EP0238857A3 - An analytical reagent containing monoclonal antibodies to actinobacillus actinomycetemcomitans - Google Patents

An analytical reagent containing monoclonal antibodies to actinobacillus actinomycetemcomitans Download PDF

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Publication number
EP0238857A3
EP0238857A3 EP87102483A EP87102483A EP0238857A3 EP 0238857 A3 EP0238857 A3 EP 0238857A3 EP 87102483 A EP87102483 A EP 87102483A EP 87102483 A EP87102483 A EP 87102483A EP 0238857 A3 EP0238857 A3 EP 0238857A3
Authority
EP
European Patent Office
Prior art keywords
actinobacillus actinomycetemcomitans
present
actinobacillus
carrier
monoclonal antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP87102483A
Other languages
German (de)
French (fr)
Other versions
EP0238857A2 (en
Inventor
Robert Joseph Genco
Joseph James Zambon
Lars Anders Christersson
Mirdza Erika Neiders
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Foundation of State University of New York
Original Assignee
Research Foundation of State University of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Foundation of State University of New York filed Critical Research Foundation of State University of New York
Publication of EP0238857A2 publication Critical patent/EP0238857A2/en
Publication of EP0238857A3 publication Critical patent/EP0238857A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56955—Bacteria involved in periodontal diseases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/948—Microorganisms using viruses or cell lines
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Actinobacillus actinomycetemcomitans has frequently been implicated in juvenile periodontitis. The present mono­ clonal antibodies are specific to Actinobacillus actinomyce­ temcomitans. The present monoclonal antibodies are typically employed as reagents which include an inert carrier, preferably a liquid, such as buffered saiine solution and a preservative. The carrier compositions are suitably selected to provide for the proper dispersal of bacteria, and to preserve the integrity of antigens and supplemental structures. The selection of the proper carrier is especially important in the detection in mix­ tures which include bacteria which produce large amounts of autolytic enzymes such as B. gingivalis. The monoclonal anti­ bodies of the present invention are useful in clinical testing and differentiating antigens in the gingival or subgingival sera. The method of testing and differentiating involves the steps of con­ tacting a sample of the bacterial flora from a lesion or other site with a measured amount of monoclonal antibody specific to a single antigen site on Actinobacillus actinomycetemcomitans and subsequently measuring the number of antibody-antigen complexes formed.
EP87102483A 1986-02-26 1987-02-21 An analytical reagent containing monoclonal antibodies to actinobacillus actinomycetemcomitans Ceased EP0238857A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US833856 1986-02-26
US06/833,856 US4741999A (en) 1986-02-26 1986-02-26 Monoclonal antibodies useful in the identification of microorganisms causing periodontal disease

Publications (2)

Publication Number Publication Date
EP0238857A2 EP0238857A2 (en) 1987-09-30
EP0238857A3 true EP0238857A3 (en) 1987-10-07

Family

ID=25265458

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87102483A Ceased EP0238857A3 (en) 1986-02-26 1987-02-21 An analytical reagent containing monoclonal antibodies to actinobacillus actinomycetemcomitans

Country Status (5)

Country Link
US (1) US4741999A (en)
EP (1) EP0238857A3 (en)
JP (1) JPS62211558A (en)
AU (1) AU602670B2 (en)
CA (1) CA1285222C (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352446A (en) * 1986-03-25 1994-10-04 Council Of Governors Of The United Medical And Dental Schools Of Guy's And St. Thomas's Hospitals Method of treating dental caries with monoclonal antibodies against the antigen I and antigen I/II of streptococcus mutans
ZA878610B (en) * 1986-11-20 1989-06-28 Minnesota Mining & Mfg Method for the evaluation of oral microbes
US5055405A (en) * 1987-11-19 1991-10-08 Warner-Lambert Company Hybridoma cell line and monoclonal antibodies to Treponema species T. denticola JD-1 and Treponema 10A
US5158871A (en) * 1988-02-12 1992-10-27 University Of Connecticut Method of using magnetic particles for isolating, collecting and assaying diagnostic ligates
US5089384A (en) * 1988-11-04 1992-02-18 Amoco Corporation Method and apparatus for selective cell destruction using amplified immunofluorescence
CA2032114A1 (en) * 1990-01-22 1991-07-23 Brian A. Snyder Antibody composition for actinobacillus actinomycetemcomitans and its use in diagnostics
US5334503A (en) * 1991-10-08 1994-08-02 Eastman Kodak Company Test kit and method for the detection of microorganisms associated with periodontal diseases using surfactant mixture as extraction composition
US5726016A (en) * 1995-01-18 1998-03-10 The Trustees Of The University Of Pennsylvania Compositions and methods for diagnosis of diseases associated with actinobacillus actinomycetemcomitans infection

