DE2113119A1 - Verfahren zur Reinigung von Enzymen - Google Patents
Verfahren zur Reinigung von EnzymenInfo
- Publication number
- DE2113119A1 DE2113119A1 DE19712113119 DE2113119A DE2113119A1 DE 2113119 A1 DE2113119 A1 DE 2113119A1 DE 19712113119 DE19712113119 DE 19712113119 DE 2113119 A DE2113119 A DE 2113119A DE 2113119 A1 DE2113119 A1 DE 2113119A1
- Authority
- DE
- Germany
- Prior art keywords
- asparaginase
- ethyl alcohol
- aqueous
- solution
- glycerine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 20
- 108090000790 Enzymes Proteins 0.000 title claims description 11
- 102000004190 Enzymes Human genes 0.000 title claims description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- 102100025573 1-alkyl-2-acetylglycerophosphocholine esterase Human genes 0.000 claims description 20
- 108010024976 Asparaginase Proteins 0.000 claims description 20
- 235000019441 ethanol Nutrition 0.000 claims description 13
- 235000011187 glycerol Nutrition 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 11
- 238000000746 purification Methods 0.000 claims description 6
- 229960003272 asparaginase Drugs 0.000 claims description 5
- DCXYFEDJOCDNAF-UHFFFAOYSA-M asparaginate Chemical compound [O-]C(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-M 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000218645 Cedrus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- -1 ammonium sulfate Chemical class 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/88—Serratia
- Y10S435/881—Serratia marcescens
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR707010333A FR2081581B1 (enExample) | 1970-03-23 | 1970-03-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2113119A1 true DE2113119A1 (de) | 1971-10-14 |
Family
ID=9052712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712113119 Pending DE2113119A1 (de) | 1970-03-23 | 1971-03-18 | Verfahren zur Reinigung von Enzymen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US3741872A (enExample) |
| CA (1) | CA940071A (enExample) |
| DE (1) | DE2113119A1 (enExample) |
| FR (1) | FR2081581B1 (enExample) |
| GB (1) | GB1339350A (enExample) |
| NL (1) | NL7103453A (enExample) |
| SE (1) | SE371445B (enExample) |
| SU (1) | SU455546A3 (enExample) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH498158A (de) * | 1967-12-27 | 1970-10-31 | Bayer Ag | Verfahren zur Anreicherung von L-Asparaginase |
| US3597323A (en) * | 1968-06-07 | 1971-08-03 | Wadley Res Inst & Blood Bank | Method of purifying l-asparaginase |
-
1970
- 1970-03-23 FR FR707010333A patent/FR2081581B1/fr not_active Expired
-
1971
- 1971-03-12 US US00123870A patent/US3741872A/en not_active Expired - Lifetime
- 1971-03-15 NL NL7103453A patent/NL7103453A/xx unknown
- 1971-03-16 SE SE7103401A patent/SE371445B/xx unknown
- 1971-03-17 CA CA108034A patent/CA940071A/en not_active Expired
- 1971-03-18 DE DE19712113119 patent/DE2113119A1/de active Pending
- 1971-03-22 SU SU1642869A patent/SU455546A3/ru active
- 1971-04-19 GB GB2504471*A patent/GB1339350A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2081581B1 (enExample) | 1973-08-10 |
| FR2081581A1 (enExample) | 1971-12-10 |
| GB1339350A (en) | 1973-12-05 |
| US3741872A (en) | 1973-06-26 |
| CA940071A (en) | 1974-01-15 |
| NL7103453A (enExample) | 1971-09-27 |
| SU455546A3 (ru) | 1974-12-30 |
| SE371445B (enExample) | 1974-11-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |