DE1161658B - Gewinnung des Kallikrein-Inaktivators aus Insulin-Abfaellen - Google Patents
Gewinnung des Kallikrein-Inaktivators aus Insulin-AbfaellenInfo
- Publication number
- DE1161658B DE1161658B DEF35630A DEF0035630A DE1161658B DE 1161658 B DE1161658 B DE 1161658B DE F35630 A DEF35630 A DE F35630A DE F0035630 A DEF0035630 A DE F0035630A DE 1161658 B DE1161658 B DE 1161658B
- Authority
- DE
- Germany
- Prior art keywords
- precipitate
- solution
- inactivator
- kallikrein
- acidic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000937 inactivator Effects 0.000 title claims description 23
- 102000001399 Kallikrein Human genes 0.000 title claims description 17
- 108060005987 Kallikrein Proteins 0.000 title claims description 17
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title claims description 15
- 229940125396 insulin Drugs 0.000 title claims description 8
- 102000004877 Insulin Human genes 0.000 title claims description 7
- 108090001061 Insulin Proteins 0.000 title claims description 7
- 239000002699 waste material Substances 0.000 title claims description 5
- 239000002244 precipitate Substances 0.000 claims description 19
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- 230000002378 acidificating effect Effects 0.000 claims description 8
- 210000000496 pancreas Anatomy 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- 210000004907 gland Anatomy 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 210000003681 parotid gland Anatomy 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 8
- 235000002639 sodium chloride Nutrition 0.000 claims 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 7
- 239000012452 mother liquor Substances 0.000 claims 5
- 239000011780 sodium chloride Substances 0.000 claims 5
- 229910021529 ammonia Inorganic materials 0.000 claims 4
- 238000006386 neutralization reaction Methods 0.000 claims 4
- 229940062190 pancreas extract Drugs 0.000 claims 4
- 238000003756 stirring Methods 0.000 claims 4
- 241000283690 Bos taurus Species 0.000 claims 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 3
- 230000001476 alcoholic effect Effects 0.000 claims 3
- 239000005909 Kieselgur Substances 0.000 claims 2
- 241001465754 Metazoa Species 0.000 claims 2
- 229920001429 chelating resin Polymers 0.000 claims 2
- 230000008021 deposition Effects 0.000 claims 2
- 239000000047 product Substances 0.000 claims 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims 1
- 241001072332 Monia Species 0.000 claims 1
- 241000282887 Suidae Species 0.000 claims 1
- 101710162629 Trypsin inhibitor Proteins 0.000 claims 1
- 229940122618 Trypsin inhibitor Drugs 0.000 claims 1
- 239000013543 active substance Substances 0.000 claims 1
- 239000012675 alcoholic extract Substances 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 235000015278 beef Nutrition 0.000 claims 1
- 239000001110 calcium chloride Substances 0.000 claims 1
- 229910001628 calcium chloride Inorganic materials 0.000 claims 1
- 244000309466 calf Species 0.000 claims 1
- 238000004140 cleaning Methods 0.000 claims 1
- 238000004040 coloring Methods 0.000 claims 1
- 230000003544 deproteinization Effects 0.000 claims 1
- 239000012467 final product Substances 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000011565 manganese chloride Substances 0.000 claims 1
- 235000002867 manganese chloride Nutrition 0.000 claims 1
- 229940099607 manganese chloride Drugs 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000005416 organic matter Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000002753 trypsin inhibitor Substances 0.000 claims 1
- 210000004185 liver Anatomy 0.000 description 5
- 210000001557 animal structure Anatomy 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 210000002751 lymph Anatomy 0.000 description 3
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 2
- 150000001342 alkaline earth metals Chemical class 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 241000282849 Ruminantia Species 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000011260 aqueous acid Substances 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 235000021336 beef liver Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000003022 colostrum Anatomy 0.000 description 1
- 235000021277 colostrum Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000004679 hydroxides Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 229940075930 picrate Drugs 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-M picrate anion Chemical compound [O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-M 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL287049D NL287049A (enrdf_load_stackoverflow) | 1961-12-22 | ||
| DEF35630A DE1161658B (de) | 1961-12-22 | 1961-12-22 | Gewinnung des Kallikrein-Inaktivators aus Insulin-Abfaellen |
| CH1357162A CH449850A (de) | 1961-12-22 | 1962-11-20 | Verfahren zur Herstellung eines Kallikrein-Inaktivators aus Insulin-Abfällen |
| GB4609862A GB965352A (en) | 1961-12-22 | 1962-12-06 | Production of a callikrein inactivator from insulin wastes |
| DK556762A DK103411C (da) | 1961-12-22 | 1962-12-20 | Fremgangsmåde til fremstilling af en kallikrein-inaktivator af pankreas. |
| SE13893/62A SE323170B (enrdf_load_stackoverflow) | 1961-12-22 | 1962-12-21 | |
| FR919513A FR1345316A (fr) | 1961-12-22 | 1962-12-21 | Production d'un inactivateur de la kallicréine à partir de résidus de la production d'insuline |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEF35630A DE1161658B (de) | 1961-12-22 | 1961-12-22 | Gewinnung des Kallikrein-Inaktivators aus Insulin-Abfaellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1161658B true DE1161658B (de) | 1964-01-23 |
Family
ID=7096079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEF35630A Pending DE1161658B (de) | 1961-12-22 | 1961-12-22 | Gewinnung des Kallikrein-Inaktivators aus Insulin-Abfaellen |
Country Status (7)
| Country | Link |
|---|---|
| CH (1) | CH449850A (enrdf_load_stackoverflow) |
| DE (1) | DE1161658B (enrdf_load_stackoverflow) |
| DK (1) | DK103411C (enrdf_load_stackoverflow) |
| FR (1) | FR1345316A (enrdf_load_stackoverflow) |
| GB (1) | GB965352A (enrdf_load_stackoverflow) |
| NL (1) | NL287049A (enrdf_load_stackoverflow) |
| SE (1) | SE323170B (enrdf_load_stackoverflow) |
-
0
- NL NL287049D patent/NL287049A/xx unknown
-
1961
- 1961-12-22 DE DEF35630A patent/DE1161658B/de active Pending
-
1962
- 1962-11-20 CH CH1357162A patent/CH449850A/de unknown
- 1962-12-06 GB GB4609862A patent/GB965352A/en not_active Expired
- 1962-12-20 DK DK556762A patent/DK103411C/da active
- 1962-12-21 SE SE13893/62A patent/SE323170B/xx unknown
- 1962-12-21 FR FR919513A patent/FR1345316A/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| SE323170B (enrdf_load_stackoverflow) | 1970-04-27 |
| GB965352A (en) | 1964-07-29 |
| CH449850A (de) | 1968-01-15 |
| FR1345316A (fr) | 1963-12-06 |
| NL287049A (enrdf_load_stackoverflow) | |
| DK103411C (da) | 1965-12-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE1161658B (de) | Gewinnung des Kallikrein-Inaktivators aus Insulin-Abfaellen | |
| DE1084433B (de) | Verfahren zur Herstellung von reinen Loesungen des Kallikrein-Inaktivators | |
| US3308026A (en) | Process for the production and recovery of the kallikrein-inacti-vator from proteinaceous hormone wastes | |
| DE719026C (de) | Verfahren zur Herstellung von Praeparaten, die das Kreislaufhormon Kallikrein enthalten | |
| AT225349B (de) | Verfahren zur Herstellung von reinen Lösungen des Kallikrein-Inaktivators | |
| DE1037651B (de) | Verfahren zur Herstellung eines therapeutisch wirksamen Extraktes aus dem Kittharz der Apis mellifica | |
| AT200257B (enrdf_load_stackoverflow) | ||
| AT215596B (enrdf_load_stackoverflow) | ||
| DE701602C (de) | Verfahren zur Gewinnung von Prolaktin | |
| DE626274C (de) | Verfahren zur Gewinnung physiologisch wirksamer Substanzen aus Muskeln | |
| DE557663C (de) | Verfahren zur Gewinnung und Trennung der lipoid- und der wasserloeslichen wirksamen Substanzen aus maennlichen und weiblichen Keimdruesen | |
| DE741472C (de) | Verfahren zur Trennung von Hypophysenhormonen | |
| DE1148352B (de) | Verfahren zur Herstellung von reinen Loesungen des Kallikrein-Inaktivators | |
| DE954284C (de) | Verfahren zur Gewinnung eines Kallikrein-Inaktivators | |
| DE1467773A1 (de) | Polypeptid und Verfahren zur Herstellung desselben | |
| DE672856C (de) | Verfahren zur Gewinnung von Hormonen mit Eiweisscharakter | |
| DE480410C (de) | Verfahren zur Darstellung eines Glucosids aus Adonis vernalis L. | |
| DE963183C (de) | Verfahren zur Gewinnung von Geninen aus Glycosiden | |
| DE744638C (de) | Verfahren zur Anreicherung von gonadotropen Hypophysenhormonen | |
| DE658210C (de) | Verfahren zur Darstellung eines blutdrucksteigernden Koerpers | |
| DE558567C (de) | Verfahren zur Herstellung von loeslichen Abkoemmlingen organischer Antimonverbindungen | |
| Cerecedo et al. | The Use of Synthetic Zeolites in the Isolation of Vitamin B1. III. Experiments with Wheat Germ1 | |
| DE2028739C3 (de) | Amylase-Inhibitor | |
| DE859514C (de) | Verfahren zur Gewinnung antianaemischer Wirkstoffe aus Leber | |
| DE1181859B (de) | Verfahren zur Gewinnung eines Trypsin-Inhibitors |