CO2023013173A2 - Aumento de la transformabilidad e inducción de haploidía en plantas - Google Patents

Aumento de la transformabilidad e inducción de haploidía en plantas

Info

Publication number
CO2023013173A2
CO2023013173A2 CONC2023/0013173A CO2023013173A CO2023013173A2 CO 2023013173 A2 CO2023013173 A2 CO 2023013173A2 CO 2023013173 A CO2023013173 A CO 2023013173A CO 2023013173 A2 CO2023013173 A2 CO 2023013173A2
Authority
CO
Colombia
Prior art keywords
plants
plant
haploidy
induction
methods
Prior art date
Application number
CONC2023/0013173A
Other languages
English (en)
Inventor
Timothy Joseph Kelliher
Brent Delzer
David Stewart Skibbe
Jason Nichols
Original Assignee
Syngenta Crop Protection Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection Ag filed Critical Syngenta Crop Protection Ag
Publication of CO2023013173A2 publication Critical patent/CO2023013173A2/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01004—Phospholipase A2 (3.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

RESUMEN DE LA INVENCIÓN En el presente documento se proporcionan plantas de maíz sumamente transformables, denominadas plantas HI-NA, y métodos para su producción y uso. Una planta HI-NA, como se desvela en el presente documento, es homocigota para un alelo mutante de pérdida de función en el gen de la fosfolipasa A2α de tipo patatina (MATL) y al menos heterocigota para uno o más alelos de los QTL y/o de los genes que son responsables del aumento de la inducción de haploidía y/o la frecuencia de transformación en las plantas. Una planta HI-NA, como se desvela en el presente documento, también puede tener un acervo de citotipo A, que puede hacerla sumamente transformable. También se proporcionan métodos para producir plantas HI-NA y métodos para usar una planta HI-NA para editar ADN genómico vegetal.
CONC2023/0013173A 2021-04-01 2023-10-02 Aumento de la transformabilidad e inducción de haploidía en plantas CO2023013173A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163169316P 2021-04-01 2021-04-01
PCT/US2022/022271 WO2022212318A1 (en) 2021-04-01 2022-03-29 Increased transformability and haploid induction in plants

Publications (1)

Publication Number Publication Date
CO2023013173A2 true CO2023013173A2 (es) 2023-10-30

Family

ID=83456694

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2023/0013173A CO2023013173A2 (es) 2021-04-01 2023-10-02 Aumento de la transformabilidad e inducción de haploidía en plantas

Country Status (13)

Country Link
EP (1) EP4319545A1 (es)
JP (1) JP2024512692A (es)
KR (1) KR20230163460A (es)
CN (1) CN117202778A (es)
AR (1) AR125249A1 (es)
AU (1) AU2022249024A1 (es)
BR (1) BR112023019929A2 (es)
CA (1) CA3212460A1 (es)
CL (1) CL2023002885A1 (es)
CO (1) CO2023013173A2 (es)
CR (1) CR20230463A (es)
IL (1) IL306026A (es)
WO (1) WO2022212318A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023225469A2 (en) * 2022-05-19 2023-11-23 Syngenta Crop Protection Ag Conferring cytoplasmic male sterility
EP4360451A1 (en) * 2022-10-25 2024-05-01 Limagrain Europe Mutants for haploid induction

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10448588B2 (en) * 2013-03-15 2019-10-22 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
CN111763687B (zh) * 2019-03-12 2021-12-07 中国农业大学 一种基于基因编辑技术快速培育玉米单倍体诱导系的方法
WO2020205334A1 (en) * 2019-04-01 2020-10-08 Syngenta Crop Protection Ag Increasing plant transformability by cytotype transfer

Also Published As

Publication number Publication date
CA3212460A1 (en) 2022-10-06
AR125249A1 (es) 2023-06-28
CN117202778A (zh) 2023-12-08
AU2022249024A1 (en) 2023-10-05
AU2022249024A9 (en) 2024-02-22
IL306026A (en) 2023-11-01
CR20230463A (es) 2023-12-12
WO2022212318A1 (en) 2022-10-06
BR112023019929A2 (pt) 2024-01-16
KR20230163460A (ko) 2023-11-30
EP4319545A1 (en) 2024-02-14
JP2024512692A (ja) 2024-03-19
CL2023002885A1 (es) 2024-03-08

Similar Documents

Publication Publication Date Title
CO2023013173A2 (es) Aumento de la transformabilidad e inducción de haploidía en plantas
BR112018011242A2 (pt) métodos para modificação genética de plantas
Huang et al. CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in the global pest, diamondback moth (Plutella xylostella)
AR110581A1 (es) Modificación genética de cultivos a base de edición genómica y producción de plantas braquíticas
CO2019005085A2 (es) Composiciones y métodos para alterar la floración y arquitectura de planta para mejorar el potencial de rendimiento
AR100216A1 (es) Edición de genes multiplexados
RU2672335C2 (ru) Гены, обеспечивающие устойчивость подсолнечника к ложной мучнистой росе
CL2021002285A1 (es) Métodos para la producción vegetal clonal.
AR104024A1 (es) Métodos y composiciones para la introgresión acelerada de rasgos
UY31585A1 (es) Plantas madereras con las caracteristicas de desarrollo mejoradas y método para su obtencion usando factores de transcripcion
WO2007130804A3 (en) Method for producing fertile crosses between wild and domestic soybean species
ES2876403T3 (es) Plantas resistentes a Phytophthora pertenecientes a la familia Solanaceae
BR112018068868A2 (pt) plantas transgênicas com traços aprimorados
AR118352A1 (es) Constructo polinucleotídico para mejorar características agronómicas en plantas de cultivo
WO2015155607A3 (en) Compositions and methods for enhancing plant breeding
MX2022001130A (es) Loci geneticos asociados con resistencia a enfermedades en soja.
JP2018523988A5 (es)
BR112021021149A2 (pt) Métodos e composições para a geração de alelos dominantes utilizando a edição de genomas
AR112465A1 (es) Métodos y composiciones para la edición de genes de base viral en plantas
AR072157A1 (es) Metodos y medios para aumentar la eficacia en el uso de agua en plantas
AR068542A1 (es) Plantas que tienen biomasa aumentada
AR120136A1 (es) Plantas modificadas que comprende una mutación en una secuencia de ácido nucleico lazy4
MA43463B1 (fr) Quantite et rendement accrus de sucres solubles attribues aux fruits dans des plans de tomate
CO2019003924A2 (es) Plantas de crisantemo resistentes a la roya blanca
AR094339A1 (es) Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de la familia de la lacasa