CA2313445C - Expression cloning in filamentous fungi - Google Patents
Expression cloning in filamentous fungi Download PDFInfo
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- CA2313445C CA2313445C CA2313445A CA2313445A CA2313445C CA 2313445 C CA2313445 C CA 2313445C CA 2313445 A CA2313445 A CA 2313445A CA 2313445 A CA2313445 A CA 2313445A CA 2313445 C CA2313445 C CA 2313445C
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97204079.4 | 1997-12-22 | ||
| EP97204079 | 1997-12-22 | ||
| PCT/EP1998/008577 WO1999032617A2 (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
Publications (2)
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| CA2313445A1 CA2313445A1 (en) | 1999-07-01 |
| CA2313445C true CA2313445C (en) | 2012-03-20 |
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|---|---|---|---|
| CA2313445A Expired - Fee Related CA2313445C (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
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|---|---|
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| EP (2) | EP1042461B1 (enExample) |
| JP (3) | JP4410413B2 (enExample) |
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| CA (1) | CA2313445C (enExample) |
| DE (1) | DE69838106T2 (enExample) |
| DK (1) | DK1042461T3 (enExample) |
| ES (1) | ES2287989T3 (enExample) |
| PL (1) | PL341760A1 (enExample) |
| WO (1) | WO1999032617A2 (enExample) |
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| CN100558898C (zh) | 2000-09-21 | 2009-11-11 | 巴斯福股份公司 | 篮霉菌木聚糖酶 |
| KR100867059B1 (ko) | 2000-12-07 | 2008-11-04 | 디에스엠 아이피 어셋츠 비.브이. | 카르복시 말단 프롤린 잔기를 갖는 펩티드가 풍부한단백질 가수분해물 |
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| AU2003219956A1 (en) * | 2003-02-27 | 2004-09-28 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
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| US20090209007A1 (en) * | 2005-03-18 | 2009-08-20 | Roelf Bernhard Meima | Method of Expression Cloning in a Host Cell |
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| JP6468561B2 (ja) | 2012-07-19 | 2019-02-13 | ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． | ＡｇｓＥ欠損株 |
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| WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| ES2706742T3 (es) | 2013-12-02 | 2019-04-01 | Dsm Ip Assets Bv | Proteína estructuradora del hielo |
| US9969988B2 (en) * | 2014-01-31 | 2018-05-15 | Api Corporation | Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same |
| MX375798B (es) | 2014-05-19 | 2025-03-07 | Dsm Ip Assets Bv | Endoproteasa específica para prolina. |
| WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| US11396665B2 (en) | 2015-01-06 | 2022-07-26 | Dsm Ip Assets B.V. | CRISPR-CAS system for a filamentous fungal host cell |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| AU2016273208B2 (en) | 2015-06-02 | 2021-01-21 | Dsm Ip Assets B.V. | Use of ice structuring protein AFP19 expressed in filamentous fungal strains for preparing food |
| EP4361240A3 (en) | 2015-11-17 | 2024-07-31 | DSM IP Assets B.V. | Preparation of a stable beer |
| US10913938B2 (en) | 2016-07-29 | 2021-02-09 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| RS60238B1 (sr) * | 2016-09-20 | 2020-06-30 | Clariant Int Ltd | Postupak za selektivnu ekspresiju, nezavisnu od izvora ugljenika, sekvenci koje kodiraju proteine u ćeliji filamentozne gljive |
| EP3516056B1 (en) | 2016-09-23 | 2024-11-27 | DSM IP Assets B.V. | A guide-rna expression system for a host cell |
| EP3559222A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| US10889807B2 (en) | 2016-12-21 | 2021-01-12 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| EP3596199A1 (en) | 2017-03-13 | 2020-01-22 | DSM IP Assets B.V. | Zinc binuclear cluster transcriptional regulator-deficient strain |
| EP3607071A1 (en) | 2017-04-06 | 2020-02-12 | DSM IP Assets B.V. | Self-guiding integration construct (sgic) |
| CN112424326A (zh) | 2018-05-07 | 2021-02-26 | 帝斯曼知识产权资产管理有限公司 | 用于酶促油脱胶的方法 |
| MX2020014191A (es) | 2018-06-19 | 2021-03-09 | Dsm Ip Assets Bv | Variantes de enzimas lipoliticas. |
| US20220389458A1 (en) | 2019-11-04 | 2022-12-08 | Dsm Ip Assets B.V. | Low volume transfection |
| JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
| WO2022269549A2 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
| JP2025520009A (ja) | 2022-05-16 | 2025-07-01 | ディーエスエム アイピー アセッツ ビー．ブイ． | 脂肪分解酵素変異体 |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
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| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| JP3153234B2 (ja) * | 1989-06-16 | 2001-04-03 | ジェネンコー インコーポレーテッド | 糸状菌からの目的とするポリペプチドの分泌を増幅するｄｎａ配列、ベクター、及び融合ポリペプチド |
| WO1991000920A2 (en) * | 1989-07-07 | 1991-01-24 | Unilever N.V. | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
| JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子 |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
| EP1321523A3 (en) * | 1993-07-23 | 2004-03-03 | DSM IP Assets B.V. | Selection marker gene free recombinant strains; a method for obtaining them and the use of these strains |
| JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Ｄｎａ修復酵素 |
| JPH11504218A (ja) * | 1995-04-24 | 1999-04-20 | クロマゾーム コーポレーション | 新規代謝経路の生成およびスクリーニングのための方法 |
| JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーｄｎａ塩基配列およびその用途 |
| DE69733059T2 (de) * | 1996-01-10 | 2006-03-02 | Novozymes A/S | Methode zur invivo produktion einer mutanten-bibliothek in zellen |
-
1998
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 EP EP98966868A patent/EP1042461B1/en not_active Revoked
- 1998-12-22 EP EP07112086A patent/EP1854890A1/en not_active Withdrawn
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en not_active Ceased
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
-
2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
-
2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
-
2009
- 2009-10-20 JP JP2009241780A patent/JP2010046080A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES2287989T3 (es) | 2007-12-16 |
| DE69838106T2 (de) | 2008-04-03 |
| EP1042461A2 (en) | 2000-10-11 |
| EP1854890A1 (en) | 2007-11-14 |
| JP2002509698A (ja) | 2002-04-02 |
| WO1999032617A2 (en) | 1999-07-01 |
| JP2005261438A (ja) | 2005-09-29 |
| CN1283227A (zh) | 2001-02-07 |
| EP1042461B1 (en) | 2007-07-18 |
| AU2515199A (en) | 1999-07-12 |
| JP2010046080A (ja) | 2010-03-04 |
| WO1999032617A3 (en) | 1999-09-10 |
| US20020155536A1 (en) | 2002-10-24 |
| ATE367439T1 (de) | 2007-08-15 |
| JP4410413B2 (ja) | 2010-02-03 |
| AU736111B2 (en) | 2001-07-26 |
| AU736111C (en) | 2004-02-19 |
| PL341760A1 (en) | 2001-05-07 |
| DK1042461T3 (da) | 2007-09-24 |
| DE69838106D1 (de) | 2007-08-30 |
| BR9814404A (pt) | 2000-10-10 |
| CN1198924C (zh) | 2005-04-27 |
| CA2313445A1 (en) | 1999-07-01 |
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