CN1918970A - Seeds production method for pumpkin rootstock - Google Patents
Seeds production method for pumpkin rootstock Download PDFInfo
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- CN1918970A CN1918970A CN 200610068737 CN200610068737A CN1918970A CN 1918970 A CN1918970 A CN 1918970A CN 200610068737 CN200610068737 CN 200610068737 CN 200610068737 A CN200610068737 A CN 200610068737A CN 1918970 A CN1918970 A CN 1918970A
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Abstract
The invention discloses a sowing method of pumpkin stock, which comprises the following steps: adopting Chinese round pumpkin in the Shandong province to cross for three generations, separating to purify, identifying to sieve self-crossing YN-3 to grow stably under low-temperature and weak light condition as mother, selecting self-crossing CN-3 of Indian long pumpkin to resist low-temperature, drought and disease as father, crossing to obtain the first-filial generation.
Description
Technical field
The present invention relates to a kind of producing method for seed of pumpkin rootstock.
Background technology
Along with science and technology development, in the melon and fruit planting technology of modern agriculture, often adopt stock, realize defending and fighting against diseases by grafting and root-changing.At present, the used stock of plantation melon and fruit, be divided into three kinds substantially: 1, make stock with black pumpkin, it can only be as the stock of cucumber, and range of application is narrow; 2, make stock with cucurbit, as China Patent No. is that 97104470.8 disclosed " breeding methods of first generation watermelon-stock hybrid " and China Patent No. are the cucurbit stock that 200310108868.5 disclosed " selections of the special-purpose hybridization of facility watermelon blight control stock " are cultivated, though this cucurbit stock can be as the stock of watermelon, cucumber, the effect of anti-blight and anti-continuous cropping is still poor; 3, make stock with the hybridization pumpkin, though this hybridization pumpkin rootstock can be as the stock of cucumber, watermelon and muskmelon, affinity is poor.
Summary of the invention
The producing method for seed that the purpose of this invention is to provide the pumpkin rootstock that a kind of resistance against diseases is strong, affinity is good.
In order to realize above-mentioned purpose, the producing method for seed of the pumpkin rootstock that is provided, with select to be grown in China's circle pumpkin in Shandong, through the selfing separation and purification of at least 3 generations, evaluation and screening goes out the inbred line YN-3 that plant strain growth is sane under the low temperature and poor light condition, lower temperature resistance is strong and is female parent, with select to be grown in the long pumpkin of India in Shandong, through the selfing separation and purification of at least 3 generations, that evaluation and screening goes out is low temperature resistant, drought resistance and the strong inbred line CN-3 of disease resistance are male parent, hybridizes first generation cross-fertilize seed.This first generation cross-fertilize seed YN-3 * CN-3 names and is the pumpkin anvil.
With the producing method for seed of pumpkin rootstock provided by the present invention cultivate the pumpkin anvil that crossbreed grew, have following characteristics:
1, resistance against diseases is strong, and to powdery mildew, fusarium wilt, climing rot and mosaic virus, to compare resistance against diseases as stock stronger with cucurbit;
2, output height, with pumpkin anvil seedling and watermelon, cucumber or the grafting of muskmelon seedling, its output is compared with own-rooted tree and is improved 20%-30%, and bears fruit in advance;
3, the grafting affinity with pumpkin anvil seedling and watermelon, cucumber or muskmelon seedling is good, symbiosis affinity is strong;
4, soil property is required not strict, can continuous cropping, strong stress resistance;
5, production of hybrid seeds program is easy, is easy to promote.
Embodiment
Be elaborated below in conjunction with the producing method for seed of specific embodiment to pumpkin rootstock provided by the present invention.
1, maternal seed selection: the China's circle pumpkin that is grown in Shandong, its proterties is that root system is very flourishing, main root buries and reaches 2 meters deeply, lateral root has three grades to level Four, surplus the one-level lateral root has 20, be about 50cm, reach most 140cm, lateral root to around stretch and to reach 80cm-170cm, main Root Distribution is in the dark native top layer of 0-30cm, it is heart-shaped that leaf is, green no spot, female flower is given birth at the axil place, male flower, side shoot and tendril, four leaf phases were plucked core, stayed son climing, and the female flower that climing the 4th to the 5th joint of son occurs will be extractd because of trophosome is little, thereafter respectively at the 8th to the 9th joint, the the 13 to 14 joint, the 17 to the 18 joint is all met with fruit, and fruit is light green colour band white dot.Through the selfing separation and purification of 3 generations, natural evaluation and screening goes out the inbred line YN-3 that plant strain growth is sane under the low temperature and poor light condition, lower temperature resistance is strong and is female parent to above-mentioned China's circle pumpkin.
2, the seed selection of male parent: the long pumpkin of India that is grown in Shandong, its proterties is short life, plant is uprightly clustered, the leaf flowering shrubs gives birth to, and 3-4 short stems is arranged, a little less than the growth potential, well developed root system, the leaf color is a bottle green, the elongated and hollow of petiole, no stipule, the blade face is coarse pubescence, vein has hickie, and female flower, male flower, side shoot and tendril are given birth to by the axil place, and fruit is cylindrical, the melon skin color is orange bloom, there is obvious rib ditch on the surface, and it is filbert that seed is, and has the characteristic of high mildew-resistance, fusarium wilt and climing withered disease.Through the selfing separation and purification of 3 generations, that natural evaluation and screening goes out is low temperature resistant, drought resistance and the strong inbred line CN-3 of disease resistance are male parent to the long pumpkin of above-mentioned India.
3, hybrid seeding:
1), this configuration of father and mother: this disposed father and mother by 1: 10.
The sowing in 15 days-20 days in advance of A, male parent, so that more male flower is provided, the female bud on the plant is extractd in two climing trainings, the not nitrogen fertilizer application that applies fertilizer preferably so that help the generation and the growth of male flower, improves the quality of male pollen.
B, female parent are provided with the isolated area plantation, and seed farm and other pumpkin are wanted at interval more than 2000 meters, to guarantee the purity of seed.Select that the soil is porous, well-drained field piece, fertilizing amount can not be excessive, applied nitrogen not, executes phosphate fertilizer after bearing fruit, so that it is full that melon seeds is enriched.The four leaf phases of plant are plucked core, stay the climing parallel growth of two strips, the female flower that climing the 4th to the 5th joint of every strip occurs will be extractd because of trophosome is little, thereafter at the 8th to the 9th joint, the 13 to 14 joint, the 17 to the 18 joint 6 female flowers appear respectively, treat to use when female flower is open the male pollen hybridization pollination of male parent, 5-6 of the climing knot of a strip planted melon.This training stays the fruit method suitable because of the climing growing way of two strips, bloom, pollination, as a result the time concentrated, the seed production height.
2), this seedling management of father and mother:
A, sowing: sow during local temperature rise to 14 in spring ℃-20 ℃, open the cave before the sowing earlier, water vola water, 3-4 seeds of every bunch planting, water oozes the thick fine earth of bonnet 2cm, can emerge in 7 days-8 days, the 2 strain healthy seedlings of selecting and remain during thinning are pulled out weak seedling and lopsided seedling, generally do not water seedling stage, if lack of water and fertilizer deficiency are opened shallow ridges and are executed a small amount of composite fertilizer and water, subsequently earthing around the cave.
B, field planting: the plant growing way is strong, and every strain is stayed two climing, 450 of every mu of plantations, single climing 900 strains, and preferably line-spacing 2.5m, spacing in the rows 0.5m-0.6m in time water after the field planting and look into seedling at any time, will in time fill the gaps with seedlings in case be short of seedling, and guarantee the neat seedling of full stand.
C, intertill and clean tillage: will carry out repeatedly intertill and clean tillage before the climing envelope ridge from the field planting to the melon, the purpose of intertillage is a chesson, increases the gas permeability of soil, improves ground temperature, keeps soil moisture, helps the growth of root system.
