S5648894 A JPS5648894 A JP S5648894A
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- antibody
- specimen
- antigen
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: The titled reagent eliminating or correcting defects, e.g., inacurate quantitative determination, complex handling, etc. resulting from conventional electrophoresis, method by carrying out at enzyme activity ratio of FDP/FIP, etc., comprising muscular aldolase antibody of enzyme marker and its determination.
CONSTITUTION: An antibody combined with a carrier to be in solid phase is brought into contact with a specimen, e.g., serum, etc. so that the antigen in the specimen (human muscular aldolase) is reacted with the antibody in solid phase, the reaction solution is removed and the solid phase is washed with a buffer solution, etc. [process (a)]. The solid phase obtained in the process (a) is brought into contact with an antibody of enzyme marker so that the antibody of enzyme marker is the first bonded to the antigen which is linked to the antibody in solid phase, the reaction solution is removed and the solid phase is washed [process (b)]. The solid phase obtained in the process (b) is brought into contact and reacted enzymically with a base of enzyme [process (c)]. The absorbency of the decomposed product of the base in the reaction solution of the process (c) is measured by a spectrophotometer using wavelength suitable for the qualitative determination [process (d)]. In the above-mentioned measuring system, the absorbencies of standard specimens whose amounts have been known are previously measured so that a calibration curve between amounts of the antigen and the absorbencies is determined. An amount of the antigen in the specimen is found from the absorbency of the specimen using the calibration curve.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12204779A JPS5648894A (en) | 1979-09-25 | 1979-09-25 | Reagent for measuring human muscular aldolase and its determination |
CH7209/80A CH648596A5 (en) | 1979-09-25 | 1980-09-23 | REAGENT AND METHOD FOR DETERMINING HUMAN MUSCLE TYPE ALDOLASE. |
BE0/202218A BE885376A (en) | 1979-09-25 | 1980-09-24 | REAGENT AND METHOD FOR DETERMINING THE ALDOLASE OF THE TYPE CONTAINED IN THE MUSCLES OF MAN |
GB8030730A GB2062226B (en) | 1979-09-25 | 1980-09-24 | Reagent and method for the determination of human-muscle adolase |
SE8006667A SE447422B (en) | 1979-09-25 | 1980-09-24 | REAGENTS AND SETS FOR DETERMINING ALDOLAS OF THE TYPE THAT ARE IN THE HUMAN MUSCLES |
IT24877/80A IT1141082B (en) | 1979-09-25 | 1980-09-24 | REAGENT AND METHOD TO DETERMINE HUMAN MUSCULAR ALDOLASE |
CA000360973A CA1158549A (en) | 1979-09-25 | 1980-09-24 | Enzyme-labelled muscle type aldolase antibody |
FR8020472A FR2466020A1 (en) | 1979-09-25 | 1980-09-24 | REAGENT AND METHOD FOR THE DETERMINATION OF ALDOLASE OF THE TYPE CONTENT IN MUSCLES OF MAN |
NL8005330A NL8005330A (en) | 1979-09-25 | 1980-09-24 | REAGENT AND METHOD FOR DETERMINING ALDOLASE OF THE HUMAN MUSCLE TYPE. |
DE19803036184 DE3036184A1 (en) | 1979-09-25 | 1980-09-25 | REAGENT AND METHOD FOR DETERMINING HUMAN MUSCLE TYPE ALDOLASE |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12204779A JPS5648894A (en) | 1979-09-25 | 1979-09-25 | Reagent for measuring human muscular aldolase and its determination |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5648894A true JPS5648894A (en) | 1981-05-02 |
Family
ID=14826285
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12204779A Pending JPS5648894A (en) | 1979-09-25 | 1979-09-25 | Reagent for measuring human muscular aldolase and its determination |
Country Status (10)
Country | Link |
---|---|
JP (1) | JPS5648894A (en) |
BE (1) | BE885376A (en) |
CA (1) | CA1158549A (en) |
CH (1) | CH648596A5 (en) |
DE (1) | DE3036184A1 (en) |
FR (1) | FR2466020A1 (en) |
GB (1) | GB2062226B (en) |
IT (1) | IT1141082B (en) |
NL (1) | NL8005330A (en) |
SE (1) | SE447422B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017198509A (en) * | 2016-04-26 | 2017-11-02 | 学校法人近畿大学 | Colorectal cancer marker |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56125667A (en) * | 1980-03-07 | 1981-10-02 | Eisai Co Ltd | Reagent for measurement and measuring method for human muscle type aldolase |
JPS57208459A (en) * | 1981-06-19 | 1982-12-21 | Eisai Co Ltd | Measuring method using enzyme-labelled antibody and reagent |
DE3145936A1 (en) * | 1981-11-20 | 1983-06-01 | Behringwerke Ag, 3550 Marburg | Method for the enzyme immunological determination of lipase |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2128670B2 (en) * | 1971-06-09 | 1977-06-30 | Merck Patent Gmbh, 6100 Darmstadt | IMMUNOLOGICAL ISOENZYME DETERMINATION METHOD |
JPS54147097A (en) * | 1978-05-11 | 1979-11-16 | Eisai Co Ltd | Reagent for detecting muscleetype aldolase |
-
1979
- 1979-09-25 JP JP12204779A patent/JPS5648894A/en active Pending
-
1980
- 1980-09-23 CH CH7209/80A patent/CH648596A5/en not_active IP Right Cessation
- 1980-09-24 IT IT24877/80A patent/IT1141082B/en active
- 1980-09-24 GB GB8030730A patent/GB2062226B/en not_active Expired
- 1980-09-24 CA CA000360973A patent/CA1158549A/en not_active Expired
- 1980-09-24 NL NL8005330A patent/NL8005330A/en not_active Application Discontinuation
- 1980-09-24 BE BE0/202218A patent/BE885376A/en not_active IP Right Cessation
- 1980-09-24 SE SE8006667A patent/SE447422B/en not_active IP Right Cessation
- 1980-09-24 FR FR8020472A patent/FR2466020A1/en active Granted
- 1980-09-25 DE DE19803036184 patent/DE3036184A1/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017198509A (en) * | 2016-04-26 | 2017-11-02 | 学校法人近畿大学 | Colorectal cancer marker |
Also Published As
Publication number | Publication date |
---|---|
BE885376A (en) | 1981-03-24 |
CA1158549A (en) | 1983-12-13 |
NL8005330A (en) | 1981-03-27 |
DE3036184A1 (en) | 1981-04-16 |
SE8006667L (en) | 1981-03-26 |
FR2466020B1 (en) | 1983-07-08 |
GB2062226A (en) | 1981-05-20 |
IT1141082B (en) | 1986-10-01 |
IT8024877A0 (en) | 1980-09-24 |
FR2466020A1 (en) | 1981-03-27 |
SE447422B (en) | 1986-11-10 |
GB2062226B (en) | 1983-11-30 |
CH648596A5 (en) | 1985-03-29 |
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Bowers Jr et al. | International Federation of Clinical Chemistry. Committee on Standards. Expert Panel on Enzymes. Approved recommendation (1978) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 1. General considerations concerning the determination of the catalytic concentration of an enzyme in the blood serum or plasma of man |