JP5252746B2 - 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン - Google Patents

宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン Download PDF

Info

Publication number
JP5252746B2
JP5252746B2 JP2010532232A JP2010532232A JP5252746B2 JP 5252746 B2 JP5252746 B2 JP 5252746B2 JP 2010532232 A JP2010532232 A JP 2010532232A JP 2010532232 A JP2010532232 A JP 2010532232A JP 5252746 B2 JP5252746 B2 JP 5252746B2
Authority
JP
Japan
Prior art keywords
sequence
protein
laccase
nucleic acid
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2010532232A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011502483A5 (enExample
JP2011502483A (ja
Inventor
バオ、カイ
ワン、フアミン
Original Assignee
ダニスコ・ユーエス・インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク filed Critical ダニスコ・ユーエス・インク
Publication of JP2011502483A publication Critical patent/JP2011502483A/ja
Publication of JP2011502483A5 publication Critical patent/JP2011502483A5/ja
Application granted granted Critical
Publication of JP5252746B2 publication Critical patent/JP5252746B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2010532232A 2007-11-01 2008-10-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン Expired - Fee Related JP5252746B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US98443007P 2007-11-01 2007-11-01
US60/984,430 2007-11-01
PCT/US2008/081721 WO2009058956A1 (en) 2007-11-01 2008-10-30 Signal sequences and co-expressed chaperones for improving protein production in a host cell

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2013009289A Division JP2013121354A (ja) 2007-11-01 2013-01-22 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

Publications (3)

Publication Number Publication Date
JP2011502483A JP2011502483A (ja) 2011-01-27
JP2011502483A5 JP2011502483A5 (enExample) 2013-03-21
JP5252746B2 true JP5252746B2 (ja) 2013-07-31

Family

ID=40304979

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2010532232A Expired - Fee Related JP5252746B2 (ja) 2007-11-01 2008-10-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
JP2013009289A Pending JP2013121354A (ja) 2007-11-01 2013-01-22 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
JP2014200112A Withdrawn JP2015027308A (ja) 2007-11-01 2014-09-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2013009289A Pending JP2013121354A (ja) 2007-11-01 2013-01-22 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
JP2014200112A Withdrawn JP2015027308A (ja) 2007-11-01 2014-09-30 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン

Country Status (10)

Country Link
US (1) US20090221030A1 (enExample)
EP (1) EP2203556A1 (enExample)
JP (3) JP5252746B2 (enExample)
CN (1) CN101842479A (enExample)
AU (1) AU2008318644B2 (enExample)
BR (1) BRPI0818273A2 (enExample)
CA (1) CA2704548A1 (enExample)
CO (1) CO6210760A2 (enExample)
MX (1) MX2010004325A (enExample)
WO (1) WO2009058956A1 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0924180A8 (pt) 2008-12-24 2017-12-05 Danisco Us Inc Lacases e métodos de uso das mesmas em baixa temperatura
EP2403809B1 (en) 2009-03-03 2015-06-24 Danisco US Inc. Method of oxidative decolorization of dyes with enzymatically generated peracid
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
AR083471A1 (es) 2010-10-18 2013-02-27 Danisco Us Inc Modificacion local del color de telas teñidas empleando un sistema de lacasa
US20140123404A1 (en) 2011-04-06 2014-05-08 Danisco Us Inc. Laccase variants having increased expression and/or activity
JP2012235767A (ja) * 2011-04-27 2012-12-06 Toyota Motor Corp 変異トリコデルマ属微生物及びこれを用いたタンパク質の製造方法
RU2645252C2 (ru) 2012-01-05 2018-02-19 Глюкос Финланд Ой Клетка нитчатых грибов с дефицитом протеаз и способы ее применения
CN104066841B (zh) * 2012-01-23 2018-01-02 旭硝子株式会社 表达载体和蛋白质的制造方法
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
US9993534B2 (en) 2013-03-12 2018-06-12 Wisconsin Alumni Research Foundation Method of treating fungal infection
SG11201600115SA (en) 2013-07-10 2016-02-26 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
CN104694560A (zh) * 2013-12-04 2015-06-10 中国科学院天津工业生物技术研究所 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途
CN106661541B (zh) 2014-04-17 2021-09-03 贝林格尔·英格海姆Rcv两合公司 被工程化为过表达辅助蛋白的重组宿主细胞
AU2015248807B2 (en) 2014-04-17 2021-07-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2016025825A1 (en) * 2014-08-15 2016-02-18 Danisco Us Inc Compositions and methods for improved protein production
US10526609B2 (en) * 2016-08-01 2020-01-07 Aduro Biotech, Inc. Protein expression enhancer sequences and use thereof
JP7645076B2 (ja) * 2018-06-27 2025-03-13 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 転写因子の使用によってタンパク質発現を増加させるための手段及び方法
KR102138272B1 (ko) * 2018-09-19 2020-07-28 주식회사 바이오앱 BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법
KR102559048B1 (ko) * 2019-12-02 2023-07-26 주식회사 엘지화학 Cho 세포 유래 단백질 분비 인자 및 이를 포함하는 발현 벡터
CN112501141A (zh) * 2020-10-22 2021-03-16 重庆中元汇吉生物技术有限公司 一种增加人甲状腺过氧化物酶产量的试剂和表达方法
CN112175977B (zh) * 2020-10-30 2022-04-15 华中农业大学 一种米曲霉角蛋白酶基因及其表达载体与应用
CN112391402B (zh) * 2020-11-17 2023-03-28 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
CN112409464B (zh) * 2020-11-23 2022-04-22 江南大学 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用
WO2023039358A1 (en) * 2021-09-09 2023-03-16 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production
CN113736817B (zh) * 2021-10-08 2023-02-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
KR20240094203A (ko) * 2022-12-15 2024-06-25 씨제이제일제당 (주) 트리코더마 속 균주 유래 pdi를 이용하여 재조합 단백질을 생산하는 방법
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903294B (zh) * 2024-03-19 2024-06-14 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2322032T3 (es) * 1990-12-10 2009-06-16 Genencor Int Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei.
ATE195347T1 (de) * 1992-10-02 2000-08-15 Res Corp Technologies Inc Methoden der erhöhung der sekretion von überexpremierten proteinen
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US6008029A (en) * 1995-08-25 1999-12-28 Novo Nordisk Biotech Inc. Purified coprinus laccases and nucleic acids encoding the same
JP4307563B2 (ja) * 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
EP1032655B1 (en) * 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
CA2403842C (en) * 2000-03-24 2012-09-25 Genencor International, Inc. Increased production of secreted proteins by recombinant eukaryotic cells
US6358733B1 (en) * 2000-05-19 2002-03-19 Apolife, Inc. Expression of heterologous multi-domain proteins in yeast
JP2002065282A (ja) * 2000-09-04 2002-03-05 Iwate Prefecture シイタケラッカーゼ遺伝子
ATE482972T1 (de) * 2003-11-06 2010-10-15 Danisco Us Inc Tgf-beta1-bindende und geträgerte peptide
EP1689766B1 (en) * 2003-11-06 2012-04-25 Danisco US Inc. Expression in filamentous fungi of protease inhibitors and variants thereof
MXPA06013600A (es) * 2004-05-27 2007-03-15 Genencor Int Alfa amilasas acidoestables con actividad hidrolizante de almidon granular y composiciones enzimaticas.
EP2363460A3 (en) * 2004-12-30 2011-12-28 Genencor International, Inc. Acid fungal proteases
EP2092113A2 (en) * 2006-12-18 2009-08-26 Danisco US, INC., Genencor Division Laccase mediators and methods of use
EP2129685B1 (en) * 2007-03-21 2013-11-20 Danisco US Inc. Over expression of foldases and chaperones improves protein production

Also Published As

Publication number Publication date
MX2010004325A (es) 2010-05-20
CA2704548A1 (en) 2009-05-07
JP2013121354A (ja) 2013-06-20
US20090221030A1 (en) 2009-09-03
JP2015027308A (ja) 2015-02-12
EP2203556A1 (en) 2010-07-07
CO6210760A2 (es) 2010-10-20
CN101842479A (zh) 2010-09-22
AU2008318644A1 (en) 2009-05-07
BRPI0818273A2 (pt) 2014-10-14
WO2009058956A1 (en) 2009-05-07
AU2008318644B2 (en) 2013-05-02
JP2011502483A (ja) 2011-01-27

Similar Documents

Publication Publication Date Title
JP5252746B2 (ja) 宿主細胞におけるタンパク質生産を改善するためのシグナル配列及び共発現したシャペロン
DK2147104T3 (en) USING A ASPARAGINPROTEASE- (NSP24) SIGNAL SEQUENCE FOR heterologous protein EXPRESSION
CA2876287C (en) Agse-deficient strain
JP5087407B2 (ja) 酸性真菌プロテアーゼ
US8927250B2 (en) Polypeptides having alpha-amylase and granular starch hydrolyzing activity
KR102616331B1 (ko) 진균 균주 및 사용 방법
JP2010501181A (ja) 増加した生産力を伴うプルラナーゼ変異体
KR20140023313A (ko) 셀룰라제 조성물 및 리그노셀룰로스계 바이오매스의 발효성 당으로의 전환을 향상시키기 위해 상기 조성물을 사용하는 방법
US20110294191A1 (en) Increased production of aspartic proteases in filamentous fungal cells
US20200063166A1 (en) Zinc binuclear cluster transcriptional regulator-deficient strain
US8609386B2 (en) Polypeptides having tyrosinase activity and polynucleotides encoding same
DK2841589T3 (en) PROCEDURE FOR EXPRESSION OF POLYPEPTIDES
CN113316641B (zh) 串联蛋白表达
Yin et al. Construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in Aspergillus oryzae
AU2013205496A1 (en) Signal sequences and co-expressed chaperones for improving protein production in a host cell
CN120917131A (zh) 改善的基于黑麦的酒精饮料的生产
CN116583534A (zh) 前导肽和编码其的多核苷酸
Bulani Development of Yarrowia lipolytica expression systems for recombinant proteins
HK1158683A (en) Increased production of aspartic proteases in filamentous fungal cells
HK1178937A (en) Filamentous fungal host strains and dna constructs, and methods of use thereof
HK1135137B (en) Pullulanase variants with increased productivity

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120925

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121130

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20121207

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130122

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20130122

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130319

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130415

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20160426

Year of fee payment: 3

LAPS Cancellation because of no payment of annual fees