CO6210760A2 - Secuencias señal y chaperonas co -expresadas para ,emorar la produccion de proteina en una celula hospedera - Google Patents
Secuencias señal y chaperonas co -expresadas para ,emorar la produccion de proteina en una celula hospederaInfo
- Publication number
- CO6210760A2 CO6210760A2 CO10048157A CO10048157A CO6210760A2 CO 6210760 A2 CO6210760 A2 CO 6210760A2 CO 10048157 A CO10048157 A CO 10048157A CO 10048157 A CO10048157 A CO 10048157A CO 6210760 A2 CO6210760 A2 CO 6210760A2
- Authority
- CO
- Colombia
- Prior art keywords
- sequence
- nucleic acid
- enzymatic
- signal
- desired protein
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 7
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 5
- 230000002255 enzymatic effect Effects 0.000 abstract 5
- 150000007523 nucleic acids Chemical group 0.000 abstract 5
- 108010029541 Laccase Proteins 0.000 abstract 2
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 2
- 230000003197 catalytic effect Effects 0.000 abstract 2
- -1 prp1 Proteins 0.000 abstract 2
- 101100407358 Aspergillus niger pdiA gene Proteins 0.000 abstract 1
- 102000034342 Calnexin Human genes 0.000 abstract 1
- 108010056891 Calnexin Proteins 0.000 abstract 1
- 101100046193 Desulfitobacterium hafniense (strain Y51) tig1 gene Proteins 0.000 abstract 1
- 101150047030 ERO1 gene Proteins 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 abstract 1
- 102100022624 Glucoamylase Human genes 0.000 abstract 1
- 108010006519 Molecular Chaperones Proteins 0.000 abstract 1
- 101100084053 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ppi-2 gene Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 101100222355 Schizosaccharomyces pombe (strain 972 / ATCC 24843) cwf2 gene Proteins 0.000 abstract 1
- 108091005749 foldases Proteins 0.000 abstract 1
- 102000035175 foldases Human genes 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 101150031304 ppi1 gene Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03002—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US98443007P | 2007-11-01 | 2007-11-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6210760A2 true CO6210760A2 (es) | 2010-10-20 |
Family
ID=40304979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO10048157A CO6210760A2 (es) | 2007-11-01 | 2010-04-23 | Secuencias señal y chaperonas co -expresadas para ,emorar la produccion de proteina en una celula hospedera |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20090221030A1 (enExample) |
| EP (1) | EP2203556A1 (enExample) |
| JP (3) | JP5252746B2 (enExample) |
| CN (1) | CN101842479A (enExample) |
| AU (1) | AU2008318644B2 (enExample) |
| BR (1) | BRPI0818273A2 (enExample) |
| CA (1) | CA2704548A1 (enExample) |
| CO (1) | CO6210760A2 (enExample) |
| MX (1) | MX2010004325A (enExample) |
| WO (1) | WO2009058956A1 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0924180A8 (pt) | 2008-12-24 | 2017-12-05 | Danisco Us Inc | Lacases e métodos de uso das mesmas em baixa temperatura |
| EP2403809B1 (en) | 2009-03-03 | 2015-06-24 | Danisco US Inc. | Method of oxidative decolorization of dyes with enzymatically generated peracid |
| FI20095615A0 (fi) * | 2009-06-02 | 2009-06-02 | Oulun Yliopisto | Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä |
| AR083471A1 (es) | 2010-10-18 | 2013-02-27 | Danisco Us Inc | Modificacion local del color de telas teñidas empleando un sistema de lacasa |
| US20140123404A1 (en) | 2011-04-06 | 2014-05-08 | Danisco Us Inc. | Laccase variants having increased expression and/or activity |
| JP2012235767A (ja) * | 2011-04-27 | 2012-12-06 | Toyota Motor Corp | 変異トリコデルマ属微生物及びこれを用いたタンパク質の製造方法 |
| RU2645252C2 (ru) | 2012-01-05 | 2018-02-19 | Глюкос Финланд Ой | Клетка нитчатых грибов с дефицитом протеаз и способы ее применения |
| CN104066841B (zh) * | 2012-01-23 | 2018-01-02 | 旭硝子株式会社 | 表达载体和蛋白质的制造方法 |
| EP2852610B1 (en) | 2012-05-23 | 2018-07-11 | Glykos Finland Oy | Production of fucosylated glycoproteins |
| US9993534B2 (en) | 2013-03-12 | 2018-06-12 | Wisconsin Alumni Research Foundation | Method of treating fungal infection |
| SG11201600115SA (en) | 2013-07-10 | 2016-02-26 | Novartis Ag | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
| CN104694560A (zh) * | 2013-12-04 | 2015-06-10 | 中国科学院天津工业生物技术研究所 | 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途 |
| CN106661541B (zh) | 2014-04-17 | 2021-09-03 | 贝林格尔·英格海姆Rcv两合公司 | 被工程化为过表达辅助蛋白的重组宿主细胞 |
| AU2015248807B2 (en) | 2014-04-17 | 2021-07-22 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell for expressing proteins of interest |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| WO2016025825A1 (en) * | 2014-08-15 | 2016-02-18 | Danisco Us Inc | Compositions and methods for improved protein production |
| US10526609B2 (en) * | 2016-08-01 | 2020-01-07 | Aduro Biotech, Inc. | Protein expression enhancer sequences and use thereof |
