JPS6459157A - Immunological analysis and reagent for immunological analysis using the same - Google Patents

Immunological analysis and reagent for immunological analysis using the same

Info

Publication number
JPS6459157A
JPS6459157A JP21679687A JP21679687A JPS6459157A JP S6459157 A JPS6459157 A JP S6459157A JP 21679687 A JP21679687 A JP 21679687A JP 21679687 A JP21679687 A JP 21679687A JP S6459157 A JPS6459157 A JP S6459157A
Authority
JP
Japan
Prior art keywords
liposome
substance
labeled substance
phospholipid
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP21679687A
Other languages
Japanese (ja)
Other versions
JP2553360B2 (en
Inventor
Mamoru Umeda
Junko Akiyama
Noboru Iwase
Kazuaki Yoshikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nissui Pharmacetuical Co Ltd
Original Assignee
Nissui Pharmacetuical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nissui Pharmacetuical Co Ltd filed Critical Nissui Pharmacetuical Co Ltd
Priority to JP62216796A priority Critical patent/JP2553360B2/en
Publication of JPS6459157A publication Critical patent/JPS6459157A/en
Application granted granted Critical
Publication of JP2553360B2 publication Critical patent/JP2553360B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Dispersion Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)

Abstract

PURPOSE:To achieve a higher measuring sensitivity, by getting an amide type phospholipid contained in phospholipid as component of liposome to improve stability of holding a labeled substance in the liposome. CONSTITUTION:A labeled substance is sealed into a liposome having phospholipid containing an amide type phospholipid and cholesterol as requisite component and an antigen or an antibody reacting specifically with a substance to be inspected is immobilized outside the liposome through a crosslinking agent to obtain a liposome reagent. When the liposome reagent thus obtained, a sample containing the substance being inspected and a complement are mixed in a proper buffer, the complement is activated by an antigen-antibody reaction and the labeled substance is released from the inside of the liposome in proportion to the level of the antigen-antibody reaction. Then, the amount of the substance being inspected in the sample is measured by an analysis method corresponding to the labeled substance. This improves the stability of holding the labeled substance thereby to achieve a higher measuring sensitivity.
JP62216796A 1987-08-31 1987-08-31 Immunoassay method and immunoassay reagent used therefor Expired - Lifetime JP2553360B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62216796A JP2553360B2 (en) 1987-08-31 1987-08-31 Immunoassay method and immunoassay reagent used therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62216796A JP2553360B2 (en) 1987-08-31 1987-08-31 Immunoassay method and immunoassay reagent used therefor

Publications (2)

Publication Number Publication Date
JPS6459157A true JPS6459157A (en) 1989-03-06
JP2553360B2 JP2553360B2 (en) 1996-11-13

Family

ID=16694011

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62216796A Expired - Lifetime JP2553360B2 (en) 1987-08-31 1987-08-31 Immunoassay method and immunoassay reagent used therefor

Country Status (1)

Country Link
JP (1) JP2553360B2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50100225A (en) * 1974-01-14 1975-08-08
JPS61225190A (en) * 1984-12-28 1986-10-06 テクニコン、インストルメンツ、コ−ポレ−シヨン Phospholipid complex and manufacture
JPS61250559A (en) * 1985-04-30 1986-11-07 Toshiba Corp Immunological assaying method
JPS63106561A (en) * 1986-05-31 1988-05-11 Toshiba Corp Reagent for immunological analysis
JPS63118658A (en) * 1986-11-07 1988-05-23 Toshiba Corp Method and reagent for immunological analysis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50100225A (en) * 1974-01-14 1975-08-08
JPS61225190A (en) * 1984-12-28 1986-10-06 テクニコン、インストルメンツ、コ−ポレ−シヨン Phospholipid complex and manufacture
JPS61250559A (en) * 1985-04-30 1986-11-07 Toshiba Corp Immunological assaying method
JPS63106561A (en) * 1986-05-31 1988-05-11 Toshiba Corp Reagent for immunological analysis
JPS63118658A (en) * 1986-11-07 1988-05-23 Toshiba Corp Method and reagent for immunological analysis

Also Published As

Publication number Publication date
JP2553360B2 (en) 1996-11-13

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