CY1120410T1 - Μεθοδος για την παραγωγη τοξινης αλλαντιασης - Google Patents
Μεθοδος για την παραγωγη τοξινης αλλαντιασηςInfo
- Publication number
- CY1120410T1 CY1120410T1 CY20181100439T CY181100439T CY1120410T1 CY 1120410 T1 CY1120410 T1 CY 1120410T1 CY 20181100439 T CY20181100439 T CY 20181100439T CY 181100439 T CY181100439 T CY 181100439T CY 1120410 T1 CY1120410 T1 CY 1120410T1
- Authority
- CY
- Cyprus
- Prior art keywords
- botulinum toxin
- toxin
- botulinum
- treatment
- precipitate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Η παρούσα εφεύρεση σχετίζεται με μια μέθοδο για την παραγωγή μιας τοξίνης αλλαντίασης και περισσότερο συγκεκριμένα με μια μέθοδο για την παρασκευή τοξίνης αλλαντίασης, όπου η μέθοδος περιλαμβάνει τα στάδια: (α) της επεξεργασίας μιας καλλιέργειας ενός στελέχους παραγωγής τοξίνης αλλαντίασης με οξύ για την κατακρήμνιση μιας τοξίνης αλλαντίασης· (β) της προσθήκης ρυθμιστικού διαλύματος στην κατακρημνισμένη τοξίνη αλλαντίασης και της ακόλουθης επεξεργασίας με έναν αναστολέα πρωτεάσης και νουκλεάση, εκχυλίζοντας έτσι την τοξίνη αλλαντίασης· (γ) της επεξεργασίας της εκχυλισμένης τοξίνης αλλαντίασης με οξύ για την κατακρήμνιση της τοξίνης αλλαντίασης και της διάλυσης του κατακρημνίσματος σε ρυθμιστικό διάλυμα· και (δ) του καθαρισμού της τοξίνης αλλαντίασης με ανιονανταλλακτική χρωματογραφία. Η χρήση της μεθόδου της τοξίνης της εφεύρεσης καθιστά δυνατή την παραγωγή μιας υψηλής καθαρότητας τοξίνης αλλαντίασης με μια απλή διαδικασία, πράγμα το οποίο υποδηλώνει ότι η μέθοδος είναι ιδιαιτέρως οικονομική και αποτελεσματική. Η τοξίνη αλλαντίασης που παράγεται με τη μέθοδο της εφεύρεσης έχει υψηλή καθαρότητα σε σύγκριση με τις τοξίνες αλλαντίασης που παράγονται με συμβατικές μεθόδους, και έτσι έχει αυξημένη δυνατότητα να δρα σε μια τοπική περιοχή. Έτσι, η κυκλοφορία στο σύστημα της τοξίνης αλλαντίασης, που μπορεί να έχει ως αποτέλεσμα παρενέργειες, είναι μειωμένη για να αυξηθεί η ασφάλεια. Αντίστοιχα, η τοξίνη αλλαντίασης της εφεύρεσης μπορεί να χρησιμοποιηθεί για διάφορους σκοπούς, συμπεριλαμβανομένων της αγωγής νευρομυϊκών διαταραχών, αφαίρεσης των ρυτίδων και της αγωγής της σπαστικής ημιπληγίας και της εγκεφαλικής παράλυσης.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130092024A KR101339349B1 (ko) | 2013-08-02 | 2013-08-02 | 보툴리눔 독소의 제조방법 |
| PCT/KR2014/004003 WO2015016462A1 (en) | 2013-08-02 | 2014-05-07 | Method for production of botulinum toxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1120410T1 true CY1120410T1 (el) | 2019-07-10 |
Family
ID=49987856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20181100439T CY1120410T1 (el) | 2013-08-02 | 2018-04-26 | Μεθοδος για την παραγωγη τοξινης αλλαντιασης |
Country Status (26)
| Country | Link |
|---|---|
| US (1) | US9512418B2 (el) |
| EP (1) | EP2920199B1 (el) |
| JP (2) | JP6307150B2 (el) |
| KR (1) | KR101339349B1 (el) |
| CN (1) | CN105392795B (el) |
| AR (1) | AR096541A1 (el) |
| AU (1) | AU2014297167B2 (el) |
| BR (1) | BR112015029973B1 (el) |
| CA (1) | CA2912553C (el) |
| CY (1) | CY1120410T1 (el) |
| DK (1) | DK2920199T3 (el) |
| ES (1) | ES2665288T3 (el) |
| HK (1) | HK1215715A1 (el) |
| HR (1) | HRP20180679T1 (el) |
| HU (1) | HUE036840T2 (el) |
| LT (1) | LT2920199T (el) |
| MX (1) | MX362821B (el) |
| NO (1) | NO2920199T3 (el) |
| PH (1) | PH12015501855B1 (el) |
| PL (1) | PL2920199T3 (el) |
| PT (1) | PT2920199T (el) |
| RU (1) | RU2627159C2 (el) |
| SA (1) | SA515370208B1 (el) |
| SG (1) | SG11201509486VA (el) |
| SI (1) | SI2920199T1 (el) |
| WO (1) | WO2015016462A1 (el) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| KR101723167B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101723168B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101729251B1 (ko) | 2015-04-28 | 2017-04-21 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101853463B1 (ko) * | 2015-09-09 | 2018-06-20 | 휴젤(주) | 보툴리눔 독소의 정제 방법 |
