DE2515966C3 - Vorgefärbte Objektträger für die Blutunte rsuchung - Google Patents
Vorgefärbte Objektträger für die Blutunte rsuchungInfo
- Publication number
- DE2515966C3 DE2515966C3 DE2515966A DE2515966A DE2515966C3 DE 2515966 C3 DE2515966 C3 DE 2515966C3 DE 2515966 A DE2515966 A DE 2515966A DE 2515966 A DE2515966 A DE 2515966A DE 2515966 C3 DE2515966 C3 DE 2515966C3
- Authority
- DE
- Germany
- Prior art keywords
- dyes
- methylene blue
- blood
- stained
- blood testing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004369 blood Anatomy 0.000 title claims description 10
- 239000008280 blood Substances 0.000 title claims description 10
- 238000012360 testing method Methods 0.000 title claims description 4
- 239000000975 dye Substances 0.000 claims description 28
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- SQHOAFZGYFNDQX-UHFFFAOYSA-N ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride Chemical compound [Cl-].S1C2=CC(=[NH+]CC)C(C)=CC2=NC2=C1C=C(NCC)C(C)=C2 SQHOAFZGYFNDQX-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 8
- VHGXRGXCDVQIKS-KRWDZBQOSA-N methyl (2s)-3-(4-methylphenyl)sulfonyloxy-2-(phenylmethoxycarbonylamino)propanoate Chemical compound C([C@@H](C(=O)OC)NC(=O)OCC=1C=CC=CC=1)OS(=O)(=O)C1=CC=C(C)C=C1 VHGXRGXCDVQIKS-KRWDZBQOSA-N 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000009534 blood test Methods 0.000 claims 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000004043 dyeing Methods 0.000 description 5
- 239000013078 crystal Substances 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical group [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 4
- 210000001772 blood platelet Anatomy 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 210000003979 eosinophil Anatomy 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- ZHAFUINZIZIXFC-UHFFFAOYSA-N [9-(dimethylamino)-10-methylbenzo[a]phenoxazin-5-ylidene]azanium;chloride Chemical compound [Cl-].O1C2=CC(=[NH2+])C3=CC=CC=C3C2=NC2=C1C=C(N(C)C)C(C)=C2 ZHAFUINZIZIXFC-UHFFFAOYSA-N 0.000 description 2
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 2
- 210000003651 basophil Anatomy 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- IJKVHSBPTUYDLN-UHFFFAOYSA-N dihydroxy(oxo)silane Chemical compound O[Si](O)=O IJKVHSBPTUYDLN-UHFFFAOYSA-N 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 229960000907 methylthioninium chloride Drugs 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 210000000440 neutrophil Anatomy 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 210000001995 reticulocyte Anatomy 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- 235000005074 zinc chloride Nutrition 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- RFQSMLBZXQOMKK-UHFFFAOYSA-N [3-[(4,8-diamino-6-bromo-1,5-dioxonaphthalen-2-yl)amino]phenyl]-trimethylazanium;chloride Chemical compound [Cl-].C[N+](C)(C)C1=CC=CC(NC=2C(C3=C(N)C=C(Br)C(=O)C3=C(N)C=2)=O)=C1 RFQSMLBZXQOMKK-UHFFFAOYSA-N 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 125000000853 cresyl group Chemical group C1(=CC=C(C=C1)C)* 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- XJCPMUIIBDVFDM-UHFFFAOYSA-M nile blue A Chemical compound [Cl-].C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4[O+]=C3C=C(N)C2=C1 XJCPMUIIBDVFDM-UHFFFAOYSA-M 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 235000010483 polyoxyethylene sorbitan monopalmitate Nutrition 0.000 description 1
- 239000000249 polyoxyethylene sorbitan monopalmitate Substances 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (24)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2515966A DE2515966C3 (de) | 1975-04-11 | 1975-04-11 | Vorgefärbte Objektträger für die Blutunte rsuchung |
| SE7602728A SE420355B (sv) | 1975-04-11 | 1976-02-27 | I forveg fergad objektplatta for blodundersokning samt fergemnesblandning for framstellning av densamma |
| DK149876A DK145318C (da) | 1975-04-11 | 1976-03-31 | Farvet objektbaerer til blodundersoegelse og middel til brug ved fremstilling heraf |
| US05/672,572 US4070495A (en) | 1975-04-11 | 1976-03-31 | Microscope slide |
| CA249,401A CA1052675A (en) | 1975-04-11 | 1976-04-01 | Pre-coloured microscope slide for blood investigations |
| AU12677/76A AU493604B2 (en) | 1975-04-11 | 1976-04-05 | Improved pre-coloured microscope slide for blood investigations |
| HU76BO1607A HU176928B (hu) | 1975-04-11 | 1976-04-05 | Zarannee okrashennoe predmetnoe steklo dlja issledovanija krovi |
| AR262783A AR210757A1 (es) | 1975-04-11 | 1976-04-05 | Portaobjetos precoloreado mejorado para analisis de sangr |
| FI760911A FI59677C (fi) | 1975-04-11 | 1976-04-05 | Pao foerhand faergade objektglas foer blodundersoekning |
| GB13853/76A GB1480576A (en) | 1975-04-11 | 1976-04-06 | Pre-coloured microscope slide for blood investigations |
| DD192223A DD123628A5 (cg-RX-API-DMAC7.html) | 1975-04-11 | 1976-04-06 | |
| IT22060/76A IT1059617B (it) | 1975-04-11 | 1976-04-07 | Vetrini portoaoggetto precolorati migliorati per l esame del sangue |
| CH439376A CH621629A5 (cg-RX-API-DMAC7.html) | 1975-04-11 | 1976-04-07 | |
| BE165900A BE840454A (fr) | 1975-04-11 | 1976-04-07 | Lames pre-enduites de colorants pour examens hematologiques |
| CS762302A CS205019B2 (en) | 1975-04-11 | 1976-04-07 | Preliminary dyed supporting glass for the blood analysis |
| NL7603628A NL7603628A (nl) | 1975-04-11 | 1976-04-07 | Werkwijze voor de vervaardiging van vooraf gekleurde voorwerpsglaasjes. |
| SU762346753A SU904536A3 (ru) | 1975-04-11 | 1976-04-08 | Предметное стекло дл исследовани крови |
| ES446836A ES446836A1 (es) | 1975-04-11 | 1976-04-09 | Procedimiento para la produccion de portaobjetos previamentetenidos, mejorados, para la investigacion de la sangre. |
| LU74738A LU74738A1 (cg-RX-API-DMAC7.html) | 1975-04-11 | 1976-04-09 | |
| AT262076A AT350190B (de) | 1975-04-11 | 1976-04-09 | Verbesserte vorgefaerbte objekttraeger fuer die blutuntersuchung |
| FR7610487A FR2307271A1 (fr) | 1975-04-11 | 1976-04-09 | Porte-objet precolore pour l'examen du sang |
| IE752/76A IE42552B1 (en) | 1975-04-11 | 1976-04-09 | Improved pre-coloured microscope slide for blood investigations |
| PL1976188674A PL107576B1 (pl) | 1975-04-11 | 1976-04-10 | Wstepnie barwione szkielka przedmiotowe do badania krwi |
| JP51041173A JPS51126893A (en) | 1975-04-11 | 1976-04-12 | Pigment preetreated charge glass for blood inspection and improved blood inspection process using the glass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2515966A DE2515966C3 (de) | 1975-04-11 | 1975-04-11 | Vorgefärbte Objektträger für die Blutunte rsuchung |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2515966A1 DE2515966A1 (de) | 1976-10-21 |
| DE2515966B2 DE2515966B2 (de) | 1979-03-22 |
| DE2515966C3 true DE2515966C3 (de) | 1979-11-22 |
Family
ID=5943661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2515966A Expired DE2515966C3 (de) | 1975-04-11 | 1975-04-11 | Vorgefärbte Objektträger für die Blutunte rsuchung |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US4070495A (cg-RX-API-DMAC7.html) |
| JP (1) | JPS51126893A (cg-RX-API-DMAC7.html) |
| AR (1) | AR210757A1 (cg-RX-API-DMAC7.html) |
| AT (1) | AT350190B (cg-RX-API-DMAC7.html) |
| BE (1) | BE840454A (cg-RX-API-DMAC7.html) |
| CA (1) | CA1052675A (cg-RX-API-DMAC7.html) |
| CH (1) | CH621629A5 (cg-RX-API-DMAC7.html) |
| CS (1) | CS205019B2 (cg-RX-API-DMAC7.html) |
| DD (1) | DD123628A5 (cg-RX-API-DMAC7.html) |
| DE (1) | DE2515966C3 (cg-RX-API-DMAC7.html) |
| DK (1) | DK145318C (cg-RX-API-DMAC7.html) |
| ES (1) | ES446836A1 (cg-RX-API-DMAC7.html) |
| FI (1) | FI59677C (cg-RX-API-DMAC7.html) |
| FR (1) | FR2307271A1 (cg-RX-API-DMAC7.html) |
| GB (1) | GB1480576A (cg-RX-API-DMAC7.html) |
| HU (1) | HU176928B (cg-RX-API-DMAC7.html) |
| IE (1) | IE42552B1 (cg-RX-API-DMAC7.html) |
