KR20220031544A - 변이체 핵산 검출을 위한 용융 온도 방법, 키트 및 리포터 올리고 - Google Patents
변이체 핵산 검출을 위한 용융 온도 방법, 키트 및 리포터 올리고 Download PDFInfo
- Publication number
- KR20220031544A KR20220031544A KR1020217036168A KR20217036168A KR20220031544A KR 20220031544 A KR20220031544 A KR 20220031544A KR 1020217036168 A KR1020217036168 A KR 1020217036168A KR 20217036168 A KR20217036168 A KR 20217036168A KR 20220031544 A KR20220031544 A KR 20220031544A
- Authority
- KR
- South Korea
- Prior art keywords
- sequence
- nucleotides
- nucleic acid
- reporter oligonucleotide
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/117—Modifications characterised by incorporating modified base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19174084 | 2019-05-13 | ||
| EP19174084.4 | 2019-05-13 | ||
| PCT/EP2020/063269 WO2020229510A1 (en) | 2019-05-13 | 2020-05-13 | Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20220031544A true KR20220031544A (ko) | 2022-03-11 |
Family
ID=66529868
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217036168A Pending KR20220031544A (ko) | 2019-05-13 | 2020-05-13 | 변이체 핵산 검출을 위한 용융 온도 방법, 키트 및 리포터 올리고 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20220205024A1 (https=) |
| EP (1) | EP3969614A1 (https=) |
| JP (1) | JP7681520B2 (https=) |
| KR (1) | KR20220031544A (https=) |
| CN (1) | CN114286865A (https=) |
| AU (1) | AU2020275447B2 (https=) |
| CA (1) | CA3137670A1 (https=) |
| SG (1) | SG11202110814QA (https=) |
| WO (1) | WO2020229510A1 (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025127642A1 (ko) * | 2023-12-14 | 2025-06-19 | 주식회사 시선바이오머티리얼스 | 디지털 중합효소연쇄반응 기반 현미부수체 불안정성 진단을 위한 정보제공 방법 및 이를 위한 키트 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114464246B (zh) * | 2022-01-19 | 2023-05-30 | 华中科技大学同济医学院附属协和医院 | 基于CovMutt框架检测与遗传性增加相关的突变的方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6031098A (en) * | 1997-08-11 | 2000-02-29 | California Institute Of Technology | Detection and treatment of duplex polynucleotide damage |
| WO2003052133A2 (en) | 2001-12-18 | 2003-06-26 | Human Genetic Signatures Pty Ltd | Oligonucleotide analogues comprising intercalator pseudonucleotides |
| SG170028A1 (en) * | 2006-02-24 | 2011-04-29 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| US8133984B2 (en) | 2006-03-16 | 2012-03-13 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| JP2010110235A (ja) | 2008-11-04 | 2010-05-20 | Tosoh Corp | 核酸プローブを用いた核酸の変異検出方法 |
| US20100316608A1 (en) * | 2009-06-15 | 2010-12-16 | Vijayaprakash Suppiah | Method of Determining A Response To Treatment With Immunomodulatory Composition |
| JP2011135823A (ja) * | 2009-12-28 | 2011-07-14 | Nagoya Univ | オリゴヌクレオチドプローブ及びその利用 |
| WO2017045689A1 (en) | 2015-09-14 | 2017-03-23 | Pentabase Aps | Methods and materials for detection of mutations |
| KR102648647B1 (ko) * | 2015-09-22 | 2024-03-18 | 비오까르띠 엔브이 | 짧은 호모폴리머릭 반복서열의 개선된 검출법 |
| JP6967006B2 (ja) * | 2016-01-15 | 2021-11-17 | クアンタポール, インコーポレイテッド | 低減されたバックグラウンドの光学に基づくナノポア分析 |
| US11473126B2 (en) * | 2017-03-24 | 2022-10-18 | Seasunbio Materials | Method for analyzing melting curve using bi-functional PNA probe, method for diagnosing microsatellite instability using the same, and kit for diagnosing microsatellite instability |
| EP3652336B1 (en) | 2017-07-12 | 2024-04-17 | Institut Curie | Method for detecting a mutation in a microsatellite sequence |
