JPS5767858A - Immunochemical measurement method for human chorionic gonadotropin and production of antibody - Google Patents

Immunochemical measurement method for human chorionic gonadotropin and production of antibody

Info

Publication number
JPS5767858A
JPS5767858A JP14468980A JP14468980A JPS5767858A JP S5767858 A JPS5767858 A JP S5767858A JP 14468980 A JP14468980 A JP 14468980A JP 14468980 A JP14468980 A JP 14468980A JP S5767858 A JPS5767858 A JP S5767858A
Authority
JP
Japan
Prior art keywords
antibody
hcg
obtd
marked
accurately
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14468980A
Other languages
Japanese (ja)
Other versions
JPH0136065B2 (en
Inventor
Koichi Kondo
Susumu Iwasa
Isamu Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to JP14468980A priority Critical patent/JPS5767858A/en
Priority to EP81108272A priority patent/EP0049898B2/en
Priority to DE8181108272T priority patent/DE3174064D1/en
Priority to CA000387873A priority patent/CA1181342A/en
Priority to US06/311,327 priority patent/US4496658A/en
Publication of JPS5767858A publication Critical patent/JPS5767858A/en
Publication of JPH0136065B2 publication Critical patent/JPH0136065B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Abstract

PURPOSE:To measure human chorionic gonadotropin (hCG) accurately with high sensitivity by a sandwich method by producing the antibody reacting specifically with the hCG out of the antibodies held on a carrier and marked antibodies by a specific method and using the same. CONSTITUTION:Reptide consisting of about 30 members of amino acids at the C terminal of the hCG-beta expressed by the formula is synthesized by a conventional method. The antibody which is obtd. by inoculating said peptide and protein for a carrir (bovine thyroglobulin, etc.), which are condensated in the presence of glutaraldehyde, in a warm-blooded animal (a rabbit or the like) and reacts specifically with the hCG is obtained. Such antibody is deposited on polyestyrene particles. On the other hand, the antibody consisting of marking the antibody obtd. by immunizing the hCG obtd. from human urine in an eel with beta-D-galactosidase, etc. is obtd. The deposited antibody, the marked antibody, and the hCG in the specimen (bodily fluid such as serum) are caused to react, and the activity of the mark bound with the carrier or the activity of the mark in the unreacted marked antibody is measured. It is measured accurately without disturbance of other protein and the like causing cross reaction with the hCG, and the diagnosis of pregnancy and the like are accomplished accurately.
JP14468980A 1980-10-15 1980-10-15 Immunochemical measurement method for human chorionic gonadotropin and production of antibody Granted JPS5767858A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP14468980A JPS5767858A (en) 1980-10-15 1980-10-15 Immunochemical measurement method for human chorionic gonadotropin and production of antibody
EP81108272A EP0049898B2 (en) 1980-10-15 1981-10-13 Method for immunochemical assay and kit therefor
DE8181108272T DE3174064D1 (en) 1980-10-15 1981-10-13 Method for immunochemical assay and kit therefor
CA000387873A CA1181342A (en) 1980-10-15 1981-10-14 Method for enzyme immunoassay and production of antibody
US06/311,327 US4496658A (en) 1980-10-15 1981-10-14 Method for enzyme immunoassay and production of antibody

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14468980A JPS5767858A (en) 1980-10-15 1980-10-15 Immunochemical measurement method for human chorionic gonadotropin and production of antibody

Publications (2)

Publication Number Publication Date
JPS5767858A true JPS5767858A (en) 1982-04-24
JPH0136065B2 JPH0136065B2 (en) 1989-07-28

Family

ID=15367964

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14468980A Granted JPS5767858A (en) 1980-10-15 1980-10-15 Immunochemical measurement method for human chorionic gonadotropin and production of antibody

Country Status (1)

Country Link
JP (1) JPS5767858A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6117065A (en) * 1984-07-04 1986-01-25 Mitsui Toatsu Chem Inc Measuring reagent for human chorionic gonadotropin
JPS61501164A (en) * 1984-01-13 1986-06-12 セントコア，インコ−ポレ−テツド Immunoassay of biologically active human interferon gamma
JPS63231266A (en) * 1987-03-02 1988-09-27 ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Device for executing heterogeneous system reaction for measuring component of sample liquid and fixing type capillary check valve
WO1992006377A1 (en) * 1990-10-04 1992-04-16 Teijin Limited Method and kit for immunoassay of propeptide of osteocalcin and proosteocalcin
JPH05281225A (en) * 1991-08-26 1993-10-29 Merck & Co Inc Assay for evaluating inhibition of pmn elastase due to n-substituted azetidinone
US7332350B2 (en) 2000-08-12 2008-02-19 Humasis Co., Ltd. Diagnostic device for distinguishing between normal and ectopic pregnancy and method for preparing the same
CN114736285A (en) * 2022-05-18 2022-07-12 江苏尤里卡生物科技有限公司 Preparation device and preparation method of human chorionic gonadotropin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5716355A (en) * 1980-04-25 1982-01-27 Hoffmann La Roche Immunological method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5716355A (en) * 1980-04-25 1982-01-27 Hoffmann La Roche Immunological method

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61501164A (en) * 1984-01-13 1986-06-12 セントコア，インコ−ポレ−テツド Immunoassay of biologically active human interferon gamma
JPS6117065A (en) * 1984-07-04 1986-01-25 Mitsui Toatsu Chem Inc Measuring reagent for human chorionic gonadotropin
JPS63231266A (en) * 1987-03-02 1988-09-27 ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Device for executing heterogeneous system reaction for measuring component of sample liquid and fixing type capillary check valve
WO1992006377A1 (en) * 1990-10-04 1992-04-16 Teijin Limited Method and kit for immunoassay of propeptide of osteocalcin and proosteocalcin
JPH05281225A (en) * 1991-08-26 1993-10-29 Merck & Co Inc Assay for evaluating inhibition of pmn elastase due to n-substituted azetidinone
US7332350B2 (en) 2000-08-12 2008-02-19 Humasis Co., Ltd. Diagnostic device for distinguishing between normal and ectopic pregnancy and method for preparing the same
CN114736285A (en) * 2022-05-18 2022-07-12 江苏尤里卡生物科技有限公司 Preparation device and preparation method of human chorionic gonadotropin
CN114736285B (en) * 2022-05-18 2023-11-03 江苏尤里卡生物科技有限公司 Device and method for preparing human chorionic gonadotrophin

Also Published As

Publication number Publication date
JPH0136065B2 (en) 1989-07-28

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