AU2020275447B2 - Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids - Google Patents

Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Info

Publication number
AU2020275447B2
AU2020275447B2 AU2020275447A AU2020275447A AU2020275447B2 AU 2020275447 B2 AU2020275447 B2 AU 2020275447B2 AU 2020275447 A AU2020275447 A AU 2020275447A AU 2020275447 A AU2020275447 A AU 2020275447A AU 2020275447 B2 AU2020275447 B2 AU 2020275447B2
Authority
AU
Australia
Prior art keywords
sequence
nucleotides
nucleic acid
reporter oligonucleotide
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020275447A
Other languages
English (en)
Other versions
AU2020275447A1 (en
Inventor
Kamilla Kolding BENDIXEN
Ulf Bech Christensen
Sarah Kronborg ERIKSEN
Emeli Elisabeth HANSEN
Cesilie Lind MADSEN
Rasmus Koefoed PETERSEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PENTABASE ApS
Original Assignee
PENTABASE ApS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PENTABASE ApS filed Critical PENTABASE ApS
Publication of AU2020275447A1 publication Critical patent/AU2020275447A1/en
Application granted granted Critical
Publication of AU2020275447B2 publication Critical patent/AU2020275447B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2020275447A 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids Active AU2020275447B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19174084 2019-05-13
EP19174084.4 2019-05-13
PCT/EP2020/063269 WO2020229510A1 (en) 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Publications (2)

Publication Number Publication Date
AU2020275447A1 AU2020275447A1 (en) 2021-10-28
AU2020275447B2 true AU2020275447B2 (en) 2026-01-29

Family

ID=66529868

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020275447A Active AU2020275447B2 (en) 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Country Status (9)

Country Link
US (1) US20220205024A1 (https=)
EP (1) EP3969614A1 (https=)
JP (1) JP7681520B2 (https=)
KR (1) KR20220031544A (https=)
CN (1) CN114286865A (https=)
AU (1) AU2020275447B2 (https=)
CA (1) CA3137670A1 (https=)
SG (1) SG11202110814QA (https=)
WO (1) WO2020229510A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114464246B (zh) * 2022-01-19 2023-05-30 华中科技大学同济医学院附属协和医院 基于CovMutt框架检测与遗传性增加相关的突变的方法
KR20260035911A (ko) * 2023-09-08 2026-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
KR20250091512A (ko) * 2023-12-14 2025-06-23 주식회사 시선바이오머티리얼스 디지털 중합효소연쇄반응 기반 현미부수체 불안정성 진단을 위한 정보제공 방법 및 이를 위한 키트

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007104318A2 (en) * 2006-03-16 2007-09-20 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
WO2003052133A2 (en) 2001-12-18 2003-06-26 Human Genetic Signatures Pty Ltd Oligonucleotide analogues comprising intercalator pseudonucleotides
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
JP2010110235A (ja) 2008-11-04 2010-05-20 Tosoh Corp 核酸プローブを用いた核酸の変異検出方法
US20100316608A1 (en) * 2009-06-15 2010-12-16 Vijayaprakash Suppiah Method of Determining A Response To Treatment With Immunomodulatory Composition
JP2011135823A (ja) * 2009-12-28 2011-07-14 Nagoya Univ オリゴヌクレオチドプローブ及びその利用
WO2017045689A1 (en) 2015-09-14 2017-03-23 Pentabase Aps Methods and materials for detection of mutations
KR102648647B1 (ko) * 2015-09-22 2024-03-18 비오까르띠 엔브이 짧은 호모폴리머릭 반복서열의 개선된 검출법
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析
US11473126B2 (en) * 2017-03-24 2022-10-18 Seasunbio Materials Method for analyzing melting curve using bi-functional PNA probe, method for diagnosing microsatellite instability using the same, and kit for diagnosing microsatellite instability
EP3652336B1 (en) 2017-07-12 2024-04-17 Institut Curie Method for detecting a mutation in a microsatellite sequence

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007104318A2 (en) * 2006-03-16 2007-09-20 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids

Also Published As

Publication number Publication date
JP7681520B2 (ja) 2025-05-22
AU2020275447A1 (en) 2021-10-28
EP3969614A1 (en) 2022-03-23
CA3137670A1 (en) 2020-11-19
CN114286865A (zh) 2022-04-05
US20220205024A1 (en) 2022-06-30
WO2020229510A1 (en) 2020-11-19
JP2022533088A (ja) 2022-07-21
SG11202110814QA (en) 2021-11-29
KR20220031544A (ko) 2022-03-11

Similar Documents

Publication Publication Date Title
WO1995029251A1 (en) Detection of mutation by resolvase cleavage
WO2007115242A2 (en) Binary probes for fluorescent analysis of nucleic acids
AU2020275447B2 (en) Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids
US8877910B2 (en) Probe for detecting polymorphism in exon 12 of NPM1 gene and use thereof
EP3350347B1 (en) Methods and materials for detection of mutations
CN104928355A (zh) 检测braf基因突变的方法及其试剂盒
WO2007105673A1 (ja) 変異遺伝子の検出方法
CN101796185B (zh) 扩增甲基化核酸或非甲基化核酸的方法
JP5900908B2 (ja) 一塩基反復多型解析方法及び一塩基多型解析方法
JP2012105644A (ja) 多型検出用プローブ、多型検出方法、薬効評価方法、および多型検出用キット
KR102438915B1 (ko) 표적 염기 배열을 검출하는 방법 프로브를 설계 및 제조하는 방법 및 키트
KR101581388B1 (ko) 다형 검출용 프로브, 다형 검출 방법, 약효 판정 방법 및 다형 검출용 시약 키트
RU2821911C2 (ru) Способы детектирования вариантов нуклеиновых кислот
Zhang et al. Label-free genotyping of single-nucleotide polymorphisms for DNA and RNA targets using a cationic polythiophene derivate
JP5641465B2 (ja) 一塩基反復多型解析方法及び一塩基多型解析方法
US20130084564A1 (en) Assessment of cancer risk based on rnu2 cnv and interplay between rnu2 cnv and brca1
KR20180033911A (ko) 표적 핵산 또는 유전적 변이를 다중 검출하는 방법
Gunn et al. Biomarkers of early effect in the study of cancer risk
WO2009098998A1 (ja) 核酸検出方法及び核酸検出用キット
JP2010246492A (ja) 多型の識別方法
CN113272444A (zh) 基于消除探针的检测染色体数目异常的方法和用于检测染色体数目异常的核酸组合物
HK40048081A (en) Elimination probe-based method for detecting numerical chromosomal abnormalities, and nucleic acid composition for detecting numerical chromosomal abnormalities