MX362821B - Metodo para la produccion de toxina botulinica. - Google Patents
Metodo para la produccion de toxina botulinica.Info
- Publication number
- MX362821B MX362821B MX2015016481A MX2015016481A MX362821B MX 362821 B MX362821 B MX 362821B MX 2015016481 A MX2015016481 A MX 2015016481A MX 2015016481 A MX2015016481 A MX 2015016481A MX 362821 B MX362821 B MX 362821B
- Authority
- MX
- Mexico
- Prior art keywords
- botulinum toxin
- precipitate
- toxin
- botulinum
- treatment
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a un método para la producción de un toxina botulínica, el método comprende los pasos de: (a) tratamiento de un cultivo de una cepa que produce toxina botulínica con ácido para precipitar la toxina botulínica; (b) adición de un regulador a la toxina botulínica precipitada, seguida por el tratamiento con un inhibidor de proteasa y nucleasa, de este modo se extrae la toxina botulínica; (c) tratamiento de la toxina botulínica extraída con ácido para precipitar la toxina botulínica y disolver el precipitado en el regulador; y (d) purificación de la toxina botulínica mediante cromatografía de intercambio aniónico, caracterizado porque la precipitación ácida de la etapa (c) se realiza agregando ácido sulfúrico o ácido clorhídrico a la toxina botulínica extraída, de modo que la toxina botulínica extraída alcance un pH de 2.5-4.5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130092024A KR101339349B1 (ko) | 2013-08-02 | 2013-08-02 | 보툴리눔 독소의 제조방법 |
| PCT/KR2014/004003 WO2015016462A1 (en) | 2013-08-02 | 2014-05-07 | Method for production of botulinum toxin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015016481A MX2015016481A (es) | 2016-06-10 |
| MX362821B true MX362821B (es) | 2019-02-18 |
Family
ID=49987856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015016481A MX362821B (es) | 2013-08-02 | 2014-05-07 | Metodo para la produccion de toxina botulinica. |
Country Status (26)
| Country | Link |
|---|---|
| US (1) | US9512418B2 (es) |
| EP (1) | EP2920199B1 (es) |
| JP (2) | JP6307150B2 (es) |
| KR (1) | KR101339349B1 (es) |
| CN (1) | CN105392795B (es) |
| AR (1) | AR096541A1 (es) |
| AU (1) | AU2014297167B2 (es) |
| BR (1) | BR112015029973B1 (es) |
| CA (1) | CA2912553C (es) |
| CY (1) | CY1120410T1 (es) |
| DK (1) | DK2920199T3 (es) |
| ES (1) | ES2665288T3 (es) |
| HK (1) | HK1215715A1 (es) |
| HR (1) | HRP20180679T1 (es) |
| HU (1) | HUE036840T2 (es) |
| LT (1) | LT2920199T (es) |
| MX (1) | MX362821B (es) |
| NO (1) | NO2920199T3 (es) |
| PH (1) | PH12015501855A1 (es) |
| PL (1) | PL2920199T3 (es) |
| PT (1) | PT2920199T (es) |
| RU (1) | RU2627159C2 (es) |
| SA (1) | SA515370208B1 (es) |
| SG (1) | SG11201509486VA (es) |
| SI (1) | SI2920199T1 (es) |
| WO (1) | WO2015016462A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| KR101723168B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101729251B1 (ko) | 2015-04-28 | 2017-04-21 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101723167B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101853463B1 (ko) * | 2015-09-09 | 2018-06-20 | 휴젤(주) | 보툴리눔 독소의 정제 방법 |
| KR101775682B1 (ko) | 2015-11-30 | 2017-09-06 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| WO2017201105A1 (en) * | 2016-05-16 | 2017-11-23 | President And Fellows Of Harvard College | Method for purification and activation of botulinum neurotoxin |
| KR102463881B1 (ko) * | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
| KR101744900B1 (ko) | 2017-01-20 | 2017-06-08 | 주식회사 대웅 | 보툴리눔 독소를 포함하는 안정한 액상 조성물 |