| KR101775682B1 (ko) * | 2015-11-30 | 2017-09-06 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| US11242515B2 (en) * | 2016-05-16 | 2022-02-08 | President And Fellows Of Harvard College | Method for purification and activation of botulinum neurotoxin |
| KR102463881B1 (ko) * | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
| KR101744900B1 (ko) | 2017-01-20 | 2017-06-08 | 주식회사 대웅 | 보툴리눔 독소를 포함하는 안정한 액상 조성물 |
| CN109646673A (zh) * | 2017-10-12 | 2019-04-19 | 秀杰股份公司 | 肉毒杆菌毒素的微结构制剂技术 |
| JP7454507B2 (ja) | 2018-12-21 | 2024-03-22 | 一般財団法人阪大微生物病研究会 | ボツリヌス毒素の製造方法 |
| KR102516204B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 정제방법 |
| KR102447441B1 (ko) * | 2019-04-15 | 2022-09-27 | (주)제테마 | 보툴리눔 독소의 정제방법 |
| TW202339791A (zh) * | 2022-04-01 | 2023-10-16 | 大陸商重慶譽顏製藥有限公司 | 一種肉毒毒素蛋白組合物、製備方法及其應用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030060150A (ko) * | 2002-01-07 | 2003-07-16 | (주)메디톡스 | 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법 |
| RU2206337C1 (ru) * | 2002-10-29 | 2003-06-20 | Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" | Лекарственный препарат для лечения мышечных дистоний и способ его получения |
| US7452697B2 (en) * | 2003-09-25 | 2008-11-18 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
| WO2006096164A1 (en) | 2005-03-03 | 2006-09-14 | Allergan, Inc. | Media for clostridium bacterium and processes for obtaining a clostridial toxin |
| AU2005274822B2 (en) * | 2004-07-26 | 2008-10-30 | Merz Pharma Gmbh & Co. Kgaa | Therapeutic composition with a botulinum neurotoxin |
| KR101025617B1 (ko) | 2008-02-25 | 2011-03-30 | 메덱스젠 주식회사 | 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법 |
| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| PL2490986T3 (pl) * | 2009-10-21 | 2019-03-29 | Revance Therapeutics, Inc. | Sposoby i systemy oczyszczania nieskompleksowanej neurotoksyny botulinowej |
| KR101434558B1 (ko) | 2011-07-21 | 2014-08-27 | (주)메디톡스 | 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여 클로스트리디움 보툴리눔 독소를 생산하는 방법 |
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
-
2013
- 2013-08-02 KR KR1020130092024A patent/KR101339349B1/ko active IP Right Grant
-
2014
- 2014-05-07 SI SI201430702T patent/SI2920199T1/en unknown
- 2014-05-07 NO NO14832966A patent/NO2920199T3/no unknown
- 2014-05-07 MX MX2015016481A patent/MX362821B/es active IP Right Grant
- 2014-05-07 PT PT148329667T patent/PT2920199T/pt unknown
- 2014-05-07 CN CN201480012202.6A patent/CN105392795B/zh active Active
- 2014-05-07 CA CA2912553A patent/CA2912553C/en active Active
- 2014-05-07 DK DK14832966.7T patent/DK2920199T3/en active
- 2014-05-07 WO PCT/KR2014/004003 patent/WO2015016462A1/en active Application Filing
- 2014-05-07 EP EP14832966.7A patent/EP2920199B1/en active Active
- 2014-05-07 BR BR112015029973-3A patent/BR112015029973B1/pt active IP Right Grant
- 2014-05-07 AU AU2014297167A patent/AU2014297167B2/en active Active
- 2014-05-07 LT LTEP14832966.7T patent/LT2920199T/lt unknown
- 2014-05-07 JP JP2016506267A patent/JP6307150B2/ja active Active
- 2014-05-07 SG SG11201509486VA patent/SG11201509486VA/en unknown
- 2014-05-07 HU HUE14832966A patent/HUE036840T2/hu unknown
- 2014-05-07 RU RU2015151186A patent/RU2627159C2/ru active
- 2014-05-07 ES ES14832966.7T patent/ES2665288T3/es active Active
- 2014-05-07 PL PL14832966T patent/PL2920199T3/pl unknown
- 2014-05-07 US US14/654,819 patent/US9512418B2/en active Active
- 2014-06-06 AR ARP140102204A patent/AR096541A1/es active IP Right Grant
-
2015
- 2015-08-24 PH PH12015501855A patent/PH12015501855B1/en unknown
- 2015-11-29 SA SA515370208A patent/SA515370208B1/ar unknown
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2016
- 2016-03-31 HK HK16103705.2A patent/HK1215715A1/zh unknown
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2017
- 2017-03-06 JP JP2017041239A patent/JP2017141237A/ja active Pending
-
2018
- 2018-04-26 CY CY20181100439T patent/CY1120410T1/el unknown
- 2018-04-27 HR HRP20180679TT patent/HRP20180679T1/hr unknown
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