CN1198924C - 在丝状真菌中表达克隆 - Google Patents

在丝状真菌中表达克隆 Download PDF

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Publication number
CN1198924C
CN1198924C CN98812563.3A CN98812563A CN1198924C CN 1198924 C CN1198924 C CN 1198924C CN 98812563 A CN98812563 A CN 98812563A CN 1198924 C CN1198924 C CN 1198924C
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China
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cdna
dna
expression
gene
vector
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Expired - Lifetime
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CN98812563.3A
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English (en)
Chinese (zh)
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CN1283227A (zh
Inventor
J·M·范丹布林克
G·C·M·塞尔坦
J·P·T·W·范丹弘波夫
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Koninklijke DSM NV
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DSM NV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Mushroom Cultivation (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CN98812563.3A 1997-12-22 1998-12-22 在丝状真菌中表达克隆 Expired - Lifetime CN1198924C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97204079.4 1997-12-22
EP97204079 1997-12-22

Publications (2)

Publication Number Publication Date
CN1283227A CN1283227A (zh) 2001-02-07
CN1198924C true CN1198924C (zh) 2005-04-27

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ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98812563.3A Expired - Lifetime CN1198924C (zh) 1997-12-22 1998-12-22 在丝状真菌中表达克隆

Country Status (13)

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US (1) US20020155536A1 (enExample)
EP (2) EP1042461B1 (enExample)
JP (3) JP4410413B2 (enExample)
CN (1) CN1198924C (enExample)
AT (1) ATE367439T1 (enExample)
AU (1) AU736111C (enExample)
BR (1) BR9814404A (enExample)
CA (1) CA2313445C (enExample)
DE (1) DE69838106T2 (enExample)
DK (1) DK1042461T3 (enExample)
ES (1) ES2287989T3 (enExample)
PL (1) PL341760A1 (enExample)
WO (1) WO1999032617A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286483B (zh) * 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子

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CN100558898C (zh) 2000-09-21 2009-11-11 巴斯福股份公司 篮霉菌木聚糖酶
KR100867059B1 (ko) 2000-12-07 2008-11-04 디에스엠 아이피 어셋츠 비.브이. 카르복시 말단 프롤린 잔기를 갖는 펩티드가 풍부한단백질 가수분해물
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EP1799822A2 (en) * 2004-10-15 2007-06-27 DSMIP Assets B.V. Amidases from aspergillus niger and their use in a food production process
EP2410048B1 (en) 2005-01-24 2016-08-10 DSM IP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
US20090209007A1 (en) * 2005-03-18 2009-08-20 Roelf Bernhard Meima Method of Expression Cloning in a Host Cell
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JP2010517587A (ja) 2007-02-15 2010-05-27 ディーエスエム アイピー アセッツ ビー．ブイ． 関心のある化合物を生産するための組み換え宿主細胞
EP2113027B1 (en) 2007-02-20 2014-06-18 DSM IP Assets B.V. Novel sialidase
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MX346700B (es) 2009-03-10 2017-03-28 Dsm Ip Assets Bv Metodo para mejorar el rendimiento de un polipeptido.
DK2406372T3 (da) 2009-03-10 2017-11-27 Dsm Ip Assets Bv Prægastrisk esterase og derivater deraf
CN102414323B (zh) 2009-04-22 2015-07-08 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的重组多肽的方法
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US20150197760A1 (en) 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
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WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
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US9969988B2 (en) * 2014-01-31 2018-05-15 Api Corporation Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same
MX375798B (es) 2014-05-19 2025-03-07 Dsm Ip Assets Bv Endoproteasa específica para prolina.
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
US11396665B2 (en) 2015-01-06 2022-07-26 Dsm Ip Assets B.V. CRISPR-CAS system for a filamentous fungal host cell
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
AU2016273208B2 (en) 2015-06-02 2021-01-21 Dsm Ip Assets B.V. Use of ice structuring protein AFP19 expressed in filamentous fungal strains for preparing food
EP4361240A3 (en) 2015-11-17 2024-07-31 DSM IP Assets B.V. Preparation of a stable beer
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
RS60238B1 (sr) * 2016-09-20 2020-06-30 Clariant Int Ltd Postupak za selektivnu ekspresiju, nezavisnu od izvora ugljenika, sekvenci koje kodiraju proteine u ćeliji filamentozne gljive
EP3516056B1 (en) 2016-09-23 2024-11-27 DSM IP Assets B.V. A guide-rna expression system for a host cell
EP3559222A1 (en) 2016-12-21 2019-10-30 DSM IP Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US10889807B2 (en) 2016-12-21 2021-01-12 Dsm Ip Assets B.V. Lipolytic enzyme variants
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EP3607071A1 (en) 2017-04-06 2020-02-12 DSM IP Assets B.V. Self-guiding integration construct (sgic)
CN112424326A (zh) 2018-05-07 2021-02-26 帝斯曼知识产权资产管理有限公司 用于酶促油脱胶的方法
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102286483B (zh) * 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子

Also Published As

Publication number Publication date
ES2287989T3 (es) 2007-12-16
DE69838106T2 (de) 2008-04-03
EP1042461A2 (en) 2000-10-11
EP1854890A1 (en) 2007-11-14
JP2002509698A (ja) 2002-04-02
WO1999032617A2 (en) 1999-07-01
JP2005261438A (ja) 2005-09-29
CN1283227A (zh) 2001-02-07
EP1042461B1 (en) 2007-07-18
CA2313445C (en) 2012-03-20
AU2515199A (en) 1999-07-12
JP2010046080A (ja) 2010-03-04
WO1999032617A3 (en) 1999-09-10
US20020155536A1 (en) 2002-10-24
ATE367439T1 (de) 2007-08-15
JP4410413B2 (ja) 2010-02-03
AU736111B2 (en) 2001-07-26
AU736111C (en) 2004-02-19
PL341760A1 (en) 2001-05-07
DK1042461T3 (da) 2007-09-24
DE69838106D1 (de) 2007-08-30
BR9814404A (pt) 2000-10-10
CA2313445A1 (en) 1999-07-01

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