CN114286865A - 检测变体核酸的解链温度方法、试剂盒和报告寡核苷酸 - Google Patents

检测变体核酸的解链温度方法、试剂盒和报告寡核苷酸 Download PDF

Info

Publication number
CN114286865A
CN114286865A CN202080035128.5A CN202080035128A CN114286865A CN 114286865 A CN114286865 A CN 114286865A CN 202080035128 A CN202080035128 A CN 202080035128A CN 114286865 A CN114286865 A CN 114286865A
Authority
CN
China
Prior art keywords
sequence
nucleotides
nucleic acid
reporter oligonucleotide
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080035128.5A
Other languages
English (en)
Chinese (zh)
Inventor
K·K·本迪森
S·K·埃里克森
E·E·汉森
U·B·克里斯滕森
R·K·彼得森
C·L·马德森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PENTABASE ApS
Original Assignee
PENTABASE ApS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PENTABASE ApS filed Critical PENTABASE ApS
Publication of CN114286865A publication Critical patent/CN114286865A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080035128.5A 2019-05-13 2020-05-13 检测变体核酸的解链温度方法、试剂盒和报告寡核苷酸 Pending CN114286865A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19174084 2019-05-13
EP19174084.4 2019-05-13
PCT/EP2020/063269 WO2020229510A1 (en) 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Publications (1)

Publication Number Publication Date
CN114286865A true CN114286865A (zh) 2022-04-05

Family

ID=66529868

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080035128.5A Pending CN114286865A (zh) 2019-05-13 2020-05-13 检测变体核酸的解链温度方法、试剂盒和报告寡核苷酸

Country Status (9)

Country Link
US (1) US20220205024A1 (https=)
EP (1) EP3969614A1 (https=)
JP (1) JP7681520B2 (https=)
KR (1) KR20220031544A (https=)
CN (1) CN114286865A (https=)
AU (1) AU2020275447B2 (https=)
CA (1) CA3137670A1 (https=)
SG (1) SG11202110814QA (https=)
WO (1) WO2020229510A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114464246A (zh) * 2022-01-19 2022-05-10 华中科技大学同济医学院附属协和医院 基于CovMutt框架检测与遗传性增加相关的突变的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20260035911A (ko) * 2023-09-08 2026-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
KR20250091512A (ko) * 2023-12-14 2025-06-23 주식회사 시선바이오머티리얼스 디지털 중합효소연쇄반응 기반 현미부수체 불안정성 진단을 위한 정보제공 방법 및 이를 위한 키트

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101448957A (zh) * 2006-03-16 2009-06-03 潘塔贝斯公司 用于检测核酸、核酸的甲基化状态和突变体的包含信号对和疏水性核苷酸、无茎信标的寡核苷酸
CN102459651A (zh) * 2009-06-15 2012-05-16 悉尼大学 测定对免疫调节组合物治疗的应答的方法
CN108350506A (zh) * 2015-09-22 2018-07-31 比奥卡尔齐斯股份有限公司 改进的短同聚重复的检测

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
WO2003052133A2 (en) 2001-12-18 2003-06-26 Human Genetic Signatures Pty Ltd Oligonucleotide analogues comprising intercalator pseudonucleotides
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
JP2010110235A (ja) 2008-11-04 2010-05-20 Tosoh Corp 核酸プローブを用いた核酸の変異検出方法
JP2011135823A (ja) * 2009-12-28 2011-07-14 Nagoya Univ オリゴヌクレオチドプローブ及びその利用
WO2017045689A1 (en) 2015-09-14 2017-03-23 Pentabase Aps Methods and materials for detection of mutations
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析
US11473126B2 (en) * 2017-03-24 2022-10-18 Seasunbio Materials Method for analyzing melting curve using bi-functional PNA probe, method for diagnosing microsatellite instability using the same, and kit for diagnosing microsatellite instability
EP3652336B1 (en) 2017-07-12 2024-04-17 Institut Curie Method for detecting a mutation in a microsatellite sequence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101448957A (zh) * 2006-03-16 2009-06-03 潘塔贝斯公司 用于检测核酸、核酸的甲基化状态和突变体的包含信号对和疏水性核苷酸、无茎信标的寡核苷酸
CN102459651A (zh) * 2009-06-15 2012-05-16 悉尼大学 测定对免疫调节组合物治疗的应答的方法
CN108350506A (zh) * 2015-09-22 2018-07-31 比奥卡尔齐斯股份有限公司 改进的短同聚重复的检测