D, fertilizing, watering: the management of liquid manure will be grasped with different breeding times flexibly according to the growing way of soil fertility, melon seedling.To stretching the climing phase,, execute 1 time-2 times loose stool water in conjunction with watering from field planting, promote tiller, if fertile soil then only waters and do not apply fertilizer if a little less than the seedling.Stretch climing early stage, if the good conditions for soil moisture should water or not water less, promote root of hair, be beneficial to strong seedling.Bloom bear fruit before, prevent the cauline leaf over growth, in order to avoid influence is bloomed and beared fruit.Bear fruit plant and to execute nitrogen-phosphorus-potassium compound fertilizer to envelope before the ridge, execute 15kg-20kg for every mu, water after the fertilising, current fertilising plays an important role to promoting fruit to expand.
3), hybridization pollination:
A, for maternal plant, extract all male flower flower buds and side shoot, tendril in conjunction with training, prevent and treat ground insects such as worm ant simultaneously, prevent pollination hybridization.
B, to the female flower on the maternal plant that will open morning next day, pressed from both sides flower, bundle flower or bagging at dusk on the same day, bagging is made with red glass paper, specification 12.5cm * 9.4cm, sack is folding, fixes with clip.
C, be to pluck the male flower flower bud at dusk open the previous day the male flower flower bud to the collection of male flower, tie petal with rubber band, put plastic sack again, put into bucket, it is standby to carry out health.
The Best Times of D, hybridization pollination is 5 o'clock-8 o'clock morning, this moment, female flower was just open, at first standby male flower flower bud is removed petal during pollination, remove the female flower bagging again, buttress female flower on the other hand, another hand is evenly coated in the pollen on the male flower core around the female chapiter, and 1 male flower flower bud can be awarded 3-5 female flowers.Arrangement petal in pollination back puts the red glass paper bag again, seals sack, colored belt on the bundle.
4), plant the strain management:
After first female flower is beared fruit, treat that kind of strain regeneration grows coring behind 3-5 leaves, later on according to the growing way of rattan leaf, application in time and strengthen the extermination of disease and insect pest.
5), gather and get seed:
Pollinating from female flower, kind of melon is ripe to need 50 days, the fully ripe kind melon of gathering, and after-ripening is cutd open melon and is got kind after 15 days-20 days, cleans, the cool solarization.
6), selected:
Seed after the cold solarization is removed brokenly seed, foreign material, and the seed collecting amount reaches 20kg-30kg for every mu.
Test shows, the seed that the producing method for seed of pumpkin rootstock provided by the present invention makes, its rice shoot is as pumpkin rootstock and watermelon, cucumber or the grafting method grafting routinely of muskmelon rice shoot, affinity is good, symbiosis affinity is strong, high resistance to wilt good, and improve output, do not change quality, see following subordinate list in detail.
