BR112023019929A2 - Métodos para identificar ou produzir uma planta de milho indutora haploide transformável, para transformar ou obter uma planta de milho transformada e para edição de dna genômico de plantas, genoma de planta de milho modificado, e uso de uma célula de planta de milho modificada, tecido de planta, semente, parte de planta ou planta compreendendo uma mutação homozigótica de perda de função no gene da fosfolipase a2-alfa semelhante à patatina (matl) e pelo menos heterozigótica para um alelo hi em pelo menos um locus de traço quantitativo (qtl) associado ao aumento da indução haplóide (hi-qtl) - Google Patents
Métodos para identificar ou produzir uma planta de milho indutora haploide transformável, para transformar ou obter uma planta de milho transformada e para edição de dna genômico de plantas, genoma de planta de milho modificado, e uso de uma célula de planta de milho modificada, tecido de planta, semente, parte de planta ou planta compreendendo uma mutação homozigótica de perda de função no gene da fosfolipase a2-alfa semelhante à patatina (matl) e pelo menos heterozigótica para um alelo hi em pelo menos um locus de traço quantitativo (qtl) associado ao aumento da indução haplóide (hi-qtl)Info
- Publication number
- BR112023019929A2 BR112023019929A2 BR112023019929A BR112023019929A BR112023019929A2 BR 112023019929 A2 BR112023019929 A2 BR 112023019929A2 BR 112023019929 A BR112023019929 A BR 112023019929A BR 112023019929 A BR112023019929 A BR 112023019929A BR 112023019929 A2 BR112023019929 A2 BR 112023019929A2
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- corn plant
- qtl
- methods
- transformable
- Prior art date
Links
- 241000196324 Embryophyta Species 0.000 title abstract 13
- 241001057636 Dracaena deremensis Species 0.000 title abstract 9
- 108700028369 Alleles Proteins 0.000 title abstract 4
- 230000006698 induction Effects 0.000 title abstract 3
- 230000001939 inductive effect Effects 0.000 title abstract 2
- 230000004777 loss-of-function mutation Effects 0.000 title abstract 2
- 230000001131 transforming effect Effects 0.000 title abstract 2
- 108010064785 Phospholipases Proteins 0.000 title 1
- 101710097260 cGAMP-activated phospholipase Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000003658 Group IV Phospholipases A2 Human genes 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01004—Phospholipase A2 (3.1.1.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Physiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
métodos para identificar ou produzir uma planta de milho indutora haploide transformável, para transformar ou obter uma planta de milho transformada e para edição de dna genômico de plantas, genoma de planta de milho modificado, e uso de uma célula de planta de milho modificada, tecido de planta, semente, parte de planta ou planta compreendendo uma mutação homozigótica de perda de função no gene da fosfolipase a2-alfa semelhante à patatina (matl) e pelo menos heterozigótica para um alelo hi em pelo menos um locus de traço quantitativo (qtl) associado ao aumento da indução haplóide (hi-qtl). são fornecidas aqui plantas de milho altamente transformáveis, referidas como plantas hi-na, e métodos de sua produção e uso. uma planta hi-na, como aqui descrito, é homozigótica para um alelo mutante com perda de função no gene da fosfolipase a2a semelhante à patatina (matl) e pelo menos heterozigótica para um ou mais qtl e/ou alelos genéticos que são responsáveis por aumento da indução haploide e/ou frequência de transformação em plantas. uma planta hi-na, tal como aqui descrita, também pode ter um fundo de citótipo a, o que pode torná-la altamente transformável. também são fornecidos métodos de produção de plantas hi-na e métodos de utilização de uma planta hi-na para edição de dna genômico de plantas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163169316P | 2021-04-01 | 2021-04-01 | |
