CN1161469C - 在细胞培养中增强干扰素生产的方法 - Google Patents

在细胞培养中增强干扰素生产的方法 Download PDF

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CN1161469C
CN1161469C CNB961977515A CN96197751A CN1161469C CN 1161469 C CN1161469 C CN 1161469C CN B961977515 A CNB961977515 A CN B961977515A CN 96197751 A CN96197751 A CN 96197751A CN 1161469 C CN1161469 C CN 1161469C
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interferon
cells
pkr
alpha
progeny
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CN1200148A (zh
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S
阿兰·S·劳
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University of California San Diego UCSD
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
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    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16641—Use of virus, viral particle or viral elements as a vector
    • C12N2710/16643—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32051—Methods of production or purification of viral material

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  • Plant Pathology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CNB961977515A 1995-08-22 1996-08-22 在细胞培养中增强干扰素生产的方法 Expired - Fee Related CN1161469C (zh)

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US262195P 1995-08-22 1995-08-22
US60/002,621 1995-08-22

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CN1200148A CN1200148A (zh) 1998-11-25
CN1161469C true CN1161469C (zh) 2004-08-11

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US (6) US5840565A (enExample)
EP (2) EP0846160B1 (enExample)
JP (2) JP4267693B2 (enExample)
CN (1) CN1161469C (enExample)
AT (1) ATE289348T1 (enExample)
AU (2) AU6857696A (enExample)
BR (1) BR9610550A (enExample)
CA (2) CA2229405C (enExample)
DE (1) DE69634360T2 (enExample)
WO (2) WO1997008324A1 (enExample)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840565A (en) * 1995-08-22 1998-11-24 The Regents Of The University Of California Methods for enhancing the production of viral vaccines in PKR-deficient cell culture
US6855519B2 (en) * 1995-08-22 2005-02-15 The Regents Of The University Of California Methods for enhancing the production of interferon in cell culture
US6740519B1 (en) 1997-11-03 2004-05-25 Mount Sinai School Of Medicine Immortalized, homozygous stat1-deficient mammalian cell lines and their uses
JP4837827B2 (ja) 1998-06-12 2011-12-14 マウント シナイ スクール オブ メディシン 新規なウイルス増殖方法およびそのためのインターフェロン欠損培養基
US6762038B1 (en) * 1998-09-09 2004-07-13 The Cleveland Clinic Foundation Mutant cell lines and methods for producing enhanced levels of recombinant proteins
HK1048650A1 (zh) * 1999-06-15 2003-04-11 Genetrol Biotherapeutics 在细胞培养物中增强细胞激动素(cytokines)的方法
EP1067179A1 (en) * 1999-07-09 2001-01-10 Pierre Fabre Medicament Method to select attenuated paramyxoviridae useful for vaccines and therapeutics
HK1048135A1 (zh) * 1999-09-08 2003-03-21 Genetrol Biotherapeutics 具增强细胞活力功能的高产量的细胞变动素(cytokine)
US20010031859A1 (en) * 1999-09-08 2001-10-18 Lau Allan S. High level cytokine production with enhanced cell viability
WO2001077394A1 (en) 2000-04-10 2001-10-18 Mount Sinai School Of Medicine Of New York University Screening methods for identifying viral proteins with interferon antagonizing functions and potential antiviral agents
IL136232A0 (en) * 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
US6660471B2 (en) * 2000-08-04 2003-12-09 Chiron Corporation Enhanced replication of HCV RNA
US20020150541A1 (en) * 2000-09-12 2002-10-17 Gene Trol Biotherapeutics, Inc. Compositions comprising mixtures of therapeutic proteins and methods of producing the same
AU2001290855A1 (en) 2000-09-14 2002-03-26 Genetrol Biotherapeutics, Inc. Method and cell composition for screening compounds for anti-inflammatory activity
AU2002251714A1 (en) * 2000-12-19 2002-08-19 Genetrol Biotherapeutics, Inc. Cells with enhanced pkr expression for increasing cytokine production
DE10104094C1 (de) * 2001-01-31 2002-07-25 Angelika Vallbracht-Flehmig Verfahren zur Erhöhung der Virusvermehrung und Virusausbeute in Zellkulturen, durch Blockade der RNaseL-Aktivierung
EP2332651A3 (en) * 2001-10-25 2011-08-31 Bar Ilan University Interactive transparent individual cells biochip processor
IL154677A0 (en) * 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
WO2004113492A1 (en) * 2003-06-26 2004-12-29 Molecular Cytomics Ltd. Improved materials for constructing cell-chips, cell-chip covers, cell-chip coats, processed cell-chips and uses thereof
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US8597597B2 (en) 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
WO2005008626A1 (en) * 2003-07-11 2005-01-27 University Of North Carolina At Chapel Hill Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop
KR101280094B1 (ko) * 2003-08-11 2013-06-28 사이단호진한다이비세이부쯔뵤우겐큐우카이 점막 면역을 유도할 수 있는 보강제를 함유한 신규한 백신
US20050064524A1 (en) * 2003-08-11 2005-03-24 Mordechai Deutsch Population of cells utilizable for substance detection and methods and devices using same
NZ591879A (en) * 2003-10-23 2012-11-30 Kineta Two Llc Detection of mutations in a gene associated with resistance to viral infection, OAS1
KR20060116825A (ko) 2003-10-23 2006-11-15 일루미겐 바이오사이언시스, 인코포레이티드 바이러스 감염에 대한 저항성과 관련된 유전자인ｏａｓ1에서의 돌연변이의 검출
CN103755800B (zh) 2004-02-02 2016-02-24 Ambrx公司 经修饰的人类生长激素多肽与其用途
BRPI0506610A (pt) * 2004-02-03 2007-05-02 Chemagis Ltd montelucaste sódico amorfo, processo de preparação de montelucaste sódico amorfo, composição farmacêutica, método de tratamento utilizando a administração de montelucaste sódico amorfo, co-precipitado amorfo de montelucaste sódico e processo de preparação de co-precipitado amorfo de montelucaste sódico
ES2329607T3 (es) * 2004-02-23 2009-11-27 Crucell Holland B.V. Metodos de purificacion de virus.
BR122015032743B1 (pt) 2004-06-01 2018-04-03 Mount Sinai School Of Medicine Of New York University Vírus atenuado de influenza de suínos geneticamente engenheirado, formulação imunogênica, formulação farmacêutica, seus usos e métodos para produzir uma vacina
US20080063251A1 (en) * 2004-07-07 2008-03-13 Mordechai Deutsch Method and Device for Identifying an Image of a Well in an Image of a Well-Bearing
US7403647B2 (en) * 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
WO2006021959A2 (en) * 2004-08-25 2006-03-02 Seng Enterprises Ltd. Method and device for isolating cells
US20060051369A1 (en) * 2004-08-27 2006-03-09 Taylor Deborah R ADAR1 antiviral pathway
US20080107683A1 (en) * 2004-11-30 2008-05-08 David Hone Bacterial Packaging Strains Useful for Generation and Production of Recombinant Double-Stranded RNA Nucleocapsids and Uses Thereof
DE602005023534D1 (de) 2005-01-25 2010-10-21 Seng Entpr Ltd Mikrofluidische vorrichtung zur untersuchung zellen
EP1855713B1 (en) 2005-02-15 2016-04-27 Mount Sinai School of Medicine Genetically engineered equine influenza virus and uses thereof
US8574595B2 (en) * 2005-04-11 2013-11-05 Crucell Holland B.V. Virus purification using ultrafiltration
UA95446C2 (ru) * 2005-05-04 2011-08-10 Іллюміджен Байосайєнсіз, Інк. Мутаци в генах oas1
DE102005034043B4 (de) * 2005-07-18 2019-12-12 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Gemisch, enthaltend L-Carnitin und Trehalulose, sowie Produkt enthaltend das Gemisch
US20070111309A1 (en) * 2005-10-04 2007-05-17 Daelli Marcelo G Vero cell line adapted to grow in suspension
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
WO2007052245A1 (en) 2005-11-03 2007-05-10 Seng Enterprises Ltd. Method and device for studying floating, living cells
US20070196333A1 (en) * 2006-02-23 2007-08-23 Fu-Yung Lin Composition comprising mixtures of IFN-alpha subtypes
US20060223999A1 (en) * 2006-05-10 2006-10-05 Chemagis Ltd. Process for preparing montelukast and precursors thereof
KR20100019467A (ko) 2007-05-02 2010-02-18 암브룩스, 인코포레이티드 변형된 인터페론 베타 폴리펩티드 및 그의 용도
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US9975118B2 (en) 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
NZ602170A (en) 2008-02-08 2014-03-28 Ambrx Inc Modified leptin polypeptides and their uses
EP2133358A1 (en) * 2008-06-10 2009-12-16 Universitätsklinikum Freiburg Rift valley fever virus-like particles and their use for immunization and as test system
KR101707569B1 (ko) * 2008-08-01 2017-02-16 감마 백신즈 피티와이 리미티드 인플루엔자 백신
CN102257135B (zh) 2008-12-16 2017-06-09 纳米医疗公司 流感疫苗的生产
JP2013523175A (ja) 2010-04-14 2013-06-17 イーエムディー・ミリポア・コーポレーション 高力価、高純度のウイルスストックの作製方法及びその使用方法
US20140079732A1 (en) 2011-01-27 2014-03-20 Gamma Vaccines Pty Limited Combination vaccines
EP2855701A1 (en) * 2012-06-04 2015-04-08 Novartis AG Improved safety testing
WO2014123967A2 (en) * 2013-02-05 2014-08-14 University Of Georgia Research Foundation, Inc. Cell lines for virus production and methods of use
EP3261664A1 (en) 2015-02-26 2018-01-03 Boehringer Ingelheim Vetmedica GmbH Bivalent swine influenza virus vaccine
WO2017070167A1 (en) 2015-10-20 2017-04-27 The University Of North Carolina At Chapel Hill Methods and compositions for modified factor ix fusion proteins
KR102651055B1 (ko) 2016-03-24 2024-03-26 삼성디스플레이 주식회사 광학 필름 및 디스플레이 장치

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE33164E (en) * 1979-05-15 1990-02-13 Mobay Corporation Influenza vaccine production in liquid cell culture
JPS5754586A (en) * 1980-09-18 1982-04-01 Ajinomoto Co Inc Cell strain producing human interferon prolongably, its preparation, and preparation of human interferon using it
US4579821A (en) * 1981-11-23 1986-04-01 University Patents, Inc. Control of DNA sequence transcription
JPS58209993A (ja) * 1982-05-31 1983-12-07 Mochida Pharmaceut Co Ltd インタ−フエロンの産生方法
ATE55620T1 (de) * 1982-06-21 1990-09-15 Wellcome Found Verfahren zur herstellung von interferon.
US4485038A (en) * 1982-10-19 1984-11-27 Health Research (Roswell Park Division) Method for the production and purification of human leukocyte interferon
US4966843A (en) * 1982-11-01 1990-10-30 Cetus Corporation Expression of interferon genes in Chinese hamster ovary cells
US4745053A (en) * 1983-07-08 1988-05-17 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyojo Process for producing human interferon and method for assaying the interferon productivity of blood
JPS6062997A (ja) * 1983-09-17 1985-04-11 Mochida Pharmaceut Co Ltd インタ−フエロンの生産方法
US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
US4732683A (en) * 1986-12-02 1988-03-22 Biospectrum, Inc. Purification method for alpha interferon
ES2110411T3 (es) * 1989-08-25 1998-02-16 Chiron Corp Metodos para cultivar el virus de la hepatitis c (hcv) en lineas celulares de linfocitos b o t.
US5149531A (en) * 1990-06-27 1992-09-22 University Of Pittsburgh Of The Commonwealth System Of Higher Education Method of using cold-adapted live influenza virus vaccine as an antiviral agent against influenza
JPH05260981A (ja) * 1992-03-02 1993-10-12 Massachusetts Inst Of Technol <Mit> ヘムに調節される真核細胞開始因子２αキナーゼをコードするＤＮＡ
FR2689520B1 (fr) * 1992-04-03 1996-07-19 Bio Merieux Procede et milieu de culture pour l'obtention de cellules infectees par un virus associe a la sclerose en plaques.
US5840565A (en) * 1995-08-22 1998-11-24 The Regents Of The University Of California Methods for enhancing the production of viral vaccines in PKR-deficient cell culture

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EP0846160A1 (en) 1998-06-10
AU6860896A (en) 1997-03-19
CA2229405A1 (en) 1997-03-06
ATE289348T1 (de) 2005-03-15
US6159712A (en) 2000-12-12
CA2229163C (en) 2011-04-05
EP0846174A4 (en) 2003-02-05
US6686190B2 (en) 2004-02-03
EP0846160B1 (en) 2005-02-16
US7132271B2 (en) 2006-11-07
MX9801423A (es) 1998-05-31
DE69634360T2 (de) 2005-12-29
BR9610550A (pt) 1999-07-06
JP3432519B2 (ja) 2003-08-04
US6489144B1 (en) 2002-12-03
US6673591B2 (en) 2004-01-06
AU707130B2 (en) 1999-07-01
AU6857696A (en) 1997-03-19
CA2229163A1 (en) 1997-03-06
US20010001709A1 (en) 2001-05-24
US20010001290A1 (en) 2001-05-17
DE69634360D1 (de) 2005-03-24
JPH11514344A (ja) 1999-12-07
JPH11511324A (ja) 1999-10-05
US5840565A (en) 1998-11-24
EP0846160A4 (en) 2002-01-02
CA2229405C (en) 2004-03-02
US20040157310A1 (en) 2004-08-12
JP4267693B2 (ja) 2009-05-27
EP0846174A1 (en) 1998-06-10
CN1200148A (zh) 1998-11-25
WO1997008324A1 (en) 1997-03-06
WO1997008292A1 (en) 1997-03-06

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