SE7610683L - METHOD OF DETERMINING THE NERVARON OF AN ANTIGEN ASSOCIATE WITH HEPATITIS - Google Patents

METHOD OF DETERMINING THE NERVARON OF AN ANTIGEN ASSOCIATE WITH HEPATITIS

Info

Publication number
SE7610683L
SE7610683L SE7610683A SE7610683A SE7610683L SE 7610683 L SE7610683 L SE 7610683L SE 7610683 A SE7610683 A SE 7610683A SE 7610683 A SE7610683 A SE 7610683A SE 7610683 L SE7610683 L SE 7610683L
Authority
SE
Sweden
Prior art keywords
antigen
antibody
enzyme
incubation
hepatitis
Prior art date
Application number
SE7610683A
Other languages
Unknown language ( )
Swedish (sv)
Inventor
S P Halbert
M Anken
Original Assignee
Cordis Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/617,745 external-priority patent/US4157280A/en
Priority claimed from US06/617,743 external-priority patent/US4474878A/en
Application filed by Cordis Corp filed Critical Cordis Corp
Publication of SE7610683L publication Critical patent/SE7610683L/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Communicable Diseases (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A sensitive direct immunoassay system is provided for the detection of an antigen associated with hepatitis in body fluids. An antibody which reacts with a hepatitis antigen or antigens and which is uniformly bonded to an insoluble member comprising a polymeric material having irregular surfaces, is incubated with a test sample. During this first incubation, a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent comprising immunochemically reactive hepatitis antibody conjugated with a functional enzyme such as alkaline phosphatase. During the second incubation, the tagged antibody reacts with antigen fixed to the insoluble member in the first incubation. Thus, a "sandwich" is formed of an insoluble member- antibodyantigen- enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme-antibody conjugate reagent. The member is then exposed to a substrate which is induced by the now immobilized enzyme to produce a detectable end product. The enzyme-antibody conjugate will be fixed in the second incubation only if antigen was present in the sample. The amount of enzyme tagged antibody fixed is proportional to the amount of antigen or antigens present in the test sample up to the maximum capacity of the test. The concentration of the end product, and hence the amount of antigen or antigens, is determined by a spectrophotometer which measures the optical absorption of light by the end product. This readout is then compared against a standard value for both antigen negative and antigen positive samples. All reagents for the test may be conveniently furnished in the form of a kit which has an extended shelf life and greatly simplifies the procedure.
SE7610683A 1975-09-29 1976-09-27 METHOD OF DETERMINING THE NERVARON OF AN ANTIGEN ASSOCIATE WITH HEPATITIS SE7610683L (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61774675A 1975-09-29 1975-09-29
US61774475A 1975-09-29 1975-09-29
US05/617,745 US4157280A (en) 1975-09-29 1975-09-29 Test set for detecting the presence of antigens associated with hepatitis
US06/617,743 US4474878A (en) 1975-09-29 1975-09-29 Sandwich EIA for antigen associated with hepatitis

Publications (1)

Publication Number Publication Date
SE7610683L true SE7610683L (en) 1977-06-10

Family

ID=27505141

Family Applications (1)

Application Number Title Priority Date Filing Date
SE7610683A SE7610683L (en) 1975-09-29 1976-09-27 METHOD OF DETERMINING THE NERVARON OF AN ANTIGEN ASSOCIATE WITH HEPATITIS

Country Status (7)

Country Link
JP (1) JPS5257316A (en)
CA (1) CA1106281A (en)
DE (1) DE2643829A1 (en)
FR (1) FR2325934A1 (en)
GB (1) GB1571196A (en)
NL (1) NL7610801A (en)
SE (1) SE7610683L (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU527489B2 (en) * 1978-03-20 1983-03-10 Abbott Laboratories Sugar coated reagents for solid phase immunoassay
US4241175A (en) * 1978-12-18 1980-12-23 Merck & Co., Inc. Assay for hepatitis B core antibody
CA1148859A (en) * 1979-06-14 1983-06-28 Lacy R. Overby Simultaneous assay of two hepatitis viruses using a solid phase
EP0139316B1 (en) * 1983-09-14 1989-04-05 Akzo N.V. Method for the immunochemical determination of hepatitis b core antigens
JPH0318011U (en) * 1989-07-05 1991-02-22
JP3058673B2 (en) * 1989-11-10 2000-07-04 株式会社明電舎 Method for measuring cytokine and kit for measuring the same
KR101799826B1 (en) * 2016-09-19 2017-11-21 바이오뱅크 주식회사 Multi-unit for conducting biochemistry and immunoassey analysis and testing-Method using thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (en) * 1971-02-10 1977-09-15 Organon Nv METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES.
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
NL154599B (en) * 1970-12-28 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING.
US3867517A (en) * 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
US3826619A (en) * 1971-12-21 1974-07-30 Abbott Lab Test apparatus for direct radioimmuno-assay for antigens and their antibodies
US4039652A (en) * 1973-10-11 1977-08-02 Miles Laboratories, Inc. Column method of immunoassay employing an immobilized binding partner
US4034072A (en) * 1975-07-21 1977-07-05 Corning Glass Works Serum hepatitis test

Also Published As

Publication number Publication date
GB1571196A (en) 1980-07-09
DE2643829A1 (en) 1977-04-14
JPS5257316A (en) 1977-05-11
JPS6224745B2 (en) 1987-05-29
CA1106281A (en) 1981-08-04
FR2325934B1 (en) 1982-08-20
FR2325934A1 (en) 1977-04-22
NL7610801A (en) 1977-03-31

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