MX362821B - Metodo para la produccion de toxina botulinica. - Google Patents
Metodo para la produccion de toxina botulinica.Info
- Publication number
- MX362821B MX362821B MX2015016481A MX2015016481A MX362821B MX 362821 B MX362821 B MX 362821B MX 2015016481 A MX2015016481 A MX 2015016481A MX 2015016481 A MX2015016481 A MX 2015016481A MX 362821 B MX362821 B MX 362821B
- Authority
- MX
- Mexico
- Prior art keywords
- botulinum toxin
- precipitate
- toxin
- botulinum
- treatment
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente invención se refiere a un método para la producción de un toxina botulínica, el método comprende los pasos de: (a) tratamiento de un cultivo de una cepa que produce toxina botulínica con ácido para precipitar la toxina botulínica; (b) adición de un regulador a la toxina botulínica precipitada, seguida por el tratamiento con un inhibidor de proteasa y nucleasa, de este modo se extrae la toxina botulínica; (c) tratamiento de la toxina botulínica extraída con ácido para precipitar la toxina botulínica y disolver el precipitado en el regulador; y (d) purificación de la toxina botulínica mediante cromatografía de intercambio aniónico, caracterizado porque la precipitación ácida de la etapa (c) se realiza agregando ácido sulfúrico o ácido clorhídrico a la toxina botulínica extraída, de modo que la toxina botulínica extraída alcance un pH de 2.5-4.5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130092024A KR101339349B1 (ko) | 2013-08-02 | 2013-08-02 | 보툴리눔 독소의 제조방법 |
| PCT/KR2014/004003 WO2015016462A1 (en) | 2013-08-02 | 2014-05-07 | Method for production of botulinum toxin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015016481A MX2015016481A (es) | 2016-06-10 |
| MX362821B true MX362821B (es) | 2019-02-18 |
Family
ID=49987856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015016481A MX362821B (es) | 2013-08-02 | 2014-05-07 | Metodo para la produccion de toxina botulinica. |
Country Status (26)
| Country | Link |
|---|---|
| US (1) | US9512418B2 (es) |
| EP (1) | EP2920199B1 (es) |
| JP (2) | JP6307150B2 (es) |
| KR (1) | KR101339349B1 (es) |
| CN (1) | CN105392795B (es) |
| AR (1) | AR096541A1 (es) |
| AU (1) | AU2014297167B2 (es) |
| BR (1) | BR112015029973B1 (es) |
| CA (1) | CA2912553C (es) |
| CY (1) | CY1120410T1 (es) |
| DK (1) | DK2920199T3 (es) |
| ES (1) | ES2665288T3 (es) |
| HK (1) | HK1215715A1 (es) |
| HR (1) | HRP20180679T1 (es) |
| HU (1) | HUE036840T2 (es) |
| LT (1) | LT2920199T (es) |
| MX (1) | MX362821B (es) |
| NO (1) | NO2920199T3 (es) |
| PH (1) | PH12015501855A1 (es) |
| PL (1) | PL2920199T3 (es) |
| PT (1) | PT2920199T (es) |
| RU (1) | RU2627159C2 (es) |
| SA (1) | SA515370208B1 (es) |
| SG (1) | SG11201509486VA (es) |
| SI (1) | SI2920199T1 (es) |
| WO (1) | WO2015016462A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| KR101723168B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101723167B1 (ko) | 2015-04-28 | 2017-04-05 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101729251B1 (ko) | 2015-04-28 | 2017-04-21 | 주식회사 대웅 | 보툴리눔 독소의 제조를 위한 배지 조성물 |
| KR101853463B1 (ko) * | 2015-09-09 | 2018-06-20 | 휴젤(주) | 보툴리눔 독소의 정제 방법 |
| KR101775682B1 (ko) | 2015-11-30 | 2017-09-06 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| EP3458472B1 (en) * | 2016-05-16 | 2021-10-20 | President and Fellows of Harvard College | Method for purification and activation of botulinum neurotoxin |
| KR102463881B1 (ko) | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
| KR101744900B1 (ko) | 2017-01-20 | 2017-06-08 | 주식회사 대웅 | 보툴리눔 독소를 포함하는 안정한 액상 조성물 |
