CR20230463A - Aumento de la transformabilidad e inducción de haploidía en plantas - Google Patents

Aumento de la transformabilidad e inducción de haploidía en plantas

Info

Publication number
CR20230463A
CR20230463A CR20230463A CR20230463A CR20230463A CR 20230463 A CR20230463 A CR 20230463A CR 20230463 A CR20230463 A CR 20230463A CR 20230463 A CR20230463 A CR 20230463A CR 20230463 A CR20230463 A CR 20230463A
Authority
CR
Costa Rica
Prior art keywords
plants
plant
methods
haploid induction
disclosed
Prior art date
Application number
CR20230463A
Other languages
English (en)
Inventor
David Stewart Skibbe
Brent Delzer
Jason Nichols
Timothy Joseph Kelliher
Original Assignee
Syngenta Crop Protection Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection Ag filed Critical Syngenta Crop Protection Ag
Publication of CR20230463A publication Critical patent/CR20230463A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01004—Phospholipase A2 (3.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

En el presente documento se proporcionan plantas de maíz sumamente transformables, denominadas plantas HI-NA, y métodos para su producción y uso. Una planta HI-NA, como se desvela en el presente documento, es homocigota para un alelo mutante de pérdida de función en el gen de la fosfolipasa A2a de tipo patatina (MATL) y al menos heterocigota para uno o más alelos de los QTL y/o de los genes que son responsables del aumento de la inducción de haploidía y/o la frecuencia de transformación en las plantas. Una planta HI-NA, como se desvela en el presente documento, también puede tener un acervo de citotipo A, que puede hacerla sumamente transformable. También se proporcionan métodos para producir plantas HI-NA y métodos para usar una planta HI-NA para editar ADN genómico vegetal.
CR20230463A 2021-04-01 2022-03-29 Aumento de la transformabilidad e inducción de haploidía en plantas CR20230463A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163169316P 2021-04-01 2021-04-01
PCT/US2022/022271 WO2022212318A1 (en) 2021-04-01 2022-03-29 Increased transformability and haploid induction in plants

Publications (1)

Publication Number Publication Date
CR20230463A true CR20230463A (es) 2023-12-12

Family

ID=83456694

Family Applications (1)

Application Number Title Priority Date Filing Date
CR20230463A CR20230463A (es) 2021-04-01 2022-03-29 Aumento de la transformabilidad e inducción de haploidía en plantas

Country Status (15)

Country Link
EP (1) EP4319545A1 (es)
JP (1) JP2024512692A (es)
KR (1) KR20230163460A (es)
CN (1) CN117202778A (es)
AR (1) AR125249A1 (es)
AU (1) AU2022249024A1 (es)
BR (1) BR112023019929A2 (es)
CA (1) CA3212460A1 (es)
CL (1) CL2023002885A1 (es)
CO (1) CO2023013173A2 (es)
CR (1) CR20230463A (es)
IL (1) IL306026A (es)
MX (1) MX2023011473A (es)
WO (1) WO2022212318A1 (es)
ZA (1) ZA202309197B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023225469A2 (en) * 2022-05-19 2023-11-23 Syngenta Crop Protection Ag Conferring cytoplasmic male sterility
EP4360451A1 (en) * 2022-10-25 2024-05-01 Limagrain Europe Mutants for haploid induction

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10448588B2 (en) * 2013-03-15 2019-10-22 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
CN111763687B (zh) * 2019-03-12 2021-12-07 中国农业大学 一种基于基因编辑技术快速培育玉米单倍体诱导系的方法
AU2020254415A1 (en) * 2019-04-01 2021-10-07 Syngenta Crop Protection Ag Increasing plant transformability by cytotype transfer

Also Published As

Publication number Publication date
EP4319545A1 (en) 2024-02-14
AU2022249024A1 (en) 2023-10-05
CL2023002885A1 (es) 2024-03-08
ZA202309197B (en) 2024-06-26
CO2023013173A2 (es) 2023-10-30
CN117202778A (zh) 2023-12-08
BR112023019929A2 (pt) 2024-01-16
IL306026A (en) 2023-11-01
AR125249A1 (es) 2023-06-28
JP2024512692A (ja) 2024-03-19
MX2023011473A (es) 2023-10-19
CA3212460A1 (en) 2022-10-06
KR20230163460A (ko) 2023-11-30
WO2022212318A1 (en) 2022-10-06
AU2022249024A9 (en) 2024-02-22

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