| JP7645076B2 (ja) * | 2018-06-27 | 2025-03-13 | ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 転写因子の使用によってタンパク質発現を増加させるための手段及び方法 |
| KR102138272B1 (ko) * | 2018-09-19 | 2020-07-28 | 주식회사 바이오앱 | BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법 |
| KR102559048B1 (ko) * | 2019-12-02 | 2023-07-26 | 주식회사 엘지화학 | Cho 세포 유래 단백질 분비 인자 및 이를 포함하는 발현 벡터 |
| CN112501141A (zh) * | 2020-10-22 | 2021-03-16 | 重庆中元汇吉生物技术有限公司 | 一种增加人甲状腺过氧化物酶产量的试剂和表达方法 |
| CN112175977B (zh) * | 2020-10-30 | 2022-04-15 | 华中农业大学 | 一种米曲霉角蛋白酶基因及其表达载体与应用 |
| CN112391402B (zh) * | 2020-11-17 | 2023-03-28 | 华中科技大学 | 一种提高解脂耶氏酵母中目的蛋白表达水平的方法 |
| CN112409464B (zh) * | 2020-11-23 | 2022-04-22 | 江南大学 | 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用 |
| WO2023039358A1 (en) * | 2021-09-09 | 2023-03-16 | Dupont Nutrition Biosciences Aps | Over expression of foldases and chaperones improves protein production |
| CN113736817B (zh) * | 2021-10-08 | 2023-02-03 | 枣庄市杰诺生物酶有限公司 | 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法 |
| KR20240094203A (ko) * | 2022-12-15 | 2024-06-25 | 씨제이제일제당 (주) | 트리코더마 속 균주 유래 pdi를 이용하여 재조합 단백질을 생산하는 방법 |
| CN117903295B (zh) * | 2024-03-19 | 2024-05-31 | 北京国科星联科技有限公司 | 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用 |
| CN117903294B (zh) * | 2024-03-19 | 2024-06-14 | 北京国科星联科技有限公司 | 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2322032T3 (es) * | 1990-12-10 | 2009-06-16 | Genencor Int | Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei. |
| ATE195347T1 (de) * | 1992-10-02 | 2000-08-15 | Res Corp Technologies Inc | Methoden der erhöhung der sekretion von überexpremierten proteinen |
| US5861271A (en) * | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
| US6008029A (en) * | 1995-08-25 | 1999-12-28 | Novo Nordisk Biotech Inc. | Purified coprinus laccases and nucleic acids encoding the same |
| JP4307563B2 (ja) * | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| EP1032655B1 (en) * | 1997-11-21 | 2005-06-29 | Novozymes A/S | Protease variants and compositions |
| US6268328B1 (en) * | 1998-12-18 | 2001-07-31 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| CA2403842C (en) * | 2000-03-24 | 2012-09-25 | Genencor International, Inc. | Increased production of secreted proteins by recombinant eukaryotic cells |
| US6358733B1 (en) * | 2000-05-19 | 2002-03-19 | Apolife, Inc. | Expression of heterologous multi-domain proteins in yeast |
| JP2002065282A (ja) * | 2000-09-04 | 2002-03-05 | Iwate Prefecture | シイタケラッカーゼ遺伝子 |
| ATE482972T1 (de) * | 2003-11-06 | 2010-10-15 | Danisco Us Inc | Tgf-beta1-bindende und geträgerte peptide |
| EP1689766B1 (en) * | 2003-11-06 | 2012-04-25 | Danisco US Inc. | Expression in filamentous fungi of protease inhibitors and variants thereof |
| MXPA06013600A (es) * | 2004-05-27 | 2007-03-15 | Genencor Int | Alfa amilasas acidoestables con actividad hidrolizante de almidon granular y composiciones enzimaticas. |
| EP2363460A3 (en) * | 2004-12-30 | 2011-12-28 | Genencor International, Inc. | Acid fungal proteases |
| EP2092113A2 (en) * | 2006-12-18 | 2009-08-26 | Danisco US, INC., Genencor Division | Laccase mediators and methods of use |
| EP2129685B1 (en) * | 2007-03-21 | 2013-11-20 | Danisco US Inc. | Over expression of foldases and chaperones improves protein production |
-
2008
- 2008-10-30 WO PCT/US2008/081721 patent/WO2009058956A1/en not_active Ceased
- 2008-10-30 BR BRPI0818273-6A2A patent/BRPI0818273A2/pt not_active IP Right Cessation
- 2008-10-30 EP EP08844940A patent/EP2203556A1/en not_active Withdrawn
- 2008-10-30 MX MX2010004325A patent/MX2010004325A/es active IP Right Grant
- 2008-10-30 US US12/261,306 patent/US20090221030A1/en not_active Abandoned
- 2008-10-30 CN CN200880114429A patent/CN101842479A/zh active Pending
- 2008-10-30 JP JP2010532232A patent/JP5252746B2/ja not_active Expired - Fee Related
- 2008-10-30 CA CA2704548A patent/CA2704548A1/en not_active Abandoned
- 2008-10-30 AU AU2008318644A patent/AU2008318644B2/en not_active Ceased
-
2010
- 2010-04-23 CO CO10048157A patent/CO6210760A2/es not_active Application Discontinuation
-
2013
- 2013-01-22 JP JP2013009289A patent/JP2013121354A/ja active Pending
-
2014
- 2014-09-30 JP JP2014200112A patent/JP2015027308A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| MX2010004325A (es) | 2010-05-20 |
| CA2704548A1 (en) | 2009-05-07 |
| JP2013121354A (ja) | 2013-06-20 |
| US20090221030A1 (en) | 2009-09-03 |
| JP2015027308A (ja) | 2015-02-12 |
| EP2203556A1 (en) | 2010-07-07 |
| JP5252746B2 (ja) | 2013-07-31 |
| CN101842479A (zh) | 2010-09-22 |
| AU2008318644A1 (en) | 2009-05-07 |
| BRPI0818273A2 (pt) | 2014-10-14 |
| WO2009058956A1 (en) | 2009-05-07 |
| AU2008318644B2 (en) | 2013-05-02 |
| JP2011502483A (ja) | 2011-01-27 |
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