| CN109646673A (zh) * | 2017-10-12 | 2019-04-19 | 秀杰股份公司 | 肉毒杆菌毒素的微结构制剂技术 |
| WO2020129986A1 (ja) * | 2018-12-21 | 2020-06-25 | 一般財団法人阪大微生物病研究会 | ボツリヌス毒素の製造方法 |
| KR102485146B1 (ko) * | 2019-04-15 | 2023-01-06 | (주)제테마 | 보툴리눔 독소의 제조방법 |
| KR102516203B1 (ko) * | 2019-04-15 | 2023-03-30 | (주)제테마 | 보툴리눔 독소의 제조방법 |
| CN118215495A (zh) * | 2022-04-01 | 2024-06-18 | 重庆誉颜制药有限公司 | 一种肉毒毒素蛋白组合物、制备方法及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030060150A (ko) * | 2002-01-07 | 2003-07-16 | (주)메디톡스 | 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법 |
| RU2206337C1 (ru) * | 2002-10-29 | 2003-06-20 | Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" | Лекарственный препарат для лечения мышечных дистоний и способ его получения |
| US7452697B2 (en) * | 2003-09-25 | 2008-11-18 | Allergan, Inc. | Chromatographic method and system for purifying a botulinum toxin |
| ES2479515T3 (es) * | 2004-07-26 | 2014-07-24 | Merz Pharma Gmbh & Co. Kgaa | Composición terapéutica con una neurotoxina botulínica |
| CA2556796C (en) | 2005-03-03 | 2018-01-23 | Allergan, Inc. | Animal product free system and process for purifying a botulinum toxin |
| KR101025617B1 (ko) | 2008-02-25 | 2011-03-30 | 메덱스젠 주식회사 | 클로스트리디움 보툴리눔으로부터 보툴리눔 독소를정제하는 방법 |
| US8129139B2 (en) * | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| MX365496B (es) * | 2009-10-21 | 2019-06-05 | Revance Therapeutics Inc | Metodos y sistemas para purificar la neurotoxina botulinica no acomplejada. |
| KR101434558B1 (ko) | 2011-07-21 | 2014-08-27 | (주)메디톡스 | 식물 유래 성분 함유 배지 및 가요성 폐쇄 용기를 이용하여 클로스트리디움 보툴리눔 독소를 생산하는 방법 |
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
-
2013
- 2013-08-02 KR KR1020130092024A patent/KR101339349B1/ko active IP Right Grant
-
2014
- 2014-05-07 NO NO14832966A patent/NO2920199T3/no unknown
- 2014-05-07 ES ES14832966.7T patent/ES2665288T3/es active Active
- 2014-05-07 HU HUE14832966A patent/HUE036840T2/hu unknown
- 2014-05-07 LT LTEP14832966.7T patent/LT2920199T/lt unknown
- 2014-05-07 AU AU2014297167A patent/AU2014297167B2/en active Active
- 2014-05-07 RU RU2015151186A patent/RU2627159C2/ru active
- 2014-05-07 WO PCT/KR2014/004003 patent/WO2015016462A1/en active Application Filing
- 2014-05-07 US US14/654,819 patent/US9512418B2/en active Active
- 2014-05-07 SI SI201430702T patent/SI2920199T1/en unknown
- 2014-05-07 JP JP2016506267A patent/JP6307150B2/ja active Active
- 2014-05-07 DK DK14832966.7T patent/DK2920199T3/en active
- 2014-05-07 EP EP14832966.7A patent/EP2920199B1/en active Active
- 2014-05-07 CA CA2912553A patent/CA2912553C/en active Active
- 2014-05-07 SG SG11201509486VA patent/SG11201509486VA/en unknown
- 2014-05-07 CN CN201480012202.6A patent/CN105392795B/zh active Active
- 2014-05-07 MX MX2015016481A patent/MX362821B/es active IP Right Grant
- 2014-05-07 PL PL14832966T patent/PL2920199T3/pl unknown
- 2014-05-07 BR BR112015029973-3A patent/BR112015029973B1/pt active IP Right Grant
- 2014-05-07 PT PT148329667T patent/PT2920199T/pt unknown
- 2014-06-06 AR ARP140102204A patent/AR096541A1/es active IP Right Grant
-
2015
- 2015-08-24 PH PH12015501855A patent/PH12015501855A1/en unknown
- 2015-11-29 SA SA515370208A patent/SA515370208B1/ar unknown
-
2016
- 2016-03-31 HK HK16103705.2A patent/HK1215715A1/zh unknown
-
2017
- 2017-03-06 JP JP2017041239A patent/JP2017141237A/ja active Pending
-
2018
- 2018-04-26 CY CY20181100439T patent/CY1120410T1/el unknown
- 2018-04-27 HR HRP20180679TT patent/HRP20180679T1/hr unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |