CN114236119A - 新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 - Google Patents
新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 Download PDFInfo
- Publication number
- CN114236119A CN114236119A CN202111316008.5A CN202111316008A CN114236119A CN 114236119 A CN114236119 A CN 114236119A CN 202111316008 A CN202111316008 A CN 202111316008A CN 114236119 A CN114236119 A CN 114236119A
- Authority
- CN
- China
- Prior art keywords
- colloidal gold
- antibody
- pad
- solution
- test strip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 238000001514 detection method Methods 0.000 title claims abstract description 42
- 238000012360 testing method Methods 0.000 title claims abstract description 27
- 230000003472 neutralizing effect Effects 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 241000711573 Coronaviridae Species 0.000 claims abstract description 25
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000003908 quality control method Methods 0.000 claims abstract description 12
- 241000283707 Capra Species 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 210000004369 blood Anatomy 0.000 claims abstract description 6
- 239000008280 blood Substances 0.000 claims abstract description 6
- 102000053723 Angiotensin-converting enzyme 2 Human genes 0.000 claims abstract description 5
- 108090000975 Angiotensin-converting enzyme 2 Proteins 0.000 claims abstract description 5
- 108010061994 Coronavirus Spike Glycoprotein Proteins 0.000 claims abstract description 3
- 238000002156 mixing Methods 0.000 claims description 16
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 16
- 239000002244 precipitate Substances 0.000 claims description 15
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 11
- 108091005609 SARS-CoV-2 Spike Subunit S1 Proteins 0.000 claims description 11
- 229940098773 bovine serum albumin Drugs 0.000 claims description 11
- 239000002253 acid Substances 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000003365 glass fiber Substances 0.000 claims description 8
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000001509 sodium citrate Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 7
- 229940038773 trisodium citrate Drugs 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 6
- 108010001857 Cell Surface Receptors Proteins 0.000 claims description 4
- 239000010931 gold Substances 0.000 claims description 4
- 229910052737 gold Inorganic materials 0.000 claims description 4
- 229960005486 vaccine Drugs 0.000 claims description 4
- 108010067755 dinitrophenyl-bovine serum albumin Proteins 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 2
- 238000004811 liquid chromatography Methods 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 102000006240 membrane receptors Human genes 0.000 claims 2
- 239000000463 material Substances 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 239000007790 solid phase Substances 0.000 abstract description 2
- 108010008595 sarcoma-associated antigen S1 Proteins 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 22