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4067959A (en) * 1976-05-10 1978-01-10 International Diagnostic Technology, Inc. Indirect solid surface test for antigens or antibodies
JPS604422B2 (en) * 1976-10-07 1985-02-04 持田製薬株式会社 Antigen quantification method
JPS54119992A (en) * 1978-03-10 1979-09-18 Lion Dentifrice Co Ltd Reagent for measuring quantity of bacteria relating to disease around tooth
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4421860A (en) * 1980-10-07 1983-12-20 The Regents Of The University Of California Homogeneous fluoroimmunoassay involving autocorrelation processing of optically sensed signals
US4487839A (en) * 1983-01-05 1984-12-11 Ortho Diagnostic Systems Inc. Immunoassay methods employing patterns for the detection of soluble and cell surface antigens
JPH0614052B2 (en) * 1985-01-14 1994-02-23 サンスタ−株式会社 Antigen preparation for periodontal disease diagnosis

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOLOGICAL ABSTRACTS, vol. 82, 1986, abstract no. 102447, Philadelphia, US; M.C. HEALEY et al.: "Use of monoclonal antibodies to identity outer membrane antigens of actinobacillus species", & AM.J.VET. RESEARCH (US), 1986, 47(7), 1446-1451 *
BIOLOGICAL ABSTRACTS, vol. 83, 1987, abstract no. 4790, Philadelphia, US; W.P. McARTHUR et al.: "Detection and serotyping of actinobacillus-actinomycetemcomitans isolates on nitrocellulose paper blots with monoclonal antibodies", & JOURNAL OF CLINICAL PERIODONTOLOGY 1986, 13(7), 684-691 *
BIOLOGICAL ABSTRACTS/RRM, vol. 29, 1985, abstract no. 7873, Philadelphia, US; E. DENARDIN et al.: "Serologic characterization of actinobacillus-actinomycetemcomitans using monoclonal antibodies", & FEDERATION PROCEEDINGS 1985, 44(3), 527 *
CHEMICAL ABSTRACTS, vol. 102, no. 7, 18th February 1985, page 455, columns 1, 2, abstract no. 60488z, Columbus, Ohio, US; J.M. DIRIENZO et al.: "Monoclonal antibodies to leukotoxin of Actinobacillus actinomycetemcomitans", & INFECT. IMMUN. 1985, 47(1), 31-36 *
CHEMICAL ABSTRACTS, vol. 106, no. 1, 5th January 1987, page 252, column 1, abstract no. 2578a, Columbus, Ohio, US; K. NAKAJIMA et al.: "Reagents for diagnosis of periodontal diseases", & JP - A - 61 162 753 (SUNSTAR, INC.) 23-07-1986 *

Also Published As

Publication number Publication date
US4741999A (en) 1988-05-03
CA1285222C (en) 1991-06-25
EP0238857A2 (en) 1987-09-30
AU602670B2 (en) 1990-10-25
JPS62211558A (en) 1987-09-17
AU6873587A (en) 1987-08-27

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Inventor name: ZAMBON, JOSEPH JAMES

Inventor name: CHRISTERSSON, LARS ANDERS

Inventor name: GENCO, ROBERT JOSEPH

Inventor name: NEIDERS, MIRDZA ERIKA