JPS6413459A - Enzyme labeled thronboxane b2 and method for measuring 11-dehydro-thromboxane b2 - Google Patents

Enzyme labeled thronboxane b2 and method for measuring 11-dehydro-thromboxane b2

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Publication number
JPS6413459A
JPS6413459A JP16880387A JP16880387A JPS6413459A JP S6413459 A JPS6413459 A JP S6413459A JP 16880387 A JP16880387 A JP 16880387A JP 16880387 A JP16880387 A JP 16880387A JP S6413459 A JPS6413459 A JP S6413459A
Authority
JP
Japan
Prior art keywords
formula
enzyme
dehydro
expressed
txb2
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP16880387A
Other languages
Japanese (ja)
Other versions
JP2587239B2 (en
Inventor
Masabumi Sawada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ono Pharmaceutical Co Ltd
Original Assignee
Ono Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ono Pharmaceutical Co Ltd filed Critical Ono Pharmaceutical Co Ltd
Priority to JP16880387A priority Critical patent/JP2587239B2/en
Publication of JPS6413459A publication Critical patent/JPS6413459A/en
Application granted granted Critical
Publication of JP2587239B2 publication Critical patent/JP2587239B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)

Abstract

PURPOSE:To measure 11-dehydro-thromboxane B2(TXB2) with high accuracy by using specific enzyme labeled thromboxanes B2 and making an analysis by using a two-antibody method for enzyme immunoassay. CONSTITUTION:The enzyme labeled thromboxanes B2 expressed by formula I are use. In formula I, the part of formula II is of the structural formula expressed by formula IV; R<1> denotes enzyme conjugated after one of the hydrogen atoms of the amino group is removed. Said R<1> is, prescribed beta-D- galactosidase, etc. The antigen-antibody complex formed by incubating the specimen, the anti-serum and the enzyme labeled antigen expressed by formula I is separated to a face type and a bond type by the two-antibody method for the enzyme immunoassay. An enzyme substrate is thereafter acted on the antigen-antibody complex and the fluorescent intensity of the product is measured, by which the concn. of the 11-dehydro-TXB2 is determined. Since the analysis is made by using the enzyme label expressed by formula I and by the two-antibody method, the 11-dehydro-TXB2 is measured with the high accuracy.
JP16880387A 1987-07-08 1987-07-08 Enzyme-labeled thromboxane B (2) and method for measuring 11-dehydro-thromboxane B (2) using the same Expired - Lifetime JP2587239B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16880387A JP2587239B2 (en) 1987-07-08 1987-07-08 Enzyme-labeled thromboxane B (2) and method for measuring 11-dehydro-thromboxane B (2) using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16880387A JP2587239B2 (en) 1987-07-08 1987-07-08 Enzyme-labeled thromboxane B (2) and method for measuring 11-dehydro-thromboxane B (2) using the same

Publications (2)

Publication Number Publication Date
JPS6413459A true JPS6413459A (en) 1989-01-18
JP2587239B2 JP2587239B2 (en) 1997-03-05

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ID=15874775

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Application Number Title Priority Date Filing Date
JP16880387A Expired - Lifetime JP2587239B2 (en) 1987-07-08 1987-07-08 Enzyme-labeled thromboxane B (2) and method for measuring 11-dehydro-thromboxane B (2) using the same

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JP (1) JP2587239B2 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003081236A3 (en) * 2002-03-24 2004-04-29 Univ Mcmaster Method and device for predicting cardiovascular events
US6994983B2 (en) * 1999-10-25 2006-02-07 Esoterix, Inc. Coagulation Kits for determination of thromboxane B2 metabolite and optimizing aspirin dosage
CN103941007A (en) * 2014-03-28 2014-07-23 瑞莱生物科技（江苏）有限公司 Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip
CN105974106A (en) * 2016-05-04 2016-09-28 山东盛百灵医药科技有限公司 11-Dehydro-thromboxane B2 determination kit and use thereof
CN111443203A (en) * 2020-02-25 2020-07-24 深圳市安帝宝科技有限公司 11-dehydrothromboxane B2 homogeneous enzyme immunoassay kit and preparation method thereof
CN111443201A (en) * 2020-02-25 2020-07-24 深圳市安帝宝科技有限公司 Aspirin drug resistance detection kit
CN115656400A (en) * 2022-11-09 2023-01-31 大连博源医学科技有限公司 Method for detecting 11-dehydrothromboxane B in urine 2 Liquid chromatography-tandem mass spectrometry method and kit

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994983B2 (en) * 1999-10-25 2006-02-07 Esoterix, Inc. Coagulation Kits for determination of thromboxane B2 metabolite and optimizing aspirin dosage
WO2003081236A3 (en) * 2002-03-24 2004-04-29 Univ Mcmaster Method and device for predicting cardiovascular events
US7081347B2 (en) 2002-03-24 2006-07-25 Mcmaster University Method for predicting cardiovascular events
US7595201B2 (en) 2002-03-24 2009-09-29 Mcmaster University Device for measuring an analyte
CN103941007A (en) * 2014-03-28 2014-07-23 瑞莱生物科技（江苏）有限公司 Immunofluorescence test strip for fast and quantitatively detecting curative effect of aspirin and preparation method of immunofluorescence test strip
CN105974106A (en) * 2016-05-04 2016-09-28 山东盛百灵医药科技有限公司 11-Dehydro-thromboxane B2 determination kit and use thereof
CN111443203A (en) * 2020-02-25 2020-07-24 深圳市安帝宝科技有限公司 11-dehydrothromboxane B2 homogeneous enzyme immunoassay kit and preparation method thereof
CN111443201A (en) * 2020-02-25 2020-07-24 深圳市安帝宝科技有限公司 Aspirin drug resistance detection kit
CN115656400A (en) * 2022-11-09 2023-01-31 大连博源医学科技有限公司 Method for detecting 11-dehydrothromboxane B in urine 2 Liquid chromatography-tandem mass spectrometry method and kit
CN115656400B (en) * 2022-11-09 2023-08-18 大连博源医学科技有限公司 Be used for detecting 11-dehydrothromboxane B in urine 2 Liquid chromatography-tandem mass spectrometry method and kit

Also Published As

Publication number Publication date
JP2587239B2 (en) 1997-03-05

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