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 241000700605 Viruses Species 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- 102000000844 Cell Surface Receptors Human genes 0.000 description 2
- 102100031673 Corneodesmosin Human genes 0.000 description 2
- 101710139375 Corneodesmosin Proteins 0.000 description 2
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 2
- 229920003081 Povidone K 30 Polymers 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000012898 sample dilution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 208000025721 COVID-19 Diseases 0.000 description 1
- 241001678559 COVID-19 virus Species 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229940096437 Protein S Drugs 0.000 description 1
- 101710198474 Spike protein Proteins 0.000 description 1
- 101800000904 Spike protein S1 Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940080237 sodium caseinate Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nanotechnology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
编号 | 数值比 | 百分率(95%可信区间) |
阳性符合率 | 168/172 | 97.67%(94.15%-99.36%) |
阴性符合率 | 63/67 | 94.03%(85.41%-98.35%) |
阳性预期值 | 168/172 | 97.67%(94.15%-99.36%) |
阴性预期值 | 63/67 | 94.03%(85.41%-98.35%) |
总符合率 | 231/239 | 96.65%(93.51%-98.54%) |
编号 | 统计值 |
Kappa值 | 0.9170,一致性较好 |
标注误Se(K) | 0.0288 |
95%可信区间 | 0.8606-0.9735 |
标注误Se0(K) | 0.065 |
检验值Z | Z=14.1771,概率值P=0.0000 |
检验结果 | P<0.05,拒绝HO,Kappa值来自非0的总体 |
Claims (11)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111316008.5A CN114236119A (zh) | 2021-11-08 | 2021-11-08 | 新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111316008.5A CN114236119A (zh) | 2021-11-08 | 2021-11-08 | 新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114236119A true CN114236119A (zh) | 2022-03-25 |
Family
ID=80748726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111316008.5A Pending CN114236119A (zh) | 2021-11-08 | 2021-11-08 | 新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114236119A (zh) |
Citations (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102680689A (zh) * | 2011-03-10 | 2012-09-19 | 王迎峰 | 检测肌酸激酶同功酶的试剂盒及其制备和使用方法 |
CN103901216A (zh) * | 2014-04-22 | 2014-07-02 | 上海科华生物工程股份有限公司 | 人h-fabp胶体金检测试纸及其制备方法 |
CN105486859A (zh) * | 2015-11-20 | 2016-04-13 | 润和生物医药科技(汕头)有限公司 | 一种新型改进免疫层析试纸条及其制备和应用 |
CN106290829A (zh) * | 2016-07-19 | 2017-01-04 | 杭州博拓生物科技股份有限公司 | 一种四合一联合检测试纸盒及制备工艺 |
CN107688090A (zh) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | 一种NTx检测试纸、试剂盒及其制备方法 |
CN109813893A (zh) * | 2019-01-28 | 2019-05-28 | 深圳市亚辉龙生物科技股份有限公司 | Cea检测试纸条及其制备方法、cea检测装置 |
CN110824177A (zh) * | 2019-09-11 | 2020-02-21 | 润和生物医药科技(汕头)有限公司 | 一种快速检测试纸及其制备方法和应用 |
CN111624336A (zh) * | 2020-06-28 | 2020-09-04 | 宁波美丽人生医药生物科技发展有限公司 | 新型冠状病毒IgG/IgM抗体检测试剂盒及其应用方法 |
CN111693714A (zh) * | 2020-06-04 | 2020-09-22 | 华东理工大学 | 新型冠状病毒IgM/IgG抗体检测试剂盒及其应用方法 |
CN111856003A (zh) * | 2020-02-04 | 2020-10-30 | 潍坊市康华生物技术有限公司 | 一种新型冠状病毒(2019-nCoV)IgM、IgG抗体检测试纸条、试剂盒及方法 |
CN211905393U (zh) * | 2020-09-22 | 2020-11-10 | 正元盛邦(天津)生物科技有限公司 | 新型冠状病毒特异性抗体和中和抗体联合检测试纸及装置 |
CN112326973A (zh) * | 2020-12-31 | 2021-02-05 | 北京金沃夫生物工程科技有限公司 | 一种用于检测新型冠状病毒抗体的试剂盒及其应用 |
CN112362870A (zh) * | 2021-01-14 | 2021-02-12 | 山东康华生物医疗科技股份有限公司 | 登革热NS1抗原、IgG/IgM抗体两联检测试纸条、检测卡及试剂盒 |
CN112362869A (zh) * | 2021-01-14 | 2021-02-12 | 山东康华生物医疗科技股份有限公司 | 一种多项呼吸道抗原检测卡及试剂盒 |
CN112485436A (zh) * | 2020-11-09 | 2021-03-12 | 桂林电子科技大学 | 检测新冠疫苗注射后体内中和抗体的胶体金免疫层析试纸条及其制备方法 |
CN112730854A (zh) * | 2020-12-28 | 2021-04-30 | 杭州新脉生物科技有限公司 | 一种排卵检测试纸条及检测方法 |
CN112858675A (zh) * | 2021-01-05 | 2021-05-28 | 杭州奥泰生物技术股份有限公司 | 一种新型冠状病毒抗原、抗体联合智能化检测装置 |
CN113009132A (zh) * | 2021-02-10 | 2021-06-22 | 天津中新科炬生物制药股份有限公司 | 高灵敏的新型冠状病毒中和抗体检测试剂盒及检测方法 |
CN113156131A (zh) * | 2021-04-23 | 2021-07-23 | 深圳市爱康试剂有限公司 | 新型冠状病毒(covid-19)中和抗体检测试剂盒及检测方法 |
CN113295865A (zh) * | 2020-06-05 | 2021-08-24 | 南京金斯瑞生物科技有限公司 | 检测SARS-CoV-2病毒中和抗体的免疫层析装置及其应用 |
-
2021
- 2021-11-08 CN CN202111316008.5A patent/CN114236119A/zh active Pending
Patent Citations (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102680689A (zh) * | 2011-03-10 | 2012-09-19 | 王迎峰 | 检测肌酸激酶同功酶的试剂盒及其制备和使用方法 |
CN103901216A (zh) * | 2014-04-22 | 2014-07-02 | 上海科华生物工程股份有限公司 | 人h-fabp胶体金检测试纸及其制备方法 |
CN105486859A (zh) * | 2015-11-20 | 2016-04-13 | 润和生物医药科技(汕头)有限公司 | 一种新型改进免疫层析试纸条及其制备和应用 |
CN106290829A (zh) * | 2016-07-19 | 2017-01-04 | 杭州博拓生物科技股份有限公司 | 一种四合一联合检测试纸盒及制备工艺 |
CN107688090A (zh) * | 2017-07-17 | 2018-02-13 | 润和生物医药科技(汕头)有限公司 | 一种NTx检测试纸、试剂盒及其制备方法 |
CN109813893A (zh) * | 2019-01-28 | 2019-05-28 | 深圳市亚辉龙生物科技股份有限公司 | Cea检测试纸条及其制备方法、cea检测装置 |
CN110824177A (zh) * | 2019-09-11 | 2020-02-21 | 润和生物医药科技(汕头)有限公司 | 一种快速检测试纸及其制备方法和应用 |
CN111856003A (zh) * | 2020-02-04 | 2020-10-30 | 潍坊市康华生物技术有限公司 | 一种新型冠状病毒(2019-nCoV)IgM、IgG抗体检测试纸条、试剂盒及方法 |
CN111693714A (zh) * | 2020-06-04 | 2020-09-22 | 华东理工大学 | 新型冠状病毒IgM/IgG抗体检测试剂盒及其应用方法 |
CN113295865A (zh) * | 2020-06-05 | 2021-08-24 | 南京金斯瑞生物科技有限公司 | 检测SARS-CoV-2病毒中和抗体的免疫层析装置及其应用 |
CN111624336A (zh) * | 2020-06-28 | 2020-09-04 | 宁波美丽人生医药生物科技发展有限公司 | 新型冠状病毒IgG/IgM抗体检测试剂盒及其应用方法 |
CN211905393U (zh) * | 2020-09-22 | 2020-11-10 | 正元盛邦(天津)生物科技有限公司 | 新型冠状病毒特异性抗体和中和抗体联合检测试纸及装置 |
CN112485436A (zh) * | 2020-11-09 | 2021-03-12 | 桂林电子科技大学 | 检测新冠疫苗注射后体内中和抗体的胶体金免疫层析试纸条及其制备方法 |
CN112730854A (zh) * | 2020-12-28 | 2021-04-30 | 杭州新脉生物科技有限公司 | 一种排卵检测试纸条及检测方法 |
CN112326973A (zh) * | 2020-12-31 | 2021-02-05 | 北京金沃夫生物工程科技有限公司 | 一种用于检测新型冠状病毒抗体的试剂盒及其应用 |
CN112858675A (zh) * | 2021-01-05 | 2021-05-28 | 杭州奥泰生物技术股份有限公司 | 一种新型冠状病毒抗原、抗体联合智能化检测装置 |
CN112362870A (zh) * | 2021-01-14 | 2021-02-12 | 山东康华生物医疗科技股份有限公司 | 登革热NS1抗原、IgG/IgM抗体两联检测试纸条、检测卡及试剂盒 |
CN112362869A (zh) * | 2021-01-14 | 2021-02-12 | 山东康华生物医疗科技股份有限公司 | 一种多项呼吸道抗原检测卡及试剂盒 |
CN113009132A (zh) * | 2021-02-10 | 2021-06-22 | 天津中新科炬生物制药股份有限公司 | 高灵敏的新型冠状病毒中和抗体检测试剂盒及检测方法 |
CN113156131A (zh) * | 2021-04-23 | 2021-07-23 | 深圳市爱康试剂有限公司 | 新型冠状病毒(covid-19)中和抗体检测试剂盒及检测方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2853894B1 (en) | Diagnostic kits and immunoassay methods for diagnosis and differentiation of African Swine Fever Virus (ASFV) and Classical Swine Fever Virus (CSFV) | |
CN111562369A (zh) | SARS-CoV-2中和抗体检测试剂盒 | |
CN111474354B (zh) | 2019-nCoV新型冠状病毒检测层析试纸条、检测卡和试剂盒 | |
CN111337689A (zh) | 一种新型冠状病毒检测试剂盒 | |
CN111879933A (zh) | 检测新型冠状病毒的免疫层析试纸 | |
CN111273003A (zh) | 2019-nCoV新型冠状病毒快速检测免疫层析试纸条 | |
CN113702643B (zh) | 一种联合检测新型冠状病毒中和抗体及核衣壳蛋白抗体的装置 | |
CN113295865B (zh) | 检测SARS-CoV-2病毒中和抗体的免疫层析装置及其应用 | |
CN111896747A (zh) | 检测新型冠状病毒IgM和IgG的免疫层析试纸 | |
EP4249508A1 (en) | Sars-cov-2 detection kit and sars-cov-2 detection method | |
CN112305218A (zh) | 一种新型冠状病毒抗体胶体金免疫侧向层析检测方法及其应用 | |
CN106596937A (zh) | 一种同步检测胰腺癌标记物的量子点试剂盒 | |
CN113567666A (zh) | 一种荧光微球标记的免疫层析法新型冠状病毒检测试纸条及其制备方法和应用 | |
CN112904001A (zh) | 新冠及甲乙型流感病毒抗原联检试剂盒及其制备方法与应用 | |
WO2005062052A1 (ja) | 簡易メンブレンアッセイ法及びキット | |
CN114236119A (zh) | 新冠总抗体和中和抗体胶体金快速检测试纸及其制备方法 | |
CN111856008A (zh) | 一种多种呼吸道病原体快速检测试纸及其制备方法 | |
CN101706499A (zh) | Flag融合标签胶体金检测试纸条及其制备方法 | |
CN114252601A (zh) | 一种新冠总抗体和中和抗体乳胶快速检测试纸及制备方法 | |
CN113391075B (zh) | 一种新型冠状病毒中和抗体检测试纸条和试剂盒 | |
CN106053824A (zh) | 胰腺癌双联检测试剂盒 | |
CN102980997B (zh) | EB病毒衣壳抗原IgM抗体胶体金法检测试剂及其制备方法 | |
CN215066713U (zh) | 一种新型冠状病毒中和抗体检测试纸和检测装置 | |
WO2022160377A1 (zh) | 一种新型冠状病毒中和抗体检测试纸 | |
CN107102132A (zh) | 量子点荧光探针及其制备方法、免疫层析试纸 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant after: Guangzhou Iconas Biomedical Technology Co.,Ltd. Address before: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant before: Guangzhou Iconas Biomedical Technology Co.,Ltd. Address after: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant after: Guangzhou Iconas Biomedical Technology Co.,Ltd. Address before: 515063 Rongsheng science and Technology Park, University Road, Tuolian street, Jinping District, Shantou City, Guangdong Province Applicant before: RUNBIO BIOTECH Co.,Ltd. |
|
CI02 | Correction of invention patent application | ||
CI02 | Correction of invention patent application |
Correction item: Applicant|Address Correct: RUNBIO BIOTECH Co.,Ltd.|515063 Rongsheng science and Technology Park, University Road, Tuolian street, Jinping District, Shantou City, Guangdong Province False: Guangzhou Iconas Biomedical Technology Co.,Ltd.|Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Number: 08-01 Volume: 39 |
|
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant after: Guangdong Iconas Biomedical Technology Co.,Ltd. Address before: Inside Rongsheng Science and Technology Park, Daxue Road, Tuolian Street, Jinping District, Shantou City, Guangdong Province, 510801 Applicant before: RUNBIO BIOTECH Co.,Ltd. Address after: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant after: Guangdong Iconas Biomedical Technology Co.,Ltd. Address before: Room 116, Floor 1, Transportation Bureau Building, No. 95, Yingbin Avenue, Huacheng Street, Huadu District, Guangzhou, Guangdong Province, 510801 Applicant before: Guangdong Iconas Biomedical Technology Co.,Ltd. |
|
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220325 |