| PCT/US2022/022271 WO2022212318A1 (en) | 2021-04-01 | 2022-03-29 | Increased transformability and haploid induction in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023019929A2 true BR112023019929A2 (pt) | 2024-01-16 |
Family
ID=83456694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023019929A BR112023019929A2 (pt) | 2021-04-01 | 2022-03-29 | Métodos para identificar ou produzir uma planta de milho indutora haploide transformável, para transformar ou obter uma planta de milho transformada e para edição de dna genômico de plantas, genoma de planta de milho modificado, e uso de uma célula de planta de milho modificada, tecido de planta, semente, parte de planta ou planta compreendendo uma mutação homozigótica de perda de função no gene da fosfolipase a2-alfa semelhante à patatina (matl) e pelo menos heterozigótica para um alelo hi em pelo menos um locus de traço quantitativo (qtl) associado ao aumento da indução haplóide (hi-qtl) |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP4319545A1 (pt) |
| JP (1) | JP2024512692A (pt) |
| KR (1) | KR20230163460A (pt) |
| CN (1) | CN117202778A (pt) |
| AR (1) | AR125249A1 (pt) |
| AU (1) | AU2022249024A1 (pt) |
| BR (1) | BR112023019929A2 (pt) |
| CA (1) | CA3212460A1 (pt) |
| CL (1) | CL2023002885A1 (pt) |
| CO (1) | CO2023013173A2 (pt) |
| CR (1) | CR20230463A (pt) |
| IL (1) | IL306026A (pt) |
| MX (1) | MX2023011473A (pt) |
| WO (1) | WO2022212318A1 (pt) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023225469A2 (en) * | 2022-05-19 | 2023-11-23 | Syngenta Crop Protection Ag | Conferring cytoplasmic male sterility |
| EP4360451A1 (en) * | 2022-10-25 | 2024-05-01 | Limagrain Europe | Mutants for haploid induction |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10448588B2 (en) * | 2013-03-15 | 2019-10-22 | Syngenta Participations Ag | Haploid induction compositions and methods for use therefor |
| CN111763687B (zh) * | 2019-03-12 | 2021-12-07 | 中国农业大学 | 一种基于基因编辑技术快速培育玉米单倍体诱导系的方法 |
| CR20210496A (es) * | 2019-04-01 | 2021-10-25 | Syngenta Crop Protection Ag | Aumento de la transformabilidad de las plantas por transferencia de citotipo |
-
2022
- 2022-03-29 CA CA3212460A patent/CA3212460A1/en active Pending
- 2022-03-29 WO PCT/US2022/022271 patent/WO2022212318A1/en active Application Filing
- 2022-03-29 BR BR112023019929A patent/BR112023019929A2/pt unknown
- 2022-03-29 AU AU2022249024A patent/AU2022249024A1/en active Pending
- 2022-03-29 KR KR1020237036525A patent/KR20230163460A/ko unknown
- 2022-03-29 CR CR20230463A patent/CR20230463A/es unknown
- 2022-03-29 CN CN202280026745.8A patent/CN117202778A/zh active Pending
- 2022-03-29 MX MX2023011473A patent/MX2023011473A/es unknown
- 2022-03-29 EP EP22781993.5A patent/EP4319545A1/en active Pending
- 2022-03-29 JP JP2023560459A patent/JP2024512692A/ja active Pending
- 2022-03-29 IL IL306026A patent/IL306026A/en unknown
- 2022-03-30 AR ARP220100770A patent/AR125249A1/es unknown
-
2023
- 2023-09-27 CL CL2023002885A patent/CL2023002885A1/es unknown
- 2023-10-02 CO CONC2023/0013173A patent/CO2023013173A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA3212460A1 (en) | 2022-10-06 |
| JP2024512692A (ja) | 2024-03-19 |
| IL306026A (en) | 2023-11-01 |
| WO2022212318A1 (en) | 2022-10-06 |
| CN117202778A (zh) | 2023-12-08 |
| AU2022249024A1 (en) | 2023-10-05 |
| KR20230163460A (ko) | 2023-11-30 |
| CO2023013173A2 (es) | 2023-10-30 |
| AR125249A1 (es) | 2023-06-28 |
| CL2023002885A1 (es) | 2024-03-08 |
| CR20230463A (es) | 2023-12-12 |
| EP4319545A1 (en) | 2024-02-14 |
| AU2022249024A9 (en) | 2024-02-22 |
| MX2023011473A (es) | 2023-10-19 |
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