| CN109646673A (zh) * | 2017-10-12 | 2019-04-19 | 秀杰股份公司 | 肉毒杆菌毒素的微结构制剂技术 |
| US11926853B2 (en) | 2018-12-21 | 2024-03-12 | The Research Foundation For Microbial Diseases Of Osaka University | Botulinum toxin producing method |
| KR102516203B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 제조방법 |
| KR102516204B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 정제방법 |
| WO2023186016A1 (zh) * | 2022-04-01 | 2023-10-05 | 重庆誉颜制药有限公司 | 一种肉毒毒素蛋白组合物、制备方法及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030060150A (ko) * | 2002-01-07 | 2003-07-16 | (주)메디톡스 | 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법 |
| RU2206337C1 (ru) * | 2002-10-29 | 2003-06-20 | Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" | Лекарственный препарат для лечения мышечных дистоний и способ его получения |
| US7452697B2 (en) | 2003-09-25 | 2008-11-18 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
| ES2479515T3 (es) * | 2004-07-26 | 2014-07-24 | Merz Pharma Gmbh & Co. Kgaa | Composición terapéutica con una neurotoxina botulínica |
| JP4913074B2 (ja) | 2005-03-03 | 2012-04-11 | アラーガン、インコーポレイテッド | 動物由来産物不含方法およびボツリヌス毒素を精製するための方法 |
| KR101025617B1 (ko) * | 2008-02-25 | 2011-03-30 | 메덱스젠 주식회사 | 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법 |
| US8129139B2 (en) * | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| US20120196349A1 (en) * | 2009-10-21 | 2012-08-02 | Revance Therapeutics, Inc. | Methods and Systems for Purifying Non-Complexed Botulinum Neurotoxin |
| KR101434558B1 (ko) * | 2011-07-21 | 2014-08-27 | (주)메디톡스 | 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여 클로스트리디움 보툴리눔 독소를 생산하는 방법 |
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
-
2013
- 2013-08-02 KR KR1020130092024A patent/KR101339349B1/ko active IP Right Grant
-
2014
- 2014-05-07 HU HUE14832966A patent/HUE036840T2/hu unknown
- 2014-05-07 US US14/654,819 patent/US9512418B2/en active Active
- 2014-05-07 LT LTEP14832966.7T patent/LT2920199T/lt unknown
- 2014-05-07 JP JP2016506267A patent/JP6307150B2/ja active Active
- 2014-05-07 PT PT148329667T patent/PT2920199T/pt unknown
- 2014-05-07 AU AU2014297167A patent/AU2014297167B2/en active Active
- 2014-05-07 PL PL14832966T patent/PL2920199T3/pl unknown
- 2014-05-07 CN CN201480012202.6A patent/CN105392795B/zh active Active
- 2014-05-07 BR BR112015029973-3A patent/BR112015029973B1/pt active IP Right Grant
- 2014-05-07 SG SG11201509486VA patent/SG11201509486VA/en unknown
- 2014-05-07 ES ES14832966.7T patent/ES2665288T3/es active Active
- 2014-05-07 WO PCT/KR2014/004003 patent/WO2015016462A1/en active Application Filing
- 2014-05-07 MX MX2015016481A patent/MX362821B/es active IP Right Grant
- 2014-05-07 EP EP14832966.7A patent/EP2920199B1/en active Active
- 2014-05-07 DK DK14832966.7T patent/DK2920199T3/en active
- 2014-05-07 NO NO14832966A patent/NO2920199T3/no unknown
- 2014-05-07 SI SI201430702T patent/SI2920199T1/en unknown
- 2014-05-07 CA CA2912553A patent/CA2912553C/en active Active
- 2014-05-07 RU RU2015151186A patent/RU2627159C2/ru active
- 2014-06-06 AR ARP140102204A patent/AR096541A1/es active IP Right Grant
-
2015
- 2015-08-24 PH PH12015501855A patent/PH12015501855A1/en unknown
- 2015-11-29 SA SA515370208A patent/SA515370208B1/ar unknown
-
2016
- 2016-03-31 HK HK16103705.2A patent/HK1215715A1/zh unknown
-
2017
- 2017-03-06 JP JP2017041239A patent/JP2017141237A/ja active Pending
-
2018
- 2018-04-26 CY CY20181100439T patent/CY1120410T1/el unknown
- 2018-04-27 HR HRP20180679TT patent/HRP20180679T1/hr unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |