MX2010004325A - Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera. - Google Patents

Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera.

Info

Publication number
MX2010004325A
MX2010004325A MX2010004325A MX2010004325A MX2010004325A MX 2010004325 A MX2010004325 A MX 2010004325A MX 2010004325 A MX2010004325 A MX 2010004325A MX 2010004325 A MX2010004325 A MX 2010004325A MX 2010004325 A MX2010004325 A MX 2010004325A
Authority
MX
Mexico
Prior art keywords
host cell
sequence
nucleic acid
acid sequence
protein production
Prior art date
Application number
MX2010004325A
Other languages
English (en)
Spanish (es)
Inventor
Huaming Wang
Kai Bao
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2010004325A publication Critical patent/MX2010004325A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
MX2010004325A 2007-11-01 2008-10-30 Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera. MX2010004325A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98443007P 2007-11-01 2007-11-01
PCT/US2008/081721 WO2009058956A1 (en) 2007-11-01 2008-10-30 Signal sequences and co-expressed chaperones for improving protein production in a host cell

Publications (1)

Publication Number Publication Date
MX2010004325A true MX2010004325A (es) 2010-05-20

Family

ID=40304979

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010004325A MX2010004325A (es) 2007-11-01 2008-10-30 Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera.

Country Status (10)

Country Link
US (1) US20090221030A1 (enExample)
EP (1) EP2203556A1 (enExample)
JP (3) JP5252746B2 (enExample)
CN (1) CN101842479A (enExample)
AU (1) AU2008318644B2 (enExample)
BR (1) BRPI0818273A2 (enExample)
CA (1) CA2704548A1 (enExample)
CO (1) CO6210760A2 (enExample)
MX (1) MX2010004325A (enExample)
WO (1) WO2009058956A1 (enExample)

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WO2010075402A1 (en) 2008-12-24 2010-07-01 Danisco Us Inc. Laccases and methods of use thereof at low temperature
PT2403809E (pt) 2009-03-03 2015-10-13 Danisco Us Inc Método de descoloração oxidativa de corantes com perácido gerado por acção enzimática
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
CN103189564A (zh) 2010-10-18 2013-07-03 丹尼斯科美国公司 使用漆酶体系对染色织物进行局部颜色修改
EP2694647A1 (en) 2011-04-06 2014-02-12 Danisco US Inc. Laccase variants having increased expression and/or activity
JP2012235767A (ja) * 2011-04-27 2012-12-06 Toyota Motor Corp 変異トリコデルマ属微生物及びこれを用いたタンパク質の製造方法
US9567596B2 (en) 2012-01-05 2017-02-14 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
EP2808388B1 (en) * 2012-01-23 2019-03-13 AGC Inc. Expression vector and method for producing protein
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
US9993534B2 (en) * 2013-03-12 2018-06-12 Wisconsin Alumni Research Foundation Method of treating fungal infection
JP2016523552A (ja) 2013-07-10 2016-08-12 ノバルティス アーゲー 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
CN104694560A (zh) * 2013-12-04 2015-06-10 中国科学院天津工业生物技术研究所 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途
SG10201809152QA (en) 2014-04-17 2018-11-29 Boehringer Ingelheim Rcv Gmbh Recombinant host cell for expressing proteins of interest
KR102373954B1 (ko) 2014-04-17 2022-03-14 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 보조 단백질을 과발현하도록 조작된 재조합 숙주세포
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
JP2017525359A (ja) * 2014-08-15 2017-09-07 ダニスコ・ユーエス・インク タンパク質の産生改善のための組成物及び方法
US10526609B2 (en) * 2016-08-01 2020-01-07 Aduro Biotech, Inc. Protein expression enhancer sequences and use thereof
WO2020002494A1 (en) * 2018-06-27 2020-01-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of transcription factors
KR102138272B1 (ko) * 2018-09-19 2020-07-28 주식회사 바이오앱 BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법
WO2021112540A1 (ko) * 2019-12-02 2021-06-10 주식회사 엘지화학 Cho 세포 유래 단백질 분비 인자 및 이를 포함하는 발현 벡터
CN112501141A (zh) * 2020-10-22 2021-03-16 重庆中元汇吉生物技术有限公司 一种增加人甲状腺过氧化物酶产量的试剂和表达方法
CN112175977B (zh) * 2020-10-30 2022-04-15 华中农业大学 一种米曲霉角蛋白酶基因及其表达载体与应用
CN112391402B (zh) * 2020-11-17 2023-03-28 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
CN112409464B (zh) * 2020-11-23 2022-04-22 江南大学 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用
EP4399295A1 (en) * 2021-09-09 2024-07-17 International N&H Denmark ApS Over expression of foldases and chaperones improves protein production
CN113736817B (zh) * 2021-10-08 2023-02-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
CN117903294B (zh) * 2024-03-19 2024-06-14 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

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Also Published As

Publication number Publication date
JP2011502483A (ja) 2011-01-27
US20090221030A1 (en) 2009-09-03
JP5252746B2 (ja) 2013-07-31
BRPI0818273A2 (pt) 2014-10-14
AU2008318644A1 (en) 2009-05-07
CN101842479A (zh) 2010-09-22
JP2015027308A (ja) 2015-02-12
EP2203556A1 (en) 2010-07-07
CA2704548A1 (en) 2009-05-07
WO2009058956A1 (en) 2009-05-07
JP2013121354A (ja) 2013-06-20
CO6210760A2 (es) 2010-10-20
AU2008318644B2 (en) 2013-05-02

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