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XIANG DONGSHAN等: "The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs", TALANTA, ELSEVIER, AMSTERDAM, NL, vol. 174, 8 June 2017 (2017-06-08), pages 289 - 294, XP085138315, DOI: 10.1016/j.talanta.2017.06.020 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114464246A (zh) * 2022-01-19 2022-05-10 华中科技大学同济医学院附属协和医院 基于CovMutt框架检测与遗传性增加相关的突变的方法

Also Published As

Publication number Publication date
AU2020275447B2 (en) 2026-01-29
JP7681520B2 (ja) 2025-05-22
AU2020275447A1 (en) 2021-10-28
EP3969614A1 (en) 2022-03-23
CA3137670A1 (en) 2020-11-19
US20220205024A1 (en) 2022-06-30
WO2020229510A1 (en) 2020-11-19
JP2022533088A (ja) 2022-07-21
SG11202110814QA (en) 2021-11-29
KR20220031544A (ko) 2022-03-11

Similar Documents

Publication Publication Date Title
JP4743787B2 (ja) Ｄｎａ中のシトシンのメチル化法および検出法
CN102242211A (zh) 检测突变核酸序列的方法及试剂盒
WO2007115242A2 (en) Binary probes for fluorescent analysis of nucleic acids
KR20140010093A (ko) 혼합 집단 중 표적 dna의 서열분석을 위한 키트 및 방법
JP2012040029A (ja) 変異の検出方法およびそれに用いるキット
JP7681520B2 (ja) 変異体核酸を検出するための融解温度法、キットおよびレポータオリゴ
US8877910B2 (en) Probe for detecting polymorphism in exon 12 of NPM1 gene and use thereof
Xiong et al. Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro
EP3350347B1 (en) Methods and materials for detection of mutations
CN101796185B (zh) 扩增甲基化核酸或非甲基化核酸的方法
EP2319922B1 (en) Nucleic acid probe set and method of using the same
JP2008182974A (ja) 核酸プローブセットおよびその使用方法
KR102438915B1 (ko) 표적 염기 배열을 검출하는 방법 프로브를 설계 및 제조하는 방법 및 키트
JP2012105644A (ja) 多型検出用プローブ、多型検出方法、薬効評価方法、および多型検出用キット
US6821733B2 (en) Methods and compositions for detecting differences between nucleic acids
US20100311609A1 (en) Methylation Profile of Neuroinflammatory Demyelinating Diseases
RU2821911C2 (ru) Способы детектирования вариантов нуклеиновых кислот
KR20200044511A (ko) 노인성 황반변성 관련 유전자 변이 판별용 프로브 및 이를 이용한 노인성 황반변성 관련 유전자 변이 판별방법
CN101983245B (zh) 判断是否已适当进行非甲基化胞嘧啶转化处理的试剂盒和方法及用其分析甲基化dna的方法
JP5687414B2 (ja) 多型の識別方法
Bruno et al. High-sensitive microarray substrates specifically designed to improve sensitivity for the identification of fetal paternally inherited sequences in maternal plasma
EP2620509A1 (en) Method for detecting mutant dna
EP4599081A1 (en) Detection of molecular analytes based on tailored probe competition
CN116209775A (zh) 甲基化状态的检测
Gunn et al. Biomarkers of early effect in the study of cancer risk

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination