SG11202110814QA - Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids - Google Patents

Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Info

Publication number
SG11202110814QA
SG11202110814QA SG11202110814QA SG11202110814QA SG11202110814QA SG 11202110814Q A SG11202110814Q A SG 11202110814QA SG 11202110814Q A SG11202110814Q A SG 11202110814QA SG 11202110814Q A SG11202110814Q A SG 11202110814QA SG 11202110814Q A SG11202110814Q A SG 11202110814QA
Authority
SG
Singapore
Prior art keywords
sequence
nucleotides
nucleic acid
reporter oligonucleotide
nucleotide
Prior art date
Application number
SG11202110814QA
Other languages
English (en)
Inventor
Kamilla Kolding Bendixen
Sarah Kronborg Eriksen
Emeli Elisabeth Hansen
Ulf Bech Christensen
Rasmus Koefoed Petersen
Cesilie Lind Madsen
Original Assignee
Pentabase Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pentabase Aps filed Critical Pentabase Aps
Publication of SG11202110814QA publication Critical patent/SG11202110814QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202110814QA 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids SG11202110814QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19174084 2019-05-13
PCT/EP2020/063269 WO2020229510A1 (en) 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Publications (1)

Publication Number Publication Date
SG11202110814QA true SG11202110814QA (en) 2021-11-29

Family

ID=66529868

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202110814QA SG11202110814QA (en) 2019-05-13 2020-05-13 Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids

Country Status (9)

Country Link
US (1) US20220205024A1 (https=)
EP (1) EP3969614A1 (https=)
JP (1) JP7681520B2 (https=)
KR (1) KR20220031544A (https=)
CN (1) CN114286865A (https=)
AU (1) AU2020275447B2 (https=)
CA (1) CA3137670A1 (https=)
SG (1) SG11202110814QA (https=)
WO (1) WO2020229510A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114464246B (zh) * 2022-01-19 2023-05-30 华中科技大学同济医学院附属协和医院 基于CovMutt框架检测与遗传性增加相关的突变的方法
WO2025053620A1 (ko) * 2023-09-08 2025-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
KR20250091512A (ko) * 2023-12-14 2025-06-23 주식회사 시선바이오머티리얼스 디지털 중합효소연쇄반응 기반 현미부수체 불안정성 진단을 위한 정보제공 방법 및 이를 위한 키트

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
AU2002358464B2 (en) 2001-12-18 2006-04-27 Human Genetic Signatures Pty Ltd Pseudonucleotide comprising an intercalator
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CA2645136C (en) 2006-03-16 2017-09-19 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
JP2010110235A (ja) 2008-11-04 2010-05-20 Tosoh Corp 核酸プローブを用いた核酸の変異検出方法
US20100316608A1 (en) * 2009-06-15 2010-12-16 Vijayaprakash Suppiah Method of Determining A Response To Treatment With Immunomodulatory Composition
JP2011135823A (ja) * 2009-12-28 2011-07-14 Nagoya Univ オリゴヌクレオチドプローブ及びその利用
EP3350347B1 (en) 2015-09-14 2021-01-20 Pentabase APS Methods and materials for detection of mutations
EP4299763A2 (en) * 2015-09-22 2024-01-03 Biocartis NV Improved detection of short homopolymeric repeats
WO2017123647A1 (en) * 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
US11473126B2 (en) 2017-03-24 2022-10-18 Seasunbio Materials Method for analyzing melting curve using bi-functional PNA probe, method for diagnosing microsatellite instability using the same, and kit for diagnosing microsatellite instability
US20200291466A1 (en) 2017-07-12 2020-09-17 Institut Curie Method for Detecting a Mutation in a Microsatellite Sequence

Also Published As

Publication number Publication date
KR20220031544A (ko) 2022-03-11
AU2020275447A1 (en) 2021-10-28
JP2022533088A (ja) 2022-07-21
CA3137670A1 (en) 2020-11-19
EP3969614A1 (en) 2022-03-23
JP7681520B2 (ja) 2025-05-22
AU2020275447B2 (en) 2026-01-29
CN114286865A (zh) 2022-04-05
US20220205024A1 (en) 2022-06-30
WO2020229510A1 (en) 2020-11-19

Similar Documents

Publication Publication Date Title
WO2007115242A2 (en) Binary probes for fluorescent analysis of nucleic acids
JP4743787B2 (ja) Ｄｎａ中のシトシンのメチル化法および検出法
AU2020275447B2 (en) Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids
JP2012040029A (ja) 変異の検出方法およびそれに用いるキット
US8877910B2 (en) Probe for detecting polymorphism in exon 12 of NPM1 gene and use thereof
EP3350347B1 (en) Methods and materials for detection of mutations
Yu et al. Novel assay for quantitative analysis of DNA methylation at single-base resolution
CN104928355A (zh) 检测braf基因突变的方法及其试剂盒
WO2007105673A1 (ja) 変異遺伝子の検出方法
Bae et al. Comprehensive detection of diverse exon 19 deletion mutations of EGFR in lung Cancer by a single probe set
US20220298557A1 (en) Method for designing a probe combination
JP5900908B2 (ja) 一塩基反復多型解析方法及び一塩基多型解析方法
RU2821911C2 (ru) Способы детектирования вариантов нуклеиновых кислот
KR20120124029A (ko) 다형 검출용 프로브, 다형 검출 방법, 약효 판정 방법 및 다형 검출용 시약 키트
JP5641465B2 (ja) 一塩基反復多型解析方法及び一塩基多型解析方法
US20130084564A1 (en) Assessment of cancer risk based on rnu2 cnv and interplay between rnu2 cnv and brca1
Gunn et al. Biomarkers of early effect in the study of cancer risk
JP2010246492A (ja) 多型の識別方法
WO2009098998A1 (ja) 核酸検出方法及び核酸検出用キット