| IT (1) | IT1059617B (cg-RX-API-DMAC7.html) |
| LU (1) | LU74738A1 (cg-RX-API-DMAC7.html) |
| NL (1) | NL7603628A (cg-RX-API-DMAC7.html) |
| PL (1) | PL107576B1 (cg-RX-API-DMAC7.html) |
| SE (1) | SE420355B (cg-RX-API-DMAC7.html) |
| SU (1) | SU904536A3 (cg-RX-API-DMAC7.html) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2651060C3 (de) * | 1976-11-09 | 1980-06-26 | Boehringer Mannheim Gmbh, 6800 Mannheim | Differential-diagnostische Spermauntersuchung |
| US4193980A (en) * | 1978-01-05 | 1980-03-18 | Corning Glass Works | Dry preparation for reticulocyte staining |
| US4248821A (en) * | 1979-07-25 | 1981-02-03 | Dellen Adrian F Van | Method and device for embedding a specimen for microscopic examination |
| US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
| US4400370A (en) * | 1980-03-12 | 1983-08-23 | Lawrence Kass | Metachromatic dye sorption means for differential determination of leukocytes |
| CA1155041A (en) * | 1980-04-21 | 1983-10-11 | Michael E. Jolley | Fluorescent nucleic acid stains |
| US4500509A (en) * | 1981-03-11 | 1985-02-19 | Lawrence Kass | Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes |
| US4606950A (en) * | 1984-08-31 | 1986-08-19 | Ellen M. Corbet | Method for assembling a floral arrangement |
| US5106744A (en) * | 1990-11-01 | 1992-04-21 | Cytocolor Inc. | Method of staining monocytes and compositions thereof |
| PL2322517T3 (pl) | 2004-09-23 | 2019-09-30 | Wista Laboratories Ltd. | Sposoby syntezy chemicznej i oczyszczania związków diaminofenotiazyniowych, w tym chlorku metylotioniny (MTC) |
| PT2057136E (pt) | 2006-07-11 | 2013-08-28 | Wista Lab Ltd | Processos de síntese e/ou purificação de compostos diaminofenotiazínio |
| EP3700419A4 (en) * | 2017-10-26 | 2021-11-10 | Essenlix Corporation | RAPID TEST OF BLOOD PLATES |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3906120A (en) * | 1970-10-30 | 1975-09-16 | Gen Electric | Method for preparing slides for blood evaluation |
| US3796594A (en) * | 1970-10-30 | 1974-03-12 | Gen Electric | Stain coated slides for differentially staining blood |
| IT966513B (it) * | 1970-10-30 | 1974-02-20 | Gen Electric | Vetrini precolorati per esami del sangue |
| GB1473945A (en) * | 1973-05-24 | 1977-05-18 | Gen Electric | Method for preparing slides for blood evaluation |
-
1975
- 1975-04-11 DE DE2515966A patent/DE2515966C3/de not_active Expired
-
1976
- 1976-02-27 SE SE7602728A patent/SE420355B/xx unknown
- 1976-03-31 DK DK149876A patent/DK145318C/da not_active IP Right Cessation
- 1976-03-31 US US05/672,572 patent/US4070495A/en not_active Expired - Lifetime
- 1976-04-01 CA CA249,401A patent/CA1052675A/en not_active Expired
- 1976-04-05 HU HU76BO1607A patent/HU176928B/hu unknown
- 1976-04-05 FI FI760911A patent/FI59677C/fi not_active IP Right Cessation
- 1976-04-05 AR AR262783A patent/AR210757A1/es active
- 1976-04-06 GB GB13853/76A patent/GB1480576A/en not_active Expired
- 1976-04-06 DD DD192223A patent/DD123628A5/xx unknown
- 1976-04-07 CS CS762302A patent/CS205019B2/cs unknown
- 1976-04-07 CH CH439376A patent/CH621629A5/de not_active IP Right Cessation
- 1976-04-07 BE BE165900A patent/BE840454A/xx not_active IP Right Cessation
- 1976-04-07 NL NL7603628A patent/NL7603628A/xx not_active Application Discontinuation
- 1976-04-07 IT IT22060/76A patent/IT1059617B/it active
- 1976-04-08 SU SU762346753A patent/SU904536A3/ru active
- 1976-04-09 AT AT262076A patent/AT350190B/de not_active IP Right Cessation
- 1976-04-09 ES ES446836A patent/ES446836A1/es not_active Expired
- 1976-04-09 IE IE752/76A patent/IE42552B1/en unknown
- 1976-04-09 LU LU74738A patent/LU74738A1/xx unknown
- 1976-04-09 FR FR7610487A patent/FR2307271A1/fr active Granted
- 1976-04-10 PL PL1976188674A patent/PL107576B1/pl unknown
- 1976-04-12 JP JP51041173A patent/JPS51126893A/ja active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| C3 | Grant after two publication steps (3rd publication) |