The different graft stock watermelon blight of the table 1 incidence table of comparisons
| Time | The stock kind | The strain number | The withered strain number of dying of illness | Dead plant rate (%) |
| 2004 | The cucurbit anvil | 100 | 13 | 13 |
| Own-rooted tree | 100 | 47 | 47 | |
| The pumpkin anvil | 100 | 0 | 0 | |
| 2005 | The cucurbit anvil | 100 | 17 | 17 |
| Own-rooted tree | 100 | 53 | 53 | |
| The pumpkin anvil | 100 | 0 | 0 |
The main proterties contrast table of the different graft stock watermelons of table 2
| The stock kind | The transplanting phase (moon/day) | Plant the climing number of blade of back 30 God | Sit the melon time (moon/day) | Beginning adopts the time (moon/day) | Single melon heavy (kg) | The center pol | The edge pol | Taste |
| The cucurbit anvil | 2/11 | 20 | 3/19 | 4/23 | 6.1 | 11.6 | 8.6 | Free from extraneous odour |
| Own-rooted tree | 2/11 | 14.5 | 3/28 | 5/1 | 3.8 | 11.8 | 8.8 | Free from extraneous odour |
| The pumpkin anvil | 2/11 | 26 | 3/16 | 4/22 | 6.6 | 11.8 | 8.8 | Free from extraneous odour |
The different graft stock cucumber fusarium axysporum of the table 3 incidence table of comparisons
| Time | The stock kind | The strain number | The withered strain number of dying of illness | Dead plant rate (%) |
| 2004 | Own-rooted tree | 60 | 28 | 46.7 |
| The pumpkin anvil | 60 | 0 | 0 | |
| 2005 | Own-rooted tree | 60 | 31 | 50.2 |
| The pumpkin anvil | 60 | 0 | 0 |
The main proterties contrast table of the different graft stock cucumber of table 4
| The stock kind | The transplanting phase (moon/day) | Plant the climing number of blade of back 30 God | Sit the melon time (moon/day) | Beginning adopts the time (moon/day) | Single melon heavy (g) | Taste | Remarks |
| The black seed pumpkin anvil | 3/15 | 18 | 4/20 | 5/5 | 190 | Peculiar smell is arranged | |
| Own-rooted tree | 3/15 | 12 | 4/20 | 5/3 | 160 | Free from extraneous odour | |
| The pumpkin anvil | 3/15 | 25 | 4/15 | 4/29 | 220 | Free from extraneous odour | The sugar-preserved gourd direct light is bright |
The different graft stock muskmelon of the table 5 fusarium wilt incidence table of comparisons
| Time | The stock kind | The strain number | The withered strain number of dying of illness | Dead plant rate (%) |
| 2004 | Own-rooted tree | 100 | 49 | 49 |
| The pumpkin anvil | 100 | 0 | 0 | |
| 2005 | Own-rooted tree | 100 | 51 | 51 |
| The pumpkin anvil | 100 | 0 | 0 |
The main proterties contrast table of the different graft stock muskmelons of table 6
| The stock kind | The transplanting phase (moon/day) | Plant the climing number of blade of back 30 God | Sit the melon time (moon/day) | Beginning adopts the time (moon/day) | Single melon heavy (kg) | The center pol | The edge pol | Taste |
| Own-rooted tree | 2/11 | 15 | 3/23 | 4/27 | 0.6 | 14 | 13 | Free from extraneous odour |
| The pumpkin anvil | 2/11 | 27 | 3/16 | 4/22 | 1.3 | 18 | 16.5 | Free from extraneous odour |
In present specification, " YN " representative circle pumpkin among the YN-3, the long pumpkin of " CN " representative among the CN-3.
Claims (1)
1, a kind of producing method for seed of pumpkin rootstock, it is characterized in that with select to be grown in China's circle pumpkin in Shandong, through the selfing separation and purification of at least 3 generations, evaluation and screening goes out the inbred line that plant strain growth is sane under the low temperature and poor light condition, lower temperature resistance is strong (YN-3) for maternal, with select to be grown in the long pumpkin of India in Shandong, through the selfing separation and purification of at least 3 generations, that evaluation and screening goes out is low temperature resistant, drought resistance and the strong inbred line (CN-3) of disease resistance are male parent, hybridizes first generation cross-fertilize seed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610068737 CN1918970A (en) | 2006-09-04 | 2006-09-04 | Seeds production method for pumpkin rootstock |
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|---|---|---|---|
| CN 200610068737 CN1918970A (en) | 2006-09-04 | 2006-09-04 | Seeds production method for pumpkin rootstock |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138392A (en) * | 2010-01-28 | 2011-08-03 | 付景兴 | Method of cultivation frame type management in melon grafting and seedling shed |
| CN102893812A (en) * | 2012-10-11 | 2013-01-30 | 青岛金妈妈农业科技有限公司 | Breeding method for low temperature-resistant and wax powder-removing new species of cucumber rootstocks |
| CN103355162A (en) * | 2012-04-09 | 2013-10-23 | 辽宁省农业科学院蔬菜研究所 | Breeding method for vineless pumpkins for stocks |
| CN103688848A (en) * | 2013-12-16 | 2014-04-02 | 青岛金妈妈农业科技有限公司 | Three-way cross hybrid breeding method of cucumber root stock |
| CN104429632A (en) * | 2014-11-06 | 2015-03-25 | 舒城县汤池镇昌涛生态蔬菜种植专业合作社 | Efficient cultivation method for cucumber seedling grafting |
| CN104969776A (en) * | 2014-04-03 | 2015-10-14 | 济南伟丽种业有限公司 | Intensification root-breaking grafting and seedling cultivating method for thin-skinned melons |
| CN106358771A (en) * | 2016-08-31 | 2017-02-01 | 宿州市埇桥区九里梦种植专业合作社 | Grafting method for preventing graft union gumming disease of fruit cucumbers |
| CN106852247A (en) * | 2016-07-25 | 2017-06-16 | 江苏省农业科学院蔬菜研究所 | Planting method for improving commodity quality of Shenqing series cucumbers |
| CN107114087A (en) * | 2017-04-22 | 2017-09-01 | 张家口市农业科学院 | A kind of Cucumber High-yield Culture method |
| CN110432016A (en) * | 2019-09-19 | 2019-11-12 | 江苏省农业科学院 | A kind of selection of Grafting watermelon stock |
| CN114885693A (en) * | 2022-06-07 | 2022-08-12 | 青岛金妈妈农业科技有限公司 | Method for obtaining excellent seeds of pumpkin rootstock for powdery mildew-resistant cucumbers |
-
2006
- 2006-09-04 CN CN 200610068737 patent/CN1918970A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138392A (en) * | 2010-01-28 | 2011-08-03 | 付景兴 | Method of cultivation frame type management in melon grafting and seedling shed |
| CN103355162A (en) * | 2012-04-09 | 2013-10-23 | 辽宁省农业科学院蔬菜研究所 | Breeding method for vineless pumpkins for stocks |
| CN102893812B (en) * | 2012-10-11 | 2016-03-23 | 青岛金妈妈农业科技有限公司 | The selection of low temperature resistant dewaxing powder cucumber stock new varieties |
| CN102893812A (en) * | 2012-10-11 | 2013-01-30 | 青岛金妈妈农业科技有限公司 | Breeding method for low temperature-resistant and wax powder-removing new species of cucumber rootstocks |
| CN103688848A (en) * | 2013-12-16 | 2014-04-02 | 青岛金妈妈农业科技有限公司 | Three-way cross hybrid breeding method of cucumber root stock |
| CN103688848B (en) * | 2013-12-16 | 2016-08-17 | 青岛金妈妈农业科技有限公司 | A kind of triple hybrid selection of cucumber stock |
| CN104969776A (en) * | 2014-04-03 | 2015-10-14 | 济南伟丽种业有限公司 | Intensification root-breaking grafting and seedling cultivating method for thin-skinned melons |
| CN104429632A (en) * | 2014-11-06 | 2015-03-25 | 舒城县汤池镇昌涛生态蔬菜种植专业合作社 | Efficient cultivation method for cucumber seedling grafting |
| CN106852247A (en) * | 2016-07-25 | 2017-06-16 | 江苏省农业科学院蔬菜研究所 | Planting method for improving commodity quality of Shenqing series cucumbers |
| CN106358771A (en) * | 2016-08-31 | 2017-02-01 | 宿州市埇桥区九里梦种植专业合作社 | Grafting method for preventing graft union gumming disease of fruit cucumbers |
| CN107114087A (en) * | 2017-04-22 | 2017-09-01 | 张家口市农业科学院 | A kind of Cucumber High-yield Culture method |
| CN110432016A (en) * | 2019-09-19 | 2019-11-12 | 江苏省农业科学院 | A kind of selection of Grafting watermelon stock |
| CN114885693A (en) * | 2022-06-07 | 2022-08-12 | 青岛金妈妈农业科技有限公司 | Method for obtaining excellent seeds of pumpkin rootstock for powdery mildew-resistant cucumbers |
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