JPS56118671A - Measuring method of specific enzyme immunity of hybrid type protein - Google Patents

Measuring method of specific enzyme immunity of hybrid type protein

Info

Publication number
JPS56118671A
JPS56118671A JP2049180A JP2049180A JPS56118671A JP S56118671 A JPS56118671 A JP S56118671A JP 2049180 A JP2049180 A JP 2049180A JP 2049180 A JP2049180 A JP 2049180A JP S56118671 A JPS56118671 A JP S56118671A
Authority
JP
Japan
Prior art keywords
antibody
bonded
component
hybrid type
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2049180A
Other languages
Japanese (ja)
Other versions
JPS6223823B2 (en
Inventor
Kanefusa Kato
Akira Kosaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP2049180A priority Critical patent/JPS56118671A/en
Publication of JPS56118671A publication Critical patent/JPS56118671A/en
Publication of JPS6223823B2 publication Critical patent/JPS6223823B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Rehabilitation Therapy (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To measure enzyme immunity precisely, in measuring the hybrid type proteins A.B, by reacting the insoluble antibody wherein the antibody having a component A is bonded in a solid phase with the antibody having a component B wherein the protein A.B is bonded by antigen-antibody reaction and then labeling enzyme is bonded. CONSTITUTION:The amount of presence of the aggregate A.B of the immunologically different preteins A and B in the mixture of aggregates A.A, B.B, and A.B is measured. In this case, the antibody 2 having the component A is bonded to a solid-phase carrier 1. A specimen is added in the mixture, antigen-antibody reaction is carried out, and the bonded body of the insoluble antibody 2 and the protein A.B is formed. Then, the antibody having the component B is reacted with the labeled antibody 3 to which the labeling enzyme is bonded. By measuring the activity of the insoluble antibody 2 and the labeling enzyme of the labeled antibody 3 which is bonded with the aggregate A.B, very small amount of the hybrid type protein A.B in the mixture can be measured precisely. By this method, metabolic phenomena and diseases can be accuretely diagnosed.
JP2049180A 1980-02-22 1980-02-22 Measuring method of specific enzyme immunity of hybrid type protein Granted JPS56118671A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2049180A JPS56118671A (en) 1980-02-22 1980-02-22 Measuring method of specific enzyme immunity of hybrid type protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2049180A JPS56118671A (en) 1980-02-22 1980-02-22 Measuring method of specific enzyme immunity of hybrid type protein

Publications (2)

Publication Number Publication Date
JPS56118671A true JPS56118671A (en) 1981-09-17
JPS6223823B2 JPS6223823B2 (en) 1987-05-25

Family

ID=12028613

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2049180A Granted JPS56118671A (en) 1980-02-22 1980-02-22 Measuring method of specific enzyme immunity of hybrid type protein

Country Status (1)

Country Link
JP (1) JPS56118671A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57551A (en) * 1980-04-30 1982-01-05 Merck Patent Gmbh Immunologic measuring method and means of enzyme
JPS5740653A (en) * 1980-06-30 1982-03-06 Int Imiyunoasei Lab Inc Sandwitch analysis of measuring 2-point crossing reaction immunity
JPS5794660A (en) * 1980-09-30 1982-06-12 Behringwerke Ag Measurement of inhibitor-enzyme compound
JPS5899758A (en) * 1981-12-09 1983-06-14 Amano Pharmaceut Co Ltd Determination of secretary component
JPS58198758A (en) * 1982-05-15 1983-11-18 Amano Pharmaceut Co Ltd Method for determining enolase and diagnosing cancer using it
JPS59111062A (en) * 1982-12-16 1984-06-27 Amano Pharmaceut Co Ltd Measurement of beta-enolase
US4515890A (en) * 1982-11-08 1985-05-07 Abbott Laboratories Immunoassay of terminal deoxynucleotidyl transferase
JPH026755A (en) * 1988-01-19 1990-01-10 Inst Pasteur Immunological measurement of lipoprotein particle and anti lp (a) monoclonal antibody and use thereof for diagnosis of atherosclerosis

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57551A (en) * 1980-04-30 1982-01-05 Merck Patent Gmbh Immunologic measuring method and means of enzyme
JPH0370184B2 (en) * 1980-04-30 1991-11-06 Merck Patent Gmbh
JPS5740653A (en) * 1980-06-30 1982-03-06 Int Imiyunoasei Lab Inc Sandwitch analysis of measuring 2-point crossing reaction immunity
JPS5794660A (en) * 1980-09-30 1982-06-12 Behringwerke Ag Measurement of inhibitor-enzyme compound
JPH041304B2 (en) * 1980-09-30 1992-01-10 Kooneru Risaachi Fuaundeeshon Inc
JPS5899758A (en) * 1981-12-09 1983-06-14 Amano Pharmaceut Co Ltd Determination of secretary component
JPS58198758A (en) * 1982-05-15 1983-11-18 Amano Pharmaceut Co Ltd Method for determining enolase and diagnosing cancer using it
JPH038515B2 (en) * 1982-05-15 1991-02-06 Amano Pharma Co Ltd
US4515890A (en) * 1982-11-08 1985-05-07 Abbott Laboratories Immunoassay of terminal deoxynucleotidyl transferase
JPS59111062A (en) * 1982-12-16 1984-06-27 Amano Pharmaceut Co Ltd Measurement of beta-enolase
JPH026755A (en) * 1988-01-19 1990-01-10 Inst Pasteur Immunological measurement of lipoprotein particle and anti lp (a) monoclonal antibody and use thereof for diagnosis of atherosclerosis

Also Published As

Publication number Publication date
JPS6223823B2 (en) 1987-05-25

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