-
2020
- 2020-05-13 SG SG11202110814QA patent/SG11202110814QA/en unknown
- 2020-05-13 AU AU2020275447A patent/AU2020275447B2/en active Active
- 2020-05-13 KR KR1020217036168A patent/KR20220031544A/ko active Pending
- 2020-05-13 CA CA3137670A patent/CA3137670A1/en active Pending
- 2020-05-13 US US17/610,878 patent/US20220205024A1/en active Pending
- 2020-05-13 WO PCT/EP2020/063269 patent/WO2020229510A1/en not_active Ceased
- 2020-05-13 CN CN202080035128.5A patent/CN114286865A/zh active Pending
- 2020-05-13 EP EP20724155.5A patent/EP3969614A1/en active Pending
- 2020-05-13 JP JP2021567881A patent/JP7681520B2/ja active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025127642A1 (ko) * | 2023-12-14 | 2025-06-19 | 주식회사 시선바이오머티리얼스 | 디지털 중합효소연쇄반응 기반 현미부수체 불안정성 진단을 위한 정보제공 방법 및 이를 위한 키트 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020275447B2 (en) | 2026-01-29 |
| JP7681520B2 (ja) | 2025-05-22 |
| AU2020275447A1 (en) | 2021-10-28 |
| EP3969614A1 (en) | 2022-03-23 |
| CA3137670A1 (en) | 2020-11-19 |
| CN114286865A (zh) | 2022-04-05 |
| US20220205024A1 (en) | 2022-06-30 |
| WO2020229510A1 (en) | 2020-11-19 |
| JP2022533088A (ja) | 2022-07-21 |
| SG11202110814QA (en) | 2021-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2852690B1 (en) | A QUANTITATIVE MULTIPLEX METHYLATION SPECIFIC PCR METHOD- cMethDNA, REAGENTS, AND ITS USE | |
| WO2007115242A2 (en) | Binary probes for fluorescent analysis of nucleic acids | |
| US20060110765A1 (en) | Detection of nucleic acid variation by cleavage-amplification (CleavAmp) method | |
| JP4743787B2 (ja) | Dna中のシトシンのメチル化法および検出法 | |
| JP2012040029A (ja) | 変異の検出方法およびそれに用いるキット | |
| JP7681520B2 (ja) | 変異体核酸を検出するための融解温度法、キットおよびレポータオリゴ | |
| Yu et al. | Novel assay for quantitative analysis of DNA methylation at single-base resolution | |
| EP3350347B1 (en) | Methods and materials for detection of mutations | |
| Scholl et al. | Rapid screening and sensitive detection of NPM1 (nucleophosmin) exon 12 mutations in acute myeloid leukaemia | |
| Bae et al. | Comprehensive detection of diverse exon 19 deletion mutations of EGFR in lung Cancer by a single probe set | |
| US20220298557A1 (en) | Method for designing a probe combination | |
| CN101796185B (zh) | 扩增甲基化核酸或非甲基化核酸的方法 | |
| JP2008182974A (ja) | 核酸プローブセットおよびその使用方法 | |
| WO2010013317A1 (ja) | 核酸プローブセットおよびその使用方法 | |
| US6821733B2 (en) | Methods and compositions for detecting differences between nucleic acids | |
| RU2821911C2 (ru) | Способы детектирования вариантов нуклеиновых кислот | |
| KR102575618B1 (ko) | 가이드 프로브 및 클램핑 프로브를 이용한 표적핵산 증폭방법 및 이를 포함하는 표적핵산 증폭용 조성물 | |
| US20100311609A1 (en) | Methylation Profile of Neuroinflammatory Demyelinating Diseases | |
| CN101983245B (zh) | 判断是否已适当进行非甲基化胞嘧啶转化处理的试剂盒和方法及用其分析甲基化dna的方法 | |
| JP2012213410A (ja) | 核酸プローブセットおよびその使用方法 | |
| JP5687414B2 (ja) | 多型の識別方法 | |
| US20130084564A1 (en) | Assessment of cancer risk based on rnu2 cnv and interplay between rnu2 cnv and brca1 | |
| Gunn et al. | Biomarkers of early effect in the study of cancer risk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
|
| R18-X000 | Changes to party contact information recorded |
St.27 status event code: A-3-3-R10-R18-oth-X000 |
|
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |
|
| D21 | Rejection of application intended |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |