CN101842479A - 用于改良宿主细胞内蛋白质生产的信号序列和共表达的分子伴侣 - Google Patents

用于改良宿主细胞内蛋白质生产的信号序列和共表达的分子伴侣 Download PDF

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CN101842479A
CN101842479A CN200880114429A CN200880114429A CN101842479A CN 101842479 A CN101842479 A CN 101842479A CN 200880114429 A CN200880114429 A CN 200880114429A CN 200880114429 A CN200880114429 A CN 200880114429A CN 101842479 A CN101842479 A CN 101842479A
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sequence
protein
laccase
expression
nucleic acid
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鲍锴
王华明
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Danisco USA Inc
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Danisco USA Inc
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN200880114429A 2007-11-01 2008-10-30 用于改良宿主细胞内蛋白质生产的信号序列和共表达的分子伴侣 Pending CN101842479A (zh)

Applications Claiming Priority (3)

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US98443007P 2007-11-01 2007-11-01
US60/984,430 2007-11-01
PCT/US2008/081721 WO2009058956A1 (en) 2007-11-01 2008-10-30 Signal sequences and co-expressed chaperones for improving protein production in a host cell

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CN101842479A true CN101842479A (zh) 2010-09-22

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US (1) US20090221030A1 (enExample)
EP (1) EP2203556A1 (enExample)
JP (3) JP5252746B2 (enExample)
CN (1) CN101842479A (enExample)
AU (1) AU2008318644B2 (enExample)
BR (1) BRPI0818273A2 (enExample)
CA (1) CA2704548A1 (enExample)
CO (1) CO6210760A2 (enExample)
MX (1) MX2010004325A (enExample)
WO (1) WO2009058956A1 (enExample)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104694560A (zh) * 2013-12-04 2015-06-10 中国科学院天津工业生物技术研究所 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途
CN106795503A (zh) * 2014-08-15 2017-05-31 丹尼斯科美国公司 用于改善的蛋白质产生的组合物和方法
CN109890834A (zh) * 2016-08-01 2019-06-14 艾杜罗生物科技公司 蛋白质表达增强子序列及其用途
CN112391402A (zh) * 2020-11-17 2021-02-23 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
CN112501141A (zh) * 2020-10-22 2021-03-16 重庆中元汇吉生物技术有限公司 一种增加人甲状腺过氧化物酶产量的试剂和表达方法
CN112955547A (zh) * 2018-06-27 2021-06-11 贝林格尔·英格海姆Rcv两合公司 通过使用转录因子来增加蛋白质表达的手段和方法
CN113736817A (zh) * 2021-10-08 2021-12-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
CN114761418A (zh) * 2019-12-02 2022-07-15 株式会社Lg化学 Cho细胞衍生的蛋白分泌因子和包含它的表达载体
CN117903295A (zh) * 2024-03-19 2024-04-19 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903294A (zh) * 2024-03-19 2024-04-19 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2747813A1 (en) 2008-12-24 2010-07-01 Danisco Us Inc. Laccases and methods of use thereof at low temperature
CN102341354B (zh) 2009-03-03 2014-08-20 丹尼斯科美国公司 酶法产生的过酸对染料的氧化脱色-方法、组合物和套盒
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
CN103189564A (zh) 2010-10-18 2013-07-03 丹尼斯科美国公司 使用漆酶体系对染色织物进行局部颜色修改
EP2694647A1 (en) 2011-04-06 2014-02-12 Danisco US Inc. Laccase variants having increased expression and/or activity
JP2012235767A (ja) * 2011-04-27 2012-12-06 Toyota Motor Corp 変異トリコデルマ属微生物及びこれを用いたタンパク質の製造方法
US9567596B2 (en) 2012-01-05 2017-02-14 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
WO2013111754A1 (ja) * 2012-01-23 2013-08-01 旭硝子株式会社 発現ベクターおよび蛋白質の製造方法
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
US9993534B2 (en) * 2013-03-12 2018-06-12 Wisconsin Alumni Research Foundation Method of treating fungal infection
MX2016000306A (es) 2013-07-10 2016-08-08 Novartis Ag Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas.
WO2015158808A2 (en) 2014-04-17 2015-10-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
SG11201608080RA (en) 2014-04-17 2016-10-28 Boehringer Ingelheim Rcv Gmbh Recombinant host cell for expressing proteins of interest
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
KR102138272B1 (ko) * 2018-09-19 2020-07-28 주식회사 바이오앱 BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법
CN112175977B (zh) * 2020-10-30 2022-04-15 华中农业大学 一种米曲霉角蛋白酶基因及其表达载体与应用
CN112409464B (zh) * 2020-11-23 2022-04-22 江南大学 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用
US20240376512A1 (en) * 2021-09-09 2024-11-14 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773245A (en) * 1992-10-02 1998-06-30 Research Corporation Technologies, Inc. Methods for increasing secretion of overexpressed proteins

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1225227T3 (da) * 1990-12-10 2009-06-22 Genencor Int Forbedret forsukring af cellulose ved kloning og amplifikation af beta-glucosidase genet af trichoderma
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US6008029A (en) * 1995-08-25 1999-12-28 Novo Nordisk Biotech Inc. Purified coprinus laccases and nucleic acids encoding the same
EP0973917B1 (en) * 1997-04-07 2004-03-03 Unilever N.V. Agrobacterium mediated transformation of moulds, in particular those belonging to the genus aspergillus
KR100762164B1 (ko) * 1997-11-21 2007-10-01 노보자임스 에이/에스 프로테아제 변이체 및 조성물
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
ATE394496T1 (de) * 2000-03-24 2008-05-15 Genencor Int Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen
US6358733B1 (en) * 2000-05-19 2002-03-19 Apolife, Inc. Expression of heterologous multi-domain proteins in yeast
JP2002065282A (ja) * 2000-09-04 2002-03-05 Iwate Prefecture シイタケラッカーゼ遺伝子
CA2545053C (en) * 2003-11-06 2016-04-19 Genencor International, Inc. Expression in filamentous fungi of protease inhibitors and variants thereof
US20080113917A1 (en) * 2003-11-06 2008-05-15 Day Anthony G Tgfbeta Supported and Binding Peptides
MXPA06013600A (es) * 2004-05-27 2007-03-15 Genencor Int Alfa amilasas acidoestables con actividad hidrolizante de almidon granular y composiciones enzimaticas.
US7429476B2 (en) * 2004-12-30 2008-09-30 Genencor International, Inc. Acid fungal proteases
MX2009005734A (es) * 2006-12-18 2009-06-10 Danisco Us Inc Genencor Div Mediadores de lacasa y metodos de uso.
US8546126B2 (en) * 2007-03-21 2013-10-01 Danisco Us Inc. Over expression of foldases and chaperones improves protein production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5773245A (en) * 1992-10-02 1998-06-30 Research Corporation Technologies, Inc. Methods for increasing secretion of overexpressed proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JARI J RAUTIO ET AL.: "Physiological evaluation of the filamentous fungus Trichoderma Physiological evaluation of the filamentous fungus Trichoderma", 《BMC BIOTECHNOLOGY》 *
LAURA-LEENA KIISKINEN ET AL.: "Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme", 《MICROBIOLOGY》 *
P. J. PUNT ET AL.: "Analysis of the role of the gene bipA, encoding the major endoplasmic reticulum chaperone protein in the secretion of homologous and heterologous proteins in black Aspergilli", 《APPL MICROBIOL BIOTECHNOL》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104694560A (zh) * 2013-12-04 2015-06-10 中国科学院天津工业生物技术研究所 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途
CN106795503A (zh) * 2014-08-15 2017-05-31 丹尼斯科美国公司 用于改善的蛋白质产生的组合物和方法
CN109890834A (zh) * 2016-08-01 2019-06-14 艾杜罗生物科技公司 蛋白质表达增强子序列及其用途
CN112955547A (zh) * 2018-06-27 2021-06-11 贝林格尔·英格海姆Rcv两合公司 通过使用转录因子来增加蛋白质表达的手段和方法
CN114761418A (zh) * 2019-12-02 2022-07-15 株式会社Lg化学 Cho细胞衍生的蛋白分泌因子和包含它的表达载体
CN112501141A (zh) * 2020-10-22 2021-03-16 重庆中元汇吉生物技术有限公司 一种增加人甲状腺过氧化物酶产量的试剂和表达方法
CN112391402A (zh) * 2020-11-17 2021-02-23 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
CN113736817A (zh) * 2021-10-08 2021-12-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
CN117903295A (zh) * 2024-03-19 2024-04-19 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903294A (zh) * 2024-03-19 2024-04-19 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903294B (zh) * 2024-03-19 2024-06-14 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

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CO6210760A2 (es) 2010-10-20
MX2010004325A (es) 2010-05-20
BRPI0818273A2 (pt) 2014-10-14
JP2011502483A (ja) 2011-01-27
JP5252746B2 (ja) 2013-07-31
JP2013121354A (ja) 2013-06-20
CA2704548A1 (en) 2009-05-07
JP2015027308A (ja) 2015-02-12
US20090221030A1 (en) 2009-09-03
WO2009058956A1 (en) 2009-05-07
AU2008318644B2 (en) 2013-05-02
EP2203556A1 (en) 2010-07-07
AU2008318644A1 (en) 2009-05-07

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